林可霉素水溶性粉剂对由魏氏梭菌引起的鸡坏死性肠炎的药效研究     

沈建忠  肖希龙 《畜牧兽医学报》1997,28(5):464-468

本文对新兽药制剂林可霉素水溶性剂，用人工感染发病的方法进行了药效学研究。  相似文献   

鸭肠炎病毒UL41基因缺失株的构建及其体外增殖能力分析     

杨伏春  刘芮  李晓涵  高立  刘长军  祁小乐  崔红玉  王笑梅  高玉龙  李凯 《畜牧兽医学报》2022,53(8):2689-2696

旨在构建鸭肠炎病毒（DEV）UL41基因缺失毒株,并对其生物学特性进行分析,本研究以克隆有DEV UL41基因的重组黏粒D1为骨架,利用Red/ET重组技术构建缺失UL41基因的重组黏粒D1 dUL41;将UL41基因缺失重组黏粒D1 dUL41与其他4个克隆有DEV基因组片段的亲本黏粒共转染鸭胚成纤维细胞（DEF）,拯救获得UL41基因缺失毒株rDEV-SD19/dUL41。将该基因缺失病毒感染DEF后,提取病毒基因组进行PCR鉴定及测序,并利用间接免疫荧光试验检测该病毒感染细胞中UL41基因表达情况;绘制拯救的基因缺失病毒的生长曲线,分析其体外复制特性。PCR及测序结果显示,本研究成功构建了缺失UL41基因的重组黏粒D1 dUL41。将该基因缺失黏粒与其他含有DEV基因组的亲本黏粒共转染DEF后能够产生典型的蚀斑病变。PCR及测序结果显示,UL41基因成功从DEV基因组中缺失;间接免疫荧光试验发现,基因缺失病毒rDEV-SD19/dUL41感染DEF后,未见UL41蛋白表达。综上表明,本研究成功构建了DEV UL41基因缺失病毒。体外生长曲线显示,rDEV-SD19/dUL41在DEF中的复制能力明显低于亲本病毒,提示UL41蛋白在DEV复制中发挥重要作用。UL41基因缺失DEV的构建为进一步研究UL41基因在DEV感染和致病中的作用机制奠定了基础。  相似文献   

A framework for assessing the efficacy of antimicrobials in the control of necrotic enteritis in broiler chickens     

《The Journal of Applied Poultry Research》2015,24(2):246-256

This review presents a framework for assessing the efficacy of antimicrobials used to control necrotic enteritis (NE) caused byClostridium perfringens (CP) in the context of susceptibility testing and clinical efficacy, and their potential interactions with the intestinal microbiota of poultry. Practitioners have traditionally based their choice of antimicrobial agent on antimicrobial susceptibility testing, but there appears to be a lack of correlation with clinical efficacy for in-feed antimicrobials (particularly bacitracin and virginiamycin). Resistance patterns of CP and antimicrobials have been monitored using epidemiological cutoffs for minimal inhibitory concentration (MIC), which are not intended to guide therapy. Also, most data have been determined using CP isolates from healthy birds (i.e., potentially from commensal strains not known to be clinically relevant). It is believed that NE is caused by specific virulent CP strains (and potentially other bacteria) that proliferate and displace these commensals. The presence of resistant commensals is not necessarily detrimental (and may even be beneficial) if they inhibit the single CP strain dominance effect observed in acute NE. The choice of antimicrobial therapy in a clinical setting should thus be based on a variety of factors, including an accurate diagnosis, results of efficacy studies, prior experience at the premises in question, and interpretation of MIC data, recognizing that it is not necessarily well correlated with clinical efficacy.  相似文献   

罗非鱼鱼种肠炎病的病理组织学及病原体的研究     

张晓君  田在滋 《河北渔业》1996,(5):5-7

本文从病理组织学的角度,研究了罗非鱼鱼种肠炎病的发生与发展,为其诊断与防治奠定了理论基础。结果表明,该病病原体为产气单胞菌。  相似文献   

A novel 'skinny pot-belly' disease in Asian seabass fry, Lates calcarifer (Bloch)     

Gibson-Kueh S  Crumlish M  Ferguson HW 《Journal of fish diseases》2004,27(12):731-735

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一种应用LORF11同源子鉴别诊断鸭瘟病毒的PCR方法     

许梦微  张传健  周希  王志胜  侯继波  王继春 《江苏农业学报》2014,(5)

为建立一种鉴别诊断鸭瘟病毒的PCR方法,根据已发表的8株鸭瘟病毒株的长区开放阅读框(LORF11)等位基因序列,在其开放阅读框等位基因的上游5 bp和下游101 bp的位置设计1对特异性引物。应用这对引物对鸭瘟病毒LH2011株、v2085株、VAC株、Clone-03株DNA进行PCR扩增,分别获得了4 448 bp、3 277bp、934 bp和2 518 bp的特异性条带。此PCR方法特异性强,敏感性高,可检测到10TCID50或0.1LD50的鸭瘟病毒。在感染鸭瘟病毒的组织(肝脏)中均能检测到鸭瘟病毒DNA。对疑似鸭瘟的临床病料的检测结果也证明,建立的PCR方法不仅能够鉴别样品是否感染了鸭瘟病毒,而且能鉴别感染的鸭瘟病毒的来源。  相似文献   

