全文获取类型
| 收费全文 | 6056篇 |
| 免费 | 297篇 |
| 国内免费 | 4篇 |
专业分类
| 林业 | 501篇 |
| 农学 | 183篇 |
| 基础科学 | 35篇 |
| 1106篇 | |
| 综合类 | 903篇 |
| 农作物 | 228篇 |
| 水产渔业 | 343篇 |
| 畜牧兽医 | 2478篇 |
| 园艺 | 177篇 |
| 植物保护 | 403篇 |
出版年
| 2023年 | 33篇 |
| 2022年 | 48篇 |
| 2021年 | 84篇 |
| 2020年 | 143篇 |
| 2019年 | 128篇 |
| 2018年 | 143篇 |
| 2017年 | 132篇 |
| 2016年 | 133篇 |
| 2015年 | 124篇 |
| 2014年 | 155篇 |
| 2013年 | 293篇 |
| 2012年 | 307篇 |
| 2011年 | 397篇 |
| 2010年 | 229篇 |
| 2009年 | 187篇 |
| 2008年 | 321篇 |
| 2007年 | 333篇 |
| 2006年 | 305篇 |
| 2005年 | 312篇 |
| 2004年 | 291篇 |
| 2003年 | 290篇 |
| 2002年 | 269篇 |
| 2001年 | 86篇 |
| 2000年 | 87篇 |
| 1999年 | 92篇 |
| 1998年 | 60篇 |
| 1997年 | 62篇 |
| 1996年 | 59篇 |
| 1995年 | 47篇 |
| 1994年 | 56篇 |
| 1993年 | 64篇 |
| 1992年 | 55篇 |
| 1991年 | 54篇 |
| 1990年 | 58篇 |
| 1989年 | 51篇 |
| 1988年 | 47篇 |
| 1987年 | 48篇 |
| 1986年 | 62篇 |
| 1985年 | 39篇 |
| 1984年 | 55篇 |
| 1983年 | 37篇 |
| 1982年 | 45篇 |
| 1981年 | 37篇 |
| 1980年 | 28篇 |
| 1979年 | 28篇 |
| 1978年 | 30篇 |
| 1977年 | 34篇 |
| 1974年 | 23篇 |
| 1973年 | 37篇 |
| 1972年 | 26篇 |
排序方式： 共有6357条查询结果，搜索用时 15 毫秒
101.
单克隆抗体夹心ELISA试验检测鸡新城疫病毒抗原的研究 总被引：1，自引：0，他引：1
从10株抗鸡新城疫病毒(NDV)特异性单克隆抗体(MAbs) 中筛选出2株MAbs—M22和F23,分别用作包被抗体和酶标抗体而建立的MAbs—夹心ELISA试验,对提纯NDV的最小检出量为25ng/ml病毒蛋白.NDV鸡阳性血清对本试验的特异性阻断作用和与其他禽类病毒交叉反应的阴性结果,证明本试验对NDV的特异性。从临床疑为鸡新城疫的724只病鸡的口腔棉试样品中共检出阳性502只,其中200个样品与病毒分离试验结果完全一致,两者均查出阳性鸡176只。 相似文献
102.
Sabine Augustin Jan Evers Hans-Peter Dietrich Johannes Eichhorn Thomas Haussmann Regina Icke Ansgar Isenberg Wolfgang Lux Monica Musio Hans Pretzsch Winfried Riek Thomas Rötzer Bernd Schultze Andreas Schulze Jörg Schröder Walter Seidling Nicole Wellbrock Klaus von Wilpert Barbara Wolff 《European Journal of Forest Research》2005,124(4):251-260
In the 1970s unexpected forest damages, called “new type of forest damage” or “forest decline”, were observed in Germany and
other European countries. The Federal Republic of Germany and the German Federal States implemented a forest monitoring system
in the early 1980s, in order to monitor and assess the forest condition. Due to the growing public awareness of possible adverse
effects of air pollution on forests, in 1985 the ICP Forests was launched under the convention on long-range transboundary
air pollution (CLRTAP) of the United Nations Economic Commission for Europe (UN-ECE). The German experience in forest monitoring
was a base for the implementation of the European monitoring system. In 2001 the interdisciplinary case study “concept and
feasibility study for the integrated evaluation of environmental monitoring data in forests”, funded by the German Federal
Ministry of Education and Research, concentrated on in-depths evaluations of the German data of forest monitoring. The objectives
of the study were: (a) a reliable assessment of the vitality and functioning of forest ecosystems, (b) the identification
and quantification of factors influencing forest vitality, and (c) the clarification of cause-effect-relationships leading
to leaf/needle loss. For these purposes additional data from external sources were acquired: climate and deposition, for selected
level I plots tree growth data, as well as data on groundwater quality. The results show that in particular time series analysis
(crown condition, tree growth, and tree ring analysis), in combination with climate and deposition are valuable and informative,
as well as integrated evaluation of soil, tree nutrition and crown condition data. Methods to combine information from the
extensive and the intensive monitoring, and to transfer process information to the large scale should be elaborated in future.
