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Enterohemorrhagic Escherichia coli (EHEC) are a subgroup of Shiga toxin-producing Escherichia coli (STEC) that are able to cause serious food-borne intestinal diseases which can be followed in 5 to 15% by extraintestinal sequelae such as the hemolytic-uremic syndrome (HUS). One of the major pathogenicity factors of EHEC is the production of one or more Shiga toxins (Stx), which act as inhibitors of protein biosynthesis and have profound effects on the signal transduction and immunological response in eukaryotic cells. The stx genes are located in the genome of heterogeneous, lambdoid, functional or cryptic bacteriophages and are expressed during the phage life cycle. Due to the linkage between the phage life cycle and stx expression, STEC and their bacteriophages are useful as a model for the analysis of stress response and virulence of this food-borne pathogen.  相似文献   
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Landscape Ecology - Harmful effects of habitat loss and fragmentation can be detected across multiple spatial scales, yet most studies that aim to characterize these effects take place at a single...  相似文献   
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The perennial cup plant (Silphium perfoliatum L.) is considered as an alternative feedstock to maize for biogas plants. Due to its ecological advantages of an extensive management and function as food resource for pollinators, it can be grown in Ecological Focus Areas (EFAs) since 2018. However, studies at the Julius Kühn-Institute in Braunschweig (Germany) showed that the assumed advantage of the cup plant of a high drought tolerance could not be confirmed regarding aspects of crop production and yield. We complemented this experiment by assessing how different soil moisture conditions affect the production of floral resources and insect visitation. In 2014, we sampled three irrigated and three rainfed plots of the cup plant. We quantified the nectar volume and sugar mass per inflorescence, the number of inflorescences per plant and calculated the total nectar sugar production. We further counted insect visitation on the inflorescences. Due to reduced numbers of inflorescences per plant and an earlier harvest, the estimated nectar sugar production was 58 kg/ha regarding irrigated and 20 kg/ha regarding rainfed plots. Honeybee visitation per inflorescence was about twice as high in the irrigated plots. Furthermore, the early harvest is a disadvantage for wild pollinators with a late activity period.  相似文献   
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Seed crops of white clover (Trifolium repens L.) are usually established with a cover crop. Provided sufficient light, white clover may compensate for low plant density by stoloniferous growth. Our objectives were (1) to compare spring barley or spring wheat used as cover crops for white clover and (2) to find the optimal seeding rate/row distance for white clover. Seven field trials were conducted in Southeast Norway from 2000 to 2003. Barley was seeded at 360 and 240 seeds m?2 and wheat at 525 and 350 seeds m?2. White clover was seeded perpendicularly to the cover crop at 400 seeds m?2/13 cm row distance or 200 seeds m?2/26 cm. Results showed that light penetration in spring and early summer was better in wheat than in barley. On average for seven trials, this resulted in 11% higher seed yield after establishment in wheat than in barley. The 33% reduction in cover crop seeding rate had no effect on white clover seed yield for any of the cover crops. Reducing the seeding rate/doubling the row distance of white clover had no effect on seed yield but resulted in slightly earlier maturation of the seed crop.  相似文献   
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The response of soil microbial communities following changes in land-use is governed by multiple factors. The objectives of this study were to investigate (i) whether soil microbial communities track the changes in aboveground vegetation during succession; and (ii) whether microbial communities return to their native state over time. Two successional gradients with different vegetation were studied at the W. K. Kellogg Biological Station, Michigan. The first gradient comprised a conventionally tilled cropland (CT), mid-succession forest (SF) abandoned from cultivation prior to 1951, and native deciduous forest (DF). The second gradient comprised the CT cropland, early-succession grassland (ES) restored in 1989, and long-term mowed grassland (MG). With succession, the total microbial PLFAs and soil microbial biomass C consistently increased in both gradients. While bacterial rRNA gene diversity remained unchanged, the abundance and composition of many bacterial phyla changed significantly. Moreover, microbial communities in the relatively pristine DF and MG soils were very similar despite major differences in soil properties and vegetation. After >50 years of succession, and despite different vegetation, microbial communities in SF were more similar to those in mature DF than in CT. In contrast, even after 17 years of succession, microbial communities in ES were more similar to CT than endpoint MG despite very different vegetation between CT and ES. This result suggested a lasting impact of cultivation history on the soil microbial community. With conversion of deciduous to conifer forest (CF), there was a significant change in multiple soil properties that correlated with changes in microbial biomass, rRNA gene diversity and community composition. In conclusion, history of land-use was a stronger determinant of the composition of microbial communities than vegetation and soil properties. Further, microbial communities in disturbed soils apparently return to their native state with time.  相似文献   
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The soil organic carbon (SOC) pool of the Northern Hemisphere contains about half of the global SOC stored in soils. As the Arctic is exceptionally sensitive to global warming, temperature rise and prolonged summer lead to deeper thawing of permafrost‐affected soils and might contribute to increasing greenhouse gas emissions progressively. To assess the overall feedback of soil organic carbon stocks (SOCS) to global warming in permafrost‐affected regions the spatial variation in SOCS at different environmental scales is of great interest. However, sparse and unequally distributed soil data sets at various scales in such regions result in highly uncertain estimations of SOCS of the Northern Hemisphere and here particularly in Greenland. The objectives of this study are to compare and evaluate three controlling factors for SOCS distribution (vegetation, landscape, aspect) at two different scales (local, regional). The regional scale reflects the different environmental conditions between the two study areas at the coast and the ice margin. On the local scale, characteristics of each controlling factor in form of defined units (vegetation units, landscape units, aspect units) are used to describe the variation in the SOCS over short distances within each study area, where the variation in SOCS is high. On a regional scale, we investigate the variation in SOCS by comparing the same units between the study areas. The results show for both study areas that SOCS are with 8 kg m?2 in the uppermost 25 cm and 16 kg m?2 in the first 100 cm of the soil, i.e., 3 to 6 kg m?2 (37.5%) higher than existing large scale estimations of SOCS in West Greenland. Our approach allows to rank the scale‐dependent importance of the controlling factors within and between the study areas. However, vegetation and aspect better explain variations in SOCS than landscape units. Therefore, we recommend vegetation and aspect for determining the variation in SOCS in West Greenland on both scales.  相似文献   
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