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101.
采用培育55 d的银新杨、白榆和文冠果营养钵苗在生长季6月、7月和8月不同时间进行造林,测定缓苗期3个树种叶片的光合指标,以期为幼苗缓苗期的管理提供依据。结果表明:3个树种的光合指标均存在显著差异。同一树种不同造林时间的净光合速率无显著差异,蒸腾速率、气孔导度、胞间CO2浓度则存在差异,以7月的值最大。3个树种在不同造林时间的光合特性变化基本一致,净光合速率、蒸腾速率、气孔导度与胞间 CO2浓度在栽后1~3 d出现持续下降,4 d开始回升,5~7 d内恢复到栽前水平。3个树种在不同造林时间的缓苗期为5~7 d,此时应该加强土壤水分的管理,保证水分供应充足,确保幼苗尽快度过缓苗期,提高造林成活率。  相似文献   
102.
鱼类精氨酸营养生理研究进展   总被引:3,自引:0,他引:3       下载免费PDF全文
精氨酸为鱼类的必需氨基酸之一,其在鱼体内参与蛋白质、多胺的合成,促进生长;精氨酸具有促内分泌作用;精氨酸可在体内生成一氧化氮,一氧化氮参与鱼类心血管活动、免疫功能的调节。某些鱼类可能具有自身精氨酸的合成能力。鱼类赖氨酸一精氨酸拮抗存在种问差异。本研究从精氨酸的合成、生理功能、与赖氨酸的关系以及需求量等方面对鱼类精氨酸的营养生理研究进行了综述。[中国水产科学,2006,13(4):679-685]  相似文献   
103.
不同脂肪源对施氏鲟幼鱼血清生化指标的影响   总被引:13,自引:0,他引:13  
在饲料蛋白质水平和能量水平均能满足生长需要的基础上,以不同的油脂(添加水平均为6%)配制成6种试验饲料,投喂施氏鲟幼鱼7周,分析脂肪源对幼鱼血清胆固醇(TC)、甘油三酯(TG)和高密度脂蛋白(HDL-C)的含量和谷丙转氨酶活性(GPT)等生化指标的影响。结果显示:猪油组、葵花籽油组和氧化鱼油组幼鱼的TG和TC都高于鱼油组、混合油组,而其HDL-C、TC含量显著低于鱼油组、混合油组。表明猪油、葵花籽油和氧化鱼油都不利于鲟鱼的脂肪代谢;而鱼油能够调节和平衡鱼体内的脂肪代谢,降低体脂和肝脂的含量,有利于施氏鲟的健康生长。这与不同油脂具有不同的脂肪酸种类和比例有关。  相似文献   
104.
复合诱食剂对牙鲆摄食生长的影响   总被引:11,自引:0,他引:11  
以初始体质量为(3.01±0.04)g的牙鲆(Paralichthys olivaceus)为实验对象,进行为期70 d的摄食生长实验。共配制5种等氮等能饲料,其中以鱼粉和豆粕为蛋白源(豆粕蛋白替代45%的鱼粉蛋白)配制出基础饲料,分别在基础饲料中添加0.0%、0.5%、1.0%和1.5%的复合诱食剂配制成4种豆粕取代饲料,同时以全鱼粉饲料为对照饲料,研究复合诱食剂对牙鲆摄食生长的影响。结果表明,当豆粕取代饲料中诱食剂添加量为1.0%时,牙鲆的摄食率(P<0.05)、饲料效率(P<0.05)、鱼体脂肪含量(P<0.01)以及肝脏、肠胰蛋白酶活力和肠氨肽酶活力(P<0.01)显著高于不添加诱食剂组;当豆粕取代饲料中诱食剂的添加量为0.0%和0.5%时,牙鲆的特定生长率显著低于全鱼粉组(P<0.05);而当添加量为1.0%和1.5%时,牙鲆的特定生长率与全鱼粉组比较没有出现显著差异(P>0.05)。结论认为,当以豆粕蛋白替代45%的鱼粉蛋白时,添加1.0%的复合诱食剂能够显著提高牙鲆幼鱼对饲料的摄食率和生长率。  相似文献   
105.
