Digestibility of Quinoa (<Emphasis Type="Italic">Chenopodium quinoa</Emphasis> Willd.) Protein Concentrate and Its Potential to Inhibit Lipid Peroxidation in the Zebrafish Larvae Model     

R. Vilcacundo  D. Barrio  C. Carpio  A. García-Ruiz  J. Rúales  B. Hernández-Ledesma  W. Carrillo 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(3):294-300

Quinoa protein concentrate (QPC) was extracted and digested under in vitro gastrointestinal conditions. The protein content of QPC was in the range between 52.40 and 65.01% depending on the assay used. Quinoa proteins were almost completely hydrolyzed by pepsin at pH of 1.2, 2.0, and 3.2. At high pH, only partial hydrolysis was observed. During the duodenal phase, no intact proteins were visible, indicating their susceptibility to the in vitro simulated digestive conditions. Zebrafish larvae model was used to evaluate the in vivo ability of gastrointestinal digests to inhibit lipid peroxidation. Gastric digestion at pH 1.2 showed the highest lipid peroxidation inhibition percentage (75.15%). The lipid peroxidation activity increased after the duodenal phase. The digest obtained at the end of the digestive process showed an inhibition percentage of 82.10%, comparable to that showed when using BHT as positive control (87.13%).  相似文献   

Spatial patterns of the Citrus leprosis virus and its associated mite vector in systems without intervention     

R. B. Bassanezi  A. B. C. Czermainski  F. F. Laranjeira  A. S. Moreira  P. J. Ribeiro Júnior  E. T. Krainski  L. Amorim 《Plant pathology》2019,68(1):85-93

Leprosis is caused by the Citrus leprosis virus cytoplasmic type and is vectored by the mite Brevipalpus yothersi. Miticide applications, which cost $54 million annually, are based on inspection for the presence of mites. The aim of the present study was to characterize the spatial patterns of B. yothersi-infested trees and trees with leprosis symptoms for further improvement in sampling and disease control. The presence of mites and the occurrence of leprosis were assessed over two years in 1160 Valencia trees and 720 Natal trees in a commercial sweet orange grove in Sao Paulo State, Brazil. To assess the natural growth and dispersal of mites and leprosis, mite populations were not controlled during the experimental period. Maps of mite-infested trees and trees with leprosis symptoms were analysed at three different levels of spatial hierarchy using complementary methods, i.e. among adjacent trees within and across rows, within quadrats, and the strength and orientation of aggregation among quadrats. The study showed that the spatial patterns of virus-infected and mite-infested trees were different, with a strong aggregation pattern of trees with leprosis symptoms that increased over time. Conversely, the spatial pattern of B. yothersi showed randomness or weak aggregation at all three spatial hierarchical levels. Disease incidence increased steadily in plots of both cultivars, unlike in mite-infested trees where incidence fluctuated over time. These results have important implications for the development of better management strategies for leprosis. Sampling methods and action thresholds for mite control should consider primary disease inoculum in addition to the incidence of mites.  相似文献   

tau‐Fluvalinate and other pesticide residues in honey bees before overwintering     

Tomas Erban  Marta Vaclavikova  Daniela Tomesova  Tatana Halesova  Jan Hubert 《Pest management science》2019,75(12):3245-3251

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The ecosystem services concept: a new Esperanto to facilitate participatory planning processes?     

Spyra  Marcin  Kleemann  Janina  Cetin  Nuket Ipek  Vázquez Navarrete  Cesar Jesús  Albert  Christian  Palacios-Agundez  Igone  Ametzaga-Arregi  Ibone  La Rosa  Daniele  Rozas-Vásquez  Daniel  Adem Esmail  Blal  Picchi  Paolo  Geneletti  Davide  König  Hannes J.  Koo  HongMi  Kopperoinen  Leena  Fürst  Christine 《Landscape Ecology》2019,34(7):1715-1735

Landscape Ecology - Several case studies investigated the role of ecosystem services in participatory planning processes. However, no systematic study exists that cuts across a large number of...  相似文献   

Direct analysis of pesticide residues in olive oil by on-line reversed phase liquid chromatography-gas chromatography using an automated through oven transfer adsorption desorption (TOTAD) interface     

Sanchez R  Vázquez A  Riquelme D  Villén J 《Journal of agricultural and food chemistry》2003,51(21):6098-6102

A fully automated on-line reversed phase liquid chromatography-gas chromatography system is described. The system uses a prototype of the automated through oven transfer adsorption desorption interface. The system is demonstrated by presenting a new rapid method for the determination of pesticide residue in olive oil, which is injected directly with no sample pretreatment step other than filtration. Methanol:water is used as the eluent in the LC preseparation step, while the LC fraction containing the pesticide is automatically transferred to the gas chromatograph. Detection limits of pesticides varied from 0.18 to 0.44 mg/L when a flame ionization detector was used. As an example, relative standard deviation and linear calibration are presented for terbutryne.  相似文献   

