Review of infectious agent occurrence in wild salmonids in British Columbia,Canada     

Beibei Jia  Marina K. V. C. Delphino  Babafela Awosile  Tim Hewison  Patrick Whittaker  Diane Morrison  Mykolas Kamaitis  Ahmed Siah  Barry Milligan  Stewart C. Johnson  Ian A. Gardner 《Journal of fish diseases》2020,43(2):153-175

Wild Pacific salmonids (WPS) are economically and culturally important to the Pacific North region. Most recently, some populations of WPS have been in decline. Of hypothesized factors contributing to the decline, infectious agents have been postulated to increase the risk of mortality in Pacific salmon. We present a literature review of both published journal and unpublished data to describe the distribution of infectious agents reported in wild Pacific salmonid populations in British Columbia (BC), Canada. We targeted 10 infectious agents, considered to potentially cause severe economic losses in Atlantic salmon or be of conservation concern for wild salmon in BC. The findings indicated a low frequency of infectious hematopoietic necrosis virus, piscine orthoreovirus, viral haemorrhagic septicaemia virus, Aeromonas salmonicida, Renibacterium salmoninarum, Piscirickettsia salmonis and other Rickettsia-like organisms, Yersinia ruckeri, Tenacibaculum maritimum and Moritella viscosa. No positive results were reported for infestations with Paramoeba perurans in peer-reviewed papers and the DFO Fish Pathology Program database. This review synthesizes existing information, as well as gaps therein, that can support the design and implementation of a long-term surveillance programme of infectious agents in wild salmonids in BC.  相似文献   

Partial replacement of dietary soybean meal by high‐protein distiller's dried grains (HPDDG) supplemented with protease enzyme for European seabass,Dicentrarchus labrax fingerlings     

Ashraf M. A.‐S. Goda  Sherine R. Ahmed  Hany M. Nazmi  Mohamed Z. Baromh  Kevin Fitzsimmons  Waldemar Rossi  Simon Davies  Ehab El‐Haroun 《Aquaculture Nutrition》2020,26(3):842-852

High‐protein distillers dried grains (HPDDG) is a co‐product of ethanol production that uses prefractionation technology. A 70‐day growth trial was conducted to investigate the effect of partial replacement of dietary soybean meal by high‐protein distiller's dried grains (HPDDG) with protease enzyme supplementation (PROXYM ULTRA^®) on growth performance, physiological parameters and histological changes of the intestine of European sea bass, Dicentrarchus labrax fingerlings. The results indicated that increased dietary HPDDG levels up to 50% of HPDDG supplemented with protease significantly increases growth performance and feed utilization and improved FCR of sea bass. In addition, replacement of SBM by HPDDG supplemented with protease enhanced feed intake efficiency and the health status of fish. Haematology and serum biochemistry (haemoglobin (Hb)), red blood cells (RBCs), white blood cells (WBCs) and humeral immune parameters including total protein, globulin, cholesterol, lysozyme activity and total antioxidant capacity significantly increased with increase in HPDDG supplemented with protease in the diets. Results of this study indicated that HPDDG supplemented with protease is a good alternative protein source for aquaculture feed and can be included up to 50% as a replacement of SBM without compromising growth performance and physiological parameters of sea bass.  相似文献   

Possible effect of lesser galangal (Alpinia officinarum) extracts encapsulated into mesoporous silica nanoparticles on the immune status of rainbow trout (Oncorhynchus n)     

Elham Awad  Ahmed El‐Fiqi  Dawn Austin  Alastair Lyndon 《Aquaculture Research》2020,51(9):3674-3684

