GnRH analogue treatment on LH surge day 0 followed by single transvaginal artificial insemination with frozen semen on day 5 in bitches     

Tadatoshi OHTAKI  Yasuna KOGA  Mamiko ONO  Gen WATANABE  Kazuyoshi TAYA  Shigehisa TSUMAGARI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):123-126

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外源激素对草鱼垂体GTH细胞影响的比较研究     

鲁双庆 《湖南农业大学学报(自然科学版)》1995,(3)

通过对草鱼脑垂体ＧＴＨ细胞（促性腺激素分泌细胞）的组织化学和超微结构观察，比较研究了注射外源神经激素（ＬＲＨ－Ａ）和外源促性腺激素（ＰＧ）对ＧＴＨ细胞的影响及作用途径。结果证实：ＬＲＨ－Ａ刺激垂体ＧＴＨ细胞产生促性腺激素，ＧＴＨ细胞内分泌颗粒（ｇｒ）大量减少，分泌小球（ｇｌ）彼此融合后解体，垂体组织ＧＴＨ细胞间出现空腔，有部分ＧＴＨ细胞解体。ＬＲＨ－Ａ作用途径是通过垂体ＧＴＨ细胞起作用；ＰＧ不通过垂体组织起作用，注射ＰＧ以后，ＧＴＨ细胞不发生变化。  相似文献   

Molecular characterization,tissue distribution of Follicle‐Stimulating Hormone (FSH) beta subunit and effect of kisspeptin‐10 on reproductive hormonal profile of Catla catla (Hamilton, 1822)          下载免费PDF全文

Mohd Ashraf Rather  Irfan Ahmad Bhat  Niti Sharma  Rupam Sharma  Gireesh‐Babu P  Aparna Chaudhari  Jeetendra Kumar Sundaray 《Aquaculture Research》2016,47(7):2089-2100

Gonadotropin (GTH) hormones are glycoprotein which stimulates gonadal maturation in vertebrates. Follicle stimulating hormone is involved in initiation of gametogenesis and regulation of gonadal growth. FSHβ has been cloned and characterized from the brain of Catla catla. The FSHβ full‐length of cDNA sequence of 523 bp comprised 3, 394 and 128 bp of 5′‐UTR, open reading frame (ORF) 3′‐UTR respectively. The coding region of C. catla FSHβ encoded a peptide of 130 amino acids. Phylogenetic analysis of C. catla FSHβ deduced amino acid sequence showed high similarity with Gobiocypris rarus followed by goldfish, Carassius auratus. The qPCR result shows that FSHβ mRNA is mainly expressed in pituitary while moderate and low expression was observed in testis and ovary respectively. Chitosan‐nanoconjugated kisspeptin‐10 (CK‐10) of particle size 125 nm, polydispersity index of 0.335 to 0.65 and zeta potential of ?34.95 mV were synthesized and evaluated at against naked kisspeptin‐10 for their reproductive hormonal profile. Treatment of fish with CK‐10 showed controlled and sustained surge of the reproductive hormones (FSH & LH) with peak at 12 h. The hormone levels of naked kisspeptin‐10 treated fish decline after 6 h. The sustained release of this CK‐10 will help in reducing maturation age, synchronization of ovulation and spawning in fish. This is the first report on use of chitosan‐nanoconjugated kisspeptin‐10 (CK‐10) for reproduction in fish.  相似文献   

LHRH-A、DA对施氏鲟脑垂体碎片释放GtH的影响     

胡红霞  张勇  朱华  李立成  姚志刚 《水产学报》2014,38(3):378-384

为了探讨在古老的软骨硬鳞鱼中促性腺激素(GtH)的双重内分泌调节作用,本实验设计用离体灌流的方法研究促黄体素释放激素类似物(LHRH-A)和多巴胺(DA)对施氏鲟脑垂体碎片分泌GtH的影响。引入10、100和1 000 nmol/L 3个浓度的LHRH-A对施氏鲟脑垂体碎片3次脉冲式刺激实验;每次间隔1 h,持续5 min,研究不同剂量LHRH-A对鲟鱼脑垂体释放GtH的作用;用200 nmol/L DA对施氏鲟脑垂体碎片持续2 h灌流后引入5 min的1 000nmol/L LHRH-A刺激实验,研究DA如何抑制鲟鱼脑垂体释放GtH。每5 min收集一管灌流液,用放射免疫测定法(RIA)检测灌流液中GtH的含量。结果显示,低剂量LHRH-A随着刺激引入脑垂体释放GtH出现波浪式的增加,中、高剂量出现释放延后现象。LHRH-A在10nmol/L到1 000 nmol/L范围内对刺激脑垂体释放GtH没有剂量依存关系。DA对施氏鲟脑垂体碎片GtH的分泌没有显著影响,但是可以抑制LHRH-A引起的GtH分泌,即DA不能抑制施氏鲟GtH的基础分泌,而只能抑制LHRH-A诱导的GtH分泌。研究结果证明,在高等硬骨鱼类中存在的双重神经内分泌调节在古老的鲟鱼中也存在。  相似文献   

GnRH类似肽主动免疫对公猪生产性能的影响     

方富贵  章孝荣  汪宏云  吴义师  周俐兵  陶应乐 《动物医学进展》2005,26(2):64-66

将30头公猪分成3组,给免疫组的 10头公猪注射促性腺激素释放激素并列体二 聚体 卵清蛋白复合物,初次免疫时间是3月 龄,5月龄加强免疫1次。同时阉割10头公 猪作为阴性对照组,其余的10头注射卵清蛋 白作阳性对照组。试验期间,统计每头公猪 的平均饲料消耗量和日增重。7月龄时屠宰, 测其活重、屠宰率、料肉比指标。结果表明, 免疫组公猪平均日增重、最终活重(P< 0.05)、屠宰率、料肉比均高于阴性对照组,略 低于阳性对照组。提示GnRH并列体二聚体主动免疫能提高公猪的生产性能。  相似文献   

