布鲁氏菌病微量补体结合试验的方法改进和应用     

蒋卉  冯宇  秦玉明  朱良全  范学政  丁家波 《中国农业科学》2022,55(8):1676-1684

[目的]补体结合试验是布鲁氏菌病(简称"布病")血清学确诊方法.现行国家标准《动物布鲁氏菌病诊断技术》(GB/T 18646-2018)中收录的补体结合试验分为常量法补体结合试验(CFT)和微量法补体结合试验(mCFT),但两者之间的相关技术参数和结果判定标准均存在差异,导致常量法CFT和mCFT检测结果不一致.笔者通...  相似文献   

仿刺参补体类似物活性的测定   总被引：2，自引：1，他引：1  

张峰  宫晶  王海峰  赵啸  李宣延 《大连水产学院学报》2007,22(4):246-248

用3种方法对仿刺参Apostichopus japonicus体腔液、体腔液上清液及体腔细胞中的总补体溶血活性和补体类似物AjC3、AjC4活性进行测定.结果表明:在仿刺参体腔液、体腔液上清液和体腔细胞中能够检测到总补体溶血活性和补体类似物AjC3、AjC4,表明仿刺参体内存在有脊椎动物的补体类似物,且活性与正常人补体活性相似.仿刺参体内总补体溶血活性和补体类似物AjC3、AjC4的活性依次顺序均为体腔液＞体腔细胞＞体腔液上清液.  相似文献   

Base-age invariant cork growth model for Spanish cork oak (<Emphasis Type="Italic">Quercus suber</Emphasis> L.) forests     

Mariola Sánchez-González  Isabel Cañellas  Gregorio Montero 《European Journal of Forest Research》2008,127(3):173-182

A cork growth model for Spanish cork oak forests was developed using data from 432 cork samples. Ten dynamic equations derived from the generalised algebraic difference approach (GADA) were considered for analysis, and both numerical and graphical methods were used to compare alternative models. All of the equations are base-age invariant and directly estimate accumulated cork thickness in complete years at any moment of the cork rotation. The fittings were done using the nested iterative method of the stochastic regression approach. The GADA formulation derived by Krumland and Eng (2005) from the Richards model by considering a ₁ and a ₃ as related to site productivity was finally selected. The cork growth model developed in this study allows the total accumulated cork thickness in complete years to be estimated in the fourth year of cork rotation with close to 80% reliability for any debarking period, increasing to nearly 90% from the eighth year onwards.  相似文献   

Induction of mRNAs in response to acclimation of trout cells to different osmolalities     

T. Sakamoto  N. Ojima  M. Yamashita 《Fish physiology and biochemistry》2000,22(3):255-262

The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A⁺) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.  相似文献   

中草药添加剂对生长肥育猪免疫功能的影响研究   总被引：18，自引：0，他引：18  

陈小波  田允波  葛长荣  高士争  韩剑众 《云南农业大学学报(自然科学版)》2002,17(2):176-179

 选用20 kg左右的“杜长大”三元杂交猪120头,分成对照组、中药组、中药+西药组,每组4个重复,进行饲养试验。每期实验结束前,每组采集8头猪血样,测定生长育肥猪血清中免疫球蛋白IgG,IgA,IgM和补体C₃,C₄的含量。结果表明:在20～30 kg,30～70 kg和70～110 kg阶段,与对照组相比,中药组、中+西组猪的血清IgG水平,分别提高了23.75%（P<0.05）和18.49%（P<0.05）,17.66%（P<0.05）和13.93%（P<0.05）,14.79%（P<0.05）和11.87%（P<0.05）;IgM水平,分别提高了25.04%（P<0.05）和14.99%（P<0.05）,22.81%（P<0.05）和15.18%（P<0.05）,5.79%（P>0.05）和4.23%（P>0.05）;IgA水平,分别提高了9.71%（P>0.05）和0.57%（P>0.05）,16.92%（P<0.05）和7.60%(P>0.05),15.01%（P<0.05）和7.23%（P>0.05）; C₃水平,分别提高了12.16%（P<0.05）和14.19%（P<0.05）,15.87%（P<0.05）和17.46%（P<0.05）,14.29%（P<0.05）和21.43%（P<0.05）;C₄水平,分别提高了13.51%（P<0.05）,5.02%（P<0.05）,6.67%（P<0.05）.研究结果提示,中草药添加剂对生长育肥猪体液免疫功能的影响,不仅促进免疫球蛋白的生成,而且还促进补体的生成,从而改善机体的体液免疫功能。  相似文献   

