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11.
Mani SK Fienberg AA O'Callaghan JP Snyder GL Allen PB Dash PK Moore AN Mitchell AJ Bibb J Greengard P O'Malley BW 《Science (New York, N.Y.)》2000,287(5455):1053-1056
DARPP-32, a dopamine- and adenosine 3',5'-monophosphate (cAMP)-regulated phosphoprotein (32 kilodaltons in size), is an obligate intermediate in progesterone (P)-facilitated sexual receptivity in female rats and mice. The facilitative effect of P on sexual receptivity in female rats was blocked by antisense oligonucleotides to DARPP-32. Homozygous mice carrying a null mutation for the DARPP-32 gene exhibited minimal levels of P-facilitated sexual receptivity when compared to their wild-type littermates. P significantly increased hypothalamic cAMP levels and cAMP-dependent protein kinase activity. These increases were not inhibited by a D1 subclass dopamine receptor antagonist. P also enhanced phosphorylation of DARPP-32 on threonine 34 in the hypothalamus of mice. DARPP-32 activation is thus an obligatory step in progestin receptor regulation of sexual receptivity in rats and mice. 相似文献
12.
The physical and electrical properties of ion-implanted silicon annealed with high-powered laser radiation are described. Particular emphasis is placed on the comparison of materials properties that can be achieved with laser annealing to those which can be achieved by conventional thermal annealing. Applications of these techniques to the fabrication of high-efficiency solar cells, and potential applications of this new technique to other materials areas are discussed. 相似文献
13.
We determined some basic properties of stars that produce spectacular gamma-ray bursts at the end of their lives. We assumed that accretion of the outer portion of the stellar core by a central black hole fuels the prompt emission and that fall-back and accretion of the stellar envelope later produce the plateau in the x-ray light curve seen in some bursts. Using x-ray data for three bursts, we estimated the radius of the stellar core to be approximately (1 - 3) x 10(10) cm and that of the stellar envelope to be approximately (1 - 2) x 10(11) cm. The density profile in the envelope is fairly shallow, with rho approximately r(-2) (where rho is density and r is distance from the center of the explosion). The rotation speeds of the core and envelope are approximately 0.05 and approximately 0.2 of the local Keplerian speed, respectively. 相似文献
14.
Several instances of multiple imaging of cosmologically distant sources by intervening galaxies and galaxy clusters have been discovered over the past decade. These "gravitational lenses" have distinctive optical properties. Pointlike sources such as quasars generally produce two or four images when lensed, whereas extended sources such as galaxies produce spectacular arcs and rings. The salient features of most of the observations can be reproduced with the use of simple elliptical lens models that approximate the lenses made by ellipsoidal mass distributions such as are common in the universe. In addition to illustrating simple optics in operation on a cosmological scale, multiple images and arcs provide useful probes of the lensing galaxies and clusters. Also, gravitational lenses can make magnified images of cosmologically distant sources and may eventually furnish important cosmographic data such as the Hubble constant. 相似文献
15.
Padmanav Routray Surjya Narayan Dash Chidananda Dash Priyabrat Swain Sampad Kumar Sarkar Niranjan Sarangi 《Aquaculture Research》2008,39(15):1597-1605
The effects of extender composition, cryoprotectant concentration and freezing and thawing on the fertilization efficiency of cryopreserved spermatozoa of Puntius gonionotus were evaluated. Computer‐aided motility analysis of semen was conducted to check the suitability of spermatozoa for cryopreservation after mixing with different extenders and cryoprotective agents (CPAs). Extender‐4 with an osmolality 260 mOsmol kg−1and pH 7.6 was used for the cryopreservation study. Among the CPAs, dimethyl sulphoxide (DMSO) was least toxic and more than 60% fertilization was achieved when used at 1.4 M at 0 °C for 10 and 30 min, whereas the toxicity of all CPAs to spermatozoa was evident when tested at 30 °C. Semen frozen at −16 °C min−1 with 1.4 M DMSO showed 70% fertilization, which was significantly higher (P<0.05) than other freezing rates. Samples thawed at 35 °C water showed a fertilization rate comparable with that of fresh semen. Computer‐assisted semen analysis of fresh and frozen semen after thawing showed variations in different types of motility in spermatozoa and in their class. There was no significant difference in motility before or after cryopreservation; however, significant differences could be observed in the average path velocity (VAP), straight line velocity (VSL) and curve linear velocity (VCL). Semen of silver barb could be cryopreserved with extender‐4 by addition of 1.4 M DMSO to a final cryopreservation medium (MED 2) cooled at a rate of −16 °C min−1, stored in liquid nitrogen (−196 °C) and utilized after thawing at 35±2 °C. 相似文献
16.
T.L.W. Rothwell N. Anderson K.C. Bremner K.M. Dash L.F. Le Jambre G.C. Merritt B.K.Y. Ng 《Veterinary parasitology》1976,1(3):221-230
Sera from hosts infected with a variety of nematodes were examined for the presence of antibodies against nematode acetylcholinesterase (AChE). Antibodies were detected in the serum from hosts infected with Oesophagostomum spp., Ostertagia spp. and Trichostrongylus spp., but not in serum from hosts infected with Cooperia pectinata or Haemonchus spp., Toxocara spp. or Trichuris vulpis. In those infections in which anti-AChE antibodies were found, some individual animals failed to produce detectable antibodies. The enzyme appeared to possess antigenic specificity at the genus but not the species level. 相似文献
17.
18.
A D Narayan 《British poultry science》1976,17(2):225-230
1. The parental inbreeding component of body weight and daily gain was partitioned into paternal and maternal inbreeding components and the latter were found to be the mor important. 2. The contribution of the maternal inbreeding to the depression of characters was more pronounced on 1-d-old chicks, but declined as the chicks grew. 3. The body weight taken near sexual maturity showed a low and non-significant decline. 4. In spite of differences in the body weight of the two populations of Japanese quail the inbreeding components were similar. 相似文献
19.
K.M. Dash 《Veterinary parasitology》1981,9(2):145-149
There was no difference in the establishment or rate of development of Oesophagostomum columbianum in young lambs after infective larvae were administered either orally or by injection directly into the rumen. However, the linear distributions in the intestine of encysted third-stage larvae differed according to the route of infection. The distributions of fourth-stage larvae, after migration to the large intestine, did not differ. It is suggested that the distribution of parasitic third-stage O. columbianum is host-dominated, depending on the site of exsheathment and the rate of passage of ingesta, but that of fourth-stage and adult worms involves active site selection by the parasite. 相似文献
20.