相似文献
| Sabine AugustinEmail: |
103.
Effects of N, NP, and NPKS fertilizers applied to four-year old hybrid poplar plantations 总被引：1，自引：0，他引：1
Mineral fertilizers were applied to adjacent plantations of 2, 4-year old, hybrid poplars: clone 27 (Northwest, P. deltoides × P. balsamifera) and 794 (Brooks, P. deltoides × P. × petrowskyana), in north eastern Alberta. Fertilization was done in May 2003 to see whether growth rates could be increased and rotations
shortened. Three fertilizers (N, NP and NPKS + Cu + Zn) were applied at each of three rates (supplying N at 0, 100 and 200 kg ha−1) in a factorial randomized block design to the two separate plantations. Fertilization with 200 kg ha−1 N and 100 kg ha−1 P increased volume growth of clone 27 by 1 m3 ha−1 year−1 over 2 years. Clone 794 showed no volume response to fertilization, but produced 8.7 m3 ha−1 more than clone 27. Leaf area, dry mass and nutrient concentrations of both clones increased in the first year after fertilization,
showing that fertilizer uptake occurred. Decrease in leaf size between 2003 and 2004 was affected by fertilizer level in clone
794. There were differences between some nutrient concentrations in the soils occupied by the two clones, and clone 794 had
higher leaf concentrations of N, K, Ca, S, Mn, Zn, B and Mo than clone 27. Fertilization of 4-year old plantations of either
clone was unwarranted, and planting clone 794 would be likely to provide greater yield than planting and fertilizing clone
27. 相似文献
104.
105.
Roger D Magarey Sarah SH Klammer Thomas M Chappell Christina M Trexler Godshen R Pallipparambil Ernie F Hain 《Pest management science》2019,75(12):3129-3134
Agricultural industrialization and the subsequent reliance on pesticides has resulted in numerous unintended consequences, such as impacts upon the environment and by extension human health. Eco‐efficiency is a strategy for sustainably increasing production, while simultaneously decreasing these externalities on ecological systems. Eco‐efficiency is defined as the ratio of production to environmental impacts. It has been widely adopted to improve chemical production, but we investigate the challenges of applying eco‐efficiency to pesticide use. Eco‐efficiency strategies include technological innovation, investment in research and development, improvement of business processes, and accounting for market forces. These components are often part of integrated pest management (IPM) systems that include alternatives to pesticides, but its implementation is often thwarted by commercial realities and technical challenges. We propose the creation and adoption of an eco‐efficiency index for pesticide use so that the broad benefits of eco‐efficient strategies such as IPM can be more readily quantified. We propose an index based upon the ratio of crop yield to a risk quotient (RQ) calculated from pesticide toxicity. Eco‐efficiency is an operational basis for optimizing pest management for sustainability. It naturally favors adoption of IPM and should be considered by regulators, researchers, and practitioners involved in pest management. © 2019 Society of Chemical Industry 相似文献
106.
Waseem Akbar Anilkumar Gowda Jeffrey E Ahrens Jason W Stelzer Robert S Brown Scott L Bollman John T Greenplate Jeffrey Gore Angus L Catchot Gus Lorenz Scott D Stewart David L Kerns Jeremy K Greene Michael D Toews David A Herbert Dominic D Reisig Gregory A Sword Peter C Ellsworth Larry D Godfrey Thomas L Clark 《Pest management science》2019,75(3):867-877
107.
108.
