Immunohistochemical detection of pituitary transcription factor-1 in paraffin sections of bovine adenohypophysis after wet heat-induced antigen retrieval     

Masatoshi MATSUZAKI  Takahiro SATO  Kouichi WATANABE  Nobuya SHIBA  Eisaku TSUNEISHI  Takahiro YAMAGUCHI 《Animal Science Journal》2003,74(2):137-143

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The pain-relieving effects of lactoferrin on oxaliplatin-induced neuropathic pain     

Takeshi FUJIMURA  Aiko IGUCHI  Atsushi SATO  Shinji KAGAYA  Tatsuo HOSHINO  Takashi TAKEUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2020,82(11):1648

Oxaliplatin (OXL) therapy often causes side effects including chronic peripheral neuropathy. We investigated the pain-relieving effects of recombinant human lactoferrin (rhLf) as well as a long-acting IgG-Fc fused rhLf (rhLf-Fc) on OXL-induced neuropathic pain. We used the hLf in this study, because the homology between mouse Lf and hLf is higher than that of bovine Lf. In addition, rhLf-Fc is expected to enhance the analgesic effect due to the life extension effect in the body. We administered OXL (2 mg/kg, i.v.) to mice twice weekly for 4 weeks. Phosphate buffered saline (PBS), rhLf (100 mg/kg, i.p.) or rhLf-Fc (100 mg/kg, i.p.) was administered once a week from day 15 to 32. We also assessed the continuous infusion of same drugs (10 mg/kg/day) into the external jugular vein by using an osmotic pump. Both of rhLf and rhLf-Fc significantly reduced the hypersensitivity to mechanical stimulation when they were administered intraperitoneally. The continuous infusion of rhLf resulted in a more pronounced effect. Histopathological analysis of sciatic nerve showed that both rhLf and rhLf-Fc tended to reduce nerve fiber damage, but no significant difference was observed in nerve fiber cross-sectional area. Therefore, it was suggested that rhLf or rhLf-Fc injection could be an option for controlling neuropathic pain, which are side effects of OXL.  相似文献   

Antimicrobial resistance profiles and phylogenetic groups of Escherichia coli isolated from healthy Thoroughbred racehorses in Japan     

Wataru SATO  Eddy SUKMAWINATA  Ryoko UEMURA  Takuya KANDA  Kanichi KUSANO  Yoshinori KAMBAYASHI  Takashi SATO  Yuhiro ISHIKAWA  Ryohei TOYA  Masuo SUEYOSHI 《Journal of Equine Science》2020,31(4):85

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Gene expression and hormonal regulation of adiponectin and its receptors in bovine mammary gland and mammary epithelial cells     

Yoshihisa OHTANI  Tomo YONEZAWA  Sang‐Houn SONG  Tatsuyuki TAKAHASHI  Astrid ARDIYANTI  Katsuyoshi SATO  Akihiko HAGINO  Sang‐gun ROH  Kazuo KATOH 《Animal Science Journal》2011,82(1):99-106

Although the functions of adiponectin, a differentiated adipocyte‐derived hormone, in regulating glucose and fatty acid metabolism are regulated by two subtypes of adiponectin receptors (AdipoRs; AdipoR1 and AdipoR2), those in ruminants remain unclear. Therefore we examined the messenger RNA (mRNA) expression levels of adiponectin and its receptors in various bovine tissues and mammary glands among different lactation stages, and the effects of lactogenic hormones (insulin, dexamethasone and prolactin) and growth hormone (GH) on mRNA expression of the AdipoRs in cultured bovine mammary epithelial cells (BMEC). AdipoRs mRNAs were widely expressed in various bovine tissues, but adiponectin mRNA expression was significantly higher in adipose tissue than in other tissues. In the mammary gland, although adiponectin mRNA expression was significantly decreased at lactation, AdipoR1 mRNA expression was significantly higher at peak lactation than at the dry‐off stage. In BMEC, lactogenic hormones and GH upregulated AdipoR2 mRNA expression but did not change that of AdipoR1. In conclusion, adiponectin and its receptor mRNA were expressed in various bovine tissues and the adiponectin mRNA level was decreased during lactation. These results suggest that adiponectin and its receptors ware changed in mammary glands by lactation and that AdipoRs mRNA expression was regulated by different pathways in BMEC.  相似文献   

