Qualitative and Quantitative Contrast Enhanced Ultrasonography of the Pancreas Using Bolus Injection and Continuous Infusion Methods in Normal Dogs     

Sue Yee LIM  Kensuke NAKAMURA  Keitaro MORISHITA  Noboru SASAKI  Masahiro MURAKAMI  Tatsuyuki OSUGA  Hiroshi OHTA  Masahiro YAMASAKI  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1601-1607

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Nucleotide polymorphisms and the 5′‐UTR transcriptional analysis of the bovine growth hormone secretagogue receptor 1a (GHSR1a) gene     

Masanori KOMATSU  Yuki FUJIMORI  Yoichi SATO  Hiroaki OKAMURA  Seiki SASAKI  Tomohito ITOH  Mitsuo MORITA  Ryoichi NAKAMURA  Toshiaki OE  Masako FURUTA  Junpei YASUDA  Takatoshi KOJIMA  Toshio WATANABE  Takeshi HAYASHI  Aduli E.O. MALAU‐ADULI  Hideaki TAKAHASHI 《Animal Science Journal》2010,81(5):530-550

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Mobility of the forearm skeleton in the Asiatic black (Ursus thibetanus), brown (U. arctos) and polar (U. maritimus) bears     

Hayato AMAIKE  Motoki SASAKI  Nao TSUZUKI  Mitsunori KAYANO  Motoharu OISHI  Kazutaka YAMADA  Hideki ENDO  Tomoko ANEZAKI  Naoya MATSUMOTO  Rumiko NAKASHITA  Misako KUROE  Hajime TARU  Gen BANDO  Yuko IKETANI  Ryohei NAKAMURA  Nobutaka SATO  Daisuke FUKUI  Nobuo KITAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1284

In several primates and carnivores, pronation/supination angles of the forearm skeleton were examined, and it is thought that a larger angle is useful to acquire dexterous behaviors in feeding and/or life style, including climbing. In this study, the pronation/supination angles in Asiatic black, brown and polar bears were nondestructively examined. These specimens were classified as adult or non-adult. Three or four carcasses of each group of Asiatic black and brown bears were used for CT analysis, whereas only one adult polar bear was used. The forearms were positioned within the gantry of a CT scanner in both maximally supinated and pronated states. Extracted cross-sectional CT images of two positions were superimposed by overlapping the outlines of each ulna. The centroids of the radii were detected, and then the centroid of each radius and the midpoint of a line which connects between both ends of the surface of each radius facing the ulna, were connected by lines to measure the angle of rotation as an index of pronation/supination. In adult brown and polar bears, the angles were smaller as compared with the other groups (Asiatic black and non-adult brown bears). Asiatic black and non-adult brown bears can climb trees, whereas adult brown bears and polar bears cannot. This suggests that the pronation/supination angle is related to arboreal activity in Ursidae.  相似文献   

Feasibility for use of digested slurry by the pouring method in paddy fields of Southern Vietnam     

Fumiko Oritate  Masato Nakamura  Dan Phuoc Nguyen  Hanh Vu Bich Dang  Khanh Duy Nguyen  Yoshito Yuyama  Masaru Yamaoka  Iwao Kitagawa  Akiyoshi Sakoda  Kazuhiro Mochidzuki 《Paddy and Water Environment》2016,14(4):429-438

In this study, we evaluated the feasibility for the use of digested slurry from livestock manure (hereafter, slurry) in paddy fields through field experiments conducted in Southern Vietnam. The pouring method for slurry was used, and a vacuum truck was used for transportation and pouring of the slurry. A prototype slurry tanker was manufactured for transportation and application of slurry, because vacuum trucks are rarely available in rural areas of Vietnam. For evaluation of feasibility, costs and labor for application of slurry and rice production were examined and compared with conventional cultivation methods using chemical fertilizers. As the results, rice production with the use of slurry was 485 g m^?2, which is within the range of on-site conventional cultivation, so slurry may be a good substitute for chemical fertilizers in rice production. Costs for slurry fertilization with a prototype slurry tanker and a vacuum truck were estimated at 0.13 USD m^?2 and 0.10 USD m^?2, respectively. These costs were higher than for conventional cultivation of 0.06 USD m^?2 under the present conditions with T-N concentrations of approximately 400 mg L^?1 in the slurry. However, we clarified that the cost for slurry fertilization can be lower than conventional cultivation when the concentrations of nitrogen in slurry increase from 400 to 2000 mg L^?1. These results show that an increase in nitrogen concentrations in slurry make slurry fertilization feasible if the amounts of water for washing livestock sheds that enter into the biogas digesters are reduced.  相似文献   

Aldehyde dehydrogenase activity in cancer stem cells from canine mammary carcinoma cell lines     

Michishita M  Akiyoshi R  Suemizu H  Nakagawa T  Sasaki N  Takemitsu H  Arai T  Takahashi K 《Veterinary journal (London, England : 1997)》2012,193(2):508-513

Increasing evidence suggests that diverse solid tumours arise from a small population of cells known as cancer stem cells or tumour-initiating cells. Cancer stem cells in several solid tumours are enriched for aldehyde dehydrogenase (ALDH) activity. High levels of ALDH activity (ALDH(high)) were detected in four cell lines derived from canine mammary carcinomas. ALDH(high) cells were enriched in a CD44(+)CD24(-) population having self-renewal capacity. Xenotransplantation into immunodeficient mice demonstrated that 1×10(4) ALDH(high) cells were sufficient for tumour formation in all injected mice, whereas 1×10(4) ALDH(low) cells failed to initiate any tumours. ALDH(high)-derived tumours contained both ALDH(+) and ALDH(-) cells, indicating that these cells had cancer stem cell-like properties.  相似文献   

