鸡肉质鲜味特性相关基因的克隆及序列分析     

苏一军  季从亮  陈国宏  秦洁  束婧婷  张学余 《中国家禽》2005,9(Z1):6-8

本研究对泰和乌骨鸡、隐性白羽肉鸡腺苷琥珀酸裂解酶基因的cDNA进行了克隆,并对序列进行了分析,共发现有6处碱基突变249 G→A(TH)、717 C→G(TH)、985 A→G(TH)仅在泰和乌骨鸡中出现;992 T→C(RW)、1400 C→T(RW)仅在隐性白羽肉鸡中出现;而在泰和乌骨鸡和隐性白羽肉鸡中均检测到突变1179 A→C(TH,RW).其中985 A→G(TH)、1400C→T(RW)处突变导致两处氨基酸突变Thr→Ala(305)、Ala→Val(443),其它4处碱基突变均为同义突变.另外对几种脊椎动物Adsl基因核苷酸序列水平、蛋白质水平同源性进行了比较分析.  相似文献   

H5N1亚型禽流感病毒NS1基因的原核表达及其ELISA检测方法的建立   总被引：4，自引：0，他引：4  

杨涛刘明  张云刘春国  杜绍范 《中国兽医科技》2005,35(8):585-589

采用RT-PCR技术扩增了禽流感病毒（AIV）A／Goose／HLJ／p46／2003（H5N1）的NSl基因，将其克隆于融合蛋白表达载体pMAL-c2X上，转化DH5α大肠埃希氏菌感受态细胞，经BamHⅠ和HindⅢ双酶切鉴定及序列分析，表明筛选到了重组质粒pc2X-NS1。SDS-PAGE电泳结果显示，重组质粒转化TB1大肠埃希氏菌后，经0．3mmol／L的IPTG诱导，融合蛋白MBP-NS1得到大量表达，融合蛋白以可溶形式存在，分子质量约为67ku。Western-blotting检测结果表明，融合蛋白MBP-NS1能够与H5N1亚型AIV活病毒感染康复鸭血清发生特异性反应，而不能够与H5N1亚型AIV灭活疫苗免疫鸭血清发生反应。试验初步建立了以纯化的融合蛋白MBP-NS1为包被抗原的间接ELISA检测方法，为AIV灭活疫苗免疫家禽与AIV感染家禽的鉴别诊断奠定了基础。  相似文献   

鸡传染性腔上囊病病毒VP2基因在昆虫细胞中的表达   总被引：4，自引：0，他引：4  

李迎晓秦爱建  刘红梅邵红霞金文杰  刘岳龙 《中国兽医科技》2005,35(12):933-936

为了深入研究鸡传染性腔上囊病病毒（IBDV）VP2基因的结构和功能,利用Bac-to-Bac系统研制出含有VP2基因的重组杆状病毒rBac-VP2,将rBac-VP2感染Sf9细胞,并用抗IBDV VP2特异性单克隆抗体经间接免疫荧光试验检测,证实感染重组病毒Sf9细胞能高效表达IBDV VP2基因产物;Western-blotting分析结果表明,VP2基因表达产物的分子质量约为40 ku.  相似文献   

H7N2禽流感病毒分离株血凝素蛋白全基因的序列分析     

王传彬 孙明田克恭  金萍王宏伟  遇秀玲陈西钊 《中国兽医科技》2005,35(12):988-993

为进一步分析H7N2禽流感病毒（AIV）分离株血凝素（HA）基因的特性,参照已发表序列设计了1对引物,采用RT-PCR获得了1条约1.7 kb的DNA片段,测序后进行了同源性比较、HA基因系统发育进化树分析和氨基酸编码分析.结果表明,所测的2个分离株的HA基因全长1 664 bp,编码除信号肽以外的HA蛋白的全部544个氨基酸,其中包括HA1的323个氨基酸,HA2的221个氨基酸.2个分离株HA基因核苷酸序列的同源性为99.4%;与GenBank中AIV标准株A/Afri.Star./Eng-Q/983/79（H7N1）的同源性最高,分别为99.4%和99.0%;与美国A/Chicken/NewYork/13142-5/94（H7N2）株同源性很低（仅65.0%）,而与以色列、意大利H7N2 AIV的同源性较高（为96%～97%）;2个分离株在HA基因进化树中均处于H7亚型AIV的欧亚群系分支内.推导氨基酸的序列分析表明,其HA蛋白裂解位点的氨基酸序列为-GR-GLF-,仅包含1个碱性氨基酸（R-）残基,符合低致病力AIV的基因特征.  相似文献   

