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Growth and habitat of spotted halibut Verasper variegatus in the shallow coastal nursery area, Shimabara Peninsula in Ariake Bay, Japan 总被引：2，自引：0，他引：2
Toshihiro Wada Naoki Mitsunaga Hiroyuki Suzuki Yoh Yamashiya Masaru Tanaka 《Fisheries Science》2006,72(3):603-611
ABSTRACT: To determine the growth performances and habitat characteristics of spotted halibut Verasper variegatus , successive samplings were conducted in the south-eastern coastal area of Shimabara Peninsula in Ariake Bay. The push-net samplings were performed at Kamaga beach from May 2003 to April 2004 to determine the horizontal distribution of larvae and juveniles, while the gill net samplings were done in the coastal waters of Tatsuishi beach from July 2003 to July 2004 to collect the larger juveniles and adults. A total of 478 individuals from metamorphosing larvae to 2-year-old fish (15.2–447.0 mm total length [TL]) were sampled. In particular, the 2003-year class fish were successively collected from May 2003 to June 2004 (15.2–350.0 mm TL; n = 418) and the mean total length in March, June, September, December 2003 and May 2004 was 22.4, 82.5, 172.5, 203.9 and 296.4 mm, respectively. The present study found that metamorphosing larvae migrated into the estuarine tidal flats during March 2003; newly settled juveniles inhabited the shallow intertidal areas; and juveniles had a remarkably high growth in shallow coastal areas. It is suggested that young fish around 300 mm TL migrated from the nursery grounds in Ariake Bay to deeper waters around Tachibana Bay during May–June 2004. 相似文献
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圆斑星鲽的人工繁殖及育苗技术 总被引：6，自引：0，他引：6
本文报道了圆斑星鲽的人工繁殖、育苗过程及结果，并比较了培育温度对仔、稚鱼生长和成活率的影响等。使用自然海区捕获的亲鱼，注射HCG，在水温14℃条件下，效应时间为24-28hrs。受精卵在水温14℃条件下，经94-96hrs孵出。仔鱼经55天培育，共培育出平均全长23．3mm的幼鱼5.5万尾，其成活率为18．3％。 相似文献
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[目的]了解红尾副鳅生物学性状及人工驯养的可行性。[方法]测定了366尾野生红尾副鳅的生物学性状,并对其人工驯养进行研究。[结果]红尾副鳅平均体长为(122.89±2.58)mm,平均体重为(13.04±0.62)g。红尾副鳅体长与体重的关系式为:y=0.000 2x2.279 5(R2=0.795 2)。雌性红尾副鳅的绝对怀卵量为162～756粒,平均怀卵量为(437.3±31.27)粒。红尾副鳅的怀卵量与体重的关系式为y=29.814x+8.038 3(R2=0.682 6)。红尾副鳅的怀卵量与体长的关系式为y=0.003 9x2.378(R2=0.702 2)。雌性红尾副鳅初次性成熟年龄为2龄,雄性在1龄时就有成熟个体出现,繁殖旺季为4～5月。在人工驯养条件下,红尾副鳅能摄食人工配合饲料,且生长速度较快。经60 d饲养,红尾副鳅的增重率和成活率分别为27%～32%和78%～97%。[结论]该研究可为开发红尾副鳅为新养殖对象积累资料。 相似文献
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María Del Mar Ortega-Villaizán Romo Shigenori Suzuki Masamichi Nakajima Nobuhiko Taniguchi 《Fisheries Science》2006,72(1):33-39
ABSTRACT: The barfin flounder has been recently declared to be rare, as the number of wild individuals in Japan has greatly reduced since 1975. The Japanese National Center for Stock Enhancement started the stocking program for this species on 1987. In the present study, microsatellite DNA loci were applied in order to determine the pedigree of the hatchery-produced juveniles for stock enhancement. Additionally, using six polymorphic microsatellite markers, the accuracy to assign dyads to categories of kinship was estimated using the relatedness estimator ( R XY ), similarity indexes (individual genetic identity [GI] and proportion of shared alleles [ P S ]), identification of kin groups using unweighted pair group method with arithmetic mean (UPGMA) clustering based on interindividual genetic distances (1- R XY , 1- P S , and 1-GI), a likelihood ratio approach, and partition methods to separate individuals into cohorts. The results were compared with the pedigree relationship previously obtained from parentage analysis. Estimation of the kinship relationship between individuals could be better inferred by means of UPGMA dendrogram clustering based on 1-GI or 1- P S as interindividual genetic distances, or using computer software to perform partitioning of set of individuals. 相似文献
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感应流速是鱼类游泳特性的关键指标之一,获取鱼类的感应流速将有助于过鱼设施低流速区的流速设计和增值放流地点的选择等。本研究以黑水河的红尾副鳅（Paracobitis variegates）、横纹南鳅（Schistura fasciolatus）和山鳅（Oreias dabryi）为研究对象,利用固定流速法对其感应流速进行测试。结果显示,3种鳅科鱼类的绝对感应流速范围为5.7-13.2 cm/s,相对感应流速的范围为0.6-1.5 BL/s。红尾副鳅的绝对感应流速显著高于横纹南鳅和山鳅,相对感应流速显著低于横纹南鳅和山鳅（P<0.05）,横纹南鳅和山鳅无显著差异（P>0.05）。所有测试鱼的绝对感应流速和相对感应流速均与体长呈显著相关关系,绝对感应流速随体长的增加而增加,相对感应流速随着体长的增加而减少。体长可能是影响3种鳅科鱼类感应流速差异的主要原因。所有测试鱼的趋流率与流速呈指数函数关系为PR=97.93e^(〖-e〗^(-0.91V+6.34) )。本研究结果可为金沙江流域鱼类行为学研究提供基础资料,为黑水河过鱼设施设计和增值放流地点选择提供参考和依据。 相似文献
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本研究采用RACE末端扩增方法,得到全长为3872 bp的圆斑星鲽(Verasper variegatus) piwil2基因序列,命名为Vvpiwil2,开放阅读框(ORF)长为3192 bp,编码1063个氨基酸,5?-UTR和3?-UTR的长度分别140 bp和540 bp。基于ExPASy、SMART、Signal4.1和NCBI的保守结构域(CDD)数据库在线分析对蛋白序列结构进行预测,推断Vvpiwil2编码的氨基酸分子量为118.6 kDa,理论等电点为9.02,无跨膜结构及信号肽,有3个结构域：ArgoL1结构域、PAZ结构域及PIWI结构域。利用实时荧光定量PCR技术对圆斑星鲽不同发育时期的胚胎、仔稚鱼以及雌雄成鱼的不同组织表达模式进行分析。结果显示,Vvpiwil2基因从胚胎发育早期至高囊胚期均大量表达,之后呈下降趋势,直至孵化阶段。由于胚胎从卵裂至囊胚时期的发育过程主要受细胞质成分引导,直至原肠早期,mRNA开始大量转录合成,实现由母源型向合子型的过渡,推断Vvpiwil2是母源性基因。孵化后仔稚鱼68 d时,Vvpiwil2基因表达量显著高于其他时期,表明Vvpiwil2的功能可能与圆斑星鲽性腺分化过程相关;Vvpiwil2基因在雌雄成鱼性腺中的表达量显著高于其他组织,且卵巢中的表达量显著高于精巢,推测Vvpiwil2基因在卵巢功能的维持中发挥重要作用。本研究结果为解析圆斑星鲽性别决定机制提供了新的靶标基因,为建立全雌化苗种繁育技术打下坚实的理论基础。 相似文献