Unusual case of osteopenia associated with nutritional calcium and vitamin D deficiency in an adult dog     

de Fornel-Thibaud P  Blanchard G  Escoffier-Chateau L  Segond S  Guetta F  Begon D  Delisle F  Rosenberg D 《Journal of the American Animal Hospital Association》2007,43(1):52-60

A 6-year-old, spayed female rottweiler was presented for facial enlargement from swelling of the maxilla and mandible. The dog was fed a homemade diet deficient in calcium and vitamin D, suggesting that rubber jaw syndrome was a secondary nutritional disorder. Radiographic and tomodensitometric examinations revealed diffuse bone resorption in the skull. The plasma parathormone concentration was high, and serum 25-hydroxycholecalciferol concentration was low. Based on these findings, nutritional calcium and vitamin D deficiency associated with secondary hyperparathyroidism was diagnosed. Dietary correction resulted in clinical and biological improvement, with an increase in skull mineralization.  相似文献   

Comparison of three ultrasound guided approaches to the lumbar plexus in dogs: a cadaveric study          下载免费PDF全文

Sophie M Graff  Deborah V Wilson  Laurent P Guiot  Nathan C Nelson 《Veterinary anaesthesia and analgesia》2015,42(4):394-404

ObjectiveTo assess the accuracy of contrast material injection and the dispersion of injectate following ultrasound guided injections at the level of L6 and L7, in canine cadavers.Study designProspective, randomized, experimental study.AnimalsTwenty nine mixed breed canine cadavers (28.9 ± 6.0 kg).MethodsThree ultrasound-guided approaches to the lumbar plexus (LP) were compared: 1) a dorsal pre-iliac approach at the level of L6; 2) a lateral paravertebral approach at mid-L6; and 3) a lateral paravertebral approach at mid-L7. An isovolumic mixture of iodine-based contrast with new methylene blue (0.1 mL kg⁻¹) was injected bilaterally in the juxta-foraminal region along the L6 or L7 nerve root. Computed tomography was performed followed by segmentation and 3D reconstruction of the lumbar spine and contrast material volumes using dedicated software. Distances between contrast material and the fifth through seventh lumbar foraminae, and length of femoral (FN) and obturator (ON) nerve staining were measured and compared between approaches (p < 0.05).ResultsInjectate moved cranial and caudal to the site of injection, and dispersed into an ovoid shape between the quadratus lumborum, iliopsoas and psoas minor muscles. Injections at L7 resulted in significantly closer contrast proximity to the L6 and L7 foraminae (p < 0.001). Femoral nerve staining was similar for all approaches, ON staining was more consistent after L7 injections (p < 0.001).Conclusion and clinical relevanceAn ultrasound-guided lateral paravertebral approach to the LP proved very practical and accurate, with easy visualization of the plexus and associated nerves. To ensure that the ON is covered by injectate, an approach at the level of L7 is recommended. Further studies are necessary to determine if this correlates with clinically effective local anesthesia.  相似文献   

Biodegradation of estrone and 17 β-estradiol in grassland soils amended with animal wastes     

Sophie D. Lucas 《Soil biology & biochemistry》2006,38(9):2803-2815

The release of endocrine disrupting chemicals into the environment is of increasing concern due to the formation of an intersex state in freshwater organisms and potential risks to human health. The aim of this study was to investigate the persistence of the naturally occurring hormones, estrone and 17 β-estradiol in three agricultural grassland soils in the presence and absence of cattle and sheep wastes (urine and manure). Biodegradation was investigated using ¹⁴C-labelled hormones which were applied to soil in three different solvents (water, artificial urine and natural sheep urine). When applied directly to soil the two hormones degraded at a similar rate, however, the speed of mineralization was soil type and solvent dependant. The half-life (t_1/2) of the hormones in soils ranged from 5 to 25 d. The hormones were also applied to the soils in sheep and cattle manure of different ages (7 d to 2 yr). Generally, the rate of degradation in the animal manure amended soils was more rapid than in the unamended soils (t_1/2=1-9 d), with mineralization being largely independent of manure age and type. We conclude that in comparison to many xenobiotics, estrogens are not persistent in agricultural soils. However, our calculations suggest that if they are lost to freshwater via runoff or leaching then they may have an appreciable effect on freshwater organisms. Assuming normal landspreading rates our results suggest that the risk of estrogen contamination of freshwater associated with manure spreading is very low.  相似文献   

Biological control of damping-off caused byPythium ultimum andRhizoctonia solani usingTrichoderma spp. applied as industrial film coatings on seeds     

Sophie Cliquet  Rudy J. Scheffer 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(3):247-255

