小麦叶锈菌生理小种MFR的分子鉴定研究   总被引：2，自引：0，他引：2  

蒲志刚  刘太国  张敏  陈万权 《植物病理学报》2004,34(5):449-454

 用AFLP方法对来自中国和墨西哥的23个小麦叶锈菌生理小种进行分析,共筛选了64对引物,获得一对引物(M₀₅/E₀₃)可在MFR小种中扩增出一条特异性DNA片段,进行回收、克隆、测序,结果表明该片段具有325个碱基。根据特异性片段序列设计出SCAR标记引物,对60个叶锈菌生理小种分离物进行回检结果表明,研制的SCAR标记能够准确区分MFR生理小种。本实验结果为小麦锈菌生理小种分子检测体系的建立奠定了基础  相似文献   

菜豆新品种将军油豆的选育   总被引：1，自引：1，他引：1  

冯国军  曲敏  刘大军  闫田柱  王玉莉  叶永亮 《中国蔬菜》2004,1(5):22-23

将军油豆是以农家品种大马掌为母本 ,家雀蛋为父本人工杂交后系统选育而成。中熟 ,蔓生 ,从播种到采收 6 5d(天 ) ,生长势强 ,嫩荚绿色 ,扁条形 ,荚尖部有红条纹 ,平均荚长 2 0cm ,荚宽 2 .4cm ,单荚质量 2 4g ,纤维少 ,肉质面 ,属典型的东北优质油豆角类型。抗炭疽病、锈病 ,适应性广 ,春秋皆可种植 ,露地、保护地兼用  相似文献   

体验经济与内蒙古草原旅游开发   总被引：10，自引：0，他引：10  

刘敏  石学勇 《干旱区资源与环境》2004,18(5):128-131

本文通过捕捉近年来消费情况的变化 ,认为应对体验经济引起重视。在介绍体验经济的提出 ,体验的概念和体验的组成部分的基础上 ,提出体验经济对旅游具有创造差异化竞争优势、准确把握市场、使旅游者更舒适愉悦等意义 ,并在体验经济理论下结合内蒙古草原旅游的实际情况 ,提出内蒙古草原的体验设计和营销策略  相似文献   

Ginkgo biloba extract enhances c-jun expression and attenuates motoneuron death induced by root avulsion     

LI Fang-lan  ZHOU Li-hua  YUAN Qun-fang  YAO Zhi-bin  WU Wu-tian 《园艺学报》2004,20(10):1783-1785

AIM: To investigate the influence of Ginkgo biloba extract (EGb761) on c-jun expressions and motoneurons survival following root avulsion. METHODS: One hundred and eighty adult Sprague-Dawley female rats were randomly divided into control and EGb761 groups. Immediately after avulsion of C5-T1 nerve roots, the rats were injected ip with either 1 mL of EGb761 25 mg·kg^-1·d^-1 or the same volume of normal saline, and the treatment repeated everyday. At 4 h to 6 weeks following avulsion, the C7 spinal segments of all rats were collected and prepared for c-jun immunocytochemistry and neutral red stain. The numbers of c-jun positive and survival motoneurons were counted and compared between two groups at each time point. RESULTS: In control rats following avulsion, c-jun positive motoneurons appeared at 4 h, reached its maximum at 1 d and declined to 2 weeks. Avulsion-induced motoneurons death started at 2 weeks, climbed to its maximum at 4 weeks-6 weeks. In EGb761 treated rats, both numbers of c-jun positive and survival motoneurons were more than that in control group at each time point. CONCLUSION: EGb761 attenuates avulsion-induced motoneurons death, and this effect may be related to up-regulation of c-jun gene in avulsed motoneurons.  相似文献   

胡杨内生真菌曲霉(Aspergillus sp.)链格孢(Alternaria sp.)的生理特性     

袁秀英  韩艳洁  姜海燕  白红霞 《干旱区资源与环境》2004,18(6):170-173

采用接种法 ,对胡杨内生真菌曲霉 ( Aspergillus sp.)和链格孢 ( Alternaria sp.)在不同温度、p H、碳源、氮源、培养基及糖质量浓度下进行了生理实验研究。结果表明 :温度对两种菌株的生长和孢子产生具有明显的影响 ;曲霉 ( Aspergillus sp.) p H5～ 6时生长良好 ,而链格孢 ( Alternaria sp.) p H为 4～ 5时菌株生长良好 ,两种菌株均以蔗糖为主要碳源 ;菌丝干质量与培养基质量浓度呈正向关系。  相似文献   

兰州市SO_2污染控制区优化规划方案的探讨     

王颖  袁九毅  何治 《干旱区资源与环境》2004,(Z2)