Using plant extracts as immunostimulants in aquaculture has prove effectiv in resisting infectious disease, in addition to being safe and inexpensive. The present study is concerned about the prospective mode of action of using lesser galangal extract (Alpinia officinarum) encapsulated into amine surface functionalized mesoporous silica nanoparticles (MSN) in order to elevate the immune status of rainbow trout (Oncorhynchus mykiss). Fish were randomly divided into 6 groups and fed for 4 weeks with commercial diets supplemented with 0% (control), 0.5% and 1.5% of lesser galangal extract, and with previous concentrations encapsulated with MSN (0.5% + MSN, 1.5% + MSN) and MSN (without extract). The effects of the feeding trial on the innate humoural immune parameters (total protein, myeloperoxidase content, lysozyme antiproteases and bactericidal activities) in rainbow trout were examined. Also, the influence of dietary supplement on some immune‐related genes in rainbow trout head kidney (TNF, IL‐8, IL‐1b, LYZ2 and CD4) after challenge with Yersinia ruckeri was examined. The results revealed enhancement in immune parameters in all treatment groups compared to the control, especially in the fish group fed with 1.5% + MSN which showed the highest significant difference (p < .05) in total protein, lysozyme and antiproteases activities. Also, feeding lesser galangal extract encapsulated into MSN led to an increase in myeloperoxidase content and bactericidal activity. An improvement in the expression of immune‐related genes has been recorded in fish groups fed doses of lesser galangal extract and lesser galangal extract encapsulated into MSN compared to the control or to the group fed with MSN only. Particularly, the group fed with 0.5% + MSN showed a significant up‐regulation in most of the immune‐related genes. The current investigation supports using lesser galangal extract encapsulated into MSN in fish diets as a supplement to enhance the immune response of rainbow trout and elevate its resistance to infectious diseases.  相似文献   

Comparison of growth,digestive system maturation and skeletal development in sea bass larvae reared in an intensive or a mesocosm system     

Dora Zouiten  Ines Ben Khemis  Ahmed Slaheddin Masmoudi  Christine Huelvan  Chantal Cahu 《Aquaculture Research》2011,42(11):1723-1736

The quality of development in intensive or mesocosm hatchery‐reared Dicentrarchus labrax larvae was investigated using physiological indicators assessing ontogeny. Larvae were reared in intensive (120 L tanks) and in mesocosm systems (20 m³ enclosures) with the same feeding sequence, excluding the wild zooplankton naturally available in mesocosms. Faster growth was recorded since early development [16 day after hatching (DAH)] in the mesocosm. Maturation of the digestive system also occurred earlier as indicated by the higher amylase secretion ratios, the intestinal maturation index (alkaline phosphatase/leucine–alanine peptidase and aminopeptidase‐N/leucine–alanine peptidase ratios) and the more developed intestinal epithelium at 23 DAH. Nevertheless, the delay in digestive maturation in the intensive system seemed retrieved within few days. In both the groups, the number of vertebrae ranged between 24 and 26, with the dominant class being 25 vertebrae. However, the distributions differed between treatments for meristic characteristics, ossification stages and incidence of malformation types. Loss of a vertebra was more frequent in the intensive system, while the appearance of an additional vertebra was more frequent in the mesocosm. Ossification at 37 DAH was also more advanced in the mesocosm in addition to a lesser rate and severity of skeletal malformations. It is suggested that the early nutritional contribution of mesocosm wild zooplankton, yet at densities of 0.2–0.7 prey mL^?1, had key effects on larvae development since the early stages.  相似文献   

Evaluation of Trichoderma harzianum for controlling root rot caused by Phytophthora capsici in pepper plants   总被引：5，自引：0，他引：5  