Conidia of sevenTrichoderma strains were applied on cucumber or radish seeds as a simple methyl cellulose coating or through an industrial film coating process. The seeds were sown in a peat-based soil artificially infested byR. solani orP. ultimum. Four strains controlled damping-off caused byR. solani when applied as a simple coating or as an industrial film-coating. Also, four strains significantly reduced damping-off caused byP. ultimum in cucumber. A correlation was found between production of volatile antibiotics in vitro and control ofP. ultimum. Survival during storage varied according to the strain. Better survival was observed for two strains, with a decrease in conidial viability of one order of magnitude after storage for three and five months at 15 ° C and 4 ° C, respectively. The results show the feasibility of biocontrol of seedling diseases by some antagonists applied onto seeds through an industrial film-coating process.  相似文献   

Fertilization and Development of Eggs of the Sea Urchin Lytechinus variegatus Maintained on an Extruded Feed     

Sophie B.  George John M.  Lawrence Addison L.  Lawrence John  Ford 《Journal of the World Aquaculture Society》2000,31(2):232-238

Sea urchin eggs are used extensively as models for studies in developmental and molecular biology. Developing aquaculture techniques and facilities for sea urchins would facilitate their use for this purpose and for production of young sea urchins for stock enhancement. A basic requirement for the aqua-culture of sea urchins is the availability of a feed that predictably produces eggs of a consistent, high quality. We tested an extruded feed with Lyrechinus variegatus for this purpose. Lytechinus variegatus (mean horizontal diameter 55 mm, 75 g wet weight) were collected in April 1998. Nine individuals were maintained in each of four aquaria with closed, recirculating filtered sea water. They were fed approximately 1 g extruded feedindividual per week. All feed was consumed. The mean weight of the sea urchins changed little with this feeding regime. The sea urchins spawned spontaneously on a monthly basis during water changes. Four females in February and one in March were spawned by injection with potassium chloride. Fertilization was 99–100% successful in each case. Egg diameters from these spawnings and a spontaneous spawning in April ranged from 102 × 6 to 128 × 1 μm. Gastrulation occurred in <20 h and metamorphosis after 22 to 37 d. Newly metamorphosed juveniles ranged in size from 435 × 38 to 473 × 56 μm. Egg size, larval size, the chronology of embryonic and larval development, and size of newly metamorphosed juveniles are similar to those obtained from field individuals reported in the literature. These results indicate that a predictable production of high quality eggs by sea urchins under controlled conditions in the laboratory with a prepared feed is feasible.  相似文献   

Effects of different commercial diets on growth performance,health and resistance to Tetrahymena sp. infection in guppies,Poecilia reticulata (Peters)          下载免费PDF全文

Galit Sharon  Sophie Fridman  Nitzan Reiss‐Hevlin  Tamar Sinai  Pascal Boisot  Dina Zilberg 《Aquaculture Research》2016,47(7):2276-2286

The effects of feeding guppy fry, Poecilia reticulata (Peters), different commercial diets from BernAqua [MeM (R = Regular, O = Ornamental and P = Premium) and experimental feed (EF] and Ocean Nutrition (ON) on growth was tested for 57 days. Health status and resistance to Tetrahymena infection were analysed at the end of the trial. The highest growth was obtained in fish fed MeM Regular and MeM Premium (mean final weights of 427 and 417 mg respectively). Fish fed EF had a significantly higher rate of spinal deformity (12.7%) than all other feeding groups (3.05% or less). Histological analysis revealed the accumulation of liver glycogen and/or lipid in fish from all feeding groups; fat accumulation in the abdomen was most pronounced in the EF and MeM Regular‐fed fish, and muscle dystrophy was observed in ca. 50% of the fish from all feeding groups except the MeM Ornamental‐fed group. The highest mortality following Tetrahymena infection occurred in fish fed EF (87%) and the lowest mortality in the MeM Ornamental‐fed fish (58%). Mortalities in all other feeding groups ranged between 69% and 76%. In summary, MeM Ornamental feed provided a moderate weight gain, no muscle dystrophy, negligible deformity and the greatest resistance to parasitic infection.  相似文献   

Population structure of short‐finned pilot whales in the oceanic archipelago of Madeira based on photo‐identification and genetic analyses: implications for conservation     

Filipe Alves  Sophie Quérouil  Ana Dinis  Cátia Nicolau  Cláudia Ribeiro  Luís Freitas  Manfred Kaufmann  Caterina Fortuna 《水产资源保护:海洋与淡水生态系统》2013,23(5):758-776

Mass spectrometry-based metabolomics to elucidate functions in marine organisms and ecosystems     