Virgin female rainbow trout, Oncorhynchus mykiss, were unilaterally ovariectomised at various stages of ovarian development to investigate the effect of the removal of one ovary on subsequent recruitment and growth of follicles in the remaining ovary. The right ovary was removed from groups of 12–15 fish, 12, 7 and 4 months before they were due to ovulate, and the gonadosomatic index and follicle number and size determined just prior to ovulation. There were no differences in fecundity or follicle size in fish unilaterally ovariectomised at 12 and 7 months prior to ovulation compared to the controls. However, in the females unilaterally ovariectomised 4 months prior to ovulation, the remaining ovary either had the normal number of follicles for a single ovary but of a significantly larger size than follicles in the controls, or alternatively had almost 70% more than the normal number of vitellogenic follicles but comprising two distinctly different size populations. Differences in plasma oestradiol-17β concentrations at the final sample were seen only in females unilaterally ovariectomised 4 months prior to ovulation, where the levels were significantly lower than both the sham operated and control fish (p < 0.05). These data show that in the rainbow trout, complete compensatory ovarian hypertrophy following unilateral ovariectomy can occur throughout a major part of ovarian development, but that follicle recruitment is limited to stages up to (and therefore fecundity is determined by) mid-vitellogenesis.  相似文献   
106.
利用同源克隆技术和cDNA末端快速扩增(RACE)技术克隆大黄鱼胞质型磷脂酶A2(cPLA2)基因的cDNA全长.此外,应用实时定量PCR法检测不同日龄大黄鱼仔稚鱼cPLA2基因的表达量.序列分析结果表明:cPLA2基因全长2 550 bp(GenBank登录号:KF006240.1),其中5'端非编码区176 bp,开放阅读框2 169 bp,3'端非编码区205 bp,共编码723个氨基酸.系统进化树分析结果表明:克隆所得大黄鱼cPLA2基因与其他鱼类的cPLA2基因亲缘关系较近,与哺乳动物的亲缘关系较远.定量检测结果表明:大黄鱼仔稚鱼cPLA2基因的表达量随日龄的增加为先显著升高(1、3、7日龄vs.15日龄,P<0.05),在15日龄时达到最高值,随后显著下降(15日龄vs.19日龄,P<0.05),之后趋于平稳.由此可知,大黄鱼从仔稚鱼到幼鱼早期的变形过程中,磷脂分解代谢的关键酶cPLA2基因的表达量呈现有规律的变化,这可能对机体保持体内磷脂的动态平衡、维护细胞膜的流动性具有重要的意义.大黄鱼仔稚鱼cPLA2基因表达量的变化趋势在一定程度上可以衡量大黄鱼消化系统的发育情况.  相似文献   
107.
调查磷矿周围环境和畜禽组织砷含量,为掌握磷矿区周围畜禽产品安全提供依据。采用原子吸收光谱法进行测定。结果表明,环境中砷含量从高到低依次为水体、白菜、土壤、大米、玉米、牧草,而辣椒和萝卜未检出砷;鸡内脏及肌肉砷含量从高到低依次为肾脏、肺脏、心脏、肝脏、脾脏、肌肉;鸭内脏及肌肉砷含量从高到低依次为肾脏、肝脏、肺脏、肌肉、脾脏、心脏;猪内脏及肌肉砷含量从高到低依次为肠、肾脏、肝脏、脾脏,而心脏、肺脏和肌肉未检出。说明磷矿周围饲养的畜禽存在砷污染现象。  相似文献   
108.
