Anti-Inflammatory Activity and Mechanism of a Lipid Extract from Hard-Shelled Mussel (Mytilus Coruscus) on Chronic Arthritis in Rats     

Guipu Li  Yuanqing Fu  Jusheng Zheng  Duo Li 《Marine drugs》2014,12(2):568-588

The present study was designed to investigate the anti-inflammatory activity and mechanism of a lipid extract from hard-shelled mussel (Mytilus coruscus) on adjuvant-induced (AIA) and collagen-induced arthritis (CIA) in rats. AIA and CIA rats that received hard-shelled mussel lipid extract (HMLE group) at a dose of 100 mg/kg demonstrated significantly lower paw swelling and arthritic index, but higher body weight gain than those which received olive oil (control group). Similar results were found in arthritic rats that received New Zealand green-lipped mussel lipid extract (GMLE) at the same dosage. The levels of leukotriene B₄ (LTB₄), prostaglandin E₂ (PGE₂), thromboxane B₂ (TXB₂) in the serum, and interleukin-1β (IL-1β), IL-6, interferon-γ (INF-γ), tumor necrosis factor-α (TNF-α) in the ankle joint synovial fluids of HMLE group rats were significantly lower than those of control group. However, the levels of IL-4 and IL-10 in HMLE group rats were significantly higher than those in the control group. Decreased mRNA expressions of matrix metalloproteinase 1 (MMP1) and MMP13, but increased tissue inhibitor of metalloproteinase 1 (TIMP1) were observed in the knee joint synovium tissues of HMLE group rats when compared with the control group. No hepatotoxicity was observed in both HMLE and GMLE group rats. The present results indicated that HMLE had a similarly strong anti-inflammatory activity as GMLE. Such a strong efficacy could result from the suppression of inflammatory mediators (LTB₄, PGE₂, TXB₂), pro-inflammatory cytokines (IL-1β, IL-6, INF-γ, TNF-α) and MMPs (MMP1, MMP13), and the promotion of anti-inflammatory cytokines (IL-4, IL-10) and TIMPs (TIMP1) productions.  相似文献   

山羊关节炎-脑炎ELISA方法的建立及其应用     

相文华  吕晓玲 《中国农业科学》1997,30(1):71-76

 应用间接酶联免疫吸附试验（ELISA），检测了山羊关节炎-脑炎病毒（CAEV）试验感染山羊和自然感染山羊的血清抗体，确定了最适宜的反应条件。CAEV感染山羊血清终点滴度结果表明：ELISA比琼脂扩散试验（ID）敏感性高，对自然感染山羊检出率比ID高3%左右。CAE标准阳性血清和自然感染阳性血清预先被特异性抗原中和后的ELISA反应均呈阴性，从而证实了本方法的特异性。因此，本方法适于CAE的流行病学调查和监测工作。  相似文献   

Prostaglandin E₂ receptors,EP2 and EP4, regulate expression of surface molecules and cytokines in B-cells of collagen-induced arthritic mice     

ZHANG Jing-ge  CHEN Hai-ying  QIN Jin  CONG Bin  LI Qiao-xia  JIA Xian-xian  MA Chun-ling  YU Feng 《园艺学报》2011,27(3):545-549

AIM: To observe the immunoregulatory effects of prostaglandin E₂ receptor (EP) subtypes EP2/EP4 on the B-cells of collagen-induced arthritic(CIA)mice. METHODS: DBA/1 mice were immunized with chicken type II collagen emulsified in Freunds complete adjuvant to induce arthritis. B-cells were isolated from the splenocyte suspension by positive selection using anti-CD19 monoclonal antibody immunomagnetic beads. The expression of MHC II, CD 80 and CD86 was examined by flow cytometry. The mRNA levels of EPs, interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), IL-6, IL-4, IL-10 and transforming growth factor-β (TGF-β) were detected by real-time RT-PCR. RESULTS: The rank of the mRNA levels of EPs was EP2>EP1>EP3>EP4 in B-cells and EP2/EP4 mRNA expression was obviously increased in CIA mice. EP2 antagonists inhibited the expression of MHC II, CD80 and CD86. EP4 antagonist had little effect on CD80. EP2/EP4 antagonists inhibited the mRNA expression of IFN-γ, TNF-α, and IL-6 (P<0.05 or P<0.01) and increased the expression of IL-10 (P<0.01 or P<0.05). Furthermore, the antagonists of EP2 and EP4 also increased the mRNA expression of IL-4 and TGF-β (P<0.01), respectively. CONCLUSION: PGE₂ modulate the pathogenesis of CIA via EP2/EP4 by regulating the expression of surface molecules and cytokines in B-cells. EP2/EP4 may be a new therapeutic target for treating rheumatoid arthritis.  相似文献   

