Study of microRNA Expression Profile in Different Regions of Ram Epididymis     

Cuiling Wu  Chunxin Wang  Bo Zhai  Yunhui Zhao  Zhuo Zhao  Zhiyu Yuan  Mingxin Zhang  Kechuan Tian  Xuefeng Fu 《Reproduction in domestic animals》2021,56(9):1209-1219

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Sperm production and quality in European eel (Anguilla anguilla) in relation to hormonal treatment     

Paraskevas Koumpiadis  Daniela E. Sganga  Sebastian N. Politis  Victor Gallego  Ian A. E. Butts  Juan F. Asturiano  Ioannis E. Batjakas  Jonna Tomkiewicz 《Reproduction in domestic animals》2021,56(12):1497-1505

Aquaculture production relies on controlled management of gametogenesis, especially in species where assisted reproduction is needed for obtaining gametes in captivity. The present study used human chorionic gonadotropin (hCG) treatments to induce and sustain spermatogenesis in European eel (Anguilla anguilla). The aim was to evaluate effects of strip-spawning timing (12 vs. 24 hr) after weekly administration of hCG and the necessity of a primer dose (in addition to weekly hormonal treatment) prior to strip spawning (primer vs. no-primer) on sperm quality parameters. Sperm parameters included milt production (weight), density and sperm kinematics at Week 9, 11 and 13 after onset of treatment. Spermiation commenced in 11.5% of males in Week 5 and by Week 9, and all males produced milt. Male weight, milt production, sperm density and spermatocrit did not differ among hormonal treatments during the experimental period. Overall, male weight decreased from 106.3 to 93.0 g, milt weight increased from 3.5 to 5.4 g, sperm density counts decreased from 11.7 × 10⁹ to 10.5 × 10⁹ cells/ml, and spermatocrit decreased from 46.5% to 40.5%. Furthermore, spermatocrit was positively related to haemocytometer counts (R² = .86, p < .001), providing a reliable indicator of sperm density. Differences in sperm kinematics were observed depending on strip-spawning timing after hormonal injection (12 vs. 24 hr) but with no consistent pattern. These sperm quality parameters also did not consistently differ between the no-primer and primer treatments. Considering that each male may be stripped 4–5 times over the 2–3 months spawning season, omitting the primer would reduce animal handling, material costs and labour intensity, while sustaining high-quality sperm production.  相似文献   

离心除精浆对提高冷冻精液品质的作用     

姬云涛屈长青  曹斌云 《中国农学通报》2005,21(11):9-12,32

采用离心洗涤法去除精浆后,对精液进行冷冻处理。发现:1、除精浆精子冻后的活率、Ⅰ类顶体率和精子畸形率与未除精浆组存在显著差异(P<0．05);2、除精浆使稀释后精液精浆中GOT和LDH活性明显升高(P<0．05)。从平衡后到解冻后各组GOT和LDH活性都呈上升趋势。但除精浆试验组比未除精浆试验组上升缓慢;3、除精浆试验组精子的穿卵率、卵内平均精子数都显著高于未除精浆试验组(P<0．05);4除精浆离心洗涤液用生理盐水和脱脂乳-卵柠液．两者之间无显著差异(P>0．05)。  相似文献   

Advances in Collection,Transport and Maturation of Equine Oocytes for Assisted Reproductive Techniques     

《Veterinary Clinics of North America: Equine Practice》2016,32(3):379-399

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稀释液中添加海藻糖对猪冷冻精液质量参数和活性氧的影响   总被引：8，自引：2，他引：8  

金一  陈宠霞  方南洙  金英海 《中国畜牧杂志》2005,41(10):6-8

本试验旨在探讨海藻糖对猪精子冷冻效果以及精子内活性氧(ROS)的含量影响。试验分对照组和4个海藻糖处理组(0.025,0.05,0.1和0.2 mol/L)。精子冷却后,添加0.1 mol/L海藻糖处理组精子活力显著(P<0.05)高于对照组,而精子内和稀释液中各组间ROS发生量都没有显著的差异。精子冷冻解冻后,添加0.05 mol/L海藻糖处理组的精子活力显著(P<0.05)高于对照组;添加0.05 mol/L和0.1 mol/L海藻糖处理组的精子生存率显著(P<0.05)高于对照组;添加0.1 mol/L海藻糖处理组顶体完整的精子百分比显著(P<0.05)高于对照组;4个海藻糖处理组膨胀精子百分比都显著(P<0.05)高于对照组;添加0.025 mol/L海藻糖处理组ROS水平显著(P<0.05)低于对照组。结果表明:海藻糖对精子冷冻保存是有益的,且能抑制精子内ROS的发生,但它的作用机制有待于进一步的研究。  相似文献   

