鳖组织浆对鼠免疫功能的影响   总被引：2，自引：0，他引：2  

钱国英 《水产学报》2000,24(5):476-479

从体内抑制试验和体外培养巨噬细胞的杀瘤试验对甲鱼组织浆的抑瘤作用进行了研究。体内抑瘤试验采用组织浆灌胃方式,计算抑瘤率和生命延长率。体外试验将巨噬细胞与肿瘤细胞共培养,测定ＮＯ浓度与细胞毒效应。培养上清液中一氧化氮（ＮＯ）浓度采用ＮＯ试剂盒检测,细胞毒效应通过ＭＴＴ比色法检测。用黄嘌呤氧化酶法测定用鳖组织浆大鼠脑、肝、血中的超氧化物歧化酶（ＳＯＤ）含量。结果表明,不同来源鳖的效果有区别,野生鳖和仿生养殖鳖２龄与４龄对Ｓ１８０小鼠肿瘤生长有明显的抑制作用（Ｐ〈０．０１）,其抑瘤率分别达７０．６９％和６３．１７％,荷瘤小鼠的生长延长率均显著高于空白对照组（Ｐ〈０．０１）,分别为４０．５９％和３９．９５％;１龄和２龄温室鳖的抑瘤效果也显著（Ｐ〈０．０５）。不同来源和不同年龄的鳖组织抑瘤效果有差异,野生鳖、仿生鳖和温室  相似文献   

FACTORS INFLUENCING SURVIVAL AFTER RADIOTHERAPY OF NASAL TUMORS IN 130 DOGS     

Tracy A.  LaDue  DVM  Richard  Dodge  MS  Rodney L.  Page  DVM  MS  G. Sylvester  Price  DVM  PhD  Marlene L.  Hauck  DVM  PhD  Donald E.  Thrall  DVM  PhD 《Veterinary radiology & ultrasound》1999,40(3):312-317

Improvements in survival of dogs with nasal tumors have been slow to develop throughout the past three decades. Despite multiple studies examining various radiation time-dose schema, the advancement of CT-based computerized treatment planning, and the evaluation of detailed staging systems, the optimal treatment regimen, and most important prognostic factors regarding survival remain unclear. In this study, data from four previous studies were combined with data from 44 additional dogs, and this population of 130 dogs was evaluated for factors which influenced survival. Twenty-one dogs were treated with orthovoltage at the University of Pennsylvania. One hundred nine dogs were treated with cobalt photons at North Carolina State University. Sixty-five of these 109 dogs had been previously described. Of the 44 dogs not previously described, 35 were treated with a shrinking field technique. Survival was determined from the medical record, or from information derived by telephone or mail survey. The univariate Cox regression model was used to examine for relationship between various patient, tumor, and treatment variables and survival. Significant relationships identified in the univariate analysis were further analyzed using the multivariate Cox regression model. Median survival of the 130 dogs was 8.9 months (95% C.I., 8-11 months). In the univariate analysis, the following variables were associated with decreased survival: 1) age >10 years old, 2) regional lymph node metastasis, 3) advanced tumor stage, 4) use of megavoltage radiation, 5) overall total dose >55 Gray, and 6) boost technique performed. In a multivariate analysis of 125 dogs with complete data for age, radiation type, and radiation dose, age (p < .001) and radiation type (p = .02) were identified as joint predictors of survival. After adjusting for age, the staging system lost prognostic significance (p = .06). In a subset of dogs that received cobalt radiation, after adjusting for age, dogs treated with a boost technique had decreased survival (p = .001). In general, local control of canine nasal tumors following aggressive radiation therapy is poor. Early diagnosis and selection of appropriate patients is warranted and palliative types of treatment should be considered in dogs with a poor chance of long term survival.  相似文献   

Expression of podoplanin in various types of feline tumor tissues     

Satoshi KAMOTO  Masahiro SHINADA  Daiki KATO  Masaya TSUBOI  Sho YOSHIMOTO  Ryohei YOSHITAKE  Shotaro ETO  Namiko IKEDA  Yosuke TAKAHASHI  Yuko HASHIMOTO  James CHAMBERS  Kazuyuki UCHIDA  Shinji YAMADA  Mika K. KANEKO  Ryohei NISHIMURA  Yukinari KATO  Takayuki NAKAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(11):1795

