宁夏乳制品产业在夹击中崛起的对策思考     

武荣 《农业科学研究》2010,31(1):69-73

中国入世后乳制品产业逐步放开,国际品牌对国内乳业存在潜在威胁.宁夏乳制品产业要在伊利、蒙牛、光明等国内强势产业的夹击中发展与崛起,必须知己知彼,认清自己的优势,找准突破方向.在精确定位消费群体、不断调整产品结构、加强品牌建设、走集团化发展之路、建设人才培养工程等方面狠下工夫,才能使宁夏乳制品企业步入健康发展的快车道.  相似文献   

Insight into the Meligethes aeneus voltage‐sensitive sodium channel structure and an attempt to select the best pyrethroid ligands          下载免费PDF全文

Przemysław Wieczorek  Barbara Wrzesińska 《Pest management science》2016,72(1):162-171

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Differential mechanisms of action of the novel γ‐aminobutyric acid receptor antagonist ectoparasiticides fluralaner (A1443) and fipronil          下载免费PDF全文

Miho Asahi  Masaki Kobayashi  Hiroto Matsui  Kunimitsu Nakahira 《Pest management science》2015,71(1):91-95

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Retarding effect of simvastatin on artery remodeling induced by high-salt and high-fat diet in rats     

YIN Ming-jing  WEN Han-chun  YE Yong-wei  GUAN Qing-lin  HUANG Hao-zhang  HUANG Lu-shuang  ZHU Ji-jin 《园艺学报》2012,28(2):228-233

AIM: To investigate the effect of simvastatin intervention on the changes of blood pressure, serum lipid fluctuation and aortic configuration induced by high-sodium and high-fat diet in rats. METHODS: Sixty adult male SD rats were randomly divided into 5 groups (n=12): control (N)group, high salt (S)group, high fat (F) group, high salt+ high fat (SF) group and high salt+high fat + simvastatin (T) group. After fed for 16 weeks, the rats were subject to determine blood pressures and serum concentrations of triglycerides (TG),total cholesterol(TC) and soluble CD40 ligand (sCD40L). The expression of CD40/CD40L in the root of ascending aorta was detected by immunohistochemical method. The thickness of intima media in the ascending aorta as well as the ratio of lumen area/total vascular area were measured and calculated after HE staining. RESULTS: In S group, F group and SF group, systolic blood pressure was significantly higher than that in N group (P<0.01). Systolic blood pressure in T group were slightly higher than that in N group with statistical significance and significantly lower than that in SF group. The serum concentrations of TG and TC in F group and SF group were significantly higher than those in N group and T group (P<0.01), and no significant difference among S group, N group and T group was observed. In S group, F group and SF group, the serum concentrations of sCD40L were higher than that in N group and T group (P<0.05), meanwhile that in SF group was also higher than that in S group and F group (P<0.05). However, no significant difference of sCD40L concentration between S group and F group as well as N group and T group was observed. The expression of CD40/CD40L in the ascending aorta in S group, F group and SF group was higher than that in N group and T group (P<0.05), and that in SF group was also higher than that in S group and F group (P<0.05).No significant difference of CD40/CD40L expression between S group and F group as well as N group and T group was observed. The thickness of intima media in S group, F group and SF group was significantly thicker than that in N group (P<0.01), and no significant difference of the intima media thickness between T group and N group was observed. The ratio of lumen area/total vascular area in S group, F group and SF group was smaller than that in N group (P<0.05), and no significant difference of the ratio between T group and N group was found. CONCLUSION: Feeding high-fat and high-salt diet leads to blood pressure elevation, induces atherosclerosis, increases serum concentration of sCD40L and enhances the expression of CD40/CD40L in arterial tissues. The combination of the stimuli has stronger effect than a single factor. Statins protect the arterial tissues against atherosclerosis by decreasing the level of serum sCD40L and inhibiting the arterial expression of CD40/CD40L.  相似文献   

A G326E substitution in the glutamate‐gated chloride channel 3 (GluCl3) of the two‐spotted spider mite Tetranychus urticae abolishes the agonistic activity of macrocyclic lactones          下载免费PDF全文

Sven Geibel  Thomas Van Leeuwen 《Pest management science》2017,73(12):2413-2418

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Cytotoxicity of TRAIL against several tumor cells     

ANG Lei  YANG Lian-ping  LI Ru-bing  KONG Xiang-ping  PANG Jian-xin 《园艺学报》2007,23(6):1236-1237

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Preparation of recombinant human soluble TRAIL and its inducing effect on apoptosis of tumor cells by TRAIL combined with bortezomib     

