5-Lipoxygenase expression in benign and malignant canine prostate tissues     

Goodman LA  Jarrett CL  Krunkosky TM  Budsberg SC  Northrup NC  Saba CF  LeRoy BE 《Veterinary and comparative oncology》2011,9(2):149-157

5-Lipoxygenase (5-LO) is overexpressed in human prostate carcinomas (PCs), and its inhibition decreases proliferation and induces apoptosis in prostate cancer cell lines. We hypothesized that 5-LO would be overexpressed in canine PC compared with benign prostate tissue and may be important in the pathogenesis of the disease. Immunoblot analysis of canine PC and benign prostatic hyperplasia (BPH) tissues demonstrated 5-LO expression in both. 5-LO immunohistochemical staining was not significantly different within the stromal or epithelial components of canine primary PC, BPH or suppurative prostatitis, suggesting that differential expression of this enzyme does not occur in these conditions. The percentage of tumour cells expressing 5-LO was significantly lower in metastatic PC lesions compared with primary PC (P < 0.0001). This decreased expression may indicate down-regulation or altered expression of the enzyme with progression of canine PC to a metastatic phenotype.  相似文献   

Single-agent gemcitabine chemotherapy in dogs with hepatocellular carcinomas     

Elpiner AK  Brodsky EM  Hazzah TN  Post GS 《Veterinary and comparative oncology》2011,9(4):260-268

The goal of this study was to determine the efficacy and tolerability of gemcitabine in dogs diagnosed with hepatocellular carcinoma (HCC). Eighteen dogs were examined retrospectively (4 massive HCC, 10 nodular HCC and 4 diffuse HCC). All dogs received gemcitabine at 350-400 mg m(-2) weekly for 5 weeks. Toxicity was graded using VCOG-CTCAE guidelines and response was monitored with serial abdominal ultrasounds. Fifteen dogs completed all five cycles. Toxicity was minimal and consisted of grade I/II vomiting, anorexia and diarrhoea and two episodes of grade III neutropenia. Median survival time for all dogs was 983 days. Median progression free interval was 971 days. Based on the results of this study, surgery remains the best treatment for HCC, despite incomplete resection. There was no improvement in the survival of those diagnosed with nonresectable HCC treated with gemcitabine chemotherapy.  相似文献   

Radiosensitivity and capacity for radiation-induced sublethal damage repair of canine transitional cell carcinoma (TCC) cell lines     

Parfitt SL  Milner RJ  Salute ME  Hintenlang DE  Farese JP  Bacon NJ  Bova FJ  Rajon DA  Lurie DM 《Veterinary and comparative oncology》2011,9(3):232-240

Understanding the inherent radiosensitivity and repair capacity of canine transitional cell carcinoma (TCC) can aid in optimizing radiation protocols to treat this disease. The objective of this study was to evaluate the parameters surviving fraction at 2 Gy (SF(2) ), α/β ratio and capacity for sublethal damage repair (SLDR) in response to radiation. Dose-response and split-dose studies were performed using the clonogenic assay. The mean SF(2) for three established TCC cell lines was high at 0.61. All the three cell lines exhibited a low to moderate α/β ratio, with the mean being 3.27. Two cell lines exhibited statistically increased survival at 4 and 24 h in the dose-response assay. Overall, our results indicate that the cell lines are moderately radioresistant, have a high repair capacity and behave similarly to a late-responding normal tissue. These findings indicate that the radiation protocols utilizing higher doses with less fractionation may be more effective for treating TCC.  相似文献   

鼻咽癌来源LMP1转基因小鼠的制备     

蓝轲  乔贵林  沈新民  张玲  吕丽春  姚开泰 《动物医学进展》2002,23(1):69-71

本试验利用显微注射技术，制备鼻咽癌来源LMP1基因（N-LMP1）的转基因小鼠。共获得15只首建鼠，经小鼠尾部组织DNA分析，PCR法证明其中两只小鼠有N-LMP1基因扩增带，Southern杂交显示PCR阳性小鼠有特异的杂交信号，整合率为13.3%，N-LMP1转基因小鼠的建立为研究N-LMP1转基因小鼠的建立为研究N-LMP1在鼻咽癌变过程中的作用机制奠定了基础。  相似文献   

Multicentric Squamous Cell Carcinomas In Situ Resembling Bowen's Disease in Five Cats     

WILLIAM H. MILLER Jr.  VERENA AFFOLTER†  DANNY W. SCOTT  MAJA M. SUTER† 《Veterinary dermatology》1992,3(4-5):177-182

Abstract— Multiple squamous cell carcinomas in situ were diagnosed in five aged cats. Despite clinical courses ranging from 18 to 30 months, there was no clinical or histologic evidence of breachment of the basement membrane. The condition closely resembles Bowen's disease in humans.
Résumé— Des épithéliomas spinocellulaires multiples in situ ont été diagnostiqués chez 5 chats. Maigré une anclenneté clinique de 18 à 30 mois, il n'y avait pas de signes cliniques ou histologiques d'atteinte de la membrane basale. Cette affection resemble á la maladie de Bowen chez l'homme.
Zusammenfassung— Bei fünf alten Katzen wurden multiple plattenepithelkarzinome in situ diagnostiziert. Trotz der klinischen Krankheitverläufe von 18 bis 30 Monaten konnte weder klinisch noch histologisch ein Durchbrechen der Basalmembran nachgewiesen werden. Dieses Bild ähnelt sehr dem Bowens Krankheit des Menschen.
Resumen  En cinco gatos se diagnosticaron carcinomas multiples de células escamosas in situ. A pesar del curso clínico de 18–30 meses no se observó evidencia clinica, o histológica de infiltración a traves de la membrana basal. La condición es muy semejante a la enfermedad de Bowen en medicina humana.  相似文献   