Migration studies to assess the safety in use of a new antioxidant active packaging     

Tovar L  Salafranca J  Sánchez C  Nerín C 《Journal of agricultural and food chemistry》2005,53(13):5270-5275

Both specific and overall migration tests have been applied to new experimental food packaging-active plastic films with antioxidant properties, including in its composition a natural rosemary extract. Determination of volatile and semivolatile compounds migrating from plastic to the four established simulants showed that both specific and overall migration was very low. The results obtained gave values 20 times lower than the established limits in the worst case. So, from the point of view of health risk, the new active packaging can be considered as safe. Analytical procedure used provided the necessary information about the migration behavior, with good analytical characteristics and detection limits in the sub mug kg(-1) range. Besides, no significant difference was found between laboratory and factory-made samples, which is an important issue for industrial production, the next step in the development of the new antioxidant active film.  相似文献   

Potential Anti-Obesity,Anti-Steatosis,and Anti-Inflammatory Properties of Extracts from the Microalgae Chlorella vulgaris and Chlorococcum amblystomatis under Different Growth Conditions     

Ana Regueiras  lvaro Huguet  Tiago Conde  Daniela Couto  Pedro Domingues  Maria Rosrio Domingues  Ana Margarida Costa  Joana Laranjeira da Silva  Vitor Vasconcelos  Ralph Urbatzka 《Marine drugs》2022,20(1)

Microalgae are known as a producer of proteins and lipids, but also of valuable compounds for human health benefits (e.g., polyunsaturated fatty acids (PUFAs); minerals, vitamins, or other compounds). The overall objective of this research was to prospect novel products, such as nutraceuticals from microalgae, for application in human health, particularly for metabolic diseases. Chlorella vulgaris and Chlorococcum amblystomatis were grown autotrophically, and C. vulgaris was additionally grown heterotrophically. Microalgae biomass was extracted using organic solvents (dichloromethane, ethanol, ethanol with ultrasound-assisted extraction). Those extracts were evaluated for their bioactivities, toxicity, and metabolite profile. Some of the extracts reduced the neutral lipid content using the zebrafish larvae fat metabolism assay, reduced lipid accumulation in fatty-acid-overloaded HepG2 liver cells, or decreased the LPS-induced inflammation reaction in RAW264.7 macrophages. Toxicity was not observed in the MTT assay in vitro or by the appearance of lethality or malformations in zebrafish larvae in vivo. Differences in metabolite profiles of microalgae extracts obtained by UPLC-LC-MS/MS and GNPS analyses revealed unique compounds in the active extracts, whose majority did not have a match in mass spectrometry databases and could be potentially novel compounds. In conclusion, microalgae extracts demonstrated anti-obesity, anti-steatosis, and anti-inflammatory activities and could be valuable resources for developing future nutraceuticals. In particular, the ultrasound-assisted ethanolic extract of the heterotrophic C. vulgaris significantly enhanced the anti-obesity activity and demonstrated that the alteration of culture conditions is a valuable approach to increase the production of high-value compounds.  相似文献   

Microbial HemG‐type protoporphyrinogen IX oxidase enzymes for biotechnology applications in plant herbicide tolerance traits     

Clayton T Larue  Joel E Ream  Xuefeng Zhou  Farhard Moshiri  Arlene Howe  Michael Goley  Oscar C Sparks  Steven T Voss  Erin Hall  Christine Ellis  Janice Weihe  Qungang Qi  Daniela Ribeiro  Xiaoping Wei  Shirley Guo  Artem G Evdokimov  Marguerite J Varagona  James K Roberts 《Pest management science》2020,76(3):1031-1038

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Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs     

Robson RV Alves  Tatiana Soares  Elinaldo FL Bento  Ricardo S Roldan‐Filho  Brbara SS Souza  Marcele KN Lima  Jssica S Nascimento  Luana CBB Coelho  Roberto A S  Thmarah A Lima  Gabriel GA Gonalves  Fbio A Brayner  Luiz C Alves  Daniela MAF Navarro  Thiago H Napoleo  Patrícia MG Paiva 《Pest management science》2020,76(2):730-736

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Replacing Wheat Flour with Debittered and Fermented Lupin: Effects on Bread’s Physical and Nutritional Features     

Villacrés  Elena  Cueva  Paúl  Díaz  Milene  Rosell  Cristina M. 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(4):569-575

Plant Foods for Human Nutrition - In this study the breadmaking potential of lupin flour from L. mutabilis after being debittered (DLF) and solid state fermented (FLF) was evaluated in lupin-wheat...  相似文献