根据《大气法》第二十七条关于划定酸雨控制区和SO2 污染控制区的规定 ,为使兰州市SO2 控制区达到环境质量目标的要求 ,本文以 98年的污染源资料和气象资料为模拟对象 ,利用大气扩散模式 ,通过各个污染源对每个控制点浓度分担率的计算 ,以污染源的总削减两最小 (总排放量最大 )或总削减率最小为优化目标 ,列出了几种优化方案并利用线性规划的方法对各种结果进行了对比分析。为了使最终的优化结果切实可行 ,将点源的上界乘一经济技术指标值 ,既可以使控制区达到环境质量目标值 ,又充分体现了公平合理的原则。  相似文献   

二棘血蜱叮咬中华硬蜱纯化抗原免疫接种宿主后的交叉免疫反应     

刘志刚  叶炳辉  崔晓民  徐敏 《中国生物防治》2004,20(2):95-98

选用中华硬蜱105kD柱层析纯化抗原对新西兰兔进行免疫接种,再用二棘血蜱进行叮咬,并与单纯二棘血蜱叮咬组和佐剂对照组进行对比,以探讨不同种属硬蜱之间的交叉免疫抗性。结果显示:单纯二棘血蜱叮咬组吸血量为181 3±44 3mg,而二棘血蜱叮咬由中华硬蜱105kD纯化抗原免疫接种组和佐剂对照组新西兰兔后其吸血量分别为102 1±25 3mg和168 5±40 9mg,纯化抗原免疫接种组较单纯二棘血蜱叮咬组和佐剂对照组吸血量分别下降43 7%和39 4%(P<0 01)。同时,纯化抗原免疫接种组也较单纯二棘血蜱叮咬组和佐剂对照组产卵量有显著下降。该研究结果表明中华硬蜱和二棘血蜱之间存在着交叉免疫反应。  相似文献   

Differential regulating effect on estrogen receptor α and β expression in female rat hippocampus and cortex regions between different types of estrogen regents     

JIN Min  HUANG He-feng  JIN Fan 《园艺学报》2004,20(8):1422-1426

AIM: To study the regulatory effect two different estrogen reagents on expressions of estrogen receptor α and β in female rat hippocampus and cortex regions. METHODS: 12 cycles after ovariectomy, female rats were orally injected with premarin or progynova for 3 cycles before sacrificed. Semiquantitative RT-PCR was used to detect the ERs mRNA expression and SP immunohistochemistry was performed to measure the ERs protein distribution and expression. RESULTS: In premarin group, ER α mRNA levels in both hippocampus and cortex tissues decreased significantly compared with control. ER α protein level in hippocampus was lower than that in the control. However, ER α protein level in cortex had no statistical difference. ER β mRNA in the two regions and ER β protein in cortex had no statistical differences compared with control, while ER β protein level in hippocampus was higher than that in the control. In progynova group, both mRNA and protein levels of ER β increased significantly in the two regions compared with the control, and ER α mRNA level also increased in hippocampus, but ER α mRNA level in cortex and ER α protein levels in the above two regions showed no statistical differences. CONCLUSION: There were differential regulatory effects on ER α and ER β expression in female rat cognitive regions between the two different types of estrogen reagents, which may be one of the mechanisms of varied effects in different estrogen replacement therapy reagents.  相似文献   

Advances in studying the differentiation into neurons of mesenchymal stem cells and their application     

YUAN Qing-tao  DENG Yu-bin  LI Shu-nong 《园艺学报》2004,20(11):2139-2142

Mesenchymal stem cells (MSCs) are a population of multipotent cells that can proliferate and differentiate into marrow and non-marrow cell types, such as adipocytes, chondrocytes, myocytes, and so on. In recent years, many researchers have studied whether MSCs are capable of differentiation into neurons in vivo and ex vivo. The result that MSCs-derived neurons express NSE and NF, but don't express GFAP suggests MSCs can differentiate into neurons, some researchers have achieved success in promoting functional recovery in Pakinsons and transactional spinal cord injury rat models by use of MSCs-derived neurons. Therefore, MSCs-derived neurons will play an important role in the therapy for a variety of diseases of the nervous system.  相似文献   

鸭瘟病毒强毒株在急性人工感染成年鸭病例体内分布规律   总被引：7，自引：3，他引：7  

刘菲  程安春  汪铭书  宋涌  廖永洪  袁桂萍  韩晓英  徐超 《中国兽医学报》2004,24(3):228-230

5 6只 3月龄四川麻鸭经皮下接种鸭瘟病毒 (DPV)强毒 SC1株 ,成功建立了 DPV感染的急性病理模型 ,并应用PCR方法检测了不同时间 DPV在感染鸭体各组织器官的分布情况。结果表明 ,接种 2 h后 ,即能够从脑、肝、脾、法氏囊、胸腺中检出 DPV DNA;12 h,可从心脏、肝脏、脾脏、肺脏、肾脏、十二指肠、直肠、法氏囊、胸腺、胰腺、脑、胸肌、食管、腺胃、血液、舌、口腔分泌物、皮肤、骨髓和粪便等检测到 DPV的 DNA。检出时间最早和检出率最高的组织器官为肝脏和脑组织。本试验为阐明 DPV的致病机理和应用 PCR方法检测感染鸭体组织中的 DPV提供了重要的实验数据。  相似文献