鸡山农场天然橡胶新品种抗性试验     

戴少军  蔡扬顺 《热带作物学报》2012,32(4):1-4

高产优质抗病甘薯新品种湛薯407是广东省湛江市农业科学研究所以龙薯1号为母本、鸡母薯为父本，通过品种间杂交、多年多点综合鉴定选育而成。2008～2009年参加广东省甘薯新品种区域试验，2010年进行大田生产试验，各试验综合性状表现较好。该品种于2011年5月通过广东省农作物新品种鉴定，鉴定编号为粤审薯2011004。对湛薯407的品质性状、抗病性、产量等进行研究，结果表明：湛薯407薯干品质和食味较好，中抗薯瘟病，鲜薯和薯干产量与广薯111相比增产极显著，适应性广，适宜广东省各薯区种植。根据该品种的生长特性，还提供配套的栽培技术。  相似文献   

综合利用资源优势开发建设果林大观园--长泰县南亚热带果林大观园的构想与对策     

林鸿兴  庄岂凡  戴宝龙 《经济林研究》2000,18(3):57-58

1　建设南亚热果林大观园的优势1.1　南亚热带气候优势长泰属南亚热带海洋性季风气候区 ,年均气温 2 1.7℃ ,平均日照差 8.6℃ ,年均积温 74 2 2 .7℃ ,无霜期 32 8天 ,年均相对湿度 78% ,年均降雨 14 6 2 mm,水热资源丰富 ,冬无严寒 ,夏无酷暑 ,终年温暖湿润。1.2　名优水果优势长泰芦柑在全国柑桔质量评比中 ,连续夺得“三连冠”,又是唯一获得绿色食品使用标志的柑桔 ,建成我国第一个晚芦基地 ,岩溪被誉为“中国芦柑之乡”,枋洋、坂里大力发展中国名柚——文旦柚 ,以水果为主的经济林达 18万亩 ,实现人均一亩园 ,年产千斤果 ,获得福建省水…  相似文献   

酶联免疫吸附测定(ELISA)检测苏云金杆菌伴孢晶体蛋白质研究   总被引：2，自引：0，他引：2  

罗绍彬  阎建平  戴顺英  刘娥英  张用梅  胡名瑞 《中国生物防治》1987,(4)

用酶联免疫吸附测定(ELISA)检测Bt伴孢晶体毒素蛋白质,最低可检值7.79ng/0.15ml,灵敏、精确,具高度专化性。测定标准品HI—1—S—1980和Bt乳剂产品中伴孢晶体蛋白质含量,分别为108.06mg/g粉剂和4.57—6.17mg/ml乳剂。用标准品与待测样品(Bt乳剂)的晶体蛋白质含量,就可计算出待测样品的相对毒力效价。ELISA与昆虫生测之间有良好的相关性。  相似文献   

表达猪链球菌溶血素基因的减毒沙门氏菌的构建及鉴定   总被引：3，自引：1，他引：3  

马有智  戴贤君  李肖梁  方维焕 《中国兽医学报》2005,25(5):478-480

将猪链球菌溶血素（suilysin，SLY）基因克隆入原核表达栽体pBV220，将重组质粒再导入减毒鼠伤寒沙门氏菌SV4089株，经PCR和酶切鉴定，构建成携带猪链球菌溶血素基因的重组减毒鼠伤寒沙门氏菌。结果表明：该减毒株具有相对安全性；用酶切和PCR鉴定法证实在无抗生素存在的条件下携带重组质粒的减毒株比较稳定；SDS-PAGE显示SLY能在宿主菌中进行表达。该结果为进一步研究制备猪链球菌口服活疫苗奠定了基础。  相似文献   

Cytocompatibiltiy of degradable calcium metaphosphate with human marrow mesenchymal stem cells     

QIU Chui-yuan  TANG Wen-jie  DAI Yun  WU Yue-heng  SUN Fen-yong  CHEN Qiong-yu  LI Ling-song△  HONG An 《园艺学报》2005,21(7):1378-1383

AIM: To screen the cytotoxicity of degradable calcium metaphosphate (dCMP) compared with hydroxyapatite (HA). The proliferation and differentiation abilities of human marrow mesenchymal stem cells (MSC) were used to exhibit the cytotoxicity. METHODS: The cell morphology of MSC was analysed after direct contact with dCMP at different time points by scanning electron microscopy analysis. The degradation products of dCMP and HA were analysed with inductively coupled plasma torch and ion chromatography. The cytotoxic effect of degradation products of dCMP was evaluated by FACS, quantitative assay of ALP and ARS, respectively. RESULTS: dCMP enhanced the proliferation of MSC, but didn't interfere the osteogenic differentiation process of MSC and its mineralization. HA inhibited the proliferation of MSC and the mineralization of osteogenic differentiated MSC, while it did not interfere the osteogenic differentiation process of MSC. CONCLUSION: dCMP had a better cytocompatibility with MSC than HA, which might allow for its use as skeleton scaffolds.  相似文献   

玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定     

TIAN Shi-Ke  QIN Xin-Er  ZHANG Wen-Liang  DONG Xue  DAI Ming-Qiu  YUE Bing 《作物学报》1962,46(12):1991-1996

Maize is one of the best crops in the utilization of heterosis. Male sterile lines are important germplasms for the hybrids production. A male sterile mutant named mi-ms-3 was obtained by screening in a mutator insertion library. The number of male anthers in tassel decreased and not exserted. There were few anthers with only two pollen sacs in the mutant tassels, and some of the anthers were degenerated to membranous and formed filaments at their ends. Although pollens in the anthers could be stained by I₂-KI, pollen shedding was abnormal and the number of pollen grains decreased. The number of silks in the ear of the mutant increased, and there was a sterile grain on both sides of the maturated kernel. Fertility of F₁ plants, which were obtained by hybridization between mi-ms-3 and maize inbred Mo17, was normal. Genetic analysis of F₂ population showed that the mutant phenotype was controlled by a recessive gene. The candidate gene was preliminarily mapped on the long arm of chromosome 3 by BSA and it was located between a SSR marker and an Indel marker with a distance of 1.5 cM. There are 21 candidate genes in this region. It was finally found that the insertion mutation of Mu transposon occurred at 30 bp upstream of the coding region of zm00001d042618 (zmm16) by transponson tagging and sequencing analysis. The results showed that mi-ms-3 was a new allele of sts1, which caused by a single base mutation in the coding region. RT-PCR analysis indicated that the expression of zmm16 in the mutant was decreased. The identification of the new allelic mutant of sts1 in this study would provide new materials for the study of flower development and hybrid seed production.  相似文献   

温度和湿度对伴性赤蚁sch系统致死性的影响   总被引：1，自引：1，他引：0  

卢忠燕  夏庆友  代方银  梁应霞 《蚕业科学》2003,29(2):125-130

为进一步探讨家蚕伴性赤蚁(sch)致死的机理,比较了不同温度、湿度催青条件下,sch系统及其杂交F_1代和对照种黑蚁系统的孵化率、失水率、含水率、失水临界温度等。结果表明:伴性赤蚁sch系统在已_3至已_4期对温湿度敏感,受到高温和干燥的冲击,将获得“孵化不能”性状;温度、湿度因子对赤蚁sch孵化抑制均有较大的独立作用,sch表现出一定的“温度敏感性”和“湿度敏感性”;温度、湿度都能通过影响胚胎体内水分平衡失调而引起这种孵化不能;在不引起水分变化的情况下,温度还存在一条独立的抑制赤蚁孵化的途径。  相似文献   

茶秆竹高生长模型的比较及组合选择   总被引：2，自引：1，他引：1  

代全林  陈存及  肖书平  陈东阳  董建文 《福建林学院学报》2002,22(2):129-132

通过对 5种 S增长模型的比较及组合选择 ,结果表明 :S增长模型都具有单调性、渐近性和拐点 ,此特征能客观地反映诸多自然和经济规律 .通过一定的加权组合 ,用多个增长模型来反映 S变化过程 ,可以提高拟合的精度 ,多个模型的组合要优于单个模型 .用加权模型可以很好地反映茶秆竹的高生长过程 ,在实际生产中可选择 yp2 或 yp 3 组合  相似文献   

我国草原现状及其生态恢复途径初探     

王银柱  戴良先  刘晓英  刘刚  武高林 《四川草原》2007,(5)

介绍了我国草原所面临的一些主要问题,如退化、沙漠化、水土流失等,分析了其主要原因,然后针对我国草原退化和破坏的具体情况相应地提出了一些生态恢复措施和方法建议。  相似文献   

新扬州鸡早期增重的OPAY02-SCAR分子标记研究     

戴国俊  谢恺舟  王志跃  吴圣龙  施会强  Olowofeso O  盛浩伟  王金玉 《畜牧兽医学报》2006,37(6):544-549

OPAY02型2条多态性条带经克隆、测序和引物设计后,转换成SCAR标记,并对86个新扬州鸡随机交配后代基因组DNA进行了PCR扩增.2条带DNA序列与红色原鸡基因组序列比对结果表明,大分子量条带与位于红色原鸡第3号染色体上序列有98%的同源性,共检测到8个SNPS,其中195位的碱基T→G,316位的A→T,538位的G→A,731位的T→A,1 147位的G→A,1 329位的T→C,1 927位的C→T,2 081位的C→T,小分子量条带与红色原鸡没有同源序列,推测新扬州鸡野祖除红色原鸡外,还有其它来源.SCAR标记分析表明,经条件优化随机扩增的OPAY02型标记稳定、可靠,可用于遗传分析.2条带所在座位群体基因型平衡性测验结果表明,所测新扬州鸡群体处于平衡状态,选择可以打破平衡,有利于动物育种.  相似文献