Effects of Antrodia camphorata on alcohol clearance and antifibrosis in livers of rats continuously fed alcohol     

Wu MT  Tzang BS  Chang YY  Chiu CH  Kang WY  Huang CH  Chen YC 《Journal of agricultural and food chemistry》2011,59(8):4248-4254

Alcoholic fatty liver disease (AFLD) is the result of an excessive or chronic consumption of alcohol. Nine male Wistar rats per group were randomly assigned to one of the following drinking treatments: a 20% (w/w) alcohol solution (ALC); a 20% (w/w) alcohol solution cotreated with 0.25 g silymarin/kg BW/day; or a 20% (w/w) alcohol solution cotreated with 0.025 g Niuchangchih ( Antrodia camphorata )/kg BW/day for 4 weeks. Rats with cotreatments of silymarin or Niuchangchih had smaller (p < 0.05) relative liver size, less (p < 0.05) liver lipid accumulation, and lower (p < 0.05) liver damage indices [aspartate aminotransferase (AST) and alkaline phosphatase (ALP) values]. In the regulation of alcohol metabolism, the lower serum alcohol level was observed only in alcohol-fed rats supplemented with Niuchangchih. Meanwhile, cotreatment of silymarin or Niuchangchih increased (p < 0.05) CAT and ALDH activities but did not (p > 0.05) affect ADH and CYP2E1 expressions, which accelerate alcohol metabolism in the body. Additionally, neither silymarin nor Niuchangchih (p > 0.05) influenced serum/hepatic MMP-2 activities and NF-κB, AP1, and α-SMA gene expressions, but serum/hepatic MMP-9 activities and TNF-α, KLF-6, and TGF-β1 gene expressions of alcohol-fed rats were down-regulated (p < 0.05) by silymarin or Niuchangchih, which also could explain the lower liver damage observed in rats chronically fed alcohol.  相似文献   

Identification and anti-human glioblastoma activity of tagitinin C from Tithonia diversifolia methanolic extract     

Lee MY  Liao MH  Tsai YN  Chiu KH  Wen HC 《Journal of agricultural and food chemistry》2011,59(6):2347-2355

The Tithonia diversifolia methanolic extract (TDM), which showed antiproliferative activity against human glioblastoma U373 cells, with an IC50 value of 59.2±3.7 μg mL(-1), was passed through silica gel chromatography and successively eluted with different percentages of EtOAc/hexane. The 10-60% EtOAc/hexane subfractions, which exhibited a comparatively higher antiproliferative activity, were isolated, and then structural identification was proceeded with 1H nuclear magnetic resonance. The isolated compound was tagitinin C, a kind of sesquiterpenoid. The IC50 value was 6.1±0.1 μg mL(-1) in U373 treated with tagitinin C. In flow cytometric analysis and inhibition of pan-caspase, the results showed that the anti-glioblastoma effect was apoptosis-independent. In PARP, p-p38, ULK1, and LC3-II expression, the anti-glioblastoma induced by tagitinin C was likely via autophagy. In the ULK1 siRNA transfection experiment, autophagy blockade counteracted the suppression induced by tagitinin C. The result suggested that tagitinin C induces U373 cell death dependent upon autophagy under certain conditions.  相似文献   

Derivation and properties of recombinant Fab antibodies to coplanar polychlorinated biphenyls     

Chiu YW  Chen R  Li QX  Karu AE 《Journal of agricultural and food chemistry》2000,48(6):2614-2624

Recombinant Fab antibodies (rFabs) specific for coplanar polychlorinated biphenyls (PCBs) were derived from a hybridoma cell line (Chiu et al. Anal. Chem. 1995, 67, 3829-3839). Immunoglobulin V(H)-C(H1) and V(L)-C(L) sequences from S2B1 messenger RNA were amplified by PCR and cloned into the M13 phagemid vector pComb3H. Phage displaying rFab were enriched by panning on a PCB hapten conjugate and expressed as soluble rFabs in Escherichia coli XL-1 Blue. Two rFab clones competitively bound PCBs 77 and 126 with half-maximal inhibition (I(50)) of 10-13 ppb in indirect and direct enzyme immunoassays (EIAs), with selectivity nearly identical to that of whole S2B1 IgG and its Fab fragments prepared by papain digestion. These results, and comparison of N-terminal amino acid sequences of MAb S2B1 and the rFab, indicated that rFab S2B1 is a functional copy of the MAb. The rFab S2B1 sequences have 75-89% sequence identity with antibodies that bind nitrophenyl haptens and are being used to construct a three-dimensional computational model of the PCB binding site.  相似文献   

一种病毒抑制物质NS-83的研制及其对番茄预防TMV初侵染的研究   总被引：9，自引：0，他引：9  

雷新云  裘维蕃  于振华  严梅和 《植物病理学报》1984,14(1):1-7

 本文论述了几种长链醇和长链酸,在番茄上对TMV初侵染的抑制作用。测试了壬醇、癸醇、十一(烷)醇、十二(烷)醇、十四醇、十六醇、十八醇等长链醇。试验的脂肪酸有二十二酸、二十二酸钠、混合脂肪酸和NS-83制剂。菜籽油中的脂肪酸含有1.1%二十二酸、35~36%顺-二十二烯-13酸(芥酸)、11~12%花生酸、12.4~12.7%亚麻酸、12.7~12.9%亚油酸、11~11.3%油酸、2.5%二十四烯酸、2%花生烯酸、4%木焦油酸、1.8~2%硬脂酸和3.1%软脂酸。这些脂肪酸的混合剂对侵染番茄的TMV的抑制作用与纯二十二酸单独的抑制作用相接近。NS-83制剂是由混合脂肪酸制备,防病效果更好,与目前其他一些抑制物质相比较,成本低廉,可用于防治番茄病毒病。此外,这种抑制物质似具有诱导番茄对TMV的初侵染以及对TMV和CMV产生耐病性的能力。其实际效果的机理,有待进一步试验,加以证明。  相似文献   

