Effect of Aurora protein kinase inhibitor VX-680 on homogeneity adhesion and migration in human hepatoma HepG2 cell     

REN Ben-hong  SUN Xue-jiao  HAO Yue-peng  KOU Jun-ting  NIU Shi-shi  YANG Cheng-yuan  WANG Xiao-xia 《园艺学报》2018,34(5):945

AIM:To study the effect of Aurora protein kinase inhibitor VX-680 on homogeneous adhesion and migration ability in human hepatocellular carcinoma cell line HepG2. METHODS:The HepG2 cell were divided into experimental group and control group, respectively. VX-680 was used in experimental groups at 3 concentrations (3.125 μmol/L group, 6.25 μmol/L group and 12.5 μmol/L group). DMSO was used in the control group. The effects of VX-680 at different concentrations on the adhesion ability of human hepatocellular carcinoma HepG2 cells were observed by cell slow aggregation test and separation experiment. The effects of VX-680 at different concentrations on the migration ability of HepG2 cells was detected by wound healing assay. The expression of E-cadherin in HepG2 cells was detected by Western blot. RESULTS:The results of the slow aggregation test showed that compared with the control group, the number of cell clumps formed in experimental groups was significantly decreased (P<0.01). The results of separation experiment showed that the ratio of N_TC/N_TE gradually decreased with the increased concentration of VX-680. The results of wound healing assay showed that as the concentration of VX-680 increased, the cell scratch healing ability gradually weakened compared with control group. The results of Western blot showed that the protein expression of E-cadherin in the HepG2 cells increased with the increased concentration of VX-680 (P<0.05). CONCLUSION:VX-680 increases the homogeneous adhesion and inhibits the migration of HepG2 cells.  相似文献   

基于离散元的不同轴截面形状下沙柳颗粒致密成型研究     

李震  王宏强  高雨航  闫莉  王鹏  德雪红 《林产工业》2019,46(2):14-18,44

为研究模具的结构形状对沙柳颗粒致密成型的影响,用离散元软件EDEM建立了沙柳颗粒模型,并将预先用Pro/E创建的3种不同结构的模具先后导入其中,在初始参数、条件设定完成后分别做了匀速压缩模拟。对比研究了3种模具下压缩力的做功,颗粒在竖直和水平方向的形变特征、受力过程,并通过后处理所得数据分析计算了各模具下成型燃料最终获得的密度和能量。结果表明:双弧度、双锥度、单锥度3种模具下压缩过程做功依次为48.86、56.93、66.02 J,燃料总能量分别是40.06、41.54、46.81 J,对应密度为1.23、1.19、1.17 g/cm3;竖直方向双弧度下燃料内部颗粒受到的接触力和发生的形变远大于其他两种模具,水平方向三者颗粒所受的接触力和发生的形变无太大差异;颗粒在3种模具下受到的粘接力均无明显不同,但各自所受的法向粘接力远大于切向粘接力。  相似文献   

炎症反应与白细胞迁移   总被引：2，自引：0，他引：2  

孙鹂 《安徽农业科学》2011,39(21):12863-12865,12905

人体自我防御中,白细胞定向迁移是先天性免疫必不可少的步骤。然而白细胞在创伤部位的募集也导致了炎症反应的发生。白细胞的移动和它周围微环境的变化精确地调控着一系列级联事件。趋化因子在白细胞迁移过程中起着重要的作用,对介导细胞黏附和迁移的趋化因子的深入研究将有助于对疾病病理状态的了解和控制。  相似文献   

Effects of Chlamydia pneumoniae infection on adhesion and migration of vascular smooth muscle cells     

WANG Bei-bei  ZHANG Li-jun  QUAN Wei  SHEN Bing-ling  ZHANG Teng-teng  ZHANG Li-jun 《园艺学报》2010,26(12):2289-2294