Podoplanin is expressed in various human tumors where it promotes tumor progression, epithelial-mesenchymal transition, and distant metastasis. Podoplanin is also expressed in cancer-associated fibroblasts and induces tumor malignancy. The objective of this study was to evaluate podoplanin expression in various types of feline tumor tissues. Immunohistochemical analysis revealed that podoplanin was expressed in cells of 13/15 (87%) squamous cell carcinomas and 5/19 (26%) fibrosarcomas. Moreover, cancer-associated fibroblasts expressed podoplanin in most tumor types, including 18/21 (86%) mammary adenocarcinoma tissues. Our findings demonstrate that various types of feline tumor tissues expressed podoplanin, indicating the importance of the comparative aspects of podoplanin expression, which may be used as a novel research model for podoplanin biology.  相似文献   

Spontaneous hemangiosarcoma in the spleen and liver of a young rat     

Rina Yamane  Makoto Tanaka  Shinya Kaneda 《Journal of toxicologic pathology》2022,35(1):89

Spontaneous hemangiosarcoma in young rats is rare. In this report, we describe a case of a spontaneous hemangiosarcoma in the spleen and liver of young rats. At necropsy, multiple pale red masses were observed in the spleen. Histopathologically, solid growth and haphazardly arranged neoplastic cells were observed, although no characteristic growth pattern was observed. In contrast, irregularly sized small slit-shaped spaces containing erythrocytes were found among the neoplastic cells. Reticular fibers incompletely surrounding the neoplastic cells were observed by silver staining. Immunohistochemistry revealed that the neoplastic cells were positive for vWF and CD34. Electron microscopic examination revealed that the neoplastic cells had erythrocytes in the lumen and Weibel-Palade bodies in the cytoplasm and were arranged along a discontinuous basal lamina. These features indicate that the tumor originated from vascular endothelial cells. Based on these results, the tumor was diagnosed as a hemangiosarcoma in the spleen and liver.  相似文献   

药食用真菌灰树花防治肿瘤相关应用的研究进展     

王亚敏  谢梦洲  张超文  瞿昊宇  向茗  朱建平  肖作为 《农产品加工．学刊》2019,(3):83-86

随着肿瘤发病率的不断上升,寻找针对肿瘤的安全而有效的预防和治疗方法成为肿瘤研究中的迫切关注点,灰树花作为大型的药食用真菌,在近年来的国内外研究中以其显著的抗肿瘤活性引发关注,并引发了全世界对其相关活性和功效机制的深入探索。为了开发一款在防治肿瘤中安全有效的药膳产品,对灰树花肿瘤相关的文献进行了研究,从文献研究中发现,灰树花在抗肿瘤方面具有显著的活性并在肿瘤治疗中有良好的辅助作用,在肿瘤防治有关产品开发方面有巨大的潜力。  相似文献   

薏苡功能性成分研究进展   总被引：2，自引：0，他引：2  

邓素芳  杨有泉  林忠宁  陈敏 《中国农学通报》2017,33(12):123-128

中国薏苡资源非常丰富,具有巨大的医药应用开发价值。为明确薏苡各部位的主要功能性成分,充分挖掘其利用价值,本研究总结了薏苡功能性氨基酸和蛋白质、油脂、维生素和矿物元素、薏苡多糖、多酚等功能性成分的研究进展,结果表明:薏苡非种仁部位同薏苡仁一样富含具有增强免疫、抑制肿瘤生长等多种功能的成分,而且生物量比薏苡仁大,利用前景将更为广阔,并提出利用薏苡非种仁部位代替薏仁开展饲用、药用、食用和日化用的设想,为薏苡功能的深度挖掘和开发利用提供参考。  相似文献   

Protective effect of Yiqi-Wenyang-Huoxue-Huatan formula on HHPH rat brain by suppressing excessive endoplasmic reticulum stress     

XIANG Bing-qian  GAO Hui  JIN Xiao-jie  DAI Yong-yue  QIAN Xiao-ying  CHEN Xi-wen  WANG Wan-tie 《园艺学报》2017,33(10):1759-1767