VA菌根真菌和大丽轮枝菌(Verticillium dahliae)对棉花体内PR蛋白的诱导作用   总被引：1，自引：0，他引：1  

刘润进  沈崇尧  李怀方  裘维蕃 《植物病理学报》1993,23(2):162-162

 植物对病原物侵害和多种逆境的普遍反应是合成病程相关蛋自(Pathogenesis-Related Proteins,PRs)。  相似文献   

Megakaryocytes with intracytoplasmic blood cells     

T Chiu 《American journal of veterinary research》1984,45(4):769-770

The phenomenon of megakaryocytes containing intracytoplasmic blood cells was frequently observed in the bone marrow of rats. The frequency appeared to increase in inflammatory or neoplastic conditions. The types of blood cells engulfed were mainly neutrophils. For the first time, megakaryocytes also were found to be engulfed.  相似文献   

柑桔木虱(Diaphorina citri Kuwayama)与柑桔黄龙病流行关系的初步研究   总被引：1，自引：0，他引：1  

赵学源  蒋元晖  李世菱  邱柱石  苏维芳 《植物病理学报》1979,9(2):121-126

 1973-1978年用从田间病树上采集的柑桔木虱成虫放饲在398株健康柑桔苗上,有32株发病。用病树上采集的柑桔木虱高龄若虫置健苗上所羽化的成虫放饲的56株,有5株发病。未接虫的110株没有发病。初步说明柑桔木虱成虫可以传病。  相似文献   

Ventricular pneumocephalus and septic meningoencephalitis secondary to dorsal rhinotomy and nasal polypectomy in a dog     

Fletcher DJ  Snyder JM  Messinger JS  Chiu AG  Vite CH 《Journal of the American Veterinary Medical Association》2006,229(2):240-245

CASE DESCRIPTION: A 4-year-old sexually intact female French Bulldog was evaluated because of lethargy, anorexia, and chronic rhinitis-sinusitis. The dog had nasal discharge of 18 months' duration; dorsal rhinotomies were performed 3 months and 2 weeks prior to referral. CLINICAL FINDINGS: On initial evaluation, intraventricular pneumocephalus and sinusitis were diagnosed; CSF analysis revealed high total protein concentration and mononuclear pleocytosis. The dog's condition improved with treatment. Two weeks after discharge, it was treated by a local veterinarian because of upper airway obstruction; 3 days later, the dog was referred because of seizures. Computed tomography revealed a large fluid-filled, left lateral ventricle and a soft tissue mass protruding through a cribriform plate defect. The mass was histologically consistent with brain tissue. Findings of clinicopathologic analyses were unremarkable. Results of cytologic examination of a CSF sample were indicative of septic, suppurative inflammation, and bacteriologic culture of CSF yielded Escherichia coli. TREATMENT AND OUTCOME: Amputation of the herniated olfactory bulb and antimicrobial treatment resolved the septic meningoencephalitis, but neurologic deficits recurred 6 weeks later. Definitive correction of the cribriform plate defect with bone and fascial grafts was attempted. Postoperative rotation of the bone graft resulted in cerebral laceration and hemorrhage, and the dog was euthanized. CLINICAL RELEVANCE: Findings suggest that following dorsal rhinotomy and nasal polypectomy surgery, the dog developed herniation of the left olfactory bulb, intra-ventricular pneumocephalus, and septic meningo-encephalitis because of a cribriform plate defect. Care must be taken to prevent rotation of bone grafts used in cribriform defect repair.  相似文献   

A miniature lighted stylet for fast oral endotracheal intubation in rabbits     

H.P. Su  C.J. Hou  W.H. Chen  K.J. Wang  Y.H. Chiu  H.S. Sun 《Veterinary journal (London, England : 1997)》2013,195(2):254-256

Efficient oral endotracheal intubation of laboratory animals is a challenging technique in veterinary research. This study introduces a miniaturized lighted stylet for rabbit intubation. An experiment with repeated measures on two factors was used to assess the feasibility and efficacy of this method. The first factor compared stylet intubation vs. laryngoscopic intubation. The second compared three practitioners, one with prior experience and two without. Success rates on the initial attempt were not statistically different (χ² = 2.46, P = 0.12). The time difference between methods was significant (F = 41.007, P < 0.001), although the effect of practitioners was not (F = 1.038, P = 0.365). The mean ± SD of the intubation time, combining results from the three practitioners, was 20.34 ± 17.15 s for the stylet method and 57.58 ± 64.21 s for the laryngoscopic method. The results of this study demonstrate that lighted stylet intubation is efficient, robust, and independent of practitioner experience.  相似文献   

The Extract of Rhodobacter sphaeroides Inhibits Melanogenesis through the MEK/ERK Signaling Pathway     

Wen-Sheng Liu  Yu-Diao Kuan  Kuo-Hsun Chiu  Wei-Kuang Wang  Fu-Hsin Chang  Chen-Hsun Liu  Che-Hsin Lee 《Marine drugs》2013,11(6):1899-1908

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