枸橼种质对柑橘溃疡病的抗性鉴定     

朱志媚  谭李梅  徐静  符红艳  胡哲  龚蕾  杨贵兵  王萍  马先锋  邓子牛 《中国果树》2021,(1):43-49,110,111

柑橘溃疡病(Xanthomonas citri subsp. citri,Xcc)是严重阻碍柑橘产业发展的世界性检疫病害,防控困难,选育抗病品种为防治溃疡病的唯一有效办法。枸橼C-05是柑橘属独特的抗溃疡病种质,为抗性机理和抗病育种提供了种质基础。为了进一步评价枸橼C-05的抗性,另外收集了13份枸橼种质,对其进行溃疡病的离体和活体抗性评价。叶片形态学观察表明,14份枸橼种质存在较大的形态学差异。采用离体与活体注射接种不同浓度的柑橘溃疡病菌,根据叶片的感病症状、病斑周围黄色晕圈大小等典型溃疡病症状,综合评价枸橼类种质的抗病性。结果表明:枸橼种质之间对溃疡病的抗性存在极大差异,溃疡病的抗性评价综合病情指数显示枸橼C-05、园香橼、矮果香橼、美国枸橼、普通枸橼和印度大果综合病情指数较小,表现为极抗病或抗病,其他枸橼种质表现不同程度的感病性;枸橼种质的抗病或感病性与其叶片形态特征无关。枸橼C-05和5个抗病枸橼种质对柑橘溃疡病具有针对性的抗性,这对进一步研究抗病机理和抗病育种具有重要意义。  相似文献   

品质不同的稻米胚乳细胞形态特征的扫描电镜观察   总被引：5，自引：0，他引：5  

瞿波  徐运启 《华中农业大学学报》1991,10(4):404-408,T002

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甘蓝型油菜两种纯合中芥酸基因型的分离和鉴定   总被引：3，自引：0，他引：3  

刘定富  周永明  刘后利 《华中农业大学学报》1988,(3)

本研究从中国高芥酸甘蓝型油菜品种华油8号与加拿大无芥酸品种Altex杂交的后代中,分离和鉴定出了两种不同的纯合中芥酸基因型,它们在一个基因座位上具有纯合的有芥酸基因E.在另一座位上具有纯合的无芥酸基因c,其基因型组成可分别用E_AE_Ae_Ce_C和e_Ae_AE_CE_C表示。二者的芥酸含量均为27%左右,基本上等于双亲的平均值。这两种基因型的杂交后代和回交后代的分离结果表明,其遗传行为与无芥酸和高芥酸杂交后代的结果相同,这再次证实了芥酸的遗传假说,同时也证实了这两种基因型的遗传组成。文后讨论了这两种基因型的可能用途。  相似文献   

小菊花色芽变品种的AFLP分析     

秦贺兰  贾宗锴  张西西 《河北林果研究》2010,25(4):337-340

采用AFLP(Amplified Fragment Length Polymorphism)技术对小菊花色芽变品种与原始品种的DNA进行差异性分析,旨在建立芽变品系形态学以外的分子水平鉴定技术。在引用13对引物中,每对引物平均扩增出13.5条多态性带,分子量在110～800bp之间。3对引物组合(第8对E-ACG/M-CAT、第10对E-AGG/M-CTC、第11对E-AGG/M-CTG)产生5条清晰特异性条带可以将二者区分,这些特异性片段可能包含引起花色芽变的基因序列,经计算,二者遗传相似系数为0.986,遗传物质多态性为2.81%。  相似文献   

Effect of Aquasorb and Organic Compost Amendments on Soil Water Retention and Evaporation with Different Evaporation Potentials and Soil Textures     

