测定地克珠利混悬液中的聚山梨酯20含量和枸橼酸含量的方法     

李芳  孙立亚  杨丽英 《中国兽药杂志》2024,58(2):58-65

利用反相高效液相色谱法和蒸发光检测器快速便捷地测定地克珠利混悬液中聚山梨酯20和枸橼酸的成分及含量。吐温20方法：HPLC条件,Thermo U3000。流速：0.5 mL/min。流动相A:水,流动相B:乙腈。用梯度洗脱。ELSD 6000,漂移管温度：115℃。气体流速：2 L/min,稀释剂：乙腈。枸橼酸方法：橼酸的色谱条件：色谱柱：Tech Mate ST C18 250 mm×4.6 mm, 5μm,流动相：A;20 mmol磷酸氢二钠(用磷酸调节pH至3.0)B:色谱甲醇。流动相A∶B=95∶5。流速：1.0 mL/min,柱温：30℃紫外检测波长：UV 210 nm。该方法经过验证,有较好的精密度,线性及回收率等验证结果。该方法可以实现准确的定量分析,可作为一个可靠的检测方法。  相似文献   

Cucurbitacin B acts a potential insect growth regulator by antagonizing 20‐hydroxyecdysone activity          下载免费PDF全文

Chuanshan Zou  Guifen Liu  Suning Liu  Shumin Liu  Qisheng Song  Jian Wang  Qili Feng  Yunlin Su  Sheng Li 《Pest management science》2018,74(6):1394-1403

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1961~2010年云南省≥10℃活动积温时空变化格局     

王连超  郝成元 《河北农业科学》2016,(4):100-104

活动积温与植被演替、农作物生长关系密切,对区域生态状况和粮食安全具有决定性作用。基于GIS平台,研究了云南省31个气象站50 a的≥10℃活动积温时空分布格局及其变化特征。结果表明：（1）1961~2010年云南省≥10℃活动积温呈增长趋势,增幅为9.080℃/a,其中,1961~1978年≥10℃活动积温为负距平,1998~2010年为正距平,1979~1997年≥10℃活动积温距平以振荡为主;（2）5个代际≥10℃活动积温代际内最高值和均值随时间推移逐渐增大;（3）基本以景洪-思茅-元江-元谋为线,≥10℃活动积温向西、东2个方向逐渐减少;同时,空间差异程度随着时间推移有减小趋势。  相似文献   

miR-210-5p对南极独角雪冰鱼心脏发育的作用机制研究     

郭亚南  王丛丛  刘云  武晓会  孙建飞  陈程  许强华 《上海海洋大学学报》2016,25(4):481-487

microRNA为短链非编码RNA,通过与靶基因3'UTR序列互补在转录后水平发挥作用。已有研究表明,microRNA在心脏发育过程中起着重要的调控作用。南极冰鱼因体内缺乏功能性血红细胞,其心脏出现了补偿性增生。前期的研究提示,独角雪冰鱼心脏中特异表达的microRNAs可能与冰鱼心脏的补偿性增生相关。本研究针对南极冰鱼心脏中高表达的miR-210-5p,运用斑马鱼显微注射、靶基因预测等手段研究了miR-210-5p对心脏发育的作用机制。结果表明:斑马鱼胚胎注射miR-210-5p后,出现心包膜水肿,心脏发育畸形等现象。qRT-PCR分析显示,过表达miR-210-5p的斑马鱼胚胎中,心脏发育相关的标志性基因bmp4、smad1、gata6以及tbx2b的表达水平下调。Western blotting分析发现,bmp/smad通路中的BMP2、BMP4、SMAD1、GATA6以及TBX2B的蛋白表达水平也显著下调。通过对tbx20基因3'UTR的靶基因生物信息学预测,以及对其进行GFP荧光表达分析,发现tbx20基因可能是miR-210-5p的一个靶基因。由此推测,miR-210-5p可能通过抑制tbx20基因的表达,并调控bmp/smad通路以抑制冰鱼心脏发育。  相似文献   

Nutritional and health benefits of quinoa (Chenopodium quinoa Willd.)     

《Journal of Cereal Science》2016

Quinoa (Chenopodium quinoa Willd.) is a plant species of the Chenopodiaceae family, which originated in the Andean region and can adapt to different types of soil and climatic conditions. It is a pseudo grain with high nutritional value as it is rich in proteins, lipids, fiber, vitamins, and minerals, and has an extraordinary balance of essential amino acids. Quinoa also contains a high amount of health-beneficial phytochemicals including saponins, phytosterols, phytoecdysteroids. It is known that quinoa has considerably positive effects on metabolic, cardiovascular, and gastrointestinal health in humans. Despite all these health benefits, quinoa is not widely consumed due to several reasons, such as high import costs of the grain and lack of knowledge regarding its benefits among consumers. As we believe that further research is needed to provide more information about quinoa, this review was prepared to investigate its basic compounds and health effects.  相似文献   

Suitability of tef varieties in mixed wheat flour bread matrices: A physico-chemical and nutritional approach     