人类表皮生长因子受体2蛋白在宫颈癌组织中的表达及意义     

胡玲  秦江霞  王天红  陈敦金 《湛江医学院学报》2012,30(4):380-382

目的观察宫颈癌组织中人类表皮生长因子受体2（HER-2/neu）蛋白表达及其在宫颈癌发生发展中的作用。方法应用免疫组织化学方法检测20例正常宫颈组织、30例宫颈上皮内瘤样病变（CINⅠ-Ⅲ）和50例宫颈癌组织中HER-2/neu蛋白表达。结果宫颈癌组织中HER-2/neu蛋白表达显著高于宫颈上皮内瘤样病变（P〈0.01）,正常宫颈组织未见阳性表达;宫颈癌组织中HER-2/neu蛋白表达与肿瘤浸润深度、淋巴结转移、临床分期密切相关（P〈0.05）。结论 HER-2/neu蛋白表达上调可能在宫颈癌发生发展中起重要作用。  相似文献   

肝癌细胞靶向肽的筛选与鉴定     

赵红蕾  韩文瑜  刁昱文  冯新  韩东  朱僧  刘晓贺  路荣  雷连成 《兽医大学学报》2012,(2):202-206

利用噬菌体环七肽库筛选与肝癌细胞特异性结合的多肽,并对其亲和力进行生物学鉴定。以HL-7702为消减细胞,HepG2为筛选靶细胞,对噬菌体随机环七肽库进行4轮全细胞消减筛选,并随机挑取60个阳性噬菌体克隆,以ELISA法鉴定其与HepG2细胞的结合活性,并取阳性克隆进行测序分析,并合成多肽进行免疫细胞化学染色鉴定。经4轮筛选,噬菌体在靶细胞HepG2上出现明显富集;利用ELISA从随机挑选的60个噬菌体克隆中得到15个与肝癌细胞具有高结合力的阳性克隆,测序并进行序列分析比对发现氨基酸序列无同源性,经免疫细胞化学染色鉴定后,发现1条多肽序列亲和力较高,为提高抗菌肽对肿瘤细胞的靶向杀伤作用奠定基础。  相似文献   

犬基底细胞癌的病理组织学观察     

潘俊斌  刘博奇  林凯  于博 《畜牧兽医杂志》2012,(3):20-22

基底细胞癌(basal cell carcinoma,BCC)是一种常见的低度皮肤恶性肿瘤,又名基底细胞上皮癌、基底细胞癌和侵蚀性溃疡。本文主要探讨皮肤基底细胞癌的临床病理诊断和鉴别诊断要点,为提高基底细胞癌的诊治水平提供依据。通过病理组织学观察,皮肤破溃、出血,肿瘤细胞成团块状增生,由结缔组织分割为不规则小叶状。细胞呈梭形、多边形及近圆形;细胞核圆形,核膜清晰,有一明显核仁,有一定异形性,偶见分裂相,作为确诊基底细胞肉瘤的主要依据,并对疾病的处理和治疗方法进行了归纳和总结。  相似文献   

p27基因在宫颈癌组织中的表达及其意义     

王祥珍  李英勇黄颢 《湛江医学院学报》2005,23(4):364-365,367

目的：探讨肿瘤抑制基因p27在宫颈癌组织中的表达及其临床意义。方法：应用免疫组织化学方法（SP法）检测p27基因在62例官颈癌组织和20例正常官颈组织中的表达。结果：62例宫颈癌组织中,p27的阳性表达率为38．7％（24／62）;p27的阳性表达与宫颈病灶大小、临床分期、细胞分化程度、转移及预后有密切关系（P〈0．01或〈0．05）;与宫颈癌的病理类型及年龄无关（P〉0．05）。结论：p27基因的表达与宫颈癌的生长、浸润及淋巴结转移有关,其表达水平的高低可作为判断宫颈癌预后的重要指标之一。  相似文献   

双歧杆菌完整肽聚糖的体外抗肿瘤作用及对大鼠大肠肿瘤的影响   总被引：1，自引：0，他引：1  

蔡良真李姝君  官成浓 《湛江医学院学报》2005,23(5):500-502

目的：观察双歧杆菌完整肽聚糖（WPG）体外抗肿瘤作用及对大鼠大肠肿瘤的影响。方法：采用MTT法检测双歧杆菌WPG对人结肠癌细胞株LS-174T、人胃粘膜上皮细胞株GES-1的杀伤作用。以二甲肼（DMH）为诱发荆,观察双歧杆菌WPG对大鼠大肠肿瘤的防治作用;DMH诱癌及双歧杆菌WPG灌胃时间为18周,在第33周结束实验。结果：双歧杆菌WPG在体外对人结肠癌细胞株具有显著的选择性杀伤作用,体内给药可明显降低大鼠大肠肿瘤发生率（60％vs100％,P〈0．01）．但大肠癌平均个数及肿瘤体积指数的差异无显著性（P〉0．05）。结论：双歧杆菌的WPG对DMH诱导的大鼠大肠肿瘤具有预防作用。  相似文献