几种绢丝昆虫遗传多样性的RAPD研究   总被引：10，自引：4，他引：6  

程道军  鲁成  周泽扬  向仲怀 《蚕业科学》2002,28(4):277-282

选用重复性较好的 10个引物对 6种绢丝昆虫 2 3个个体进行扩增 ,共得到 2 45个RAPD标记。RAPD分析结果表明 :家蚕及野桑蚕种内各品种材料的遗传距离较大 ,天蚕、蓖麻蚕及柞蚕较小 ;家蚕与野桑蚕两者之间遗传距离小 ,反映出家蚕和野桑蚕亲缘关系较近 ;而天蚕、蓖麻蚕和柞蚕三者之间以及分别与家蚕和野桑蚕之间的遗传距离较大 ,亲缘关系远。聚类分析的结果与之相同。并且对家蚕的起源及绢丝昆虫遗传多样性的保护利用做了探讨。  相似文献   

灭火作战中火场紧急避险“六要素”   总被引：1，自引：0，他引：1  

周昌荣 《森林防火》2006,(4):34-35

针对灭火作战中火场紧急避险,提出了注重科学指挥;加强日常避险训练习;抓好火场紧急避险常识教育;加强火场紧急避险理论研究工作;灭火作战任务与安全避险统筹兼顾;熟悉战法灵活避险六点看法。  相似文献   

Management of parthenium weed through competitive displacement with beneficial plants     

CHRIS O'DONNELL  STEVE W. ADKINS 《Weed Biology and Management》2005,5(2):77-79

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南、北盘江流域（贵州部分）土地退化类型及机理   总被引：1，自引：0，他引：1  

周家维 《贵州林业科技》2005,33(4):6-10

在对珠江上游南、北盘江流域（贵州部分）调查的基础上，分析了该流域土地退化成因，原生生态系统的脆弱性只是导致土地退化的一个方面。而人为活动才是土地退化的主要因素。根据土地退化的成因、结果，将珠江上游南、北盘江流域（贵州部分）土地退化划分为：土壤侵蚀、石漠化、土地贫瘠、土壤污染、土壤破坏五种类型，并提出了退化土地的管理及恢复措施。  相似文献   

湖南省滑胸茧蜂属Homolobus Foerster初报(膜翅目:茧蜂科:滑胸茧蜂亚科)     

游兰韶  周至宏  童新旺 《湖南农业大学学报(自然科学版)》1990,(4)

描记滑胸茧蜂属属征和褐滑胸茧蜂形态,并提供该蜂的形态图。  相似文献   

Nuclear transfer in loach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion     

F Hongtuo  & W Chingjiang  . 《Aquaculture Research》2001,32(4):267-275

To study nuclear transfer in the loach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 °C in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days. It was found that gastrula cells incubated at 4 °C had the same totipotence as blastula cells. The optimal UV dosage for inactivation of the oocyte chromatin was 180–240 mJ cm⁻². Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.  相似文献   

Outcome of limb amputations in wapiti: 13 cases (1995-2001).          下载免费PDF全文

T D Butt  A M Cruz  J V Bailey    W H Crawford 《The Canadian veterinary journal. La revue veterinaire canadienne》2001,42(12):936-939

The object of this investigation was to determine the outcome of limb amputation in wapiti. Medical records of 13 wapiti that underwent limb amputation were reviewed to determine age, weight, sex, injury preceding amputation, limb amputated, amputation location, length of hospital stay, complications, and outcome. Ages ranged from 3 months to 8 years and weights from 70 kg to 280 kg. All animals were female. Eleven animals sustained catastrophic long bone fractures prior to amputation. Five animals had front limb amputations and 8 had hind limb amputations. Of these animals, 1 with a front limb amputaiton and 5 with hind limb amputations survived. Postsurgical inability to stand is associated with hospitalization of > 1 day. Of the 13 wapiti reviewed, 5 with hind limb amputations have produced and reared offspring. Hind limb amputation can be considered a viable alternative to euthanasia when catastrophic orthopedic injuries occur in female wapiti.  相似文献   