Full-fat soybean meal in diets for Atlantic halibut: growth, metabolism and intestinal histology   总被引：1，自引：0，他引：1  

B. GRISDALE-HELLAND  S.J. HELLAND  G. BAEVERFJORD  & G.M. BERGE 《Aquaculture Nutrition》2002,8(4):265-270

Isonitrogenous and isocaloric diets containing 0, 18 or 36% toasted full-fat soybean meal (FFSM) were fed to Atlantic halibut. The diets were fed to five tanks of fish each for 34 days (period 1). Four tanks from each treatment were then retained in the growth experiment for a further 32 days (period 2), while the groups of fish from one tank from each of the 0 and 36% FFSM groups were split and transferred to two metabolism tanks each. The initial weight of the fish in the growth trial was 169 ± 1 g (mean ± SEM, n =12; weight range 89–253 g) and the final weight was 317 ± 5 g. There was no significant effect of dietary treatment on specific growth rate (range, 0.8–1.1% day⁻¹), feed consumption (0.5–0.7% body weight day⁻¹), feed efficiency (1.3–1.6 g wet gain g dry feed⁻¹), protein retention (48–55%) or energy retention (49–57%). The fat, protein and energy concentrations in the fish increased during the trial and were not affected by the diet. The hepatosomatic index in fish fed with 36% FFSM diet was significantly lower (1.7%) than in the other groups (2.2%) ( P  < 0.05). No differences in intestinal morphology were observed between dietary treatments and no pathological reactions were identified in any of the samples. In the metabolism trial, there were no significant differences in oxygen consumption or ammonia excretion between fish fed with 0 and 36% FFSM diets. In conclusion, up to 36% FFSM may be added to diets for Atlantic halibut without negative effects on growth, feed efficiency or intestinal morphology.  相似文献   

多种接种途径对草鱼细菌性烂鳃和肠炎病的免疫预防效果   总被引：1，自引：0，他引：1  

陈昌福 《华中农业大学学报》1997,16(5)

将柱状嗜纤维菌（Ｃｙｔｏｐｈａｇａｃｏｌｕｍｎａｒｉｓ）和点状气单胞菌（Ａｅｒｏｍｏｎａｓｐｕｎｃｔａｔａ）分别制成酚灭活菌苗（ＰＫＣ）和提取其菌体脂多糖（ＬＰＳ），采用浸泡加注射和口服加浸泡的多种接种途径，对草鱼免疫接种４周后，测定受免鱼血清中凝集抗体效价。无论是浸泡加注射还是口服加浸泡的免疫应答均较对照鱼有明显上升，对活菌攻毒的免疫保护率也达到了５０％～８８．９％。证明了多种接种途径免疫预防草鱼的细菌性烂鳃和肠炎病的效果是明显的。  相似文献   

貂,貉,犬病毒性肠炎快速诊断试剂的研究   总被引：1，自引：0，他引：1  

刘润珍  李世雄 《野生动物》1996,(6):28-30

用金黄葡萄球菌Ａ蛋白（ＳＰＡ）协同凝集方法（ＣｏＡ）对貂、貉、犬病毒性肠炎快速诊断的试剂进行了制备，确定了制备。艺及该制剂的标准操作程序。本制剂特异性强，不与健康动物的组织及其他几种病毒病（犬瘟热、犬传染性肝炎、狂犬病等）发生交叉凝集反应，灵敏度高，通过该制剂ＣｏＡ法与ＨＡ－ＨＩ法比较结果比现行的貂、貉、犬病毒性肠炎ＨＡ－ＨＩ诊断方法检出率高出３０％，同时具有微量、操作简便、易掌握、快速等特点，可在３～５ｍｉｎ获得诊断结果。  相似文献   

牛出血性肠炎A型产气荚膜梭菌的分离鉴定     

孙连童  吴建勇  李建军  杨魁  段新华  王治才 《中国动物传染病学报》2012,20(5):27-31

从新疆维吾尔自治区某牛场采集的3份疑似出血性肠炎病料中分离到8株产气荚膜梭菌,用PCR扩增保守基因16SrRNA,并进行序列测定和同源性分析,再通过多重PCR方法扩增型特异性基因进行分离菌株的分型鉴定。结果显示,所分离的8株产气荚膜梭菌之间16S rRNA基因同源型为100%,与GenBank参比序列同源性在99.8%以上,确定为产气荚膜梭菌。遗传进化分析表明,本次分离的8株产气荚膜梭菌之间拥有共同起源,但与所用的参考菌株分属不同来源。多重PCR扩增结果显示,8株菌株均为产气荚膜梭菌A型。  相似文献