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11.
12.
This study was conducted to identify and analyse the expression of gametogenesis‐associated genes and proteins in foetal and adult buffalo gonads of both the sexes. Relative quantification of the genes was determined by qPCR and Western blotting. Immunohistochemistry was also performed for various gametogenesis‐associated proteins in foetal and adult gonads of both the sexes. We observed significantly (p < 0.05) increased expression of primordial germ cell‐specific, meiotic as well as genes associated with oocyte maturation and development in foetal ovaries as compared to the adult ones. However, significantly (p < 0.05) increased expression of proteins associated with oocyte maturation like GDF9 and ZP4 was found in adult ovaries, indicating temporal regulation of mRNA translation during oogenesis. Meiotic genes showed significantly (p < 0.05) increased expression in adult testes as compared to foetal testes and ovaries, indicating onset of meiosis at a later stage in spermatogenesis. In general, the expression of primordial germ cell‐associated as well as meiotic genes was higher in adult testes, indicating the increased biological activity in the organ. Immunohistochemistry revealed localized expression of gametogenesis‐associated proteins in ovarian follicles and seminiferous tubules of testes, while the surrounding somatic tissues were devoid of these proteins. The study gives an understanding of the sequential and temporal events of gene expression as well as mRNA translation during male and female gametogenesis. It could also be concluded that follicles and seminiferous tubules are the functional units of the female and male gonads, respectively, and their function could be enhanced by appropriate chemical and genetic intervention of the somatic tissue immediately surrounding them. This assumes importance in the context that buffalo attains sexual maturity at an older age of 2–3 years and have smaller ovaries with lesser number of primordial follicles in comparison with cattle, which is suggested to be the main reason of their poor breeding performance.  相似文献   
13.
Heterotrophic and autotrophic nitrification in two acid pasture soils   总被引:1,自引:0,他引:1  
Laboratory incubation experiments, using 15N-labeling techniques and simple analytical models, were conducted to measure heterotrophic and autotrophic nitrification rates in two acid soils (pH 4.8-5.3; 1/5 in H2O) with high organic carbon contents (6.2-6.8% in top 5 cm soil). The soils were from pastures located near Maindample and Ruffy in the Northeast Victoria, Australia. Gross rates of N mineralization, nitrification and immobilization were measured. The gross rates of autotrophic nitrification were 0.157 and 0.119 μg N g−1 h−1 and heterotrophic nitrification rates were 0.036 and 0.009 μg N g−1 h−1 for the Maindample and Ruffy soils, respectively. Heterotrophic nitrification accounted for 19% and 7% of the total nitrification in the Maindample and Ruffy soils, respectively. The heterotrophic nitrifiers used organic N compounds and no as the substrate for nitrification.  相似文献   
14.
Antibiotic resistance and ESBL constitute a risk to human and animal health. Birds residing close to humans could mirror the spectrum of human associated antibiotic resistance. Household pigeons were screened in Bangladesh to shed light on human associated, as well as, environmental antibiotic resistance. Escherichia coli from pigeons (n = 150) were tested against 11 antibiotics. 89% E. coli isolates were resistant to one or more critically important human antibiotics like ampicillin, cefadroxil, mecillinam, ciprofloxacin, gentamicin and tigecycline. No carbapenamase-producers were detected and the lower ESBL prevalence (5%) in pigeons. ESBL-producing E. coli isolates had blaCTX-M-15 genes. Pigeons shared some bacterial clones and had bird associated sequence types like E. coli ST1408. Fecal carriage of bacteria resistance of critically important human antibiotics, together with examples of shared genotypes among pigeons, indicate the human-birds and bird to bird transmissions are important in the epidemiology of antibiotic resistance.  相似文献   
15.
An experiment was conducted for 60 d in a recirculatory system consisting of 20 glass aquariums (each of size 41 × 41 × 46 cm) to evaluate meat and bone meal (MBM) as partial replacement for fish meal (FM) in Macrobrachium rosenbergii postlarvae (PLs) with a view to develop an inexpensive nursery diet. Three nursery diets were formulated to contain 32% protein and to be as isoenergetic as possible. FM in these diets was progressively replaced with MBM (Diet 1 contained 25% FM and 14% MBM, Diet 2 contained 20% FM and 20% MBM, and Diet 3 contained 15% FM and 26% MBM). Inclusion levels of mustard oilcake and sesame meal in diets were kept fixed at 10%. A commercial shrimp nursery diet (30% protein) from Saudi Bangla Fish Feed Ltd., Mymensingh, was used as the reference diet (Diet 4). Each treatment had three replicates. PLs of M. rosenbergii (26 ± 0.02 mg) were stocked at the rate of 60 PLs (1 PL/L) in each aquarium. At the beginning, PLs were fed three times daily at a rate of 20% of the body weight and it was gradually reduced to 10% at Day 30. The ranges of water quality parameters in the system were as follows: temperature, 27–29 C; dissolved oxygen, 6.5–8.4 mg/L; pH, 6.8–8.3; and total ammonia, 0.01–0.15 mg/L. The result of the study showed that there was no significant difference (P > 0.05) between the weight gains of PLs fed Diet 1 (containing 14% MBM) and Diet 4 (reference diet) but was significantly higher than other dietary groups containing higher levels of MBM. Diets 1 and 4 showed significantly lower (P < 0.05) apparent food conversion ratios (1.74 and 1.73, respectively). The survival (%) of PLs ranged between 62 and 76% with PLs fed Diets 1 and 4 showing significantly higher (P < 0.05) survival. The result of the study demonstrated that the MBM could be included at 14% level in a nursery diet, and a diet containing 25% FM, 14% MBM, 10% sesame meal, and 10% mustard oilcake may be recommended for profitable production of PLs of M. rosenbergii.  相似文献   
16.