Immunity of specific pathogen-free lambs to challenge with an aerosol of Pasteurella haemolytica biotype A serotype 2. Pulmonary antibody and cell responses to primary and secondary infections     

W Donachie  C Burrells  A D Sutherland  J S Gilmour  N J Gilmour 《Veterinary immunology and immunopathology》1986,11(3):265-279

Specific pathogen-free (SPF) lambs previously exposed to an aerosol of P. haemolytica biotype A serotype 2 (A2) were immune to subsequent challenge with an aerosol of P. haemolytica A2. Untreated control lambs were not immune to this challenge. The local immune responses of the lung to these challenges were examined. High IgG and IgA titres to P. haemolytica and high levels of opsonizing antibody against P. haemolytica were present in the lung washings from previously infected immune lambs at autopsy, seven days after the second infection. Lung washings from control lambs, 7 days after challenge with P13 virus and P. haemolytica A2, had no IgG titres, very little opsonizing activity but did have IgA titres which were significantly higher than in unchallenged control lambs. The cellular response of animals challenged with P13 virus and P. haemolytica was significantly greater than that of unchallenged controls or of lambs exposed only to P. haemolytica. However, this finding was complicated by the response to P13 virus. Lymphocytes from lung washings of all lambs failed to respond in a lymphocyte stimulation test to phytohaemagglutinin while blood lymphocytes did respond. There was little specific response to P. haemolytica antigen in the test.  相似文献   

Genetic diversity estimates in Cicer using AFLP analysis   总被引：2，自引：0，他引：2  

T. T. NGUYEN  P. W. J. TAYLOR  R. J. REDDEN  R. FORD 《Plant Breeding》2004,123(2):173-179

Amplified fragment length polymorphism (AFLP) analysis was used to evaluate the genetic variation among cultivated chickpea and wild Cicer relatives. In total, 214 marker loci were assessed, of which 211 were polymorphic (98.6%) across the 95 accessions that represented 17 species of Cicer. The genetic variation within a species was highest in C. pinnatifidum followed by C. reticulatum and lowest in C. macracanthum. Three main species groups were identified by UPGMA clustering using Nei's pair‐wise distance calculations. Group I included the cultivated species C. arietinum, C. reticulatum and C. echinospermum. Within this group, C. reticulatum accessions were clustered closest to the C. arietinum cultivars ‘Lasseter’, ‘Kaniva’ and ‘Bumper’, supporting the hypothesis that C. reticulatum is the most probable progenitor of the cultivated species. Cicer bijugum, C. judaicum and C. pinnatifidum were clustered together creating group II. Group III contained all nine perennial species assessed and two annual species C. yamashitae and C. cuneatum. The genetic distance detected between group I and group III (0.13) was equivalent to the genetic distance detected between group I and group II (the primary and annual tertiary species, respectively; 0.14). This indicated that the perennial tertiary species may be as valuable for increasing variation to incorporate novel germplasm in the cultigen as the annual tertiary species.  相似文献   

Infectious disease serologic survey in free-ranging Venezuelan anacondas (Eunectes murinus).   总被引：1，自引：0，他引：1  

P P Calle  J Rivas  M Mu?oz  J Thorbjarnarson  W Holmstrom  W B Karesh 《Journal of zoo and wildlife medicine》2001,32(3):320-323

Reptiles can harbor pathogenic microorganisms asymptomatically and serve as potential reservoirs of infection for humans, domestic animals, and other reptiles. Infectious diseases are also problematic for free-ranging reptile populations and are an important consideration in reptile reintroduction and translocation projects. There have been limited serologic studies of free-ranging reptiles for evidence of exposure to potential pathogens. In the present study, serum or plasma samples from five male and five female free-ranging Venezuelan anacondas (Eunectes murinus) were screened for antibodies to eastern, western, and Venezuelan equine encephalitis viruses, vesicular stomatitis virus, ophidian paramyxovirus, 19 Leptospira interrogans serovars, and Cryptosporidium serpentes. Antibodies to these agents were not detected, or antibody titers were low and possibly nonspecific. These results for the limited number of anacondas surveyed suggest that they do not serve as significant reservoirs for these infectious agents at this location.  相似文献