In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes   总被引：1，自引：0，他引：1  

Momena Khatun  Mohammad Musharraf Uddin Bhuiyan  Jalal Uddin Ahmed  Aminul Haque  Mohammad Bozlur Rahman  Mohammed Shamsuddin 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):75-82

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle''s salt with L-glutamine and sodium bicarbonate) for 27 h at 39℃ under 5% CO₂ in humidified air. The oocytes retrieval and efficiency (mean ± SD) per prepubertal and pubertal goats were 5.2 ± 0.6 and 6.8 ± 0.6, and 77.3 ± 0.1% and 80.5 ± 0.6%, respectively. Anaphase I - telophase I stages differed significantly (7.3 ± 0.8 vs. 2.6 ± 0.2, p < 0.05) between the two groups of goats. After IVM, the percentages of metaphase II were significantly higher (66.3 vs. 60.3, p < 0.05) in pubertal goats than in their prepubertal counterparts. The percentages of normal in vitro fertilization (IVF) in Fert-Tyrode''s albumin lactate pyruvate of pubertal goat oocytes did not differ between Percoll and swim-up sperm separation methods (36.7 ± 0.9% vs. 32.7 ± 1.3%, p > 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production.  相似文献   

甲氧基丙烯酸酯类杀菌剂复配专利技术现状分析   总被引：1，自引：0，他引：1  

陈翠翠  梁艳辉 《安徽农业科学》2015,(19)

从复配对象、申请量年代分布、申请区域分布、申请人分布等对国内外包含甲氧基丙烯酸酯类化合物相关的复配专利进行检索分析,指出了该领域的研究焦点和方向,并提出了国内相关领域机构或企业的研究方向及建议.  相似文献   

SpringerLink电子期刊全文数据库的检索研究     

李玲  杨桂珍 《农业图书情报学刊》2005,17(1):84-86,88

文章论述了SpingerLink电子期刊全文数据最新版本的检索功能和检索方法。  相似文献   

网络环境下文献检索课教学创新研究——以广州大学为例     

孟齐霞  熊伟华 《农业图书情报学刊》2012,(8):134-136,146

在网络信息化潮流的推动下,图书馆的文献检索课教学经历着一场深刻变革,文献检索课课程设置必须适应信息化的发展趋势,提高学生对文献检索课的学习兴趣,文献检索课必须进行全方位改革。从课程设置适应信息化发展要求,从文献检索课教学方法、教学手段和教学模式等方面提出了文献检索课程改革的方向,拟解决网络环境下文献检索课的教学问题。  相似文献   

美国图书情报学院的《信息检索》课程     

张娟  胡德华 《农业环境科学学报》2012,31(4)

调研了美国图书馆学会认可的图书情报学院开设《信息检索》课程情况,如开设该课程的院校、课程名称、课时、课程内容、面向对象等,分析了我国《信息检索》课程教学的现状,针对问题提出了改进建议。  相似文献   

文献调研在医学科研中的作用     

王萧  程瑾  周培岚  杨晓茹  王磊 《农业环境科学学报》2012,31(10)

 以医学科研中遇到的实际问题为例,详细分析了文献调研在选题、实验设计、实施实验、分析结果和撰写总结报告5个阶段中的不同作用。  相似文献   

6株肠艾美耳球虫繁殖力的比较研究     

孙秀梅  田培荣  方素芳  顾小龙  崔平 《安徽农业科学》2012,(19):10151-10152,10177

[目的]为肠艾美耳球虫的早熟选育及兔球虫疫苗的研制提供理论依据。[方法]采用6个不同地区肠艾美耳球虫的孢子化卵囊经口接种45日龄无球虫兔,接种剂量为1×104个卵囊/只,并对6株肠艾美耳球虫的繁殖力进行比较。[结果]衡水株排卵量最多,张家口株次之,胶南株排卵量最少。睢宁株的排卵量接近胶南株,如皋株、通江株相近,在感染后第8.5～9天均有排卵,各虫株的排卵高峰期相似,第13天排卵量达到峰值,第21天接近0。[结论]不同地理株肠艾美耳球虫的排卵量不同,但排卵高峰相一致。  相似文献   

基于改进方向纹理谱特征的图像检索     

毋小省  孙君顶 《勤云标准版测试》2012,32(4):812-818

在定义图像局部邻域纹理方向特性的基础上,提出了一种新的方向纹理谱描述符。该描述符针对局部邻域内中心像素与其相对的邻域像素,既充分考虑了它们间的灰度变化关系,又考虑了它们间灰度差异的变化关系,从而更有效地描述了局部纹理特征。为证明新描述符的分辨能力,采用4种不同图像库进行图像检索对比实验,结果表明,本文的新纹理谱描述符取得了最好的检索效果。  相似文献   

咸宁桂花品种分类调查研究     

王振启  陈洪国 《北方园艺》2012,(17):101-105

根据刘玉莲制定的桂花5级分类标准,对咸宁桂花进行了调查。结果表明:咸宁市桂花包括4个品种群,即四季桂品种群、银桂品种群、金桂品种群和丹桂品种群。四季桂品种群有9个品种,其中当地品种5个,引入品种4个;银桂品种群有19个品种,其中当地品种15个,引入品种4个;金桂品种群有18个品种,其中当地品种12个,引入品种6个;丹桂品种群有16个品种,其中当地品种7个,引入品种9个。根据以上调查结果,编制了咸宁桂花品种分类检索表,为充分挖掘咸宁桂花品种资源提供参考。  相似文献   

From Experience-Oriented to Quantity-Based: A Method for Landscape Plant Selection and Configuration in Urban Built-Up Areas     

Heyi Wei  Zhengdong Huang 《Journal of Sustainable Forestry》2013,32(8):698-719

Sunshine is the key ecological factor for the growth, development, and reproduction of all green plants. Experience-oriented methods are the main approach in the stage of plant selection and configuration for landscape planning and design. However, our survey showed that this approach is unreliable in complex built-up environments. The objective of this research was to find a sound strategy for the selection and configuration of landscape plants, thereby ensuring their healthy growth and development. In this research, we adopted a geographic information system (GIS) as the technological platform, combined with a sunshine simulation and evaluation model, and a landscape plant database. The system can automatically retrieve and match landscape plants for different levels of solar radiation at specific geographic sites. This quantity-based assessment of the solar environment can overcome the drawbacks of the experience-oriented approaches, and can be used for landscape plant selection in the early stages of landscape planning and design, and also for their conservation and replacement.  相似文献