鸭肠炎病毒PCR产物的克隆及鉴定   总被引：1，自引：0，他引：1  

谢芝勋  谢志勤  刘加波  邓显文  庞耀珊  廖敏 《中国兽医学报》2001,21(4):338-340

根据已发达的鸭肠炎病毒（DEV）基因的核苷酸序列，设计并合成了1对引物，以鸭肠炎病毒DNA为模板扩增出602bp的基因片段，将该基因片段通过粘端连接克隆到质粒pBluescipt ⅡKS^ 中，重组质粒转化大肠杆菌DH5α感受态细胸质，在LB平板上筛选重组菌，重组子经聚合酶链反应（PCR）和HindⅢ与PstⅠ双酶切鉴定，该基因片段已成功的克隆到质粒载体上。  相似文献   

Evidence of Rotavirus Associated with Neonatal Lamb Diarrhoea in India     

Wani SA  Bhat MA  Nawchoo R  Munshi ZH  Bach AS 《Tropical animal health and production》2004,36(1):27-32

Tropical Animal Health and Production -  相似文献   

Antibiotic-responsive histiocytic ulcerative colitis in 9 dogs   总被引：6，自引：0，他引：6  

Hostutler RA  Luria BJ  Johnson SE  Weisbrode SE  Sherding RG  Jaeger JQ  Guilford WG 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(4):499-504

Canine histiocytic ulcerative colitis (HUC) is characterized by colonic inflammation with predominantly periodic acid-Schiff (PAS)-positive macrophages. The inflammation results in colonic thickening, ulcerations, and distortion of normal glandular architecture. Resultant clinical signs consist of chronic large bowel diarrhea, tenesmus, and marked weight loss, and the disease frequently results in euthanasia. Conventional therapy consists of some combination of prednisone, azathioprine, sulfasalazine, and metronidazole. Nine dogs (8 Boxers and 1 English Bulldog) with histologic confirmation of HUC were treated with antibiotic therapy (either with enrofloxacin alone or in combination with metronidazole and amoxicillin). Clinical signs, physical examination findings, laboratory abnormalities, and the histologic severity of the disease were evaluated. Four of the 9 dogs had been treated previously with conventional therapy and had failed to respond favorably; then, these dogs were placed on antibiotic therapy (enrofloxacin, n = 1; enrofloxacin, metronidazole, and amoxicillin, n = 3) and had resolution of clinical signs within 3-12 days. Five dogs were treated solely with antibiotic therapy (enrofloxacin, n = 1; enrofloxacin and metronidazole, n = 1; enrofloxacin, metronidazole, and amoxicillin, n = 3), and clinical signs resolved in 2-7 days. Repeated biopsy specimens were obtained from 5 dogs after treatment, and all showed marked histologic improvement. The increase in body weight after treatment was statistically significant (P = .01). Three dogs currently are not on any treatment and have had resolution of clinical signs for up to 14 months. These observations suggest that an infectious agent responsive to antibiotics plays an integral role in the clinical manifestation of canine HUC, and they support the use of antibiotics in its treatment.  相似文献   

Preliminary testing in turkeys of the safety and efficacy of a putative haemorrhagic enteritis virus vaccine     

Phuong Hoang  Priscilla F. Gerber  Paul Reynolds  Mary McMillan  Peter Gray  Stephen W. Walkden‐Brown 《Australian veterinary journal》2019,97(9):323-332

Haemorrhagic enteritis virus (HEV) causes clinical haemorrhagic enteritis in young poults and/or subclinical immunosuppression which is often associated with colibacillosis. This disease is controlled with live vaccines worldwide, however, importation of HEV vaccines or cells that support HEV propagation are not permitted in Australia. A major experiment in isolators was conducted to test the safety and efficacy of a putative HEV vaccine. The study had a factorial design with four factors namely vaccination age (28 and 42 days of age), vaccine dose (0, 10⁵, 10⁶, 10⁷ genomic copies of HEV vaccine), challenge with HEV (yes, no) and vaccination‐challenge interval (7, 21 or 42 days). A total of 315 poults were used providing 6‐8 birds per treatment combination. Turkey growth rate, mortality, pathological findings, anti‐HEV antibodies and viral load were examined. Vaccination lead to significant increases in anti HEV antibody over the following 2‐4 weeks. Overall, vaccination with 10⁶ and 10⁷ was protective against increase in relative splenic weight and splenic viral load in challenged birds. Clinical haemorrhagic enteritis was not induced by any treatment but there was an increased incidence of airsacculitis in groups receiving either HEV vaccine or challenge virus compared to the negative control birds (25.8‐29.3% vs. 9.4%, P < 0.05). Growth rate, mortality and relative bursal weight were unaffected by vaccination. This laboratory level study indicates that the putative vaccine is safe and likely to be efficacious, but may cause elevated levels of airsacculitis. These findings require confirmation in larger scale field trials.  相似文献   

