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81.
Vegetation indices are widely used as model inputs and for non‐destructive estimation of biomass and photosynthesis, but there have been few validation studies of the underlying relationships. To test their applicability on temperate fens and the impact of management intensity, we investigated the relationships between normalized difference vegetation index (NDVI), leaf area index (LAI), brown and green above‐ground biomass and photosynthesis potential (PP). Only the linear relationship between NDVI and PP was management independent (R2 = 0·53). LAI to PP was described by a site‐specific and negative logarithmic function (R2 = 0·07–0·68). The hyperbolic relationship of LAI versus NDVI showed a high residual standard error (s.e.) of 1·71–1·84 and differed between extensive and intensive meadows. Biomass and LAI correlated poorly (R2 = 0·30), with high species‐specific variability. Intensive meadows had a higher ratio of LAI to biomass than extensive grasslands. The fraction of green to total biomass versus NDVI showed considerable noise (s.e. = 0·13). These relationships were relatively weak compared with results from other ecosystems. A likely explanation could be the high amount of standing litter, which was unevenly distributed within the vegetation canopy depending on the season and on the timing of cutting events. Our results show there is high uncertainty in the application of the relationships on temperate fen meadows. For reliable estimations, management intensity needs to be taken into account and several direct measurements throughout the year are required for site‐specific correction of the relationships, especially under extensive management. Using NDVI instead of LAI could reduce uncertainty in photosynthesis models.  相似文献   
82.
This study aimed at elucidating SS-bonds of HMW-gliadins (HGL) from wheat with the focus on terminators of glutenin polymerisation. HGL from wheat flour extracts non-treated or treated with the S-alkylation reagent N-ethylmaleinimide (NEMI) were compared. HGL from wheat flour Akteur were isolated, hydrolysed with thermolysin and the resulting peptides pre-separated by gel permeation chromatography and analysed by liquid chromatography/mass-spectrometry using alternating electron transfer dissociation/collision-induced dissociation. Altogether, 22 and 28 SS-peptides from samples without and with NEMI treatment, respectively, were identified. Twenty-six peptides included standard SS-bonds of α- and γ-gliadins, high-molecular-weight and low-molecular-weight glutenin subunits. Eleven SS-bonds were identified for the first time. Fifteen peptides unique to HGL contained cysteine residues from gliadins with an odd number of cysteines (ω5-, α- and γ-gliadins). Thus, gliadins with an odd number of cysteines, glutathione and cysteine had acted as terminators of glutenin polymerisation. Decisive differences between samples without and with NEMI treatment were not obvious showing that the termination of polymerisation was already completed in the flour. The two HGL samples, however, were different in the majority of ten peptides that included disulphide-linked low-molecular-weight (LMW) thiols such as glutathione and cysteine with the former being enriched in the non-treated HGL-sample.  相似文献   
83.
The breadmaking quality of wheat is affected by the composition of gluten proteins and the polymerisation of subunits that are synthesised and accumulated in developing wheat grain. The biological mechanisms and time course of these events during grain development are documented, but not widely confirmed. Therefore, the aim of this study was to monitor the accumulation of gluten protein subunits and the size distribution of protein aggregates during grain development. The effect of desiccation on the polymerisation of gluten proteins and the functional properties of gluten were also studied. The results showed that the size of glutenin polymers remained consistently low until yellow ripeness (YR), while it increased during grain desiccation after YR. Hence, this polymerisation process was presumed to be initiated by desiccation. A similar polymerisation event was also observed when premature grains were dried artificially. The composition of gluten proteins, the ratios of glutenin to gliadin and high molecular weight-glutenin subunits to low molecular weight-glutenin subunits, in premature grain after artificial desiccation showed close association with the size of glutenin polymers in artificially dried grain. Functional properties of gluten in these samples were also associated with polymer size after artificial desiccation.  相似文献   
84.
Four sets of durum samples were used in this study to further understand the interrelationships among hard vitreous kernels (HVK), protein content, and pigment concentration, with a focus on the interaction and synergistic effects of protein content and vitreousness on durum quality. HVK level increases with higher protein content in the range of 9.5–12.5%, but this relationship is less evident in durum samples with high protein content (12.5–14.5%). Both protein content and kernel vitreousness can significantly affect durum milling quality. White starchy kernels (WSK) in low protein durum have a very detrimental impact on milling and pasta processing quality, but high protein content can mitigate the adverse impact of WSK on durum quality. Although protein content plays a dominant role, higher HVK might contribute positively to pasta firmness. There was no significant difference in yellow pigment content between HVK and WSK. However, pigment loss from semolina to dough was higher for WSK than HVK. Despite the difference in protein content, HVK and WSK have little difference in gluten strength. The monomeric protein was preferentially accumulated in HVK. The glutenin proteins of HVK and WSK were similar in the ratios of 1Bx/1By and HMW/LMW-GS.  相似文献   
85.
以番茄为试材,通过壳聚糖、可溶性淀粉、羧甲基纤维素钠配制成不同浓度的复合保鲜液浸泡涂膜处理,研究其对番茄保鲜效果的影响。结果表明,复合保鲜液涂膜可有效地降低番茄果实的失重率、腐烂率、可溶性固形物含量,抑制多酚氧化酶活性,维持番茄VC含量,保持番茄果实较好的感官品质,且以0.5%壳聚糖+0.8%可溶性淀粉+0.3%羧甲基纤维素钠复配的复合保鲜液涂膜对番茄有较好的保鲜效果,15℃环境下其货架期延长到15 d以上。  相似文献   
86.
