Heavy Metal Accumulation in Different Rice Cultivars as Influenced by Foliar Application of Nano-silicon     

Shihua Wang  Fayuan Wang  Shuangcheng Gao  Xugang Wang 《Water, air, and soil pollution》2016,227(7):228

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基于TRIZ的方草捆收集车集捆装置设计     

郝兴玉  张宁  戴晓军  高磊  董佳佳 《农业工程》2016,6(2):79-83

针对方草捆收集车集捆装置工作稳定性较差的问题，利用TRIZ系统分析方法及ProInnovator软件对集捆装置进行分析，提出了改进方案，对改进方案进行综合评价，得到了最优方案。   相似文献   

Effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and antioxidant capacity     

Yang Zi  Chi Ma  Huimin Li  Suting Shen  Yingchun Liu  Ming Li  Feng Gao 《Animal Science Journal》2021,92(1):e13613

This study investigated the effects of intrauterine growth restriction during late pregnancy on the ovine fetal renal function and renal antioxidant capacity. Eighteen ewes pregnant were randomly divided into control group (CG, ad libitum, 0.67 MJ ME·BW^−0.75·day⁻¹, n = 6), restricted group 1 (RG1, 0.18 MJ ME·BW^−0.75·day⁻¹, n = 6), and restricted group 2 (RG2, 0.33 MJ ME·BW^−0.75·day⁻¹, n = 6). At 140 days, the fetal blood, allantoic fluid and kidney tissue were collected to determinate fetal renal function and renal antioxidant capacity. The results showed that the fetal weight, kidney weight, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), aquaporin-2 (AQP-2) and aquaporin-3 (AQP-3), and total antioxidant capacity (T-AOC) in RG1 group were decreased compared with the CG (P < 0.05), but the contents of β2-Microglobulin (β 2-MG), cystatin C (Cys-C), filtered sodium excretion fraction (FENa), malondialdehyde (MDA), and hydroxyl radical (OH) in RG1 group were increased (P < 0.05). The impaired ovine fetal renal growth, antioxidant imbalance and dysfunction of glomerulus ultrafiltration, and the renal tubules reabsorption were induced by maternal malnutrition during late pregnancy.  相似文献   

诱导Paracin1.7生成的刺激因子的分离与纯化     

田甜  孙艳阳  康杰  宋刚  高冬妮  葛菁萍 《中国农学通报》2020,36(32):17-22

旨在分离纯化枯草芽孢杆菌(Bacillus subtilis)中能够诱导细菌素Paracin1.7分泌的刺激因子,并探明其对副干酪乳杆菌(Lactobacillus paracasei) HD1.7细菌素生成量及群体感应相关基因luxS表达的影响。本研究通过60%硫酸铵沉淀、CM Sepharose Fast Flow弱阳离子交换柱层析中度纯化及Superdex 75凝胶层析精度纯化,将获得的层析分离物与L. paracasei HD1.7共培养,检测共培养发酵液的抑菌活性及luxS基因的转录水平,并用SDS-PAGE与Native-PAGE检测分离物质。结果表明L. paracasei HD1.7抑菌活性为126.68%;luxS基因上调表达,为对照菌株的2.43倍;刺激因子的表观分子量约为30 kDa。本研究利用三步法初步分离纯化出诱导Paracin1.7生成的刺激因子,明确该刺激因子可以启动种间群体感应相关基因luxS,为L. paracasei HD1.7的群体感应研究奠定了理论和技术基础。  相似文献   

新疆多浪羊FecB突变检测及与产羔数的关系   总被引：2，自引：0，他引：2  

史洪才  高志英  牛志刚  白杰  冯利君  李红波  贾斌  张扬 《农业生物技术学报》2011,19(2)

骨形态发生蛋白受体IB基因(BMPR-IB)FecB突变是影响布鲁拉美利奴和小尾寒羊高繁殖力的主效基因.本研究采集产双羔及以上的多浪羊(Ovis aries)母羊135只及后代羔羊血样共353份,公羊血样211份,利用PCR-RFLP技术检测FecB突变,并分析不同基因型个体母羊的产羔数.结果表明:在多浪羊群体中检测到BB、B+和++基因型,基因型频率分别为0.005,0.146和0.849.B+型多浪羊产羔数均值比++型多0.51只(P<0.01),BB型多浪羊产羔数分别与B+和++型差异不显著.多浪羊群体在FecB位点上处于Hard-Weinberg平衡状态,表明在该位点上没有受到选择的影响.本研究首次在新疆多浪羊群体中发现了FecB突变BB基因型公、母羊个体(1只公羊和2只母羊),揭示多浪羊多羔产生的机理可能与布鲁拉美利奴和小尾寒羊相同,在生产过程中可以根据携带FecB突变的情况开展选种选配,组建多浪羊肉用高繁品系.  相似文献   

