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61.
REN Yu-sheng WU Zong-gui CUI Fang JIA Guo-liang YU Shi-qing TANG Chao-wu LI Bo 《园艺学报》2002,18(11):1377-1380
AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using [3H]-TdR incorporation and [3H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts. 相似文献
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ZHANG Yu-xia YU Lun-yin LIU Ming-qiu ZHANG Zheng-bin TANG Zhi-jiao XIA Dong WANG Ming 《园艺学报》2002,18(1):32-35
AIM:To investigate the protein expression of cyclin D2 and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS:One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D2 and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS:①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D2 in 3,4,5 day CM group was 0.89 times(P<0.05),0.80 times (P<0.05) and 0.56 times (P<0.01) of that in 1 day group, respectively. The expression of p16 in CM was increased during the culture process, 2,3,4,5 day group were 1.63 times, 1.72 times, 1.99 times and 2.84 times (P<0.01) of that in 1 day group, respectively.CONCLUSION:Cultured neonatal rat cardiac myocytes could proliferate during the first 3 days after incubation, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D2 and p16 play the key roles in CM postnatal development. Downregulation of cyclin D2 and upregulation of p16 may induce CM differentiation. 相似文献
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本文综述了对螟虫长距茧蜂的研究概况,包括该寄生蜂的主要生物学习性、对玉米螟自然寄生率及田间防治玉米螟效果等。 相似文献
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生姜根结线虫病原鉴定及发生规律 总被引:5,自引:1,他引:5
采用田间调查、接种试验、电镜与显微镜观察以及酯酶同工酶电泳等方法,对引发生姜癞皮病的病原及发生规律进行了研究.结果表明,引起生姜癞皮病的病原为南方根结线虫Meloidogyne incognita.该病在每年6月中旬开始发生,8、9月份危害严重.病原在生姜上一年可发生完整的4代,完成1代平均约需35天.病原主要在0~40cm的土层内分布和危害,但具体分布情况依寄主生长状况而稍有差异.南方根结线虫繁殖速率受初始接种密度的影响也很大,当初始接种密度较低时,线虫繁殖速率较高,初始接种密度增大,繁殖速率降低,其平衡密度为每100g干土746.20个卵. 相似文献
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