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61.
Naoki SASAKI Nao TSUZUKI Michiaki YAMADA Takuto MINAMI Haruo YAMADA 《Journal of Equine Science》2009,20(1):7-10
This study aimed to establish a standard dose and sample collection time for
13C phenylalanine and 13C-Dipeptide breath test in horses. To
evaluate dose-dependent effects, healthy horses received 2.5 mg/kg, 5 mg/kg, and 10 mg/kg
13C phenylalanine dissolved in 1 ml/kg distilled water and 1.25 mg/kg, 2.5
mg/kg, and 5 mg/kg 13C dipeptide dissolved in 2 ml/ kg distilled water. Tmax
was observed during the sample collection time. For 13C phenylalanine, the
standard deviation of Cmax at 5 mg/kg was lower than that of 10 mg/kg. For 13C
dipeptide, the standard deviation of Tmax was the lowest at 5 mg/kg. This study revealed
that an optimal dose for breath tests with 13C phenylalanine and 13C
dipeptide may be 5 mg/kg in horses. 相似文献
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Kensuke HIROSE Mariko NAKAMURA Tatsuya TAKIZAWA Kazuo FUKAWA Tetsuya ITO Masayuki UEDA Takashi SASAKI Kazuaki TANAKA 《Animal Science Journal》2009,80(6):624-630
An insertion/deletion variant of a thymine base (T5 and T6) in exon 2 of porcine beta 3‐adrenergic receptor (ADRB3) gene has been described. In the current study, we made an association study between the ADRB3 polymorphisms and production traits in 735 Duroc pigs. The allele frequencies for the T5 and T6 alleles in our study population were 0.433 and 0.567, respectively. Any associations between ADRB3 genotype and average daily weight gain during test period, or backfat thickness and intramuscular fat content were not detected in either sex. However the size of the loin eye muscle area (EMA) was significantly associated with ADRB3 genotypes in gilts. T6‐homozygous gilts had a higher mean of EMA (40.6 ± 0.6 cm2) than T5‐homozygous (38.1 ± 0.4 cm2, P = 0.002) and heterozygous (38.8 ± 0.3 cm2, P = 0.034) gilts. This association was not detected in males. In addition, a multiple traits animal model best linear unbiased predictor (BLUP) analysis revealed that the T6‐homozygous genotype had positive effects on breeding value of EMA. Accordingly, we suggest that ADRB3 polymorphism has the potential to be an important genetic marker for prediction of EMA in Duroc pigs. 相似文献
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Hiroshi OHTA Noriyuki NAGATA Nozomu YOKOYAMA Tatsuyuki OSUGA Noboru SASAKI Keitaro MORISHITA Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):378
To date, little is known about the prognostic significance of ultrasonographic findings in dogs with protein-losing enteropathy (PLE). The aim of this retrospective study was to examine the prognostic value of ultrasonographic findings in dogs with PLE. A total of 26 dogs with PLE were included: 20 dogs with chronic enteropathy and 6 dogs with gastrointestinal lymphoma. The presence of small intestinal dilatation was associated with shorter survival time in dogs with PLE (P=0.003). The presence of hyperechoic intestinal mucosal striations was associated with longer survival time in dogs with PLE (P=0.0085). The results of the current study indicate that the presence of small intestinal dilatation might be associated with poor prognosis in dogs with PLE. 相似文献
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利用免疫组织化学和形态计测学的方法 ,观察了 1 8匹成熟雌性蒙古马的脑垂体前叶生长激素细胞和催乳激素细胞的数量和面积 ,同时利用放射免疫分析方法检测了这两种激素的血浆水平。结果表明 ,每个马脑垂体前叶中 ,生长激素细胞的平均数量为 6 .42× 1 8,每个细胞的平均面积为 82 .40μm2 ;催乳激素细胞的平均数量为 6 .0 7× 1 0 8,每个细胞的平均面积为 47.31μm2 。生长激素的血浆含量平均为 2 .84ng/ m L,但个体差异较大 ,变异系数高达 78.5 % ,催乳激素的血浆含量平均为 7.2 6 ng/ m L。本研究结果揭示 :母马脑垂体生长激素细胞和催乳激素细胞的数量和面积并不是决定母马这两种激素血中浓度的唯一重要因素 ;生长激素血中浓度上的个体差异 ,可能与其搏动性分泌形式有关 相似文献
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Osamu SASAKI Hirohisa KIMURA Kazuo ISHII Masahiro SATOH Yoshitaka NAGAMINE Kunio YOKOUCHI 《Animal Science Journal》2011,82(3):486-493
Dairy farm agricultural income should increase when sexed semen is used instead of unsexed semen. This study simulated three scenarios: using unsexed semen (N), using X sperm of Holstein and unsexed semen of Japanese Black (H), and using X sperm of Holstein and Y sperm of Japanese Black (HJ). Two management scenarios were considered: conventional management (CONV); and the use of Japanese Black semen with surplus cows to produce F1s where sufficient replacement female Holstein calves were obtained for maintenance (MAINT). In CONV, the agricultural incomes of H and HJ were 610 000–2 400 000 yen higher than that of N. The agricultural income increased when the difference in the prices between Holstein males and average F1 male/female calves was 34 000–50 000 yen, that between Holstein male and female calves was 80 000–110 000 yen, and that between F1 male and female calves was 50 000–90 000 yen. Therefore, the agricultural income can be increased by using sexed semen to select calves of a more valued breed and sex. 相似文献
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Motoki SASAKI Yoko AMANO Daisuke HAYAKAWA Toshio TSUBOTA Hajime ISHIKAWA Toshihiro MOGOE Seiji OHSUMI Masafumi TETSUKA Akio MIYAMOTO Yutaka FUKUI Teguh BUDIPITOJO Nobuo KITAMURA 《The Journal of reproduction and development》2013,59(2):159-167
There are few reports describing the structure and function of the whale placenta with
the advance of pregnancy. In this study, therefore, the placenta and nonpregnant uterus of
the Antarctic minke whale were observed morphologically and immunohistochemically.
Placentas and nonpregnant uteri were collected from the 15th, 16th and 18th Japanese Whale
Research Programme with Special Permit in the Antarctic (JARPA) and 1st JARPA II organized
by the Institute of Cetacean Research in Tokyo, Japan. In the macro- and microscopic
observations, the placenta of the Antarctic minke whale was a diffuse and epitheliochorial
placenta. The chorion was interdigitated to the endometrium by primary, secondary and
tertiary villi, which contained no specialized trophoblast cells such as binucleate cells,
and the interdigitation became complicated with the progress of gestation. Furthermore,
fetal and maternal blood vessels indented deeply into the trophoblast cells and
endometrial epithelium respectively with fetal growth. The minke whale placenta showed a
fold-like shape as opposed to a finger-like shape. In both nonpregnant and pregnant uteri,
many uterine glands were distributed. The uterine glands in the superficial layer of the
pregnant endometrium had a wide lumen and large epithelial cells as compared with those in
the deep layer. On the other hand, in the nonpregnant endometrium, the uterine glands had
a narrower lumen and smaller epithelial cells than in the pregnant endometrium. In
immunohistochemical detection, immunoreactivity for P450scc was detected in most
trophoblast cells, but not in nonpregnant uteri, suggesting that trophoblast epithelial
cells synthesized and secreted the sex steroid hormones and/or their precursors to
maintain the pregnancy in the Antarctic minke whale. 相似文献