中南穿梭育种对南斯拉夫玉米群体改良的互补性研究     

谢振江  刘自华  张锦芬  Lazar Kojic 《北京农学院学报》2001,16(1):5-9

198 5- 1997年对南斯拉夫玉米群体SYN1/ 9C0 进行半同胞轮回选择。 1998年通过把在唐山C1～C6和在贝尔格莱德进行C3～C6共计 10个轮回群体与 4个标准自交系进行半双列杂交研究 ,连续 2年试验 ,结果一致。研究结果表明对群体产量和测交组合产量以及产量杂种优势、配合力和田间部分性状一般配合力均存在显著差异 ,南斯拉夫和唐山两地改良效果极具互补性。实验表明 ,中南穿梭育种在提高南斯拉夫群体自身产量、测交组合产量的同时 ,能更为有效地提升群体杂种优势 ,使产量特殊配合力、一般配合力同时提高  相似文献   

中国夏季旱涝预测研究-现状与进展     

蔡尔诚  唐浩 《黑龙江八一农垦大学学报》2002,14(1):1-5

根据作者提出的气候变化理论“冬季西风急流与夏季副热带高压的能量互补与平衡”对1997～2001年夏季旱涝进行了预测试验。5年的主要多雨带均与实际雨带吻合,准确率超过国内其他预报机构所作预报。  相似文献   

Fucoidan as a Potential Therapeutic for Major Blinding Diseases—A Hypothesis     

Alexa Klettner 《Marine drugs》2016,14(2)

Fucoidan is a heterogeneous group of sulfated polysaccharide with a high content of l-fucose, which can be extracted from brown algae and marine invertebrates. It has many beneficial biological activities that make fucoidan an interesting candidate for therapeutic application in a variety of diseases. Age-related macular degeneration and diabetic retinopathy are major causes for vision loss and blindness in the industrialized countries and increasingly in the developing world. Some of the characteristics found in certain fucoidans, such as its anti-oxidant activity, complement inhibition or interaction with the Vascular Endothelial Growth factor, which would be of high interest for a potential application of fucoidan in age-related macular degeneration or diabetic retinopathy. However, the possible usage of fucoidan in ophthalmological diseases has received little attention so far. In this review, biological activities of fucoidan that could be of interest regarding these diseases will be discussed.  相似文献   

单级单步多导数方法的代数稳定性     

张诚坚 《湖南农业大学学报(自然科学版)》1993,20(6)

本文从文献［１］中有关多值多导数方法的弱代数稳定性概念引伸出单级单步多导数方法的代数稳定性概念，并建立了若干单级单步多导数方法为代数稳定的充分条件与充要条件。  相似文献   

口蹄疫诊断技术研究进展   总被引：4，自引：0，他引：4  

彭贵青  陈焕春  钱平 《动物医学进展》2003,24(6):29-31

口蹄疫的有效控制关键在于早期检测 ,然而有很多疾病症状与口蹄疫相似 ,仅靠临床症状难以确诊 ,因此必须进行实验室诊断。实验室诊断包括病毒学诊断和血清学诊断。病毒学诊断方法有病毒分离、补体结合试验、酶联免疫吸附试验 ( EL ISA)、RT-PCR以及乳胶凝集试验 ( L AT)。 RT-PCR有待进一步完善 ,而用于野外检测的现场诊断方法已取得可喜进展。血清学诊断包括中和试验和 EL ISA,中和试验已经被 EL ISA方法取代 ,并且通过检测非结构蛋白的抗体可以区分感染动物和免疫动物。更加快速、敏感、可靠以及用于检测潜伏感染的诊断技术将是今后研究的热点。  相似文献