MAGNETIC RESONANCE IMAGING IN FOALS WITH INFECTIOUS ARTHRITIS     

LORRIE GASCHEN  ALEXANDRE LEROUX  JESSICA TRICHEL  LAURA RIGGS  HERMAN H. BRAGULLA  NATHALIE RADEMACHER  DANIEL RODRIGUEZ 《Veterinary radiology & ultrasound》2011,52(6):627-633

The magnetic resonance (MR) imaging findings of foals with infectious and noninfectious arthritis are described. Six foals with infectious arthritis and three foals with noninfectious arthritis were grouped based on synovial fluid analysis results and examined with radiography and MR imaging. Four out of six foals with infectious arthritis had osseous lesions in MR images indicative of osteomyelitis and only 4/19 lesions were detected on digital radiographs. The three foals with noninfectious arthritis had no osseous lesions in MR images or radiographically. Of the six joints that had osseous lesions detected with MR imaging, three had at least one lytic lesion detected radiographically. Osseous lesions in the epiphysis, metaphysis, and physis appeared in MR images as T2W, short tau inversion recovery, and proton density hyperintense foci with a hypointense halo. The same lesions appeared hyperintense in the 3D RSSG water excitation pulse sequence but lacked a surrounding hypointense halo. Most joints of foals with infectious arthritis had heterogenous signals within the synovial fluid whereas all of the nonseptic joints had homogenous synovial fluid signals. MR imaging appears to be better than radiography in the detection of osseous lesions in foals diagnosed with infectious arthritis and may be a valuable screening test for the presence of osteomyelitis.  相似文献   

The effect of arthritis in the carpal joint on performance in Norwegian cold-blooded trotters     

N. I. Dolvik  G. Klemetsdal 《Veterinary research communications》1996,20(6):505-512

The purpose of this study was to evaluate the impact of arthritis of the carpal joint on performance of Norwegian cold-blooded trotters. Two performance variables were used in the analyses. The first was the start status, for which horses that had started in one or more races within a certain age received the value 1, and horses that had not raced were correspondingly assigned the value 0. The second variable was the accumulated, transformed and standardized earnings (ATSE), which is the power transformation of earnings (earnings^0.2, with unraced horses assigned a value of zero) expressed as a standardized normal deviate by birth year. With the exception of the first year of racing, the number of horses that had raced was larger in the group of unaffected horses than in the groups with arthritis (carpitis or bilateral carpitis), although the difference between the groups was not significant for any of the age classes. A similar picture was observed for ATSE and, in general, the diseased horses earned less money. None of these differences was significant at the 5% level. However, the statistical power was less than 0.3, which means that the probability of detecting a true difference was less than 30%. The data were deemed inadequate to show a significant effect of arthritis on racing performance. This may only be achieved through investigations in which more of the error variance can be statistically modelled, and in which arthritis can be observed as an incidence rather than as a prevalence.Abbreviations ATSE accumulated, transformed and standardized earnings  相似文献   

DIAGNOSTIC ULTRASOUND IMAGING OF SOFT TISSUES IN THE BOVINE DISTAL LIMB   总被引：4，自引：0，他引：4  

Johann  Kofler  Dr. Med. Vet  Hannes K.  Edinger  Dr. Med. Vet. 《Veterinary radiology & ultrasound》1995,36(3):246-252

In this study sonographic images of healthy flexor tendons, digital flexor tendon sheaths, metacarpophalangeal and metatarsophalangeal joints, and proximal and distal interphalangeal joints of the bovine limb were made. Then, synovial cavities in cadavers were filled with the necessary amount of fluid to make the lumina visible sonographically. After injecting 30–40 ml of saline solution into the digital flexor tendon sheath and into the metacarpophalangeal and metatarsophalangeal joints, or 8 ml in the proximal interphalangeal joint and 10 ml in the distal interphalangeal joint, the pouches of these synovial cavities were clearly demarcated. Afterwards, the sonographic image of synovial cavities distended by various inflammatory content were described. When the sonographic findings were compared to the findings in clinical patients, centesis and intraoperative procedures, there appeared to be a relationship as to the extent and location of the disorders as well as to the nature of the synovial effusion. Finally, we identified which planes on diseased bovine distal limbs were appropriate for obtaining optimal sonographic images.  相似文献   

Preliminary observations on the effects of Meloxicam in a new model for acute intra-articular inflammation in dogs   总被引：1，自引：0，他引：1  

