High water content silk protein-based hydrogels with tunable elasticity fabricated via a Ru(II) mediated photochemical cross-linking method     

Di Zhang  Han Peng  Baochang Sun  Shanshan Lyu 《Fibers and Polymers》2017,18(10):1831-1840

Silk is very promising in the field of biomaterials as a natural biomacromolecule. Silk protein can be made into various forms of materials, including hydrogels. However, silk protein-based hydrogels have not attracted much attention due to its weak mechanical properties. Here, we report high water content silk protein-based hydrogels with tunable elasticity which were fabricated through Ru(II) mediated photochemically cross-linking tyrosine residues in regenerated silk protein. The regenerated silk protein was characterized by Fourier transform infrared spectroscopy (FTIR). The gelation kinetics of the silk protein was studied by rheology measurements. The compressive mechanical properties of the silk protein-based hydrogels was investigated using compressive tests and dynamic mechanical analysis (DMA). Compressive modulus of the hydrogels reached 349±64 MPa at 15 % strain. The fabricated silk protein-based hydrogels were also characterized by Scanning electron microscopy (SEM), revealing an interconnected porous network structure, typical of hydrogels, with an average pore size of approximately 130 μm. Finally, biocompatibility of the silk protein-based hydrogels was demonstrated through cell culture studies using a human fibroblast cell line, HFL1. The reported silk protein-based hydrogels represent a promising candidate for biomaterial applications.  相似文献   

Pervasive interactions of <Emphasis Type="Italic">Sa</Emphasis> and <Emphasis Type="Italic">Sb</Emphasis> loci cause high pollen sterility and abrupt changes in gene expression during meiosis that could be overcome by double neutral genes in autotetraploid rice     

Jinwen Wu  Lin Chen  Muhammad Qasim Shahid  Minyi Chen  Qinglei Dong  Jirui Li  Xiaosong Xu  Xiangdong Liu 《Rice》2017,10(1):49

  相似文献   

Nano-calcium carbonate reinforced polypropylene and propylene-ethylene copolymer nanocomposites: Tensile vs. impact behavior     

Mangala Joshi  Satyajit Brahma  Anasuya Roy  S. Wazed Ali 《Fibers and Polymers》2017,18(11):2161-2169

Improvement of both the tensile and impact strength of the same polymeric material has always been a great challenge for the plastic industry. The study focuses on the effect of incorporation of calcium carbonate nanoparticles (0.3 wt% to 15 wt%) into three polypropylene (PP) based matrices viz. PP homopolymer, propylene-ethylene (PP-PE) copolymer and the blend of PP:PP-PE (30:70) to improve their impact behavior without hampering the tensile strength much. A loss in both the tensile and impact properties was observed in PP based nanocomposite. However, PP-PE based nanocomposites showed a significant improvement in impact strength (47 %) at 10 wt% loading with a loss of tensile strength by 22 %. To minimize this loss a blend of PP:PP-PE (30:70) was explored as a matrix. At 10 wt% loading, this matrix showed an improvement of 30 % in impact strength whereas the tensile loss was minimized to 10 %. Further, silane coupling agent which promoted good interfacial adhesion was used for best compositions. The variation of crystalline morphology of the nanocomposites with various formulations was analyzed using differential scanning calorimetry and X-ray diffraction.  相似文献   

抗禽流感病毒H5和H9亚型血凝素特异性单克隆抗体的研制及应用   总被引：23，自引：0，他引：23  

秦爱建  邵红霞  钱琨  刘岳龙  金文杰 《中国预防兽医学报》2003,25(3):161-163

以禽流感题H5和H9亚型病毒分别免疫Balh／c小鼠，取其脾细胞与SP2／0的骨髓瘤细胞融合，用血凝抑制试验(HI）检测细胞培养上清，结果获得了6株特异性单克隆抗体，其中抗禽流感题亚型病毒血凝素特异性单克隆抗体细胞株3株，分别命名为4B6、4A3、3H1；抗H9亚型病毒血凝素单克隆抗体细胞株3株，命名为6E6、6B6和5B4。这些单克隆抗体小鼠腹水HI效价为2^13-15，细胞培养上清抗体HI效价为2^7-8。研究结果表明，所有这些单克隆抗体仅与试验的相应题或ID亚型病毒株发生特异性反应，而不能与鸡新城疫病毒、鹅新城疫病毒、鹅腺病毒和鸡产蛋下降综禽征病毒(EDS76)等反应。实验室检测结果证明，应用这些单克隆抗体能在24h内迅速检测出相应的禽流感病毒。所有这些单克隆抗体将在禽流感的预警预报工作中发挥重要作用。  相似文献   