《Communications in Soil Science and Plant Analysis》2012,43(13-14):2031-2055

Abstract

Aquasorb PR3005A, a hydrophilic polymer (a salt copolymer polyacrylamide), and garden waste compost were added to a loamy sand and a loam soil in pots to assess their impact upon soil physical properties at two different evaporation potentials. Compost was mulched and incorporated, the Aquasorb was incorporated, and their effect on temperature and amelioration of soil water content and evaporation was investigated. Mulching with compost reduced evaporation and increased soil temperature. Maize (Zea mays var. single cross 704) was sown in the same pots later, and growth indicator factors (plant height, fresh and dry weight, root weight, and leaf area) were compared. It was concluded that compost mulch application is beneficial to soil water retention whereas compost incorporation did not show these benefits. Compost mulch advances seedling emergence and enhances early growth through hydrologic soil amelioration. High rates of Aquasorb were also beneficial in advancing the emergence and early growth of maize seeded in loamy sand. The hydration capacity of Aquasorb is reduced as the electrolyte concentration and electrical conductivity are increased. Increased electrolyte concentration in soil solution, through drying, may result in gel dehydration and water release at potentials greater than field capacity, which may be lost to drainage. Furthermore, it is concluded that pot experiments with amendments fail to simulate field conditions.  相似文献   

Comparison of physiological and yield traits between purple- and white-pericarp rice using SLs     

Zhi-Juan Ji  Xiao-Guang Wang  Yu-Xiang Zeng  Liang-Yong Ma  Xi-Ming Li  Bing-Xin Liu  Chang-Deng Yang 《Breeding Science》2012,62(1):71-77

Five physiological and eleven yield traits of two pairs of sister lines generated from a high generation with similar genetic background (SLs) for purple pericarp were investigated to explore the reasons behind low-yield production of colored rice. Of the five physiological traits examined, except grain anthocyanin content, there were generally similar trends between the P (purple-pericarp) lines and the corresponding W (white-pericarp) lines over two seasons (in the year 2009 and 2010 separately). The results demonstrated that the chlorophyll content of flag leaves, the net photosynthetic rate of flag leaves, and the grain anthocyanin content could be easily influenced by the environment. The physiological functions of the traits for the P lines were more active than those of the corresponding W lines in the year 2010. The grain anthocyanin content of the P lines was much greater in the year 2010 than in the year 2009 during the growth period. The investigation of yield traits revealed that the P lines had reduced 1000-grain weight, yield per plot and grain/brown rice thickness compared to the W lines. A difference comparison of these traits and a source-sink and transportation relationship analysis for these SLs suggested that small sink size was a key reason behind yield reduction of purple pericarp rice.  相似文献   

棉花皱缩花叶病的初步研究     

章松柏  张长青  吴祖建  谢联辉 《河南农业科学》2010,(3)

2007年湖北荆州地区一些棉田棉花叶片上发生一种新的棉花病害,暂定名为棉花皱缩花叶病。通过症状描述、电镜观察和生物学接种对其进行了初步分析。结果显示:该病害症状疑似病毒病,但与已经报道的棉花病毒病症状都不相同。电镜观察显示,病株叶片汁液中存在4种杆状病毒颗粒,大小分别约是18nm×300nm、18nm×500nm、18nm×800nm、18nm×1100nm。生物学接种结果表明,该病不能经种子、棉蚜、烟粉虱、汁液摩擦4种方式传播。  相似文献   

云南橡胶树气刺微割采胶新技术研究应用进     

李明谦 《热带作物学报》2010,30(8):63-67

详细介绍了云南植胶区进行气刺微割采胶新技术试验的研究内容及应用实施情况，通过4 a的微割试验示范研究，比较系统的分析了云南微割采胶试验的产量效应、干含、割胶速度、耗皮量、死皮性状、净增长率等生产性数据。说明了微割采胶新技术具有操作简单、割胶速度快、产量好、节约原生树皮、死皮较轻等特点，并能显著降低割胶劳动强度，有效的提高割胶劳动生产率。全面的掌握了这一新技术的应用原理、实施技术、材料配用、操作规程、安全风险，积累了良好气刺微割推广应用基础。  相似文献   

Effects of Brown Planthopper,Nilaparvata lugens (Stl) (Homoptera: Delphacidae), Feeding on Callose Deposition in Rice with Different Tolerance     

YANG Li  CHEN Jian ming  ZHANG Hai qiang  ZHANG Jue feng  HE Yue ping 《中国水稻科学》2013,27(6):624

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某引水隧洞围岩变形探讨     

王荣杰  彭良余 《林业科技情报》2012,(3):144-145,148

某引水隧洞主要采用双护盾式TBM施工。在穿越高埋深的区域性断裂带时,采用绕行导坑人工钻爆法施工。本文对采用绕行导坑的围岩变形情况进行分析,指出在隧洞开挖过程中围岩变形的时间效应,为修改设计与指导施工提供理论依据。  相似文献