中草药复方添加剂对繁殖后期种公鸡精液品质及种蛋受精率的影响   总被引：3，自引：0，他引：3  

秦绪光  刘福柱 《中国饲料》2007,(16):22-25

选用80只50周龄尼克T种公鸡随机分成Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ组,每组16只。Ⅰ、Ⅱ组为对照组,分别饲喂基础日粮和基础日粮添加鸡蛋,Ⅲ、Ⅳ、Ⅴ组为试验组,分别饲喂基础日粮中添加1%、2%、3%中草药复方添加剂的日粮,研究中草药复方添加剂对繁殖后期种公鸡精液品质及种蛋受精率的影响。结果表明:日粮中添加中草药复方添加剂能显著提高种公鸡的精液品质和精子活力,降低精子畸形率,并且能提高种蛋受精率和健雏率,其中2%添加组效果最明显。  相似文献   

精子受精抗原-1(FA-1) mRNA在绵羊睾丸和附睾中的表达   总被引：1，自引：0，他引：1  

娜仁花  旭日干 《畜牧与兽医》2006,38(11):1-3

本研究通过RT-PCR检测了该基因在绵羊睾丸和附睾中的表达情况。首先,提取绵羊睾丸组织、附睾头、体、尾部组织总RNA,以此为模板,反转录合成cDNA,自行设计引物,PCR扩增出462 bp的目的DNA。然后,将目的片断克隆入T载体,通过菌液PCR和重组质粒酶切,鉴定重组质粒中的目的DNA。再经序列分析鉴定目的片断。同时,以β-actin为内参照物,进行RT-PCR半定量分析,比较精子受精抗原(FA-1)在睾丸、附睾头、体、尾组织中的表达量。结果表明,FA-1在绵羊睾丸和附睾中均表达。  相似文献   

Haplotype analysis of β-actin gene for its association with sperm quality and boar fertility     

C.-L. Lin  D.G.J. Jennen  S. Ponsuksili  E. Tholen  D. Tesfaye  K. Schellander  & K. Wimmers 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(6):384-388

β‐actin (ACTB) was examined as a direct functional candidate gene for the possible association with sperm concentration, motility (MOT), semen volume per ejaculate, plasma droplet rate, abnormal sperm rate (ASR) and the fertility traits, non‐return rate and number of piglets born alive (NBA). Three polymorphisms in intron 3 (T>C) and one polymorphism in exon 4 (T>C) of porcine ACTB gene were identified by comparative sequencing of animals of the breeds Pietrain and Hampshire. Association analysis revealed that haplotypes affected the variation of the traits MOT, ASR and NBA. The beneficial haplotypes may provide considerable improvement of sperm quality and fertility in the tested commercial boar population.  相似文献   

附睾功能及其对精子成熟的影响   总被引：1，自引：2，他引：1  

李琳  周利华  任冬仁  幸宇云  陈从英 《中国畜牧兽医》2007,34(1):79-82

哺乳动物的附睾为精子的成熟提供了特殊的微环境，是精子成熟、保护、运输和贮存的部位，在生殖过程中起着重要作用。附睾上皮细胞的合成、分泌、转运等功能使精子发生了一系列结构、生化和功能的改变．从而使精子获得受精和运动能力。作者对附睾的形态结构、功能和对精子成熟影响研究现状等作简要综述。  相似文献   

动物性别决定的分子机理及性别鉴定与控制新技术   总被引：12，自引：0，他引：12  

段子渊 《中国牛业科学》1998,24(3):52-55

哺乳动物中位于Ｙ染色体短臂临界区域的ＳＲＹ基因启动雄性性状的发育。该基因在生殖腺脊中的表达可激发下游基因ＭＬＳ的转录,引起缪氏体抑制物和睾酮分泌,促使睾丸组织器官的发育。针对该基因制备特异探针或产该区域片段得到引物对就可用ＦＩＳＨ或ＰＣＲ准确,快速鉴定出植入母体前的胚胎性别,以及对精子筛选分离的结果作出准确评价。与ＰＣＲ相比,ＦＩＳＨ技术更具检测优势。  相似文献