氯化钴在大菱鲆饲料中的安全性评价   总被引:1,自引:0,他引:1  
通过研究饲料中的氯化钴对大菱鲆幼鱼的存活、生长性能、血清生理生化指标、抗氧化酶活力、消化酶活力、血清和肝脏中VB12含量、肌肉和肝脏中钴的残留量、肝脏和肠道组织结构的影响来评估氯化钴在海水鱼饲料中的安全性。通过在基础饲料中添加氯化钴制剂来配制4种具有不同氯化钴含量(0、8、40及80 mg/kg,不计结晶水重量)的等氮等能的实验饲料,每个饲料处理设6个重复,每个重复养殖30尾大菱鲆;养殖实验为期10周。实验结果表明:饲料中添加氯化钴对试验鱼的存活没有显著影响(P0.05),但饲料中80 mg/kg的氯化钴显著降低了大菱鲆的特定生长率、终末体重、摄食率、肝体比和脏体比(P0.05);饲料中高剂量氯化钴(40和80 mg/kg)显著降低了大菱鲆血清过氧化氢酶、谷草转氨酶和碱性磷酸酶活力以及大菱鲆胃淀粉酶和胰蛋白酶活力(P0.05);大菱鲆的血糖含量、血清和肝脏中VB12含量、肝脏和肌肉中钴的残留量均随饲料中钴的添加量增加而显著升高(P0.05);饲料中添加氯化钴对大菱鲆的肠道组织结构没有产生明显影响,但饲料中添加40和80 mg/kg的氯化钴增宽了肝脏肝血窦。研究结果表明:实验条件下,氯化钴在大菱鲆饲料中的添加量8 mg/kg是安全的。  相似文献   
109.
This article describes and contrasts the public health response to two human rabies cases: one organ recipient diagnosed within days of symptom onset and the transplant donor who was diagnosed 18 months post‐symptom onset. In response to an organ‐transplant‐related rabies case diagnosed in 2013, organ donor and recipient investigations were conducted by multiple public health agencies. Persons with potential exposure to infectious patient materials were assessed for rabies virus exposure. An exposure investigation was conducted to determine the source of the organ donor's infection. Over 100 persons from more than 20 agencies spent over 2700 h conducting contact investigations in healthcare, military and community settings. The 564 persons assessed include 417 healthcare workers [5.8% recommended for post‐exposure prophylaxis (PEP)], 96 community contacts (15.6% recommended for PEP), 30 autopsy personnel (50% recommended for PEP), and 21 other persons (4.8% recommended for PEP). Donor contacts represented 188 assessed with 20.2% recommended for PEP, compared with 5.6% of 306 recipient contacts recommended for PEP. Human rabies cases result in substantial use of public health and medical resources, especially when diagnosis is delayed. Although rare, clinicians should consider rabies in cases of encephalitis of unexplained aetiology, particularly for cases that may result in organ donation.  相似文献   
110.
Mbuna, the dominant fishes on the rocky shores of Lake Malawi, have become a major ‘model system’ for the study of rapid speciation and adaptive radiation. At least 295 putative species are known, of which more than 200 remain undescribed. There is no good evidence for monophyly in the mbuna, rather mitochondrial DNA phylogenies indicate that they are polyphlyetic with respect to benthic feeding cichlids of the genera Aulonocara, Alticorpus and some species of Lethrinops. Male mbuna hold territories for 18 months or more and breed year‐round. All species are maternal mouthbrooders, but females do not guard free‐swimming young. Mbuna are polygamous (both sexes). There is sexual dimorphism in size, colour and fin length, and many species show within‐population colour polymorphism. Mbuna genera are largely differentiated on the basis of head, jaw and tooth morphology, but congeneric species are generally distinguished by male colour. Many morphologically specialized forms have broad diets and often feed on common easily obtainable resources. While it is likely that dietary and habitat niche partitioning contributes to species coexistence, this has never clearly been demonstrated under experimental conditions. Populations on spatially separated habitat patches are often genetically differentiated, probably because most species are specialized for life on rocky shores, and lack a dispersal phase in their life histories. Males seem to disperse more than females, but are able to home several kilometres back to their territories. Some closely related ecologically equivalent allopatric populations are differentiated in male colour. Those tested have been shown to mate at least partially assortatively. Sexual selection acting on male colour seems the most plausible mechanism for initial species divergence. The same colour forms seem to have arisen several times, suggesting frequent parallel evolution. The main conservation threat to mbuna at present seems to be translocation of species within the lake as a result of the aquarium trade. However, deleterious effects on indigenous populations are not documented. In the long run, sedimentation, pollution, introduction of alien species and the development of targeted food fisheries could be more serious threats.  相似文献   
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