鹿角炭角菌等三种真菌多糖对HIV-RT的作用   总被引：2，自引：0，他引：2  

刘庆洪  罗倩怡  冯昆  王贺祥 《中国食用菌》2004,23(4):29-30

热水抽提和酒精沉淀的方法提取鹿角炭角菌、香栓菌、茶树菇的多糖复合物 ,在反应浓度为 1mg/mL时 ,三种多糖对HIV逆转录酶活性的抑制率分别为 (80 4± 1 2 ) %、 (90 6± 1 7) %和 (84 5± 2 1) %。  相似文献   

聚乙烯吡咯烷酮修饰碳糊电极法测定水中苯酚     

王凯雄  徐娟宝  臧荣春  彭图治  王国顺 《浙江大学学报(农业与生命科学版)》1996,(3)

提出了以聚乙烯吡咯烷酮（ＰＶＰｒ）修饰碳糊电极测定水中苯酚的方法．在０．１ｍｏｌ／ＬＫＣｌ的酚试液中开路富集后，在磷酸盐缓冲液介质（ｐＨ５．６）中溶出，用微分脉冲伏安法测定，苯酚的溶出峰电位为＋０．７３Ｖ（Ａｇ－ＡｇＣｌ参比电极），检测限为０．０５μｇ／Ｌ．应用该法测定了废水中苯酚的含量，并讨论了该法测定苯酚的影响因素，比较了几种修饰电极测定苯酚的灵敏度，探讨了苯酚在该修饰电极上的富集机理  相似文献   

虎皮黄兔主要肉用性能评定及其估（预）测   总被引：2，自引：0，他引：2  

臧素敏  张宝庆 《河北农业大学学报》1994,17(4):72-76

以３５只虎皮黄免为材料，对其增重、屠宰率及肉品品质等性状进行评定，利用通径分析法研究主要向用性状间的相关关系及其估（预）测．结果表明，１～３月龄平均日增重为２２．５９ｇ；３月龄宰前活重为１８９５ｇ；屠宰率为５２％；背最长肌水分含量为７５％，ｐＨ２为５．７，脂肪含量为２．０１％，失水率为１５．９％．用体长、胸围估测体重，用体重、体长预测肌间脂肪含量和颜色深浅，用胸围和耳长预测失水率可获得较可靠的结果。  相似文献   

二氢杨梅素在动物营养中的应用     

张丽  臧素敏 《饲料工业》2006,27(18):16-17

藤茶是显齿蛇葡萄的嫩叶经过杀青、揉捻、烘干而成的茶饮料。显齿蛇葡萄(Ampelopsis grossedentata)是葡萄科蛇葡萄属的木质藤本植物,具有极高的营养、保健、医疗价值。现代医学药理研究和临床上都已证明,显齿蛇葡萄全株药用,其味甘淡、性凉,具有清热解毒、抗菌消炎、祛风湿、降血糖等功效。藤茶由多糖、氨基酸、蛋白质、多肽、甾类、萜类、有机酸、挥发油、油脂、生物碱、二氢杨梅素以及多种酶和无机离子等组成,其中二氢杨梅素(DMY)是藤茶的主要活性成分之一,具有多种生物学功能。DMY又名双氢杨梅树皮素、福建茶素、白蔽素、二氢杨梅黄…  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

葡萄自交与杂交后代香气成分的遗传研究     

李坤  郭修武  谢洪刚  郭印山  李轶辉  李成祥 《园艺学报》2005,32(2):218-221

 利用气相色谱- 质谱- 计算机联用系统(GC-MC-CS) 对两个自交组合、1个杂交组合亲本及其后代的香气成分进行研究。结果表明: 同一组合中不同香气成分的分离情况不同, 同种香气成分在不同组合中的分离情况也不完全相同。果实香气成分的遗传主要表现为分离( 1∶1、1∶3、3∶1、15∶1) 与不分离两种方式, 发生分离的成分有些是由单基因控制的, 有些是由多基因控制的。  相似文献