Characterisation of the inflammatory reaction in equine idiopathic focal eosinophilic enteritis and diffuse eosinophilic enteritis     

Mäkinen PE  Archer DC  Baptiste KE  Malbon A  Proudman CJ  Kipar A 《Equine veterinary journal》2008,40(4):386-392

REASONS FOR PERFORMING STUDY: Idiopathic focal eosinophilic enteritis (IFEE) and diffuse eosinophilic enteritis (DEE) are primary eosinophilic intestinal conditions without a known cause that are associated with an increasing number of surgical colic cases. Histology may be helpful in defining disease aetiology and pathogenesis. OBJECTIVES: To characterise further the inflammatory infiltrate in equine IFEE and to compare the condition with DEE. METHODS: Twenty-three IFEE cases and 5 DEE cases were examined by light microscopy including immunohistology to identify infiltrating leucocytes. Inflammatory infiltrates in mucosa and submucosa were characterised in IFEE lesions (Group 1), the intestine distant from the lesions in IFEE (Group 2) and DEE (Group 3). RESULTS AND CONCLUSIONS: IFEE lesions represented an accumulation of leucocytes in submucosa and muscularis, with dominance of eosinophils and macrophages and smaller numbers of lymphocytes, plasma cells and neutrophils. T cells represented the dominant lymphocytes. The mucosa overlying the lesion and both mucosa and submucosa in IFEE nonlesion sites and in DEE exhibited a similar composition, with different prevalence of various cell types. Macrophages were significantly more prevalent in the mucosal and submucosal infiltrates in IFEE nonlesion sites than in DEE, and lymphocytes significantly more prevalent in the mucosa in DEE than in IFEE nonlesion sites. The findings confirm IFEE as a primary eosinophilic intestinal disorder and indicate that IFEE represents a focally exacerbated inflammatory reaction in horses with DEE, possibly due to functional changes in the macrophage and T cell components, with subsequent excessive recruitment of both eosinophils and macrophages.  相似文献   

鸡坏死性肠炎生物性防控研究进展     

阚刘刚  刘艳  吴媛媛  范文晓  王忠 《畜牧兽医学报》2019,(6):1123-1134

鸡坏死性肠炎(necrotic enteritis,NE)是现代化肉鸡集约养殖生产中最为突出的肠道疾病之一,给家禽产业带来了巨大的经济损失。随着世界范围内的立法限制、禁用抗生素生长促进剂,鸡坏死性肠炎的发病率在全球范围内呈上升趋势,这对家禽的生产和健康产生严重威胁。因此,迫切需要新的技术和新的产品代替抗生素来预防和控制鸡坏死性肠炎。作者着眼于NE的危害和疫苗、噬菌体、卵黄抗体、植物提取物、酸化剂、益生菌、低聚糖与微生物多糖等生物性防控进展,综述了目前有效预防和控制鸡坏死性肠炎的生物性措施的最新研究情况,既为鸡坏死性肠炎生物性防控提供技术参考,也为其深入研究提供了理论思考。  相似文献   

First detection of bovine noroviruses and detection of bovine coronavirus in Australian dairy cattle          下载免费PDF全文

SJ Symes  JL Allen  PD Mansell  KL Woodward  KE Bailey  JR Gilkerson  GF Browning 《Australian veterinary journal》2018,96(6):203-208

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Campylobacteriosis in dogs and cats: a review     