The effects of hybridization of glass fibre on oil palm empty fruit bunch (EFB) and recycled polypropylene-based composites are described in this paper. The compounding process involved extrusion followed by injection moulding technique to prepare the samples for characterizations. Fibre loading were considered as 40 % of the total weight of the blends and EFB:glass fibre ratio was maintained as 30:70, 50:50, 70:30 and 90:10. Two types of coupling agents of maleic anhydride-grafted polypropylene such as polybond-3200 and fusabond P-613 of different molecular weight and maleic anhydride level were used to improve the interfacial adhesion between the fibres and the matrix. Composites were characterized by density, melt flow index, tensile, Izod impact and flexural testing. Morphological images of the fractured surfaces of the composites were examined by field-emission scanning electron microscopy. Samples were also characterized by thermal tests such as thermogravimetric analysis and differential scanning calorimetry to evaluate the thermal and crystalline properties, respectively. Optimization of hybridization of the fibres and effect of coupling agents were evaluated in terms of various properties of the samples. The composite prepared with EFB:glass fibre ratio of 70:30 showed better reinforcing properties than that of others.  相似文献   
17.
Suspensions of mixed rumen bacteria (B), protozoa (P), and mixed rumen microorganisms (BP) prepared from rumen contents of fistulated goats were anaerobically incubated with 1 mM p‐hydroxyphenylacetic acid (HPA) at 39°C for 24 h. Tyrosine (Tyr), phenylalanine (Phe), tryptophan (Trp) and other related compounds in both supernatants and hydrolyzates of microbial cells in all incubations were analyzed by HPLC. Large amounts of Tyr (32.1, 42.7 and 36.1% of disappeared HPA in B, P and BP, respectively) were produced from HPA during a 12 h incubation period. The formation of Tyr in P (178.6 µmol/g MN) was 1.5 and 2 times higher than in B and BP, respectively. Phe (7–11% of the disappeared HPA) and Trp (3–6% of the disappeared HPA) were also synthesized from HPA in B, P, and BP. Phe synthesis in P (46.3 µmol/g MN) was 1.7 times higher than in B but, in contrast, Trp synthesis in B, was 1.6 times higher than in P. The metabolites p‐hydroxyphenylpyruvic acid (in the range of 5–14% of disappeared HPA), phenylacetic acid (1–11%), p‐hydroxybenzoic acid (3–7%) and benzoic acid (1–6%) were produced from HPA in B, P and BP. Phenylpropionic acid (6% of the disappeared HPA) was produced only in B and BP.  相似文献   
18.
Coastal land use across the globe has experienced remarkable rapid change over the recent decades because of extraordinary anthropogenic pressure and climate variability and change. Therefore, quantitative information about coastal land use change is imperative for effective management and planning resources for sustainable development. We analysed the quantitative land use and land cover changes during 1989–2000–2010 periods in three important agroecological zones of the most vulnerable coastal region of Bangladesh using Landsat images (Thematic Mapper/Enhanced Thematic Mapper Plus). In the Ganges Tidal Floodplain, the area under shrimp cultivation greatly increased at the rate of 2·05% per annum. The majority of the shrimp area gained from conversion of single cropland. In the Meghna Estuarine Floodplain, decreased mudflat and water bodies were observed, which was predominantly converted into cropland. In Chittagong Coastal Plain, salt pan–shrimp area increased with the expense of single and/or double cropland. In all the study areas, settlement area considerably increased over time. The dynamics of land use change have been attributed to low and unstable food production in the coastal region. The approach adopted in study and the results obtained from the study would likely be useful for policy making and identifying direction for future studies on the coastal land use in Bangladesh. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   
19.
Pollen tube growth in crosses between Porteresia coarctata and Oryza sativa   总被引:1,自引:0,他引:1  
Summary Comparative study with fluorescence microscopy revealed that the pollen tubes of the self-pollinated rice variety BR-9 reached the ovary within 75 min after pollination. In P. coarctata it took 150 min. In the cross between P. coarctata and BR-9, pollen tubes reached the ovary in 165 min but seed setting was not observed. In the cross between BR-9 and P. coarctata, small and deformed pollen tubes were formed and they failed to grow through the stylodium.  相似文献   
20.
Present status of genetics of rust resistance in flax   总被引:5,自引:0,他引:5  
Summary Present knowledge of host genes conferring resistance to rust in flax and their genetics are reviewed. There are at least 34 genes conferring resistance to rust occurring in seven groups, namely, K, L, M, N, P, D and Q. Expression of these host genes is affected by temperature, genetic background and by the inhibitor gene present in certain rust strains. Recombination analysis indicates that genes within each of the M and N groups are probably closely linked and that of the L group are genetically complex. When testcross progeny between two genes of the L group were screened, susceptible and modified recombinants were recovered. Some of these susceptible recombinants yielded rare resistant revertants in their progeny. Mechanism of such reversion is not defined but appears to follow a definite pattern. It is also indicated that some of the recombinants represent new specificity. A molecular approach of cloning host genes in flax is described.  相似文献   
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