LI Xue-yan  XU Xia 《园艺学报》2015,31(11):1933-1942

AIM: To construct a prokaryotic expression plasmid to produce recombinant human tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and to verify the biological activity of TRAIL. METHODS: The prokaryotic expression plasmid pET-28a (+)-TRAIL_114-281 was constructed. Human soluble TRAIL was obtained through optimized inducing protein expression and purification conditions. The biological activity of TRAIL was verified by CCK-8 assay. The apoptosis-inducing effect of TRAIL alone and/or in combination with proteasome inhibitor bortezomib (Velcade, PS-341) on the tumor cell lines H460(TRAIL-sensitive) and K562(TRAIL-resistance) for 24 h was determined. The apoptotic rates of the cells were analyzed by flow cytometry with Annexin V-FITC/PI staining. The activities of caspase-8, -9 and -3 in the cells were detected by colorimetric method. The protein expression of Bax, Bcl-2 and cFLIP was measured by Western blot. The expression of DR4 and DR5 in the H460 cells and K562 cells after treated with bortezomib for 24 h was detected by flow cytometry. RESULTS: The recombinant human soluble TRAIL protein with stable bioactivity was successfully acquired, which induced apoptosis in H460 cells and K562 cells. After treatment with different concentrations of TRAIL, the apoptotic rate of H460 cells was significantly increased with the increase in the concentration of TRAIL (P<0.05), but the apoptotic rate of K562 cells was not affected by the increasing TRAIL concentration. Apoptotic rate in combination group was obviously higher than that in single group (P<0.05). In the process of apoptosis, the activities of caspase-8, -9 and -3 in H460 cells and K562 cells were both increased. The expression of Bcl-2 and cFLIP in treatment groups (especially the combination group) was decreased compared with control group. No significant change of the Bax expression level was observed. The expression of DR4 and DR5 in the H460 cells and K562 cells was significantly up-regulated after treated with bortezomib (P<0.05). CONCLUSION: Bortezomib combined with recombinant human soluble TRAIL synergistically induces apoptosis in tumor cell lines H460 and K562 through initiating intrinsic apoptotic pathways by up-regulating death receptors DR4 and DR5, and reducing the expression of antiapoptotic proteins Bcl-2 and cFLIP.  相似文献   

A New Binding Model for Structurally Diverse ALS Inhibitors     

Toshio Akagi 《Pest management science》1996,47(4):309-318

Acetolactate synthase (ALS) has been a very attractive target for herbicides for the last decade. There are several ALS inhibitors in commercial use spanning the world-wide market. In this study, the common structural features within a subset of ALS inhibitors were investigated by molecular graphics and quantum chemical calculations. Satisfactory results were obtained with model calculations based on the presumption that the relative location of the inhibitor azine moiety and some receptor cationic group remained fixed. The cationic group was assumed to interact with an acidic group in each of the inhibitors. This model explains many aspects of ALS inhibitors (sulfonylureas, triazolopyrimidines, pyrimidyl ethers and other classes) such as: (1) the common structural feature among the different classes of ALS inhibitors, (2) the substituent effects in the hydrophobic moiety of each class and (3) the structure–activity relationships of the acidic moiety of each class. These are significant achievements for a model based on in-vivo herbicidal activity and gas-phase calculations, but the model also has its limitations: (1) Only compounds with acidic groups and azine moieties can be addressed, (2) the structure–activity relationships of the hydrophobic moiety are not yet fully understood and (3) only a qualitative prediction of activity levels is possible. A preliminary trial of ligand design predicted the novel skeleton of an ALS inhibitor that was published independently from this study.  相似文献   

Assessment of neuropeptide binding sites and the impact of biostable kinin and CAP2b analogue treatment on aphid (Myzus persicae and Macrosiphum rosae) stress tolerance     

Lucy Alford  Richard Marley  Anthony Dornan  Jean‐Sbastien Pierre  Julian AT Dow  Ronald J Nachman  Shireen A Davies 《Pest management science》2019,75(6):1750-1759

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Formulation of a first-order kinetic model and release of added phosphorus in a savanna soil     

Nafiu Abdu 《Archives of Agronomy and Soil Science》2013,59(1):71-81

Adsorption–desorption of added phosphorus (P) was studied in a batch experiment using anion-exchange resin. Total P sorbed by adding 400 mg P kg^?1 by Nigerian soil ranged from 10.8 mg kg^?1 in the Idofian Basement complex to 35.5 mg kg^?1 in Alkaleri Sandstone, representing 3 and 9% of applied P. Phosphorus release kinetics was apparently described by the first-order, second-order, Elovich, parabolic diffusion and fractional power equations, but not in soils derived from sandstone. The mechanism underlying the release pattern was concluded to be dissolution followed by diffusion of sorbed P from the good fit to the Elovich and parabolic diffusion equations. The inability to clearly relate the P sorbed by the soil to OH^- and SO₄ ^2- released into the soil solution during the adsorption process further corroborated the above conclusion, thereby overruling the possibility of ligand exchange as a dominant mechanism in the sorption/desorption of P in these soils.  相似文献