雪山草鸡感染传染性法氏囊病病毒新型变异株的鉴定     

姜楠  王雨龙  张文英  牛鑫鑫  刘长军  高玉龙  高立  崔红玉  李凯  潘青  张艳萍  刘爱晶  王笑梅  祁小乐 《中国动物检疫》2021,38(5):119-125

传染性法氏囊病病毒(infectious bursal disease virus,IBDV)新型变异株正在我国广泛蔓延,给商品肉鸡群和蛋鸡群带来了新的威胁。本研究对一个疑似发生非典型传染性法氏囊病的地方品种雪山草鸡群进行了RT-PCR检测,并对分离的3株毒株进行了基因测序及序列分析。结果显示,引起该鸡群发病的病原是IBDV新型变异株,属于A2dB1基因型;这3个毒株的VP2高变区编码蛋白上含有IBDV变异株的特征性氨基酸,也具有IBDV新型变异株的独特氨基酸。结果提示,我国地方品种鸡群中也有IBDV新型变异株的流行,现有的部分商品化IBDV疫苗已不能有效预防IBDV新型变异株的流行。  相似文献   

苹小卷叶蛾在6个苹果品种上的发育与繁殖     

孙丽娜  闫文涛  张怀江  仇贵生 《山东农业科学》2015,(1):92-95,104

为探索不同品种苹果对苹小卷叶蛾生长发育和繁殖的影响,在温度(26±1)℃、相对湿度(80±5)%、光周期L∶D=16 h∶8 h的室内条件下,测定了苹小卷叶蛾在小国光、大国光、红玉、祝光、红星和金帅上的发育历期、存活率和产卵量,并组建了其各自的生命周期表。结果显示,苹小卷叶蛾在6个品种上的发育历期差异显著,全世代发育历期以小国光最短(33.95 d),以红玉最长(39.76 d);平均单雌产卵量以红玉最高(107.24粒),小国光次之(85.00粒);而存活率则无显著差异。生命表参数中净生殖率(R0)、内禀增长率(rm)和周限增长率(λ)均以小国光最高,世代平均周期(T)和种群加倍时间(Dt)以小国光最短,表明6种试验品种中小国光是苹小卷叶蛾最适宜的寄主品种。  相似文献   

Summer drought decreases Leymus chinensis productivity through constraining the bud,tiller and shoot production     

Junfeng Wang  Yujie Shi  Yunna Ao  Dafu Yu  Jiao Wang  Song Gao  Johannes M. H. Knops  Chunsheng Mu  Zhijian Li 《Journal of Agronomy and Crop Science》2019,205(6):554-561

Extreme drought events can directly decrease productivity in perennial grasslands. However, for rhizomatous perennial grasses it remains unknown how drought events influence the belowground bud bank which determines future productivity. Ninety‐day‐long drought events imposed on Leymus chinensis, a rhizomatous perennial grass, caused a 41% decrease in the aboveground biomass and a 28% decrease in belowground biomass. Aboveground biomass decreased due to decrease in both the parent and the daughter shoot biomass. The decreases in daughter shoot biomass were due to reductions in both the shoot number and each individual shoot weight. Most importantly, drought decreased the bud bank density by 56%. In addition, drought induced a bud allocation change that decreased by 41% the proportion of buds that developed into shoots and a 41% increase in the buds that developed into rhizomes. Above results were supported by our field experiment with watering treatments. Thus, a 90‐day‐long summer drought event decreases not only current productivity but also future productivity, because the drought reduces the absolute bud number. However, plasticity in plant development does partly compensate for this reduction in bud number by increasing bud development into rhizomes, which increases the relative allocation of buds into future shoots, at the cost of a decrease in current shoots.  相似文献   

优质高产棉花新品种鲁棉1157的选育及栽培技术     

王家宝  高明伟  张超  姜辉  陈莹  王秀丽  柴启超  王永翠  张卓  房健  赵军胜 《中国棉花》2020,47(9):23-24

介绍了鲁棉1157 的选育过程、农艺特征特性、产量、品质、抗性及栽培技术要点。  相似文献   

化学发光免疫分析技术在动物疫病检测中的应用     

赵协  安利民  高沙沙  张玉  乔飞 《中国动物检疫》2020,37(8):82-87

化学发光免疫分析(chemiluminesecence immunoassay,CLIA)是用于疫病检测的一种免疫检测技术,是基于免疫反应原理,结合化学发光检测技术而建立的。与放射免疫分析、酶免疫分析等标记免疫技术相比,具有无放射性污染、灵敏度高和特异性强的特点,已被广泛应用于疫病流行病学调查、诊断、药物分析以及微生物检测等方面。本文简要介绍了CLIA技术的基本原理及发展,从细菌、病毒和寄生虫检测方面,综述了近年来该技术在动物疫病检测中的应用,提出该技术正向高特异性、高灵敏度、高通量、快速和自动化检测的方向发展,这为今后CLIA在动物疫病检测中的应用研究提供参考。  相似文献