Colonization,proliferation, and survival of intraperitoneally transplanted yellowtail <Emphasis Type="Italic">Seriola quinqueradiata</Emphasis> spermatogonia in nibe croaker <Emphasis Type="Italic">Nibea mitsukurii</Emphasis> recipient     

Kentaro Higuchi  Yutaka Takeuchi  Misako Miwa  Yoji Yamamoto  Kazunobu Tsunemoto  Goro Yoshizaki 《Fisheries Science》2011,77(1):69-77

Recently, we developed an intraspecies spermatogonial transplantation technique in a pelagic egg spawning marine teleost, nibe croaker Nibea mitsukurii. Nibe croaker is an ideal candidate recipient for spermatogonial transplantation since it has a short generation time and small body size. In the present study, yellowtail Seriola quinqueradiata spermatogonia were transplanted into nibe croaker larvae, and the behavior of transplanted spermatogonia in recipient gonads was observed. Three weeks post-transplantation, yellowtail spermatogonia were incorporated into the gonads of 72 out of 88 recipients. An antiproliferating cell nuclear antigen was detected in incorporated yellowtail spermatogonia, suggesting that the xenogenic germ cells were proliferating in recipient gonads. Yellowtail vasa-positive spermatogonia survived for 11 months after transplantation in the gonads of recipient fish. Thus, we showed that the microenvironment in nibe croaker gonads can support the colonization, proliferation, and survival of germ cells derived from a different taxonomic family.  相似文献   

Biological characteristics of fish germ cells and their application to developmental biotechnology     

G Yoshizaki  T Okutsu  T Morita  M Terasawa  R Yazawa  Y Takeuchi 《Reproduction in domestic animals = Zuchthygiene》2012,47(Z4):187-192

We have revealed several unique characteristics of germ cell development using rainbow trout, including the fact that spermatogonia transplanted into the peritoneal cavity of newly hatched embryos migrate toward recipient gonads, that spermatogonia transplanted into female recipients start oogenesis and produce functional eggs and that diploid germ cells transplanted into triploid trout can complete gametogenesis. By combining these unique features of fish germ cells, we established allogeneic and xenogeneic transplantation systems for spermatogonia in several fish species. Spermatogonia isolated from the mature testes of vasa-green fluorescent protein (Gfp) transgenic rainbow trout were transplanted into the peritoneal cavity of triploid masu salmon newly hatched embryos. These spermatogonia migrated toward recipient salmon genital ridges with extending pseudopodia and were subsequently incorporated into them. We further confirmed that the donor-derived spermatogonia resumed gametogenesis and produced sperm and eggs in male and female salmon recipients, respectively. By inseminating the resulting eggs and sperm, we obtained only rainbow trout offspring in the F1 generation, suggesting that the triploid salmon recipients produced functional gametes derived only from donor trout. We further confirmed that this intra-peritoneal transplantation of germ cells is applicable to several marine fishes, which could be of benefit in the production of bluefin tuna that has a large broodstock (>100 kg) and is difficult to maintain in captivity. Gamete production of bluefin tuna could be more easily achieved by generating a surrogate species, such as mackerel, that can produce tuna gametes.  相似文献   

赤霉素涂抹法对葡萄果实的无核化及果实膨大的影响   总被引：5，自引：0，他引：5  

廖康  伏见拓也  冈本五郎 《新疆农业大学学报》2002,25(1):27-30

本试验用羊毛脂制成的糊状赤霉素涂抹处理里扎马特、巨峰和先锋葡萄的果梗进行无核化研究 ,试验结果表明 :赤霉素涂抹葡萄果梗后可使葡萄果实无核化并使果实膨大 ,尤其是先锋葡萄可通过一次处理达到较好的无核效果 ,获得的无核果实较对照果实大并且品质优良。涂抹法较水溶液处理法简单、方便 ,有利于在生产上应用。本试验的结果浓度在 10 0～ 30 0 0mg·L-1范围内均有较好的效果  相似文献   

糙米的厚层微波干燥   总被引：4，自引：0，他引：4       下载免费PDF全文

董铁有  朱文学  木村俊范  吉崎繁 《农业工程学报》2003,19(2):160-162

研究了顺流通风状态下厚层糙米的微波干燥问题，实验结果表明：随着微波功率的增加，糙米的温度和干燥速度随之增加。如果微波的功率被控制在0.05～0.09 kW/kg范围内，风速被控制在0.12～0.20 m/s范围内，则可以保证不出现爆腰和发芽率降低等质量问题。且糙米的有效干燥厚度大约为0.130 m，此值大于同等条件下稻谷的有效干燥厚度。在微波加热的条件下干燥糙米的效率要高于稻谷干燥的效率。  相似文献   