Rupture of the Gastrocnemius Muscle in Neonatal Thoroughbred Foals: A Report of Three Cases     

Fumio SATO  Ryo SHIBATA  Mitsumori SHIKICHI  Katsumi ITO  Harutaka MURASE  Takanori UENO  Hidehumi FURUOKA  Kazutaka YAMADA 《Journal of Equine Science》2014,25(3):61-64

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Time course of changes in antioxidant activity of milk from dairy cows fed a trehalose‐supplemented diet     

Naoto AOKI  Kan SATO  Shuhei KANDA  Kazuhisa MUKAI  Yoshiaki OBARA  Hisao ITABASHI 《Animal Science Journal》2013,84(1):42-47

This study was to investigate the time course of changes to the antioxidant activity of milk from cows fed a trehalose‐supplemented diet, and to determine possible underlying mechanisms for observed changes. Six Holstein cows were used, and subjected to two experimental feeding periods consisting of a 1% trehalose‐supplemented diet for 10 days, followed by a basal diet only (no trehalose) for 10 days. 1,1‐Diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging activities in milk were gradually increased during the trehalose supplementation period and were highest at the end of the second period. However, trehalose was not detected in the milk and plasma of dairy cows fed a diet supplemented with trehalose for 10 days, indicating that the increased antioxidant activity in the milk of trehalose‐fed cows is not due to the direct transfer of trehalose to the milk. Plasma DPPH activities exhibited a similar time course to that seen for milk. Relative superoxide dismutase (SOD) activities in the rumen were higher 3 days after the end of trehalose supplementation than at any other time during the experimental periods. These results suggested that the improved antioxidant activity in milk and plasma of cows fed a trehalose‐supplemented diet was due to improved ruminal relative SOD activity.  相似文献   

Effects of trichostatin A on in vitro development and transgene function in somatic cell nuclear transfer embryos derived from transgenic Clawn miniature pig cells     

Takehiro HIMAKI  Taka‐aki YOKOMINE  Masahiro SATO  Sonshin TAKAO  Kazuchika MIYOSHI  Mitsutoshi YOSHIDA 《Animal Science Journal》2010,81(5):558-563

The present study was carried out to examine the effects of post‐activation treatment of trichostatin A (TSA), a histone deacetylase inhibitor, on in vitro development and transgene function of somatic cell nuclear transfer (SCNT) embryos derived from Clawn miniature pig embryonic fibroblast (PEF) transfected with a bacterial endo‐β‐galactosidase C gene (removal of the α‐galactosyl (Gal) epitope). SCNT embryos were incubated with or without TSA (50 or 100 nmol/L) after activation, cultured in vitro and assessed for cleavage, blastocyst formation and transgene function. The rate of blastocyst formation was significantly higher in SCNT embryos treated with 50 nmol/L TSA than that in control (P < 0.05), whereas the rate of cleavage and cell number of blastocyst did not differ. Following labelling with fluorescein isothiocyanate‐labelled BS‐I‐B₄ isolectin, the intensity of fluorescence observed on cell‐surface was dramatically reduced in transgenic SCNT blastocyst in comparison with non‐transgenic SCNT blastocyst. However, the reduction of α‐Gal epitope expression in transgenic SCNT blastocyst was not affected by TSA treatment. The results of this study showed that post‐activation treatment with 50 nmol/L TSA is effective to improve in vitro developmental capacity of transgenic SCNT miniature pig embryos without the modification of transgene function.  相似文献   

Nucleotide polymorphisms and the 5′‐UTR transcriptional analysis of the bovine growth hormone secretagogue receptor 1a (GHSR1a) gene     

