表达猪生长激素基因的重组腺病毒的构建及对猪生长的促进效果观察   总被引：1，自引：0，他引：1  

曹汉威  张春红  黄忠  阮良基  林洁 《畜牧兽医学报》2006,37(6):592-596

利用复制缺陷型重组腺病毒作为基因表达载体,将猪生长激素（pGH）基因直接转导到猪体内细胞,研究猪生长激素基因在猪体内的促生长作用.用同源重组的方法,构建含pGH基因的重组腺病毒（Ad-pGH）.将其感染293细胞和PK-15细胞,均可检测到pGH.用1 mL此重组腺病毒（1010TCID50）一次性肌肉注射60日龄的杂交长白猪.结果表明,试验组的猪平均增重比对照组的猪增加37%（注射后第4周）和19%（注射后第6周）;饲料报酬提高28%（注射后第4周）和18%（注射后第6周）.试验结果证明,由重组腺病毒表达的pGH具有生物学活性,在猪体内起到明显的促进生长的作用.重组腺病毒介导的pGH基因在猪体内的表达可维持约4周.  相似文献   

纳米技术在生物医学中的研究进展     

吴艳花阮文科  彭丽英 《动物医学进展》2007,28(B08):64-65

20世纪80年代开始研究的纳米技术在90年代获得了突破性进展，它给许多行业带来巨大变化，它对生物医学的渗透与影响是显而易见的。利用纳米技术可将生物降解性和生物相容性的聚合物与药物一起制成纳米药物，作为靶向药物制剂，直接导入病灶部位的器官、组织甚至细胞，达到提高药物疗效，降低毒性的作用；将纳米材料作为药物载体，可增加某些药物的胃肠吸收，提高其生物利用度；将纳米材料作为载体，可用于基因的输送和治疗。文章就纳米技术在生物医学中的研究进展做一综述。  相似文献   

猪臭鼻克雷伯氏菌的分离与鉴定   总被引：2，自引：0，他引：2  

王茂法  尤康春  林平  郑伟  阮文琴 《中国兽药杂志》2006,40(2):39-40

从呼吸系统症状明显、高热、耳朵等薄皮处发绀的病猪血与病死猪的心血、肝、脾中分离到1株细菌，经形态学观察、生化试验鉴定为臭鼻克雷伯氏菌；经人工感染小白鼠试验，表明具有较强的毒性。选用药敏试验筛选的敏感药物——头孢曲松钠治愈了病猪，控制了疫情。  相似文献   

应用可拓学原理评价黄瓜根分泌物的化感潜势     

左胜鹏  税军峰  马永清  黄晓珊 《园艺学报》2006,33(2):399-401

 对黄瓜组培根分泌物的化感潜势进行可拓评价, 得出黄瓜组培根分泌物对菜豆幼苗生长有显著促进作用, 而对其它3种作物有明显的毒害作用, 毒害程度为黄瓜>甜瓜>番茄。同时, 建立了黄瓜组培根分泌液的化感效应的数学模型R_E = [ k₁R_p0 / ( k₂ - k₁ ) ] ( e^{- k1t} - e^{- k2t} ) ( e- ^k1a - e^{- k2a} ) , 说明了黄瓜组培根分泌液的化感效应与组培根培养时间( t) 以及根分泌液浓度( a) 显著相关。黄瓜根分泌物对4种受体作物显示出显著的化学干扰差异, 论证了植物种内(间) 发生的干扰作用与植物的品种(系) 有关, 从理论上得出菜豆可以作为黄瓜的间套作物或下茬作物。  相似文献   

Role of neutrophil elastase and myeloperoxidase in asthma of rats     

TONG Xia-sheng  LI Chang-chong  RUAN Zheng-ying  CHEN Hao  GUO Hai-yuan  FAN Guang-min 《园艺学报》2006,22(7):1397-1401

AIM:To observe the changes of polymorphonuclear leukocytes (PMN), neutrophil elastase (NE) and myeloperoxidase (MPO) expression in rat asthma. METHODS:Eighteen rats were randomly divided into two groups on average, including asthma group and control group. The rat model of asthma was established by the ovalbumin (OVA) challenge methods. Blood PMN were isolated andpurified. The protein expression of MPO were detected by immunohistochemistry and chromatometry techniques. NE was detected by ELISA. RESULTS:(1) Immunohistochemistry showed that the levels of MPO expression around bronchus and in purified PMN in asthma group were significantly increased compared with those in control group (P<0.01). Activities of MPO were significantly increased in bronchoalveolar lavage fluid (BALF) and lung tissue in asthma group compared with those in control group (P<0.05, P<0.01). (2) Levels of NE were significantly increased in BALF and PMN in asthma group compared with control group (P<0.01). (3) Number of PMN were significantly higher in BALF, bronchus and lung tissue in asthma group than those in control group (P<0.01). CONCLUSION:Levels of PMN counting and expression of MPO and NEare significantly increased in experimental asthma rats. PMN may be involved in inflammation in asthma via increasing the levels of NE and MPO.  相似文献   