AIM: To investigate the effects of Chlamydia pneumoniae (C.pn) infection on the adhesion and migration of vascular smooth muscle cells (VSMCs). METHODS: Primary VSMCs, isolated from the aorta of SD rats, was infected with C.pn after the culture and propagation of C.pn in the HEp-2 cells. The morphological characteristics of C.pn inclusions in VSMCs were examined under the fluorescence microscope with acridine orange (AO) staining. The specific DNA fragment of C.pn was identified by polymerase chain reaction (PCR). Cell adhesion assay was performed to investigate the effect of C.pn infection on the adhesion of VSMCs to collagen I. Wound-healing assay and Transwell assay were performed to detect the effects of C.pn infection on VSMCs migration. RESULTS: The C.pn inclusions and infection spots were observed in VSMCs under fluorescence microscope with AO staining. The inclusions were larger than infection spots in volume, but were much lower in count. A 437 bp-specific fragment of C.pn DNA was detected in C.pn-infected VSMCs by PCR. In the cell adhesion assay, the absorbance values in C.pn infection group were far higher than those in control group 2 h after infection (P<0.01). The cell adhesion ratio in C.pn infection group was 134.38%. The migration distance of VSMCs infected with C.pn was significantly longer than that of the control cells 24 h after infection by a wound-healing assay (P<0.05). More migratory VSMCs infected with C.pn for 24 h were found in a Transwell assay than those in control group (P<0.01). CONCLUSION: C.pn infection significantly promotes the adhesion and migration of VSMCs.  相似文献   

SigmaB基因缺失对单核细胞增生性李斯特菌毒力的影响     

杨丽红  孟庆玲  乔军  才学鹏  张再超  蔡扩军  王为升  王俊伟  陈创夫 《西北农业学报》2013,22(8):92-97

为了解缺失SigmaB基因对单核细胞增生性李斯特菌（LM）的毒力影响。通过体外生长试验、巨噬细胞RAW264.7的粘附与侵袭试验、小鼠肝脾载菌量试验、毒力基因转录水平试验、小鼠LD₅₀试验来研究缺失株（LM XS5 △SigmaB）的毒力变化。结果显示,与野毒株相比,缺失株体外生长能力减弱、侵袭率和粘附率降低,小鼠肝脾载菌量显著减少,毒力基因（inlA、inlB、hly、prfA、actA）的转录水平降低,而小鼠LD₅₀升高。可见,SgmaB基因对LM的毒力具有重要的调控作用,为LM基因缺失疫苗和活疫苗载体的研发可资借鉴。  相似文献   

几何非光滑典型生物体表防粘特性的研究   总被引：14，自引：7，他引：14  

任露泉  丛茜 《农业机械学报》1992,23(2):29-35

研究表明,几何非光滑体表是土壤动物减粘降阻的原因之一。通过对典型土壤动物蜣螂的大量观察、实验研究和表面分析,发现其体表不同部位具有不同的几何非光滑;将土壤力学与生态学相结合,还探讨了在粘湿环境中蜣螂非光滑体表与不同触土方式的关系及其对防粘特性的作用机理。  相似文献   

The role of CD44 adhesion factor in canine mammary carcinomas     

Madrazo J  García-Fernández RA  García-Iglesias MJ  Durán AJ  Espinosa J  Pérez-Martínez C 《Veterinary journal (London, England : 1997)》2009,180(3):371-376

CD44 is an adhesion molecule implicated in the progression of human breast cancer. The purpose of this study was to describe CD44 antigen expression in canine mammary carcinomas and to evaluate its prognostic significance in relation to other clinico-pathological variables.A reduction in CD44 expression was significantly related to variables such as tumour size and adherence to underlying tissues but was not related to tumour location or to ulceration of the overlying skin. Complex (grade I) and anaplastic (grade III) carcinomas exhibited more intense expression of this antigen than did some tubulopapillary and most solid carcinomas (grade II). Although reduced CD44 expression was associated with infiltrative growth and vascular invasion in solid carcinomas, intense expression was also observed in anaplastic tumours. Although overall these findings suggest a role for this adhesion factor in canine mammary tumour development and progression, the complexity and apparently paradoxical nature of some of the findings currently limit the use of this immunohistochemical marker as a prognostic indicator.  相似文献   

Role of Laparoscopy in Diagnosis and Management of Equine Colic     

《Veterinary Clinics of North America: Equine Practice》2023,39(2):339-349

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