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61.
Secretoglobin and Transferrin Expression in Bronchoalveolar Lavage Fluid of Horses with Chronic Respiratory Disease 下载免费PDF全文
M. Miskovic Feutz L.L. Couetil C.P. Riley X. Zhang J. Adamec R.E. Raskin 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(6):1692-1699
Background
Lower expression of secretoglobin and transferrin has been found in the bronchoalveolar lavage fluid (BALF) of a small number of horses with experimentally induced signs of recurrent airway obstruction (RAO) compared to healthy controls.Hypothesis/Objectives
Secretoglobin and transferrin BALF expression will be similarly decreased in horses with naturally occurring clinical signs of RAO and in horses with experimentally induced clinical signs of RAO as compared to healthy controls and intermediate in horses with inflammatory airway disease (IAD).Animals
Recurrent airway obstruction‐affected and control horses were subjected to an experimental hay exposure trial to induce signs of RAO. Client‐owned horses with a presumptive diagnosis of RAO and controls from the same stable environments were recruited.Methods
Pulmonary function and BALF were evaluated from control and RAO‐affected research horses during an experimental hay exposure trial (n = 5 in each group) and from client‐owned horses (RAO‐affected horses, n = 17; IAD‐affected horses, n = 19; healthy controls, n = 5). The concentrations of secretoglobin and transferrin in BALF were assessed using Western blots.Results
Naturally occurring and experimentally induced RAO horses had similar decreases in BALF transferrin expression, but secretoglobin expression was most decreased in naturally occurring RAO. Secretoglobin and transferrin expression were both lower in BALF of RAO‐affected horses than in IAD‐affected and control horses.Conclusions and Clinical Importance
Secretoglobin and transferrin expression is decreased in BALF of RAO‐affected horses after both experimental and natural exposure. Secretoglobin and transferrin likely play clinically relevant roles in the pathophysiology of RAO, and may thus be used as biomarkers of the disease. 相似文献62.
西印度洋是海洋捕捞作业的重要海域,分析捕捞方渔获种类组成和渔获量是评价渔业资源利用状态的基础,也是确保其资源可持续开发和科学管理的重要支撑。本研究根据FAO提供的1950-2019年西印度洋海域渔获量数据,采用优势分析(Dominance analysis),分析了70年间主要捕捞方渔获种类和渔获量组成,比较不同时间序列下主要捕捞方和种类对渔获量的贡献率。研究表明,西印度洋海域渔获量整体呈上升趋势,根据突变点检测及渔获量增长趋势可划分为1950-1982年、1983-2005年和2006-2019年三个阶段。研究分析表明,各阶段累计渔获量前十的渔获种类、捕捞方不存在显著性差异(P<0.05)长头小沙丁鱼(Sardinella longiceps)累计渔获量为最高,鲣、黄鳍金枪鱼等种类渔获量对总渔获量的贡献度较高;印度、巴基斯坦和伊朗等是沿海国和地区各阶段渔获量最高的捕捞方,西班牙、法国、日本和中国台湾省等是非沿海国和地区渔获量最高的捕捞方,对各阶段印度洋西部海域总渔获量的贡献度基本都超过10%。。研究认为,基于印度洋西部海域渔业资源开发现状及其潜力,各主要捕捞国家和地区应该降低捕捞努力量,养护已过度开发的经济种类,增加对资源量丰富的短生命周期种类的开发力度,确保印度洋西部海域渔业资源可持续利用。 相似文献
63.
因受特定的历史背景、人文地理和气候环境的影响,近代广东住宅园林独具特色,既继承了传统岭南园林的环境适应性和人文要素,又扬弃融合了西方园林的造园手法。文章着重研究近代广东住宅园林的布局特点,特别是中西合璧的造园手法所形成的布局样式和风格,为现代园林的设计与实践提供新的视角。 相似文献
64.
The insulin-like growth factor I (IGF-I) cDNA (GenBank Accession No. AY247412) of triangular bream (Megalobrama terminalis) was expressed for the first time in Escherichia coli. To construct the expression plasmid, the IGF-I cDNA was subcloned into prokaryotic-expressing vector pGEX-4T-1. The E.coli JM109 was transformed with the recombinant plasmid pGEX-4T-1-IGF-I, and the transgene expression was observed after being induced with isopropyl-β-D-thiogalactoside (IPTG). The results of SDS polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting indicated that the recombinant fusion protein had immune activity and the molecular weight was about 47 kDa. The results of SDS-PAGE and thin layer scanning showed that the yield of fusion protein had been enlarged with prolonging time. When the time of induced expression was 1, 2, 3, 4, 5, and 6 h, the expression amount was approximately 1.4, 4.3, 8.1, 11.3, 16.3 and 18.8% of total bacterial protein respectively. 相似文献
65.
Excavating sponges that are destructive to farmed pearl oysters in Western and Northern Australia 总被引:1,自引:0,他引:1
Species of the family Clionaidae (Porifera: Demospongiae) that excavate shell of the silver‐lip pearl oyster, Pinctada maxima, in north Western and Northern Australia are described. Two species belong to the genus Cliona and one species to the closely related genus Pione. Cliona orientalis has only recently been reported from Australia in living and dead coral on the Great Barrier Reef, and this is the first report of this species from north Western and Northern Australia. Cliona dissimilis is reported from Australia for the first time. Pione velans was first described from Shark Bay, Western Australia and this study extends its distribution from Albany, south Western Australia to Port Bremer in the Northern Territory. The most common species found was C. dissimilis. Pione velans and C. orientalis were also present, although the latter species was rare. Cliona dissimilis and P. velans were found to be sexually reproductive in some shells with specimens of C. dissimilis with oocytes in May and P. velans in September 1999. Egg development was synchronous, indicating that the sponges were oviparous and would broadcast gametes in a spawning event. 相似文献
66.
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68.
应用Gateway重组技术构建CSBV非结构蛋白,RNA依赖的RNA聚合酶(RNA-dependent RNA polymerase,RdRp)的原核表达载体,将重组表达载体转化大肠杆菌Rosetta,经异丙基硫代-β-D-半乳糖苷(Isopropylβ-D-1-Thiogalactopyranoside,IPTG)诱导获得RdRp基因原核表达蛋白.以重组蛋白为抗原免疫新西兰兔制备相应的多克隆抗体,Western blot检测表明,该抗体能与RdRp重组蛋白和感病中华蜜蜂幼虫蛋白特异性结合,说明获得的抗体特异性高.利用制备的抗体进行免疫荧光标记,发现其能特异地定位在感病中华蜜蜂血淋巴细胞内. 相似文献
69.
[目的]扩大鄂西地区稻瘟病菌菌库,分析其群体结构.[方法]收集鄂西不同地区2011年间的感病稻秆,分离保存稻秆上的穗颈瘟病菌,通过RAPD、rep-Pot2-PCR、REMAP 3种标记对分离的菌株进行分子标记.[结果]从9个稻秆上共分离得到29个稻瘟病菌.遗传多样性分析结果表明,相似性水平在75%时,2011年供试菌株可分为5个遗传宗谱,宗谱3(7个菌株)和4(19个菌株)为优势宗谱,其余3个为稀有宗谱.[结论] 2011年鄂西地区稻瘟病菌由5个遗传宗谱组成. 相似文献
70.