小肽对鲤鱼免疫力的影响   总被引：15，自引：1，他引：15  

李清  肖调义  毛华明 《饲料研究》2005,(5):1-3

选用1 80尾体质量相近、健康的成鲤作饲养试验,随机分成4组,设对照组1个,试验组3个(日粮中分别添加0 .3 %、0 .6 %、1 .0 %的小肽)。试验结束后,观察在基础日粮中添加不同量的小肽对成鲤血液生理生化指标及脏体器官的影响,以探讨小肽对鲤鱼免疫力的影响。结果表明,1 )试验组成鲤血清中的免疫球蛋白(IgM)和补体(C4)的浓度均高于对照组(P <0 .0 5) ,其中1 .0 %小肽组的IgM和C4分别为450 .81mg/L和1 7.69mg/L ,比对照组提高1 4 .6 %和95 .7%,补体(C3 )差异不显著(P >0 .0 5) ;尿素氮(BUN)含量比对照组低(P <0 .0 5) ,尤其是1 .0 %小肽组比对照组降低2 7.9%;2 )试验组成鲤的内脏比、肝体比和脾体比均比对照组提高,但差异不显著(P >0 .0 5) ,随着饵料中小肽用量的增加肾体比也逐渐提高,1 .0 %小肽组的肾体比比对照组提高56.3 %(P <0 .0 5)。  相似文献   

Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene          下载免费PDF全文

Yang Zhao  Yuedong Xuan  Daolin Mou  Hong Liu  Pan Zhou  Zhengfeng Fang  Lianqiang Che  Shengyu Xu  Bin Feng  Jian Li  Xuemei Jiang  Yan Lin  De Wu 《Journal of animal physiology and animal nutrition》2018,102(5):1340-1350

The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB₁₂, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor.  相似文献   

云贵川三省部分地区警犬狂犬病血清中和抗体调查     

杜晓鹏  刘婷芳  徐陶  王宇洋  毛爱国  许炜迪  张垚  王雄  党西锋  张明飞  冯烨 《中国动物检疫》2018,35(3):25-29

为评价警犬的狂犬病免疫效果,了解影响免疫效果的因素,2017年在云南、贵州、四川三省部分地区免疫狂犬病疫苗的警犬中随机抽样,采用荧光抗体病毒中和试验(FAVN),检测血清中狂犬病病毒中和抗体效价。结果显示,共抽检187只警犬,中和抗体阳性127只(≥0.50 IU/m L),总体免疫合格率为67.91%,其中云南、贵州、四川3省的免疫合格率分别为57.14%、68.33%、76.06%。结果分析表明,警犬狂犬病免疫合格率与犬的年龄、免疫次数、疫苗类型等因素有关,而与性别和品种无关。建议完善警犬免疫程序,加大血清中和抗体监测力度,对不合格警犬及时加强免疫;对每个影响因素进行连续性分析,以确定影响警犬狂犬病免疫合格率的具体因素。  相似文献   

草鱼呼肠孤病毒生长特性研究     

袁雪梅  姚嘉赟  郝贵杰  蔺凌云  徐洋  潘晓艺  尹文林  沈锦玉 《中国动物检疫》2018,35(3):98-103

以草鱼呼肠孤病毒(GCRV)873毒株为试验材料,建立了GCRV荧光定量PCR检测方法;用GCRV873株感染CIK细胞及草鱼,通过对病毒RNA复制水平、子代病毒含量以及细胞病变等情况进行监测,研究其在CIK细胞及草鱼体内的生长特性。在CIK细胞中,GCRV感染后12 h即可检测到子代病毒,36 h时,所有细胞被感染;72 h时,病毒滴度达到最高,为10~(6.75) TCID_(50)/mL。在草鱼的肝、脾、肾中,均能检测到GCRV RNA,其中肾脏中含量最高,其次是肝脏,脾脏中最少。本试验为研究GCRV致病机理及制备细胞灭活疫苗奠定了试验基础。  相似文献   

Effect of Different Concentrate-roughage Ratios on Rumen Degradability of Cassava Stems and Leaves Total Mixed Ration in Goat   总被引：1，自引：0，他引：1  

HU Lin  WANG Ding-fa  LI Wei  ZHOU Lu-li  LI Mao  ZHOU Han-lin 《中国畜牧兽医》2016,43(11):2914-2921