《Journal of Cereal Science》2015

Wheat flour replacement from 0 to 40% by single tef flours from three Ethiopian varieties DZ-01-99 (brown grain tef), DZ-Cr-37 (white grain tef) and DZ-Cr-387 (Quncho, white grain tef) yielded a technologically viable ciabatta type composite bread with acceptable sensory properties and enhanced nutritional value, as compared to 100% refined wheat flour. Incorporation of tef flour from 30% to 40% imparted discreet negative effects in terms of decreased loaf volume and crumb resilience, and increase of crumb hardness in brown tef blended breads. Increment of crumb hardness on aging was in general much lower in tef blended breads compared to wheat bread counterparts, revealing slower firming kinetics, especially for brown tef blended breads. Blended breads with 40% white tef exhibited similar extent and variable rate of retrogradation kinetics along storage, while brown tef-blended breads retrograded slower but in higher extent than control wheat flour breads. Breads that contains 40% tef grain flour were found to contain five folds (DZ-01-99, DZ-Cr-387) to 10 folds (DZ-Cr-37) Fe, three folds Mn, twice Cu, Zn and Mg, and 1.5 times Ca, K, and P contents as compared to the contents found in 100% refined wheat grain flour breads. In addition, suitable dietary trends for lower rapidly digestible starch and starch digestion rate index were met from tef grain flour fortified breads.  相似文献   

人工三倍体桑树新品种嘉陵20号的选育   总被引：12，自引：7，他引：5  

余茂德  敬成俊  吴存容  鲁成 《蚕业科学》2004,30(3):225-229

嘉陵 2 0号是用化学诱变与人工杂交相结合的染色体工程育成的人工三倍体桑树新品种 (2n =3x =4 2 )。母本为 792 0 (2n =2x =2 8) ,父本为人工四倍体西庆 4号 (2n =4x =5 6 )。该品种营养生长旺盛 ,枝条直立粗长 ,叶形大 ,叶肉肥厚 ,全年公顷产叶量比对照品种湖桑 32号 (2n =2x =2 8)增加 31 81%。养蚕试验万头蚕产茧层量比对照提高 5 84 % ,4 - 5龄蚕 10 0kg桑叶产茧量比对照提高 7 4 5 %。桑叶 (干物 )蛋白质含量 2 7 5 2 % ,比对照高 4 14百分点 ;可溶性糖含量 5 5 3% ,比对照高 1 0 4百分点。  相似文献   

Intra‐annual changes in reproductive indices of male and female Himalayan snow trout,Schizothorax richardsonii (Gray, 1832)     

Alexander Ciji  Prakash Sharma  Manchi Rajesh  Biju S. Kamalam  Annu Sharma  Pragyan Dash  M. S. Akhtar 《Aquaculture Research》2021,52(1):130-141

Periodic changes in reproductive hormone levels, gonadal histology and gonadosomatic index (GSI) of snow trout, Schizothorax richardsonii, were examined to ascertain annual cycle of gonadal development and reproductive status in their natural habitat. In females, there were coherent changes in plasma 17β‐oestradiol and vitellogenin along with GSI, oocyte maturation and vitellogenic progression, collectively indicating two distinct maturation peaks during the months of September and February. Coinciding with this, in males, plasma 11‐keto testosterone was also noticeably higher during September and February, with highest GSI values in September. However, plasma 17α, 20β‐dihydroxyprogesterone levels in males were found to be persistently high from September to February. This observation suggests the potential presence of matured oozing males over a longer period, unlike in females. Overall, the close association between reproductive hormone levels, GSI and gonadal maturation stages in males and females (particularly, the presence of postovulatory follicle complexes) with apparent natural synchronization clearly indicates that S. richardsonii breeds twice in a year, possibly during late September to early November and late February to early April in the coldwater riverine habitats of the Indian Himalayan region.  相似文献   

转基因枳橙中GA20ox1与rol基因互作关系的研究   总被引：2，自引：1，他引：1  

袁飞荣  邓子牛李芳  蒋巧巧Alessandra Gentile 严佳文 《中国农学通报》2011,27(19):214-221

为分析转rol ABC基因枳橙GA20ox1基因与rol基因表达的互作关系,进一步阐释其矮化性状形成的分子机制。以转rol基因枳橙实生苗为试验材料,研究其对赤霉素的敏感反应,用荧光定量RT-PCR分析GA20ox1基因和rol基因的表达,并检测幼芽中POD酶活性和植物内源激素含量的变化。结果表明转rol基因枳橙既不属于GA缺陷型,也不属于GA不敏感型,喷施GA3能促进其茎伸长生长,但恢复不到野生型水平,幼芽中IAA（P<0.01）、GA1和GA4（P<0.05）显著降低,POD酶活性显著提高（P<0.01）。转rol基因枳橙幼芽中GA20ox1基因mRNA水平相比对照显著下调（P<0.01）。B、D系与野生型嫩茎中无明显差异,B、D系老叶中明显降低,E系中嫩茎和老叶中均明显增加。B、D和E系嫩叶中GA20ox1基因转录表达均较野生型高。在幼芽、嫩茎中,rol C基因与GA20ox1表达负相关。rol基因通过在幼芽中的高表达下调了GA20ox1基因转录表达,进而抑制了活性GAs在幼芽中的合成,顶端分生组织较低量的活性GAs限制植物茎伸长,在转rol ABC基因枳橙矮化性状建成中发挥重要作用。  相似文献   

20％龙卷风水剂除治苗圃杂草药效试验   总被引：1，自引：0，他引：1  

金久宏 《西南林学院学报》2009,29(6):85-87

用20％龙卷风水剂进行除治苗圃杂草药效试验,结果表明：喷施20％龙卷风水剂200mL/667m^2后第7天,杂草除治效果为89．7％;喷施后第14天,杂草除治效果为95．6％;（杂）草鲜质量除治效果为96．1％,其药效要好于150mL／667m^2,与喷施20％克无踪水剂200mL／667m^2的药效相仿,且药效能持续20d,对苗木无影响,可在除治苗圃杂草上推广应用。  相似文献