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裂蹄木层孔菌发酵产物抑菌活性的研究 总被引:1,自引:1,他引:0
为了研究裂蹄木层孔菌发酵产物对植物病原菌的抑菌活性。采用菌丝生长速率法测定裂蹄木层孔菌不同发酵时间的产物对7种供试病原菌的抑制作用,将裂蹄木层孔菌的发酵产物进行不同的处理,分级纯化抑菌活性多糖,比较其抑菌活性。结果表明:裂蹄木层孔菌发酵产物对7种病原菌均有抑制作用;发酵时间为20天的裂蹄木层孔菌发酵产物对杨树烂皮病的抑制率最高;裂蹄木层孔菌发酵产物的主要抑菌活性物质存在于发酵上清液醇提物中;醇沉级别为30%(浓度为50 mg/mL)醇提物中的多糖对病原菌的抑制率为供试醇沉级别中最高。说明裂蹄木层孔菌含有抑制病原菌的有效成分,具备成为新型生物农药的潜力。 相似文献
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野生桑黄菌固体培养基配方的优化试验 总被引:1,自引:0,他引:1
桑黄菌是一种具有重要开发价值的药用真菌。以菌丝日均生长速度为指标,比较野生桑黄菌在玉米粉综合培养基、马铃薯综合培养基、木生菌标准培养基上的生长情况,结果表明野生桑黄菌在玉米粉综合培养基上的日均生长速度最快。在选择葡萄糖为碳源,玉米粉+蛋白胨为氮源的基础上,通过L9(34)正交试验设计优化野生桑黄菌固体培养基的配方为:20g/L葡萄糖,20g/L玉米粉,1g/L蛋白胨,0.3g/L MgSO4,2g/L KH2PO4,20g/L琼脂。 相似文献
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Phellinus tremulae is an important fungal decay agent common to aspen and a critical component to the cavity-nesting bird complex found in western aspen stands. Little information exists on the conditions that facilitate infection and spread of P. tremulae in aspen forests. I used Forest Inventory and Analysis (FIA) data to explore the relationships of several tree and stand characteristics to the presence and frequency of P. tremulae in aspen measured across several western states of the United States. Results suggest a strong relationship between tree age, tree diameter, and compacted crown ratio with infection frequency in trees while stand purity, canopy cover and stand age had a positive relationship with the occurrence of P. tremulae in forest stands containing aspen. Logistic regression modeling identified stand age as the only variable that increased the odds of predicting infection at the stand-level while all tree-level variables were included in the tree model. Data also show that infection rates in the study area were lower than in other parts of aspen's range, and that average size of infected trees was smaller in the study area than those reported elsewhere. These results have important implications to management of aspen for wildlife, especially for birds that use decayed aspen for nesting. 相似文献
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Composition and proposed structure of a water-soluble glycan from the Keumsa Sangwhang Mushroom (Phellinus linteus) 总被引:1,自引:0,他引:1
Phellinus linteus is a mushroom which has been prized in Korea for its medicinal properties since ancient times. A glycan preparation from this source has shown promise in experimental anti-tumour and metastasis studies, but the glycan has not been well characterized. In the present work, the glycan fraction of a hot water extract from P. linteus (Keumsa Sangwhang Mushroom, Korea) has been isolated following ammonium sulfate precipitation, dialysis/concentration and anion exchange chromatographic steps. Analyses for monosaccharide composition showed glucose and mannose to be the major constituents. Digestion of the glycan fraction with specific glycosidases and by linkage analysis allowed us to propose a core beta(1-3) linked glucan heavily substituted via (1-6) links with beta(1-3) linked mannose chains. Significant levels of galactose and xylose, present in the glycan fraction, may be associated with this glucomannan or not. These findings are consistent with the view that a core beta(1-3)-linked glucan chain with beta(1-6) branch points is a common feature of mushroom glycans possessing anti-tumour activity. 相似文献
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桑黄子实体多糖提取工艺及单糖组成研究 总被引:1,自引:0,他引:1
通过单因素试验和正交试验,从提取温度、料液比、提取次数、提取时间4个方面研究桑黄子实体多糖提取条件,得出最佳条件为:提取温度95℃,料液比1∶50,提取次数2次,提取时间2h。运用薄层色谱和气相色谱分析单糖组成,日本桑黄子实体多糖的单糖组成为鼠李糖、阿拉伯糖、葡萄糖、甘露糖和半乳糖;韩国桑黄子实体多糖的单糖组成为鼠李糖、阿拉伯糖、木糖、葡萄糖、甘露糖和半乳糖。 相似文献
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研究采用水提醇沉法得到桑黄菌丝多糖(大分子量粗多糖),通过小鼠抗疲劳、耐缺氧能力实验,研究桑黄菌丝多糖的生理活性。以小鼠游泳力竭时间、游泳前后血乳酸、肝糖原以及血清尿素氮含量的变化为指标,考察桑黄多糖抗疲劳能力,以小鼠在缺氧和亚硝酸钠中毒环境下的存活时间为指标,考察桑黄多糖耐缺氧能力。结果表明,灌胃桑黄多糖可以显著延长小鼠游泳时间达70.62%,降低游泳后乳酸曲线下面积22.89%,提高肝糖原储备33.36%,减少游泳后血清尿素氮含量23.17%,延长小鼠在缺氧环境下42.08%和亚硝酸钠中毒环境下35.29%的存活时间。因此,桑黄多糖具有提高小鼠抗疲劳和耐缺氧能力的作用,且在一定剂量范围内,实验效果与灌胃剂量呈正效应关系。 相似文献
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