A. Sid Ahmed  C. Pérez-Sánchez  C. Egea  & M. E. Candela 《Plant pathology》1999,48(1):58-65

The ability of Trichoderma harzianum to control the rotting of pepper ( Capsicum annuum ) plant roots caused by Phytophthora capsici was studied. Interactions between the fungi were assessed in vitro on three culture media (V8c, Czapek and 2% water agar) and in vivo in plants grown in a substrate inoculated with P. capsici and T. harzianum . Studies on mutual antagonism in vitro showed that P. capsici was inhibited by T. harzianum ; however, the intensity of inhibition differed according to the medium used, being greatest on Czapek. Analysis of the fungal populations in the plant growth substrate showed that T. harzianum consistently reduced that of P. capsici over time. This reduction in the pathogen population was associated with a reduction in root rot of between 24 and 76%, although plant growth (dry weight) was still reduced by 21.2–24.7%, compared with the uninoculated control. In the absence of T. harzianum with the same pathogen inoculum levels, the reduction in dry weight was 59.8–68.6%, suggesting that T. harzianum reduced the damage.  相似文献   

Cross-bred Cattle Protected against Hyalomma anatolicum anatolicum by Larval Antigens Purified by Immunoaffinity Chromatography     

Ghosh  S.  Khan  M.H.  Ahmed  N. 《Tropical animal health and production》1999,31(5):263-273

Antigens from larvae of Hyalomma anatolicum anatolicum were extracted and purified by immunoaffinity chromatography using immunoglobulin ligands from cross-bred animals immunized with soluble larval antigen. Affinity-purified antigen (Aff-TLE) and a total larval extract (TLE) were used to immunize cross-bred (Bos indicus×Bos taurus) cattle. The group immunized with Aff-TLE rejected 71.6% of larvae and 77.3% of nymphs. However, the rejection percentages were lower in the TLE-immunized group. No significant changes in the feeding period, moulting percentages or moulting period of engorged larvae and nymphs were recorded. There was, however, a significant decrease in the number of resultant nymphs p<0.01) and adults (p<0.01) in the ticks fed on the Aff-TLE-immunized group. The Aff-TLE antigen was 93.3% purified. SDS-PAGE analysis identified a 39 kDa protein, reported for the first time, as the antigen responsible for the induction of resistance in the host.  相似文献   

Integrating genotype,fungicide, and spray schedule reduced gall (Physoderma) disease progression and enhanced grain yield in faba bean     

Bitew  Beyene  Fininsa  Chemeda  Terefe  Habtamu  Ahmed  Seid 《Journal of Crop Science and Biotechnology》2023,26(2):135-149

Journal of Crop Science and Biotechnology - Faba bean is a cool season food legume crop. However, productivity of the crop is constrained by faba bean gall (FBG), which is an emerging disease that...  相似文献   

Effects of different carotenoid supplementation sources with or without sodium taurocholate on growth,feed utilization,carotenoid content and antioxidant status in fry of the European seabass,Dicentrarchus labrax          下载免费PDF全文

Ahmed Elsayed Sallam  Abdallah Tageldien Mansour  Tarek Mohamed Srour  Ashraf Mohamed Abdelsameè Goda 《Aquaculture Research》2017,48(7):3848-3858

A total of 210 European seabass fry with an average body weight of 0.51 ± 0.03 g were divided into seven experimental treatments (three replicates per treatment). The seven experimental diets (45.64% CP) were formulated to contain synthetic astaxanthin (SA; 0.1 g kg^?1), marigold flower meal (MFM; 0.2 g kg^?1) or crab waste meal (CM; 10 g kg^?1), with or without sodium taurocholate (ST; 0.4 g kg^?1); the control diet contained no supplementation. The experiment was conducted for 8 weeks. The results show that seabass fry fed the diets containing different sources of carotenoids, especially with ST, demonstrated higher growth efficiency, survival (%), feed efficiency, protein and ether extract contents of fish body. The best results were found with Diet_MFM+ST. Whole‐body carotenoid levels increased significantly with all supplementation treatments; the highest level was observed with SA, followed by fish fed Diet_MFM and Diet_CM, especially with ST co‐supplementation. Moreover, thiobarbituric acid‐reactive substances were significantly reduced in fish fed the diets containing different sources of carotenoids compared to the control diet. The total antioxidant status significantly increased in fish fed Diet_SA and Diet_CM alone or with ST compared to the control group. In conclusion, these results reveal that Diet_MFM+ST is the most suitable treatment for improving growth, feed efficiency, and the protein and ether extract contents of seabass fry compared to other sources of carotenoids. The best oxidant/antioxidant balance and carotenoid content were achieved using SA, CM and MFM with ST supplementation.  相似文献   