Annual periodicity of fruiting in temperate forests in Yakushima,Japan     

Goro HANYA  Shin-ichiro AIBA 《中国林学(英文版)》2011,13(2):112-122

Fruiting phenology, assessed by seed fall, in five warm-and cool-temperate forests on Yakushima Island, southern Japan, was studied for two years in one 50 m × 50 m plot and for four years in four 100 m × 50 m plots. The elevation of the plots ranged between 170 and 1200 m a.s.l. Seed fall phenology showed annual periodicity in all five plots. This was clear when assessed by the number of species but became less clear when assessed by the biomass of seed litter. Community-level annual periodicity was based on the prevalence of population-level annual periodicity and interspecific synchronization of the fruiting peak from autumn to winter. Fleshy fruits had peaks of seed fall in a wider range of months than non-fleshy fruits, since it is sometimes beneficial to bear fruit outside the community-level fruiting peaks in order to avoid interspecific competition for animal seed dispersers. No consistent effect of climatic factors on seed fall phenology was detected.  相似文献   

Production of biologically-active recombinant goldfish gonadotropins in transgenic rainbow trout     

T. Morita  G. Yoshizaki  M. Kobayashi  T. Takeuchi 《Fish physiology and biochemistry》2003,28(1-4):473-474

The feasibility of using rainbow trout Oncorhynchus mykiss embryos as an expression system for proteins was investigated. For model proteins, we selected two goldfish gonadotropins (GTHs), follicle-stimulating hormone (FSH) and luteinizing hormone (LH). To produce single-chain goldfish FSH (scgfFSH) and LH (scgfLH), cDNAs encoding glycoprotein hormone (GP) α and FSHβ were fused in tandem, and cDNAs encoding GPα and LHβ were fused in tandem. The fused cDNAs were ligated with β-actin promoter, and microinjected into fertilized rainbow trout eggs. After 4-days incubation, the embryos were subjected to western blotting and in vitro bioassays. The recombinant proteins produced by the embryos were immunoreactive to antisera against goldfish GPα, N-glycosylated, and biologically active. We conclude that scgfFSH and scgfLH were successfully produced in transgenic rainbow trout.  相似文献   

Cryopreservation of trout primordial germ cells   总被引：2，自引：0，他引：2  

T. Kobayashi  Y. Takeuchi  G. Yoshizaki  T. Takeuchi 《Fish physiology and biochemistry》2003,28(1-4):479-480

A method for cryopreserving trout primordial germ cells (PGCs) was established. Trout PGCs were successfully cryopreserved with high survival by freezing with medium containing 1.8 M ethylene glycol. Transplantation experiment revealed that frozen/thawed PGCs colonized and proliferated in the recipients' gonads.  相似文献   

Spontaneous hybridization in the laboratory and genetic markers for the identification of hybrids between two atherinid species, Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909)     

C A Strüssmann  T Akaba  K Ijima  K Yamaguchi  G Yoshizaki  F Takashima 《Aquaculture Research》1997,28(4):291-300

This study reports the occurrence of spontaneous hybridization between Odontesthes bonariensis (Valenciennes, 1835) and Patagonina hatcheri (Eigenmann, 1909), two South American freshwater atherinids, while adults of both species were being held in a communal laboratory tank, and describes suitable genetic markers for distinction of both species and their hybrids. Combined allozyme and mitochondrial DNA restriction length polymorphism analyses of offspring from three spawnings in the communal tanks confirmed the presence of hybrids along with purebreds in each spawning. Hybrids of an O. bonariensis mother X a P. hatcheri father were produced along purebred O. bonariensis in one occasion, whereas hybrids of P. hatcheri mothers X O. bonariensis fathers were produced along purebred P. hatcheri in two occasions. Three enzyme systems (coded by loci LDH-B*, PGM*, and CK*) out of 15 (20 loci) screened allowed distinction of species and their hybrids, but the usefulness of LDH-B* for field work may be limited by intraspecific polymorphism. Eight restriction enzymes (Apa I, Bgl II, Eco RI, Eco RV, Eco T22I, Hinc II, Hind III, and Pvu II) among 11 produced diagnostic restriction fragments applicable for species (motherhood) distinction. The relevance of these findings for the management of natural genetic resources and the aquaculture of these two species is discussed.  相似文献   

Simulating and mapping agricultural land productivity: An application to Macadamia nut     

Tung Liang  Wilbert P.H. Wong  Goro Uehara 《Agricultural Systems》1983,11(4):225-253

A procedure for simulating and mapping land productivity was developed. The procedure used only existing data such as soil surveys based on soil taxonomy, weather records and yield either from published sources or collected from producers. It provides a means of assessing quantitatively the likelihood of introducing new crops to a region. The need to estimate benefits from crop inputs modification such as irrigation and fertilizer can also be met by this procedure. It can also be used to help select crop performance test sites for maximum extrapolation of test results to a large area.  相似文献   

Artificial seedlings of Sargassum macrocarpum developed from adventive embryos     

Goro Yoshida  Koji Yoshikawa and  Toshinobu Terawaki 《Fisheries Science》2001,67(2):352-354

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