Time‐coordinated prevalence of extracellular HGF,FGF2 and TGF‐β3 in crush‐injured skeletal muscle     

Mai‐Khoi Q. DO  Takahiro SUZUKI  Borjigin GERELT  Yusuke SATO  Wataru MIZUNOYA  Mako NAKAMURA  Yoshihide IKEUCHI  Judy E. ANDERSON  Ryuichi TATSUMI 《Animal Science Journal》2012,83(10):712-717

Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon‐guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early‐differentiated satellite cells up‐regulate a secreted neural chemorepellent Sema3A upon in vivo muscle‐crush injury. The study also revealed that Sema3A expression is up‐regulated in primary satellite‐cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)‐β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time‐course of extracellular HGF, FGF2 and TGF‐β3 concentrations after crush‐injury of Gastrocnemius muscle in the rat lower hind‐limb, using a combination of a non‐homogenization/non‐spin extraction of extracellular wound fluids and enhanced chemiluminescence–Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2–8 days post‐crush, whereas active TGF‐β3 increases after 12 days, providing a better understanding of the time‐coordinated levels of HGF, FGF2 and TGF‐β3 that drive regulation of Sema3A expression during regenerative intramuscular moto‐neuritogenesis.  相似文献   

Evaluation of the polymorphonuclear cell functions of bottlenose dolphins     

Noda K  Aoki M  Akiyoshi H  Asaki H  Shimada T  Ohashi F 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(6):727-729

The functions of polymorphonuclear cells (PMN) are the important non-specific defense mechanisms in the immune system. Especially marine mammals are protected by these mechanisms from the aquatic environment with a large variety of microorganisms. Therefore, we examined the PMN functions of bottlenose dolphins in order to obtain the normal ranges and to standardize the techniques. PMNs were isolated by using lymphocyte isolate solution whose density was 1.077; superoxide production was assessed by nitroblue tetrazolium reduction test (NBT) and phagocytosis was tested by using polystyrene latex beads. We showed that the optimal incubation time was 30 min in NBT assay and 12 hr in phagocytosis assay for dolphin PMNs.  相似文献   

An investigation of antibody response in cattle vaccinated with the rabbit-passaged LA rinderpest virus in Korea.     

J NAKAMURA  S KISHI  J KIUCHI  R REISINGER 《American journal of veterinary research》1955,16(58):71-75

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Experimental production of porcine gastroesophageal ulcers by betazole and reserpine     

EI-ICHI KOKUE  TAKASHI NAKAMURA  TOYOAKI HAYAMA 《Journal of veterinary pharmacology and therapeutics》1978,1(3):217-224

The ulcerogenic and secretogenic properties of betazole, (an H₂ agonist), and/or reserpine on pig stomachs were studied. (1) Betazole (50 mg/body) and reserpine (0.02 mg/kg) were successively injected (i.m.) for 5–10 days into starved animals. Gastroesophageal ulcers, pathologically similar to naturally occurring ulcers were observed in all pigs (10/10), whether a nutrient solution (5% glucose, 1 litre/day) was supplied or not. In the starved pigs given either betazole or reserpine, only a small ulcer or erosion was observed. (2) In four pigs which were given commercial mash at a rate of 3% of body weight per day and killed on the 6th or 10th day of successive injections of both drugs, the incidence of ulcers was 2/4. (3) The gastric secretogenic effect of betazole and/or reserpine was confirmed in pigs with Heidenhain gastric pouches. (4) From these data, a new method for the experimental induction of pig gastroesophageal ulcers, by means of simultaneous injections of betazole and reserpine for 5 days under the nutrient (glucose solution) drinking condition, is proposed. The authors consider that this method may be superior to the repository histamine method, with respect to the absence of adverse effects, practical convenience and pathological and etiological similarities with naturally occurring ulcers.  相似文献   

Functional indispensability of fibronectin associated on the mesenchymal cell surface during the early period of feather development in the chick embryo in vitro     

Ken KOBAYASHI  Shigeharu FUKUNAGA  Kazuaki TAKENOUCHI  Fumio NAKAMURA 《Animal Science Journal》2004,75(5):441-449

To clarify the role of fibronectin (FN) during the early period of feather development, reconstituted skin consisting of intact epithelium and isolated mesenchymal cells from embryonal chick skin was used. In early feather development, FN was localized around mesenchymal cells of the dermal condensation. Isolated mesenchymal cells had associated with FN on their surfaces. FN on the cell surface dissociated following EDTA treatment, and EDTA‐treated cells re‐associated with exogenous FN. The intact epithelium also bound to exogenous FN at the placode. When FN‐associated or FN‐reassociated mesenchymal cells were used, the reconstituted skin formed feather rudiments only at the positions where the epithelial placode existed originally, and the locality of tenascin transferred from the placode to the mesenchyme during the period of feather bud formation. However, in reconstituted skin using FN‐dissociated mesenchymal cells, feather rudiments did not form. Additionally, the epithelial placodes disappeared, and tenascin was distributed uniformly on the surface of the epithelium and not localized in the mesenchyme. These findings suggest that FN associated on the surfaces of mesenchymal cells maintains the functions of mesenchymal cells as dermal condensation and mediates epithelial‐mesenchymal interactions during the early period of feather development. The results also suggest that reconstituted skin is a useful tool for functional studies on the extracellular matrix.  相似文献