Response to the ration levels on growth,body composition,energy, and protein maintenance requirement of the Indian catfish (<Emphasis Type="Italic">Heteropneustes fossilis</Emphasis>—Bloch 1974)     

Imtiaz Ahmed 《Fish physiology and biochemistry》2010,36(4):1133-1143

An 8-week growth study was conducted to determine the effect of ration level, energy, and protein maintenance requirement of catfish, Heteropneustes fossilis–Bloch, fingerling (7.90 ± 0.55 cm; 3.10 ± 0.28 g) by feeding casein–gelatin-based purified diet (40% CP; 3.61 kcal g-1 GE) at six ration levels 1–6% of BW/day, at 0800 and 1700 h, in triplicate, with 20 fish per trough fitted with water flow-through system of volume 55 L. Maximum live weight gain, best feed conversion ratio (FCR), best specific growth rate (SGR), and highest protein efficiency ratio (PER) were evident for ration levels of 4–5% body weight. However, second-degree polynomial regression analysis for weight gain, FCR, PER, protein, and energy retention data indicated that the break-points occurred at 5.08, 4.18, 4.05, 4.16, and 4.17% BW/day, respectively. Significantly (P < 0.05) higher body protein content was recorded at 4 and 5% rations. While a linear increase in body fat content with inverse relationship in moisture content was evident with increasing rations. Ash content remained insignificantly (P > 0.05) low at higher rations. Protein and energy retention values also produced significant (P < 0.05) differences. Based on the results obtained, it is recommended that feeding in the range of 4 to 4.5% BW/day, corresponding to 1.60–1.80 g protein and 14.46–16.27 kcal energy g100 g⁻¹ of the diet/day is optimum for the growth and efficient feed utilization of H. fossilis, while 2–3% ration levels (0.80–1.20 g protein and 7.23–10.84 kcal energy) suggest that these amounts approximate to the maintenance requirement of this fish.  相似文献   

氮肥溶液磁化灌溉下土壤入渗特征和水氮迁移规律研究   总被引：1，自引：0，他引：1  

李佳蓓  张富仓  段晨骁  ABDELGHANY Ahmed Elsaye  杨玲  李志军 《农业机械学报》2022,53(7):316-324

为探明不同浓度氮肥溶液磁化前后土壤入渗特征和水氮迁移规律,采用恒定磁场强度300 mT对质量浓度分别为0、0.4、0.7、1.1 g/L的硝酸钾溶液进行磁化处理,以未磁化处理为对照,测定各处理溶液的电导率、pH值、溶氧量、表面张力、累积入渗量、湿润锋运移距离和入渗后不同土壤剖面水氮迁移分布。试验结果表明：磁化处理溶液溶氧量显著提高,电导率和表面张力显著减小,并随溶液浓度变化有显著影响,但对pH值无显著影响。氮肥溶液磁化入渗增大了相同入渗时间内的湿润锋运移距离和累积入渗量,Philip、Green-Ampt、一维代数入渗模型拟合所得参数土壤吸渗率S、饱和导水率K_s以及有效土壤水扩散率■均增大,湿润峰处的土壤水吸力S_f、土壤水分特征曲线和非饱和导水率综合形状系数m均减小,增渗效果随氮肥溶液浓度增大而增大。磁化氮肥溶液可提高土壤持水能力,且随溶液浓度增大持水能力增强,一维代数入渗公式可较好描述不同磁场强度下各浓度溶液土壤入渗结束时的土壤含水率分布情况。氮肥溶液和磁化作用对土壤硝态氮含量的影响呈显著正相关关系,二者共同作用下,磁化高浓度溶液硝态氮含量...  相似文献