不同果袋类型对紫花杧的套袋效果     

欧世金  卢美英  韦巍  卢志伟  何达标  梁北森  阮瑛 《福建果树》2007,(4):1-4

以主栽品种紫花杧为试材,探讨不同果袋类型的套袋效果.结果表明:套袋可大幅度降低采收果的果面流胶和雨水污染,极显著地降低果面虫伤率.套袋可改变采收时的果面颜色,但不同果袋类型间存在差异.后熟完成时,果面均为品种固有颜色,不因果袋种类而改变.套袋能促进果实后熟转黄和果色均匀,能显著降低炭疽病的病果率,但不同果袋对蒂腐病的防效存在较大差异.套袋能提高可溶性固形物含量和减轻果实失重.供试品种用外黄内黑复合纸袋套袋效果最为理想.  相似文献   

Etoposide induces apoptosis via mitochondrial signaling pathway with cytochrome c release in Jurkat leukemia cells     

LIU Jia-hao  TANG Hong-li  RUAN Wei-yong 《园艺学报》2007,23(3):453-459

AIM:To investigate the molecular mechanisms of apoptosis and to elucidate the apoptosis signaling pathway triggered by etoposide in Jurkat human leukemia cells. METHODS:Apoptosis was detected using annexin V-FITC and propidium iodide (PI) staining, respectively, and annexin V-FITC positive cells and hypodiploid cells were analyzed by flow cytometry. Mitochondrial membrane potential (△Ψm) was detected using 3, 3-dihexyloxycarbocyanine iodide ［DiOC6(3)］ staining and △Ψm low cells were analyzed by flow cytometry. Preparation of cytosolic extracts and isolation of mitochondria were completed by centrifugation. Western blotting analysis was used to evaluate the level of cytochrome c, caspase-3, and poly (ADP-ribose) polymerase (PARP) expression. RESULTS:Etoposide induced apoptosis showing phosphatidylserine externalization and DNA fragmentation in a time-dependent manner and the apoptosis could be inhibited by a broad caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (zVAD.fmk). Collapse of △Ψm induced by etoposide preceded DNA fragmentation and phosphatidylserine externalization. In contrast, it was not blocked by zVAD.fmk. Etoposide caused cytochrome c release from mitochondria into cytosol, subsequent activation of caspase-3 (32 kD) presented with an intermediate (20 kD) and its active product (17 kD), and cleavage of full-length PARP (116 kD) into the so-called apoptotic 85 kD fragment. CONCLUSION:Etoposide-induced Jurkat cell apoptosis is initiated through mitochondria signaling pathway with cytochrome c release into cytoplasm and caspase is the ultimate executioner of cell apoptosis.  相似文献   

抗香蕉枯萎病菌拮抗菌的鉴定及其定殖   总被引：4，自引：0，他引：4  

杨秀娟  何玉仙  陈福如  阮宏椿 《中国生物防治》2007,23(1):73-77

通过逐步提高药物浓度方法,获得了抗利福平400μg/ml、对香蕉枯萎病菌拮抗活性稳定的T2WF和W10标记菌株。初步确定两菌株为芽孢杆菌属细菌。采用灌根接种法,在香蕉苗根际土和香蕉苗体内定殖结果表明,拮抗菌液接种浓度为5×104cfu/ml,两菌株均可从香蕉根际土和香蕉体内分离到,并在香蕉体内定殖和传导。在香蕉根际土、根部、球茎和假茎中,T2WF标记菌的菌量高峰分别出现在接种后5、5、11和3d,分别为707、437、273、117cfu/mg;而W10标记菌的菌量高峰分别出现在接种后5、3、5和5d,分别为784、260、253、110cfu/mg。两菌株在香蕉根际土和香蕉体内1~15d的消长动态均有一个共同的“由升到降”趋势。从数量和数量变动幅度上看,香蕉根部的菌量明显高于茎部的菌量。  相似文献   

Li~+、肌醇与Ca~(2+)对苹果、梨、山楂花粉萌发和花粉管生长的影响     

关军锋 《果树学报》1998,(4)

观测了苹果（金冠）、梨（鸭梨）、山植（面值）的花粉萌发和花粉管生长情况,结果表明,Li2SO4的浓度越高、处理时间越早,花粉萌发和花粉管生长的抑制程度越大,Ca2+和肌醇可不同程度地逆转上述效应。因此认为,细胞内一定量的肌醇和Ca2+是果树花粉萌发和花粉管生长所必需的,并对维持正常的肌醇磷脂代谢是至关重要的。  相似文献   

关于推进西双版纳天然橡胶初加工行业标准化管理的对策分析     

李燕飞  阮林光  张伟  杨馗 《热带农业科技》2013,(1):19-21,42

分析了西双版纳天然橡胶制胶行业现状和行业标准化管理水平滞后的原因,提出了加快制胶行业标准化管理进程意见和措施。  相似文献