H. van Bree  C. Justus  J. F. Quirke 《Veterinary research communications》1994,18(3):217-224

The effects of intra-articular injection, on two occasions, 3 weeks apart, of the contrast agent Urografin on the cytological and biochemical characteristics of synovial fluid (SF) were examined in two studies in dogs. The first study provided baseline data in two non-medicated dogs. The second study used a cross-over design whereby 4 dogs received a 7-day oral treatment with either a placebo or meloxicam (0.2 mg/kg body weight daily) with a washout period of 3 weeks, in order to determine the effect of this new non-steroidal anti-inflammatory drug (NSAID) on the response to Urografin injection. SF samples were collected under general anaesthesia prior to and at 24 and 72 h after each Urografin injection. The volume, relative viscosity, white blood cell count and concentrations of protein, lactate dehydrogenase (LDH) and hyaluronic acid of these samples were determined.The results from both studies indicate that intra-articular injection of Urografin provoked a mild local transient inflammatory response, the most dramatic evidence of which was an increase in the white blood cell count in the SF after 24 h. In the second study, comparison of the synovial fluid measurements of the placebo-treated dogs at 24 h after Urografin injection with those prior to injection revealed significant increases in SF volume, white blood cell count, protein concentration and LDH activity and a significant reduction in relative viscosity. At 72 h after injection, only the white blood cell count and relative viscosity were significantly different from the pre-injection values. All of these measurements were, however, associated with high coefficients of variation, which must be taken into account in assessing the usefulness of the model for drug-testing purposes. Nevertheless, the administration of meloxicam significantly reduced the SF volume and white blood cell count at 24 h relative to the effects of concurrent placebo treatment. The general health status of the animals was not disturbed at any time as assessed by clinical and haematological observations. No adverse reactions were observed.Abbreviations LDH lactate dehydrogenase - NSAID non-steroidal anti-inflammatory drug - SF synovial fluid - WBC white blood cell count  相似文献   

脊髓CGRP在大鼠实验性关节炎形成和发展中的作用     

李文武  朱丽霞  吉长福  张金玲 《中国兽医学报》1997,(2)

采用鞘内注射降钙素基因相关肽（ＣＧＲＰ）受体拮抗剂人ＣＧＲＰ片段８－３７（ｈＣＧＲＰ８－３７），探讨了佐剂性关节炎大鼠初级感觉系统中ＣＧＲＰ增多的意义。结果：佐剂前注射ｈＣＧＲＰ８－３７的动物，在注射佐剂后１～２０ｄ，患肢及健肢皮温均高于对照组（Ｐ＜０．０５）；患肢足容积明显大于对照组（Ｐ＜０．０５），并且在形成变态反应性佐剂性关节炎时（佐剂后１３～２０ｄ），健肢足容积也明显增加（Ｐ＜０．０５）；但双后肢的痛阈变化与对照组相比没有明显差异（Ｐ＞０．０５）。上述结果表明，佐剂前鞘内注射ｈＣＧＲＰ８－３７，使急性关节炎和多发性关节炎均有所加重。佐剂后１２ｄ注射ｈＣ－ＧＲＰ８－３７的动物，双后肢的肿胀积分均明显高于对照组（佐剂后１８～５０ｄ，Ｐ＜０．０５或０．０１），痛级别也明显高于对照组（佐剂后１４～５０ｄ，Ｐ＜０．０１），但痛阈的变化不明显（Ｐ＞０．０５）。因此佐剂后１２ｄ鞘内注射ＣＧＲＰ的拮抗剂也可加重关节炎的炎症反应。这进一步证明了脊髓ＣＧＲＰ可能具有抗炎和抑制免疫反应的作用。  相似文献   

SEPTIC COXOFEMORAL ARTHRITIS AND OSTEOMYELITIS IN A DOG     

Lyndell  Levitt  BS  DVM  J. David  Fowler  BS  DVM  MVSc 《Veterinary radiology & ultrasound》1988,29(3):129-132

An 8-year-old male Labrador retriever presented with a rear limb lameness cased by an osteolytic lesion of the left acetabulum and femoral head and neck. Initial bone biopsy study was nondiagnostic. Histopathologic study and culture of the subsequently excised femoral head and neck revealed a Staphyloccocus intermedial arthritis and osteomyelitis. Neoplash was ruled out on the basis of histopathologic analysis.  相似文献   

A STORAGE-LIKE DISEASE WITH SKELETAL INVOLVEMENT IN A DOG     

Cecil E. Doige  Charles S. Farrow  Billy R. Gee 《Veterinary radiology & ultrasound》1980,21(3):98-103

A storage-like disease in a 14-month-old Rhodesian Ridgeback dog is described. Clinically the dog was lame on all legs, and appendicular joint capsules were distended. Radiographically and at necropsy, there was evidence of osteoarthritis, increased volume of synovial fluid, and marked hypertrophy of the synovial membranes. Large mononclear cells with vacuolated cytoplasm were evident in synovial membranes. Large mononuclear cells with vacuolated cytoplasm were evident in synovial membranes. Large mononuclear cells with vacuolated cytoplasm were evident in synovial membranes, bone marrow, liver, lymph nodes, and endocrine glands. Vacuolated cells in lymph nodes stained positively for fat. Transmission electron microscopy revealed empty membrane-bound cytoplasmic vacuoles. The similarity of this disease to storage diseases in man where arthropathy is a feature is discussed.  相似文献