辣椒新品种镇江3号的选育     

马志虎  宋春 《辣椒杂志》2003,(3)

中晚熟杂交一代辣椒镇研3号是以江苏本地羊角椒自交系Y9007为母本,由甘肃酒泉引进的甜椒自交系T9218为父本配制的一代杂交组合。镇研3号为中熟辣椒品种,果形为牛角形,纵径15cm,肩横径5.0cm、肉厚0.40cm,单果平均重42g。果面光滑,青熟果绿色、味微辣,风味佳,适宜在长江流域或南方保护地栽培种植,667m2产量约为4200～5000kg。  相似文献   

珍黄88(湘椒29号)辣椒的选育     

马艳青  李雪峰  张竹青  陈文超  刘荣云  周群初  戴雄泽  邹学校 《中国蔬菜》2003,1(4):22-23

珍黄88的母本6427是从广西地方品种经自交纯化而成的优良黄皮尖椒自交系;父本8215为叶少、部分茎节短缩、肉厚疏松的辣椒高代自交系。该品种中熟,定植至采收青椒约50 d(天),产量2500～2800 kg·(667 m~2)~(-1),抗病毒病,中抗疫病,适于山东、广东等黄皮尖椒外运基地栽培;果实长牛角形,果表黄绿无皱,果型直,肉厚腔小,商品性好,耐贮运。  相似文献   

日光温室黄瓜济杂3号的选育   总被引：1，自引：1，他引：1  

孙汉友  赵殿国  于英俊  任国三  杨玉椿 《中国蔬菜》2003,1(4):34-35

母本88001来源于津杂2号,父本88010来源于津研2号与新泰密刺的杂交后代。其一代杂种济杂3号,瓜条顺直,长30～35 cm,单瓜质量约180 g,瓜把短,瓜皮深绿,密瘤白刺,品质优,商品性好,对霜霉病、白粉病、枯萎病抗性强。每667m~2产量7000～14000 kg,适合日光温室越冬茬和早春茬栽培。  相似文献   

春丰50苦瓜的选育     

刘政国  肖喜祝  李加振 《中国蔬菜》2003,1(6):19-20

以曼谷青皮、碧绿1号和钦州大肉苦瓜为育种材料,采用双父本混合花粉杂交和后代系谱选择育成优良自交系,再经配合力测定选出优良一代杂种春丰50。该品种生长势强,果实粗棒形,纵径26.3cm,横径6.45cm,皮色翠绿油亮,一般单果质量420g左右。平均每667m~2产量3 500kg。  相似文献   

Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats     

XU Rui  ZHANG Yun  ZHANG Mei  GE Zhi-ming  CHEN Wen-qiang  LI Xiu-chang  CAI Heng  ZHU Hui 《园艺学报》2003,19(5):642-644

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.  相似文献   

Quick detection of sex determining region protein gene in mouse fetuses     

《园艺学报》2003,19(5):622-626

AIM: To detect quickly the Y-chromosome specific sex determining region protein (Sry) gene in mouse fetuses on embryonic day 14.5 with a PCR method. METHODS: We designed specific primers with the OLIGO 5. 0 software. Templates were prepared in 30 minutes by the following way. About 1 mg embryonic tissue but not fetal liver was suspended, and treated with 200μL of lysis buffer, consisting of PCR buffer containing 20 mg/L proteinase K, 0. 5% NP-40, and 0.05% Tween 40, at 60°C for 15 minutes, heated for 5 minutes at 100 °C, 10μL was used as template. The PCR react ion was performed in 50μL, using two sets of primers specific for Sry gene (chromosome Y) and IL-3 gene (chromosome 11) . PCR conditions and cycle numbers were optimized. The assessment of the results was done by electrophoresis in 3% agarose run at high voltage. The specificity of the method was conf irmed by fluorescent in situ hybridization (FISH) using a specific male probe on embryonic tissue cells. RESULTS: Electrophoresis showed that PCR product of male control DNA consisted of a 649 bp product representing the IL-3 gene and a 444 bp product representing the Y-specific Sry gene, female control DNA only one 649 bp product. Fetuses with two bands matching those as seen inmale control DNA are the presumpt ive male fetuses. Fetuses, only the IL-3-associated 649 bp band, are the presumptive female fetuses. These were confirmed by FISH. The ent ire procedure took <3. 5 h. CONCLUSION: The established PCR assay offers a quick, simple, accurate, and sensitive detection of sex determining region protein gene in mouse fetuses. This method allowed the preparation and culture of pure male and female hematopoietic stem cells from fetal tissue.  相似文献