Goulitquer S  Potin P  Tonon T 《Marine drugs》2012,10(4):849-880

Marine systems are very diverse and recognized as being sources of a wide range of biomolecules. This review provides an overview of metabolite profiling based on mass spectrometry (MS) approaches in marine organisms and their environments, focusing on recent advances in the field. We also point out some of the technical challenges that need to be overcome in order to increase applications of metabolomics in marine systems, including extraction of chemical compounds from different matrices and data management. Metabolites being important links between genotype and phenotype, we describe added value provided by integration of data from metabolite profiling with other layers of omics, as well as their importance for the development of systems biology approaches in marine systems to study several biological processes, and to analyze interactions between organisms within communities. The growing importance of MS-based metabolomics in chemical ecology studies in marine ecosystems is also illustrated.  相似文献   

Genetic variation, mating patterns and gene flow in a Pinus pinaster Aiton clonal seed orchard     

Lúcia Fernandes  Margarida Rocheta  Jorge Cordeiro  Sandra Pereira  Sophie Gerber  Maria M. Oliveira  Maria M. Ribeiro 《Annals of Forest Science》2008,65(7):706-706

? Relatedness among parents, variation in clonal fertility and background pollination deviate the realized genetic gain and the gene diversity of open pollinated seed orchard from expectation, in particular in wind pollinated species such as Pinus pinaster Aiton.

? This work investigates the genetic variation, the mating system and the pollen contamination in a P. pinaster clonal seed orchard (CSO), by screening the 60 clones from the CSO and the seeds collected from 21 mother-trees with three nuclear microsatellites.

? The expected diversity was similar, but the observed heterozygosity decreased 20% in the progenies compared with the parental trees. The outcrossing rate was 90.1%, the biparental inbreeding 21.7% computed through a multilocus approach, and the observed selfing 3.9%. The observed gene flow from outside the CSO was 52.4%.

? From the results we concluded that the observed gene flow and the biparental inbreeding were high, and care should be taken in the implementation and management of future CSO, in particular clones should be checked for relatedness and the ramet number could be directly proportional to their breeding value.

  相似文献   

Sulphur immobilization and arylsulphatase activity in two calcareous arable and fallow soils as affected by glucose additions     

Phuy-Chhoy Vong  Séverine Piutti  Sophie Slezack-Deschaumes  Emile Benizri  Armand Guckert 《Geoderma》2008,148(1):79-84

In this study we examined the effects of glucose-C on the activities of fungi and bacteria determined by the method of substrate-induced respiration (SIR) in combination with the selective inhibition technique, the immobilized-S and the arylsulphatase (ARS) activity in two calcareous arable and fallow soils. The amounts of glucose-C were added at six doses: 0, 125, 250, 500, 750 and 1000 mg kg^− 1 soil to the soils and then incubated for one week with a Na₂³⁵SO₄ solution (518.9 kBq kg^− 1 dry soil and 20 mg S kg^− 1 dry soil) prior to analysis. At the highest dose of 1000 mg kg^− 1 soil, fungal activity increased by 59.1% (of the dose 0) versus 45.5% for bacterial activity in the arable soil, while in the fallow soil the increases were more marked and corresponded to 69.9% and 71.1%, respectively. Largest increase in immobilized-S was observed in the arable soil (300.7%) compared with the fallow soil (153.1%). In contrast, the ARS activity increased by 16.4% in the arable soil versus 32.1% in the fallow soil. These results indicate that glucose proportionately affected more the intensities of immobilized-S than those of ARS. Strong positive correlation coefficients were found between fungal activities and immobilized-S in the arable soil (r = 0.96, P < 0.01) and in the fallow soil (r = 0.98, P < 0.001). However, non-significant correlations were observed between fungal activities and ARS in both studied soils. As to bacterial activities, positive significant correlation coefficients were found with immobilized-S in the arable soil (r = 0.95, P < 0.01) and in the fallow soil (r = 0.90, P < 0.05) as well as with ARS activities in the arable soil (r = 0.83, P < 0.05) and in the fallow soil (r = 0.97, P < 0.01). Overall, we also found positive and significant correlation coefficients of immobilized-S with ARS activities in the arable soil (r = 0.86, P < 0.05) and in the fallow soil (r = 0.83, P < 0.05). Accordingly, the results showed a presence of extracellular arylsulphatase activity of 38.7 mg p-nitrophenol kg^− 1 soil h^− 1 in the arable soil and of 63.5 mg p-nitrophenol kg^− 1 soil h^− 1 in the fallow soil. It was concluded that fallowing maintained larger activities of fungi, bacteria and arylsulphatase compared with the arable soil.  相似文献