Expression of IL-6 mRNA in endometrium with endometriosis     

ZHENG Hui  LI Hong-yi  WANG Zi-neng  ZHAO Ying-she  YU Li  HE Si-cun  BAI Zhi-quan  ZHOU Zuo-yan  YAO Ping  WANG Yue-chun 《园艺学报》2002,18(5):553-555

AIM: The purpose of the present study was to explore the relationship between interleukin-6 mRNA expression and endometriosis. METHODS: Using the rat model, IL-6 mRNA expression in the endometrium was examined by RT-PCR. RESULTS: The expression of IL-6 mRNA in control rats did not change at 2, 4, 6 and 8 weeks after sham operation (P>0.05), but in model rats it gradually increased at 2, 4, 6 and 8 weeks after endometriosis (P<0.01). The expression of IL-6 mRNA in uterine endometrium with endometriosis was lower than in endometriotic tissue, but higher than in endometrium from healthy controls. CONCLUSION: The IL-6 mRNA expression may contribute to the development of endometriosis . The increase in IL-6 mRNA expression may promote the implantation and growth of endometriotic tissue.  相似文献   

Effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts     

REN Yu-sheng  WU Zong-gui  CUI Fang  JIA Guo-liang  YU Shi-qing  TANG Chao-wu  LI Bo 《园艺学报》2002,18(11):1377-1380

AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using [³H]-TdR incorporation and [³H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L^-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts.  相似文献   

Protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes     

ZHANG Yu-xia  YU Lun-yin  LIU Ming-qiu  ZHANG Zheng-bin TANG Zhi-jiao  XIA Dong  WANG Ming 《园艺学报》2002,18(1):32-35

AIM:To investigate the protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS:One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D₂ and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS:①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D₂ in 3,4,5 day CM group was 0.89 times(P＜0.05),0.80 times (P＜0.05) and 0.56 times (P＜0.01) of that in 1 day group, respectively. The expression of p16 in CM was increased during the culture process, 2,3,4,5 day group were 1.63 times, 1.72 times, 1.99 times and 2.84 times (P＜0.01) of that in 1 day group, respectively.CONCLUSION:Cultured neonatal rat cardiac myocytes could proliferate during the first 3 days after incubation, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D₂ and p16 play the key roles in CM postnatal development. Downregulation of cyclin D₂ and upregulation of p16 may induce CM differentiation.  相似文献   

钻蛀板栗枝干害虫的发生情况分析     

俞云祥  黄素红  丁冬荪 《江西植保》2003,26(2):59-61

本文对钻蛀板栗枝干害虫的发生分布、致树木死亡的发生量和不同树龄期的发生差异等情况进行了分析。从中找到了一些规律，可供有关方面应用。  相似文献   

螟虫长距茧蜂研究进展     

张安盛  冯建国于毅 张思聪 《昆虫天敌》2003,25(2):89-92

本文综述了对螟虫长距茧蜂的研究概况，包括该寄生蜂的主要生物学习性、对玉米螟自然寄生率及田间防治玉米螟效果等。  相似文献   

热胁迫对辣椒花柱细胞中Ca²⁺ 分布的影响   总被引：5，自引：0，他引：5  

阎春兰  王建波 《园艺学报》2003,30(1):95-97

 用焦锑酸钾沉淀法研究了热胁迫对辣椒开花前后柱头和花柱细胞中Ca²⁺分布的影响。结果表明: 常温下Ca²⁺ 分布在柱头细胞表面、花柱引导组织细胞间隙等质外体系统中, 且在花粉管上呈先增加后减少的规律性变化; 热胁迫后, 除细胞间隙等质外体系统外, 细胞质和细胞核中也出现Ca²⁺, 花柱引导组织中通过的花粉管发生扭曲、变形, 这可能与Ca²⁺ 的异常分布有关。  相似文献   

检测猪流行性腹泻病毒的R-PCR方法的建立   总被引：1，自引：0，他引：1  

倪艳秀  何孔旺  俞正玉  郭蓉利  吕立新  陈兆霞 《畜牧与兽医》2004,36(1):10-12

根据猪流行性腹泻病毒 (PEDV)的N基因自行设计和合成了一对可扩增长度为 641bp目的片段的引物 ,成功地建立了检测的猪流行性腹泻病毒的RT PCR方法。对猪轮状病毒 (PRV)、猪传染性胃肠炎病毒 (TGEV)的RT PCR检测结果均呈阴性。对PEDV JS株的RT PCR产物的序列分析表明 ,与CV777株的同源性为 97 3 %。  相似文献   

差异显示法研究不同锌源对组织基因表达的影响     

虞泽鹏  乐国伟  施用晖 《畜牧与兽医》2004,36(4):4-6

选用Avine肉用仔鸡作为试验对象 ,饲喂硫酸锌和蛋氨酸锌 2种锌源。采用银染差异显示技术进行差异基因的初步筛选。结果成功回收扩增差异条带 2 6条 ;其中加锌组 (硫酸锌组和蛋氨酸锌组 ) 2 3条 ,3条为缺锌组所特有。在加锌组的 2 3条中 ,6条为蛋氨酸锌组所特有 ,1 3条为硫酸锌组所特有 ,其余 4条为 2组所共有。这些初步筛选得到的差异基因尚需进一步进行克隆、测序和杂交进行验证。  相似文献   

利用废菌糠提高覆土持水力和蘑菇产量的研究   总被引：10，自引：1，他引：10  

王志强  郭倩  凌霞芬  郁建强 《中国食用菌》2004,23(5):15-17

本文利用平菇等食用菌的废菌糠 ,经适当无氧发酵等一系列处理后添加到普通泥土中 ,使其最大持水力提高至 5 1 3% ,单产提高 13 6 %  相似文献   

49种植物源粗提物对黄瓜灰霉菌的生物活性筛选   总被引：8，自引：0，他引：8  

喻大昭  杨小军  杨立军  王少南  张汉亦 《植物保护学报》2004,31(2):217-218

黄瓜灰霉病是由灰霉菌Botrytis cinerea引起的重要病害.目前,尚无理想的抗灰霉病黄瓜品种,药剂防治仍然是控制该病害的必要措施.20世纪80年代以前防治灰霉病的杀菌剂如苯并咪唑类的多菌灵、硫代氨基甲酸酯类的甲基托布津、二羧酰亚胺类的异菌脲、腐霉利等已使用了近30年,灰霉病菌对这些药剂均产生了不同程度的抗药性.作者测定了植物源粗提物对黄瓜灰霉病菌的活性,以期研制开发出无公害植物源杀菌剂.  相似文献