Masanori KOMATSU  Yuki FUJIMORI  Yoichi SATO  Hiroaki OKAMURA  Seiki SASAKI  Tomohito ITOH  Mitsuo MORITA  Ryoichi NAKAMURA  Toshiaki OE  Masako FURUTA  Junpei YASUDA  Takatoshi KOJIMA  Toshio WATANABE  Takeshi HAYASHI  Aduli E.O. MALAU‐ADULI  Hideaki TAKAHASHI 《Animal Science Journal》2010,81(5):530-550

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Mobility of the forearm skeleton in the Asiatic black (Ursus thibetanus), brown (U. arctos) and polar (U. maritimus) bears     

Hayato AMAIKE  Motoki SASAKI  Nao TSUZUKI  Mitsunori KAYANO  Motoharu OISHI  Kazutaka YAMADA  Hideki ENDO  Tomoko ANEZAKI  Naoya MATSUMOTO  Rumiko NAKASHITA  Misako KUROE  Hajime TARU  Gen BANDO  Yuko IKETANI  Ryohei NAKAMURA  Nobutaka SATO  Daisuke FUKUI  Nobuo KITAMURA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1284

In several primates and carnivores, pronation/supination angles of the forearm skeleton were examined, and it is thought that a larger angle is useful to acquire dexterous behaviors in feeding and/or life style, including climbing. In this study, the pronation/supination angles in Asiatic black, brown and polar bears were nondestructively examined. These specimens were classified as adult or non-adult. Three or four carcasses of each group of Asiatic black and brown bears were used for CT analysis, whereas only one adult polar bear was used. The forearms were positioned within the gantry of a CT scanner in both maximally supinated and pronated states. Extracted cross-sectional CT images of two positions were superimposed by overlapping the outlines of each ulna. The centroids of the radii were detected, and then the centroid of each radius and the midpoint of a line which connects between both ends of the surface of each radius facing the ulna, were connected by lines to measure the angle of rotation as an index of pronation/supination. In adult brown and polar bears, the angles were smaller as compared with the other groups (Asiatic black and non-adult brown bears). Asiatic black and non-adult brown bears can climb trees, whereas adult brown bears and polar bears cannot. This suggests that the pronation/supination angle is related to arboreal activity in Ursidae.  相似文献   

Time‐coordinated prevalence of extracellular HGF,FGF2 and TGF‐β3 in crush‐injured skeletal muscle     

Mai‐Khoi Q. DO  Takahiro SUZUKI  Borjigin GERELT  Yusuke SATO  Wataru MIZUNOYA  Mako NAKAMURA  Yoshihide IKEUCHI  Judy E. ANDERSON  Ryuichi TATSUMI 《Animal Science Journal》2012,83(10):712-717

Successful regeneration and remodeling of neuromuscular junctions are critical for restoring functional capacities and properties of skeletal muscle after damage, and axon‐guidance molecules may be involved in the signaling that regulates such restoration. Recently, we found that early‐differentiated satellite cells up‐regulate a secreted neural chemorepellent Sema3A upon in vivo muscle‐crush injury. The study also revealed that Sema3A expression is up‐regulated in primary satellite‐cell cultures in response to hepatocyte growth factor (HGF) and basic fibroblast growth factor (FGF2) and is prevented by transforming growth factor (TGF)‐β2, 3. In order to verify the physiological significance of this regulation in vitro, the present study was designed to estimate the time‐course of extracellular HGF, FGF2 and TGF‐β3 concentrations after crush‐injury of Gastrocnemius muscle in the rat lower hind‐limb, using a combination of a non‐homogenization/non‐spin extraction of extracellular wound fluids and enhanced chemiluminescence–Western blotting analyses. Results clearly demonstrated that active HGF and FGF2 are prevalent in 2–8 days post‐crush, whereas active TGF‐β3 increases after 12 days, providing a better understanding of the time‐coordinated levels of HGF, FGF2 and TGF‐β3 that drive regulation of Sema3A expression during regenerative intramuscular moto‐neuritogenesis.  相似文献