Corné Kempenaar Thomas Been Johan Booij Frits van Evert Jean-Marie Michielsen Corné Kocks 《Potato Research》2017,60(3-4):295-305
Precision agriculture is a farming management concept based on observing, measuring and responding to inter- and intra-field variability in crops. In this paper, we focus on responding to intra-field variability in potato crops and analyse variable rate applications (VRAs). We made an overview of potential VRAs in potato crop management in The Netherlands. We identified 13 potential VRAs in potato, ranging from soil tillage to planting to crop care to selective harvest. We ranked them on availability of ‘proof of concept’ and on-farm test results. For five VRAs, we found test results allowing to make a cost-benefit assessment. These five VRAs were as follows: planting, soil herbicide weed control, N side dress, late blight control and haulm killing. They use one of two types of spatial data: soil maps or biomass index maps. Data on costs and savings of the VRAs showed that the investments in VRAs will pay off under practical conditions in The Netherlands. Savings on pesticide use and N-fertilizer use with the VRAs were on average about 25%, which benefits the environment too. We foresee a slow but gradual adoption of VRAs in potato production. More VRAs will become available given ongoing R&D. The perspectives of VRAs in potatoes are discussed. 相似文献
109.
Monica L. Bond Curtis M. Bradley Christian Kiffner Thomas A. Morrison Derek E. Lee 《Landscape Ecology》2017,32(8):1705-1721
Context
Managers are faced with numerous methods for delineating wildlife movement corridors, and often must make decisions with limited data. Delineated corridors should be robust to different data and models.Objectives
We present a multi-method approach for delineating and validating wildlife corridors using multiple data sources, which can be used conserve landscape connectivity. We used this approach to delineate and validate migration corridors for wildebeest (Connochaetes taurinus) in the Tarangire Ecosystem of northern Tanzania.Methods
We used two types of locational data (distance sampling detections and GPS collar locations), and three modeling methods (negative binomial regression, logistic regression, and Maxent), to generate resource selection functions (RSFs) and define resistance surfaces. We compared two corridor detection algorithms (cost-distance and circuit theory), to delineate corridors. We validated corridors by comparing random and wildebeest locations that fell within corridors, and cross-validated by data type.Results
Both data types produced similar RSFs. Wildebeest consistently selected migration habitat in flatter terrain farther from human settlements. Validation indicated three of the combinations of data type, modeling, and corridor detection algorithms (detection data with Maxent modeling, GPS collar data with logistic regression modeling, and GPS collar data with Maxent modeling, all using cost-distance) far outperformed the other seven. We merged the predictive corridors from these three data-method combinations to reveal habitat with highest probability of use.Conclusions
The use of multiple methods ensures that planning is able to prioritize conservation of migration corridors based on all available information.110.
Pedro Martínez-Gómez Angela S. Prudencio Thomas M. Gradziel Federico Dicenta 《Euphytica》2017,213(8):197
Regulation of flowering time in almond, as in other Prunus species, is a complex process involving both chill and heat requirements. Following exposure to appropriate consecutive periods of cold and warm temperatures, the buds break dormancy and sprout or flower depending on bud type. To maximize flowering and subsequent vegetative growth and fruit set, chilling and ensuing warm temperature requirements have to be fully satisfied. Because of its potential for very early flowering, flowering time in almond is a major determinant of its adaptation to new environments. In colder regions, Late-flowering is often necessary to avoid frost damage during and just after flowering. Consequently, the selection of delayed flowering times remains an important objective in almond improvement programs. Flowering time is considered a quantitative though highly heritable trait. In addition, a dominant gene (Late flowering, Lb), originally identified in a spontaneous mutation of the Californian almond cultivar ‘Nonpareil’, was also described. The objective of this review is a comparative analysis of the effects of regional adaptation, breeding and mutation on the delay of flowering time in new almond cultivars. Findings indicate that the adaptation of almonds from the Mediterranean basin to colder regions in Northern Europe and America has been mainly achieved through delayed flowering. These adapted late-flowering cultivars have usually been developed by selecting desired quantitative genes within each regional germplasm. Additional progress thus appears achievable with a more comprehensive understanding of the quantitative and qualitative genetics controlling this trait. The use of molecular markers for the early selection of genes conferring late flowering, including both spontaneous mutations as well as unique regional germplasm, should allow development of even later cultivars including ultra-late cultivars flowering as into April. 相似文献