The rumen degradations of TMR were studied in this experiment,in which cassava stems and leaves were used as roughage and formulated with concentrate in different proportions.The purpose of this study was to formulate the available TMR for finishing goat,and provide a theoretical basis for the applications of cassava stems and leaves in goat production.Six Hainan Black goats with permanent rumen fistulas were selected,the rumen degradabilities of DM,CP,NDF and ADF of TMR with 6 different concentrate to forage ratio（2:8,3:7,4:6,5:5,6:4 and 7:3）were tested using nylon bag method.The results showed that,the DM effective degradabilities at the ratio of 4:6,5:5,6:4,7:3 were significantly higher than that of 2:8 and 3:7（P<0.05).CP effective degradabilities at the ratio of 2:8,3:7,4:6 were significantly higher than that of 5:5,6:4 and 7:3（P<0.05).The NDF effective degradation at the ratio of 5:5 was significantly higher than that in the other groups（P<0.05).The ADF degradation at the ratio of 3:7 was significantly higher than that of 4:6,5:5, 6:4 and 7:3（P<0.05).The results indicated that,the nutrient degradation in rumen were higher when the concentrate to forage（cassava stems and leaves）ratio in TMR were 3:7,4:6 and 5:5,and the recommended ratio range were 4:6 to 5:5 in production practice.  相似文献   

TFSQ型高效环保组合式粮食杂质清理筛的研制     

毛根武  杨健  周清伟  董德良  肖红  向飞 《粮食储藏》2014,(2):50-53

通过对振动筛理、垂直风选、旋风分离与沉降系统的研究,研制出集振动筛理、风选、旋风分离沉降、含尘气流循环再利用为一体的TFSQ型高效环保组合式粮食杂质清理筛。经应用测试和专家鉴定:该设备具有清理效率高、清理过程粉尘污染小,使用维护方便、可靠性强等优点,具有广阔的市场推广前景。  相似文献   

Identification of Differentially Expressed Genes and Lipid Metabolism Signaling Pathways between Muscle and Fat Tissues in Broiler Chickens     

Min Zhang  Dan Zheng  Zhimei Peng  Yuting Zhu  Rongrong Li  Qiong Wu  Yan Li  Hongyi Li  Weihua Xu  Mao Zhang  Qichun Hung  Yanfa Sun 《The Journal of Poultry Science》2021,58(2):131

In this study, signaling pathways and key differentially expressed genes (DEGs) involved in lipid metabolism in muscle and fat tissues were investigated. Muscle and abdominal fat tissues were obtained from 35-day-old female broilers for RNA sequencing. DEGs between muscle and fat tissues were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses of DEGs were performed. A total of 6130 DEGs were identified to be significantly enriched in 365 GO terms, most of which were involved in biological processes, cellular components, and molecular functions in muscle and fat tissues. Three important lipid signaling pathways (pyruvate metabolism, the insulin signaling pathway, and the adipocytokine signaling pathway) were identified among the fat and muscle tissues of broilers. The key common DEGs in these pathways included phosphoenolpyruvate carboxykinase 2 (PCK2), acetyl-CoA carboxylase 1 alpha and beta (ACACA and ACACB), and the mitogen-activated protein kinase (AMPK) gene family. Hence, our findings revealed the pathways and key genes and gene families involved in the regulation of fat deposition in the muscle and fat tissues of broilers.  相似文献   

我国竞技体育人才培养路径的协同机制研究*     

陈道裕  赵国华  张丹丹  叶〓茂 《上海交通大学学报(农业科学版)》2015,(5):1-3

体工队“三级训练网”、俱乐部、院校化是我国竞技体育人才培养的三个主要路径。研究运用文献研究、专家访谈等方法，解析各个培养路径的机制结构,分析不同培养路径的矛盾问题和解决思路，在培养主体、培养目标、人才输送三个方面提出我国竞技体育人才培养路径的协同机制。  相似文献   

煮熟和干燥处理对黄藤笋抗氧化活性的影响     

黄世能  黄雪梅  牛国才  张昭其 《世界竹藤通讯》2014,(3):1-4

通过测定黄藤熟笋、生笋(鲜切笋)、熟笋干和生笋干的水和4种不同浓度乙醇提取物中的自由基清除能力、总还原能力、总多酚含量和总黄酮含量,研究了煮熟、干燥加工方法对黄藤笋提取物抗氧化活性的影响。结果表明,煮熟处理能保持或提高提取物的抗氧化活性,干燥处理则相反。乙醇提取物比水提取物具有更高的抗氧化活性,以50%~75%的乙醇提取溶剂效果最好。熟笋在所有溶剂中的自由基清除能力、总还原能力、总多酚含量和总黄酮含量均表现出最高水平,表明煮熟是黄藤笋适宜的加工利用方式。  相似文献   

郑州市城市绿地人工群落调查及物种多样性分析     

毛洁  郭欢欢 《河南林业科技》2017,37(2)

以郑州市公园绿地、附属绿地中的120个人工植物群落为研究对象,调查其群落结构和物种多样性,利用Margalef丰富度指数、Shannon-Wiener多样性指数、Simpson多样性指数和Pielou均匀度指数对郑州市城市绿地人工群落的物种多样性进行研究,结果表明郑州市城市绿地人工群落的丰富度指数均处于较高水平,植物多样性丰富,其中公园绿地丰富度指数最高,道路附属绿地次之,社区附属绿地最低。  相似文献