AIM: To investigate whether Yiqi-Wenyang-Huoxue-Huatan formula (YWHHF) attenuats brain injury induced by hypoxia-hypercapnia pulmonary hypertension (HHPH) in the rats by inhibiting excessive endoplasmic reticulum stress response. METHODS: Healthy SPF male SD rats (n=50) were randomly divided into 5 groups: control group, hypoxia-hypercapnia group, low-dose YWHHF group, middle-dose YWHHF group and high-dose YWHHF group. The rats in control group lived in normal environment, while the rats in other 4 groups were raised for 4 weeks in oxygen tank with low oxygen concentration and high CO₂ concentration. YWHHF was perfused in the rats of low-, middle-and high-dose groups at 0.15, 0.3 and 0.6 g/kg daily, respectively. The rats in hypoxia-hypercapnia group were given isometric distilled water. The surgery was performed on the rats after 4 weeks, and the brain and lung tissues were quickly collected to detect brain water content and observe the morphological changes after mean pulmonary artery pressure recording and heart perfusion. The caspase-3 activity and the apoptotic index of the brain cells were determined. The expression of c-Jun N-terminal kinase (JNK), caspase-12, C/EBP homologous protein (CHOP) and glucose-regulated protein 78 (GRP78) at protein and mRNA levels in brain tissues was detected by Western blot and RT-PCR. RESULTS: Compared with control group, mean pulmonary artery pressure, brain water content, brain apoptotic index, caspase-3 activity, and the protein and mRNA levels of JNK, caspase-12, CHOP and GRP78 in the rest 4 groups were increased, and the brain and lung tissues had obvious damage under light microscope. Compared with hypoxia-hypercapnia group, mean pulmonary artery pressure, brain water content, brain apoptotic index, caspase-3 activity, and the protein and mRNA expression of JNK, caspase-12, CHOP and GRP78 in low-, middle-and high-dose YWHHF groups were decreased, and the pathological damage of the brain and lung tissues was obviously reduced under light microscope. These changes in middle-dose YWHHF group were the most significant. CONCLUSION: YWHHF effectively relieves the brain injury induced by HHPH in rats, which may be associated with inhibiting excessive endoplasmic reticulum stress response.  相似文献   

Brain microvascular basement membrane damage and the expression of uPA and uPA mRNA in brain areas after focal cerebral ischemia/reperfusionin renovascular hypertensive stroke-prone rats     

LI Ling  HUANG Ru-xun  XIAO Xiao-hua  WANG Yi-dong  YIN Mei  CHEN Yan-kui 《园艺学报》2000,16(11):1189-1193

AIM:To investigate the effects of lowdosage of nitric oxide synthase(NOS)inhibitor NG-nitro-L-argi ni ne methyl ester(L-NAME)i n two-week treatment on the hyperdynamic circulatory state i n rats withcirrhosis.METHODS:Cirrhosis model was induced in male SDrats by injection of 60%CCl 4 oily sol utionsubcuta-neously.Cirrhotic rats were treated with L-NAME(0.5 mg·kg^-1·d^-1)by gavage for two weeks.Mean arterial pres-sure(MAP), portal pressure(PP), cardiac output(CO), cardiac index(CI), splanchnic vascular resistance(SVR), splanchnic blood flow(SBF)and serumnitrite levels were determi ned in L-NAME-treated, L-NAME-untreatedcirrhotic rats and controls by usi ng57 Co-labled microsphere technique and a fl uorometric assay, respectively.RESULTS:Untreated cirrhotic rats had significantly lower MAP, SVR and higher PP, CO, CI, SBF and nitrite concentra-tion than those of the controls(all, P<0.01).In treated cirrhotic rats, L-NAME significantly attenuated the in-crease of CO, CI, SBF, nitrite concentration and the decrease of MAP and SVR.Intreated cirrhotic rats, L-NAME induced a marked decrease of nitrite concentrationthan untreated cirrhotic rats[(1.471±0.907)μmol/L vs(4.204±1.253)μmol/L, P<0.01].CONCLUSION:The endogenous NO may play animportant role inthe changes of hemodynamics patterni n cirrhosis, and hyperdynamic circulatory state in rats with cirrhosis can be ameliorated by oral two-week administration of lower dose of L-NAME.  相似文献   

Stability of RNA isolated from post-mortem tissues of Atlantic salmon (<Emphasis Type="Italic">Salmo salar</Emphasis> L.)   总被引：1，自引：0，他引：1  

Seear PJ  Sweeney GE 《Fish physiology and biochemistry》2008,34(1):19-24

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山羊鼻内肿瘤病毒荧光定量PCR检测方法的建立及应用     

肖霜艳  翟少伦  陈小文  谢逸伦  周秀蓉  吕殿红  温肖会  翟颀  贾春玲  魏文康  刘正飞 《中国动物传染病学报》2021,(2):9-14

为了解山羊鼻内肿瘤病毒(ENTV-2)的流行情况,本研究针对ENTV-2的env基因设计了1对特异性引物与探针,通过RT-PCR条件优化,成功建立了ENTV-2荧光RT-qPCR检测方法.该方法特异性高、灵敏性强(5.61 copies/μL)、重复性好(CV为0.09％～1.20％).本研究建立的一步法荧光RT-qP...  相似文献