E Acke 《New Zealand veterinary journal》2018,66(5):221-228

Campylobacter species are commonly isolated from faecal samples collected from dogs and cats, with the most prevalent species being C. upsaliensis, C. helveticus, and C. jejuni. Although the majority of dogs and cats are subclinically infected, some will develop mild to moderate enteritis. Immature animals, animals from intensive housing backgrounds, and animals with concurrent disease are especially predisposed to infection and the development of clinical signs. Bacterial culture methods applied in diagnostic laboratories remain biased to C. jejuni and C. coli detection, but molecular methods to diagnose Campylobacter spp. infections in dogs and cats have become widely available and can aid rapid and accurate diagnosis. Multilocus sequence typing has also been developed for subtyping different strains and has been used in epidemiological investigations. In the majority of cases, clinical signs are self-limiting and antimicrobial treatment is not warranted. Campylobacter spp. isolated from dogs and cats have shown resistance to commonly used antimicrobials, so antimicrobial therapy should only be administered where this is justified. Contact with dogs and cats is a recognised risk factor for human campylobacteriosis, thus people living or working in close contact with cats and dogs should be made aware of the zoonotic organisms these animals can shed.  相似文献   

Effects of the butyric acid‐producing strain Clostridium butyricum MIYAIRI 588 on broiler and piglet zootechnical performance and prevention of necrotic enteritis          下载免费PDF全文

Motomichi Takahashi  Elinor McCartney  Anne Knox  Maria Francesch  Kentaro Oka  Kaoruko Wada  Marie Ideno  Koji Uno  Krzysztof Kozłowski  Jan Jankowski  Marta I. Gracia  Joaquin Morales  Spyridon K. Kritas  Enric Esteve‐Garcia  Shigeru Kamiya 《Animal Science Journal》2018,89(6):895-905

The objective of this study was to assess the effects of a probiotic strain Clostridium butyricumMIYAIRI 588 (CBM588) on broiler and weaned piglet health and zootechnical performance. Five field studies were carried out in broilers and five in weaned piglets under European feed additive guidelines. Each study followed a randomized blocked design with two treatments: Control (basal diet) and CBM588 supplemented groups. The zootechnical performance parameters selected were body weight, daily gain, feed intake and feed efficiency (feed:gain). Broilers fed diets with CBM588 gained significantly more weight (+2%, p < .001) and exhibited significantly better feed efficiency (?1.6%, p < .001) in comparison with Controls. Similarly, analysis of pooled data of weaned piglet trials showed that CBM588‐fed piglets were significantly heavier than Controls (+2.6%, p = .014), exhibited significantly higher mean daily gain (+4.7%; p = .004), and significantly improved feed efficiency (?4.2%, p = .001). In addition to the zootechnical efficacy studies, the preventive effect of CBM588 on necrotic enteritis (NE) was assessed in a natural challenge model in broilers where CBM588 reduced the incidence and severity of NE lesions. These data indicate the potential of CBM588 to improve broiler and weaned piglet zootechnical performance, and to make a positive contribution to animal health.  相似文献   

Ultrasonographic intestinal muscularis thickening in dogs with histologically confirmed inflammatory bowel disease: 13 cases (2010–2021)     

Alexandra G. Collins-Webb DVM  Deborah LA. Chong BVSc  Stacy D. Cooley DVM 《Veterinary radiology & ultrasound》2023,64(2):345-350

Ultrasonographic intestinal muscularis thickening has not been described as an imaging feature of canine inflammatory bowel disease. In this retrospective case series, patients were identified by searching sonographic reports for “muscularis” and/or “muscular layer.” Patients were included if small intestinal muscularis thickening was reported, and sonographic images and histopathological samples of the small intestine were available for review. Cases with small intestines nodules, masses, or complete loss of wall layering were excluded. Sonographic images were retrospectively evaluated for jejunal muscularis layer thickness, and ratios of intestinal layer measurements were performed. Histological samples were retrospectively reviewed. Thirteen dogs met inclusion criteria: all dogs had sonographic intestinal muscularis thickening relative to the submucosa (>1.0, range of 1.3–2.5), and most dogs had muscular layer thickness above normal published ranges (11/13; all 13/13 above the weight-specific mean). More than half of the patients had overall normal wall thickness (11/13) and several had normal mucosal echogenicity (6/13). Therefore, in some dogs, the only sonographic abnormality in the small intestine was muscularis thickening. No dogs had lymphadenomegaly. Endoscopic partial-thickness (n = 11, duodenum and/or ileum) or surgical full-thickness (n = 2) samples confirmed inflammatory bowel disease. Direct comparison between jejunum sonographic characteristics and histology features was limited due to both partial thickness biopsies and lack of direct comparison between anatomical locations of ultrasonographic assessment and biopsy site. However, no cases that met the inclusion criteria had normal small intestinal histology. Comparable to cats, dogs with ultrasonographic intestinal muscularis thickening may have inflammatory bowel disease, and further workup for enteropathy is indicated.  相似文献