Spawning induction and seed production of Eastern little tuna,Euthynnus affinis (Cantor, 1849), in the post‐ and pre‐spawning seasons by hormonal treatment in a semi‐closed recirculation system with elevated temperature          下载免费PDF全文

Ryosuke Yazawa  Yutaka Takeuchi  Kenta Satoh  Yuri Machida  Kotaro Amezawa  Naoki Kabeya  Goro Yoshizaki 《Aquaculture Research》2017,48(7):3472-3481

We previously established a method for spawning induction in Eastern little tuna (ELT) Euthynnus affinis (Cantor, 1849) by administering a gonadotropin‐releasing hormone analog (GnRHa) during the natural spawning season in Japan (August–October). In order to establish seed production of ELT in the off‐spawning season, we first conducted three spawning induction trials by GnRHa administration from October 2011 to January 2012 using ELT broodstock (2 years old; three females and four males) maintained in a 10‐m³ tank with a semi‐closed recirculation system and static elevated temperature. Average water temperature and daily egg production in three trials lasting 11–15 days were 27.0 ± 0.09°C and 268 173 eggs (Trial 1), 27.0 ± 0.11°C and 277 9098 eggs (Trial 2), and 25.5 ± 0.39°C and 291 113 eggs (Trial 3) respectively. Mean fertilization rate and mean hatching rate were 70.4% and 60.5% (Trial 1), 83.9% and 79.6% (Trial 2), and 62.5% and 57.4% (Trial 3) respectively. We also succeeded in producing ELT larvae in the pre‐spawning season (April–July), although the quantity and quality of larvae produced were inferior to those produced in other calendar months. In trials involving periodic GnRHa administration during the off‐spawning seasons, hatched larvae were obtained in the 10‐m³ tank after six of nine administrations in the 2011–2012 off‐spawning season and in 16 of 19 administrations in the 2012–2013 off‐spawning season. The findings of this study demonstrated that hormonal treatment and thermal control could be used to extend the spawning period in ELT, potentially allowing larval production in the post‐ and pre‐spawning seasons.  相似文献   

绵羊GnRHR基因部分序列PCR-SSCP分析   总被引：2，自引：0，他引：2  

刘忠慧  储明星  陈国宏  方丽  叶素成 《安徽农业大学学报》2006,33(3):318-321

采用PCR-SSCP技术,用两对引物分析了GnRHR基因外显子2和外显子1部分序列在小尾寒羊、多赛特羊2个绵羊品种中的多态性.结果表明,对于引物1扩增片段,2个绵羊品种均只检测到AA基因型.对于引物2扩增片段,2个绵羊品种均检测到AA、AB基因型;小尾寒羊AA、AB基因型频率分别为0.83和0.17,多赛特羊AA、AB基因型频率分别为0.60和0.40.引物2的多态片段测序分析表明,位于GnRHR基因cDNA第230处发生了碱基的改变(G→C),该突变导致了氨基酸的改变(甘氨酸→半胱氨酸).  相似文献   

促性腺激素释放激素对兔雌性生殖系统发育的影响   总被引：1，自引：0，他引：1  

杜惜明  文兴豪  李子义 《中国兽医学报》2000,20(5):508-510

将 2 1只处于间情期的雌兔 ,随机分成试验组和对照组 ,试验组每只肌注 1 m L( 1 5μg)促性腺激素释放激素 ,然后分 7个阶段取材观察卵巢和子宫的形态学变化。结果表明 ,注射促性腺激素释放激素可促进兔卵泡发育、成熟及排卵 ,加速黄体形成 ,促进子宫内膜增殖和内膜腺的发育  相似文献   

GnRH脉冲对体外原代培养大鼠GTH细胞中FSHβ mRNA表达水平的影响   总被引：3，自引：0，他引：3  

王新  谭建华  赖小平  万忠海  韦旭斌 《动物医学进展》2011,32(4):55-58

为了从分子水平阐述不同脉冲频率促性腺激素释放激素(GnRH)对促性腺激素(GTH)分泌促卵泡素(FSH)的影响,采用细胞体外培养技术结合荧光定量PCR技术,研究体外培养大鼠原代垂体细胞在不同脉冲频率GnRH刺激下,细胞FSHβ mRNA的变化.在相同振幅条件下,低频刺激(120 min间隔)时,FSHβ mRNA表达水...  相似文献   

Steroidogenic capacity of coho salmon ovarian follicles throughout the periovulatory period     

Dr Glen Van Der Kraak  Edward M. Donaldson 《Fish physiology and biochemistry》1986,1(4):179-186

Coho salmon follicles obtained at various times throughout the periovulatory period were incubatedin vitro with graded amounts of partially purified salmon gonadotropin (SG-G100) for 24 h and the amounts of 17β-estradiol, testosterone and 17α20β dihydroxy-4-pregnen-3-one (17α20βP) released into the media determined by radioimmunoassay. By this approach, the pattern of steroid secretion by ovarian follicles was shown to change in relation to the developmental status of the oocyte. Full-grown immature follicles produced large amounts of 17β-estradiol but negligible amounts of testosterone and 17α20βP. Both basal and gonadotropin-stimulated 17β-estradiol production was subsequently reduced with advancing oocyte development. In contrast, the production of testosterone and 17α20βP increased during the course of ovarian development with testosterone production highest in follicles with a peripheral germinal vesicle and 17α20βP production highest in matured and postovulatory follicles. These data are discussed in relation to information on the preovulatory changes in circulating levels of steriod hormones in salmonids.  相似文献