潘葳 《中国农学通报》2017,33(14):130-137
旨在建立同时测定花生衣、葡萄皮、苜蓿、虎杖、藜芦等农产品中白藜芦醇含量的高效液相色谱标准化测定方法。色谱分析条件如下:以Waters Atlantis T3-C18色谱柱(4.6×150mm,3μm)分离,柱温40℃;以乙腈:0.1%乙酸溶液(体积比为26:74)为流动相,流速1.0 mL/min;采用二极管阵列检测器,检测波长306 nm。花生衣、葡萄皮、苜蓿中白藜芦醇含量的检出限均为0.6μg/g,定量限均为2.0μg/g;虎杖、藜芦中白藜芦醇含量的检出限均为7.5μg/g,定量限均为25μg/g。方法学考察结果显示符合含量测定要求。该方法操作简便、快速,结果可靠,重现性好,可用来研究农产品中白藜芦醇的含量。  相似文献   
87.
以北柴胡种子为试验材料,分别以0.5%、1.0%、1.5%和2.0%的酵母浸膏液对北柴胡种子进行浸种,在种子萌发过程中测定相关的生理生化指标,探讨酵母浸膏液对北柴胡种子萌发的影响.含水量的测定采用烘干减重法,体积的测定采用排水法,可溶性糖含量的测定采用蒽酮比色法,蛋白质含量的测定采用考马斯亮蓝-G 250染色法,氨基酸含量的测定采用茚三酮显色法;利用隶属函数法对各项指标进行综合评价.结果表明:北柴胡种子在萌发过程中,种子内含水量的变化和其体积的变化呈一定的相关性,都是呈先下降后上升再缓慢增加的趋势;可溶性糖含量的变化呈先下降后上升再下降的趋势;蛋白质含量整体呈先急剧下降后上升再缓慢下降的趋势;氨基酸含量整体呈先上升后下降的趋势.综合各项指标进行综合评价,酵母浸膏液浸种对北柴胡种子的萌发具有明显的促进作用,其中以1.0%酵母浸膏液浸种效果最佳.  相似文献   
88.
Recently, a new implement for controlling weeds in cereals (CombCut) has been developed. It cuts weeds in growing cereals without damaging them by using the physical differences (in height, stem thickness, straw stiffness and branching pattern) between crops and weeds. To evaluate and compare the effects of selective cutting with different timings of herbicide application on Cirsium arvense in spring barley, a randomised block experiment was conducted in Sweden in 2015–2017, in a field with a naturally occurring C. arvense population. Treatments consisted of control (C), herbicide application at 4–5‐leaf stage of C. arvense (H1), herbicide application at 8–10‐leaf stage (H2) and selective cutting at 10‐leaf stage (S). The treatments were performed in 2015 and repeated in 2016 in the same plots, and a final evaluation was performed in 2017. Compared to the control, S, H1 and H2 were equally efficient in reducing above‐ground biomass production of C. arvense and increasing spring barley grain yield per unit area. The number of C. arvense shoots per area was, however, higher in S compared to H1 and H2. No differences in control effects on shoot number were observed between H1 and H2. Our study indicates that (i) selective cutting (S) reduces C. arvense equally efficient as herbicide application and (ii) early herbicide spraying is as efficient as spraying later in the season.  相似文献   
89.
5种杀虫剂超低容量液剂对玉米田草地贪夜蛾的防治效果   总被引:1,自引:0,他引:1  
草地贪夜蛾是新入侵我国的迁飞性重大害虫,为害玉米的心叶、生长点及雄穗等,能引起严重的损伤、烂心、授粉不良等,导致玉米减产甚至绝产。本文评价了5种杀虫剂超低容量液剂通过低容量喷雾对玉米田草地贪夜蛾的防治效果。结果表明,在相同的原药成本下,6%甲维·茚虫威超低容量液剂和1%甲氨基阿维菌素苯甲酸盐超低容量液剂具有优异的保叶效果和杀虫效果,5%氯虫苯甲酰胺超低容量液剂在较低的用量下(有效用量1 g/667m~2)具有良好的保叶和杀虫效果,3%呋虫胺超低容量液剂对草地贪夜蛾幼虫基本无效,但有一定的保叶效果。  相似文献   
90.
基于人参样品基质特点,采用快速滤过型净化法 (m-PFC) 结合高效液相色谱-串联质谱 (HPLC-MS/MS),建立了人参中嘧菌酯、苯醚甲环唑、吡虫啉、茚虫威和噻嗪酮5种农药残留同时检测的分析方法。该方法具有较低的检出限 (0.03~0.18 μg/kg) 和定量限 (0.11~0.59 μg/kg),其定量限较中国国家标准GB 2763—2016中规定的对人参中苯醚甲环唑和嘧菌酯的最大残留限量 (MRL) (0.5~1 mg/kg) 低3个数量级,5种农药的线性范围在1~500 μg/L之间。在2、10、50、100和300 μg/kg的添加水平下,方法的平均回收率均在76%~115%之间,相对标准偏差 (RSD) 均小于11%,回收率较好且方法稳定。与传统样品前处理方法相比,该方法具有操作简单、省时省力和灵敏度高等优点。将该方法应用于市售6份人参样品中5种农药残留的检测,结果未检出相应的农药残留。该方法为研发建立更快速、准确地检测人参中农药残留的方法提供了思路,也为后续中国国家标准修订人参中农药最大残留限量提供了参考。  相似文献   
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