cDNA cloning and complete primary structures of myosin heavy chains from brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso fast skeletal muscles     

Mohammed Anwar Hossain  Daisuke Ikeda  Akira Nomura  Hideto Fukushima  Shugo Watabe 《Fisheries Science》2008,74(4):921-934

ABSTRACT:   The complete cDNA sequences encoding predominant types of myosin heavy chain (MYH) in the fast skeletal muscle were determined for brushtooth lizardfish Saurida undosquamis and wanieso lizardfish S. wanieso , which are used as materials for preparing high-quality surimi-based products. The cDNA consisted of 5973 and 5987 bp, respectively, and both encompassed an open reading frame encoding a polypeptide of 1936 amino acid residues. Brushtooth and wanieso lizardfish MYH showed the amino acid sequence identity of 92–93% to white croaker MYH, which was higher than that of 90% to walleye pollack MYH. The putative binding sites for ATP, actin, and regulatory and essential light chains in the subfragment-1 region of brushtooth lizardfish MYH exhibited a high identity with white croaker counterparts as well as the sequences of subfragment-2 and light meromyosin. In contrast, phylogenetic tree, constructed by the neighbor-joining method based on mitochondrial 16S rRNA gene, revealed that the two lizardfish species formed a cluster with walleye pollack, which was paraphyletic with white croaker. Therefore, a good reputation for lizardfish and white croaker to have a high thermal-gel forming ability seemed to be reflected by MYH rather than biological similarity as revealed by the mitochondrial 16S rRNA gene.  相似文献   

基于阵列芯片现场高灵敏同步快速检测粮油中氨基甲酸酯类农药残留混合污染     

周丹  闵亚杰  戚欣  宁佳囡  吴文琴  张兆威  李培武 《中国油料作物学报》2021,42(3):341

The quality and safety of agricultural products is the technique focus of the strategy of rural vitaliza⁃tion. Carbamate pesticide residues are seriously threatening the safety of agricultural product and human life and health. Nowadays, the common detection methods are instrument-based for carbamate pesticide residue, which dis⁃ satisfy the request of an on-site rapid, sensitive and simultaneous detection for multiple carbamate pesticide resi⁃ dues. Based on the home-made monoclonal antibodies against carbofuran and carbaryl respectivity, a microarray for rapid and simultaneous detection of multiple carbamate pesticide residues was developed within 30 min. Under the optimized conditions, the limits of detection（LODs）were found to be 0.11 and 0.09 ng/g, while the linear ranges were 0.33-3000 and 0.18-1500 ng/g for carbofuran and carbaryl respectively. By using spiked peanut samples, it recorded recoveries of 88.4%-98.3%, 90.1%-118.8% for carbofuran and carbaryl respectively. The RSD of intraarray were found at 9.0%, 3.5%, while the RSD of inter-array were recorded at 12.3%, 16.3% respectively. The de⁃ tection results of the microarray were highly consistent with that of HPLC-MS/MS. This proposed microarray provid⁃ ed an on-site high-sensitivity, simultaneous and rapid detection of carbofuran and carbaryl, and could be widely used in the fields of agricultural product quality safety, food safety and environmental monitoring.  相似文献   

合方鲫及其亲本肌肉营养成分分析   总被引：1，自引：0，他引：1  

刘庆峰  王静  肖军  陈璇  齐艳华  李武辉  陶敏  张纯  覃钦博  罗凯坤  刘少军 《水产学报》2017,41(7):1133-1139

采用生化分析手段对合方鲫及其亲本的肌肉营养成分进行比较研究。结果显示,合方鲫肌肉中水分含量为71.00%,显著低于其亲本的水分含量(日本白鲫:75.60%,红鲫:75.50%),说明合方鲫具有低水分的特征。合方鲫的蛋白质含量为17.70%,高于红鲫的蛋白质含量(17.00%),且显著高于日本白鲫的蛋白质含量(14.80%)。在15种检测的氨基酸含量中,合方鲫的氨基酸总量(15.87%)、必需氨基酸总含量(6.55%)均高于红鲫的氨基酸总量(15.52%)和必需氨基酸总含量(6.46%),且显著高于日本白鲫的氨基酸总量(13.13%)和必需氨基酸总含量(5.27%)。值得一提的是合方鲫的呈味氨基酸总含量高达6.26%,高于红鲫的呈味氨基酸总含量(6.07%),且显著高于日本白鲫的呈味氨基酸总含量(5.29%)。研究表明,合方鲫是一种营养价值高、肉味鲜美的鱼类,为其生产上的应用提供了理论支撑。  相似文献   

杜仲对凡纳滨对虾生长、血清非特异性免疫和肌肉成分的影响     

刘波  冷向军  李小勤  李乃顺  柴仙琦 《中国水产科学》2013,20(4):869-875

在基础饲料中分别添加0.0%(对照组)、0.5%、1.0%、2.0%和3.0%的杜仲(Eucommia ulmoides), 饲喂体质量为(7.5±0.2) g的凡纳滨对虾(Litopenaeus vannamei), 实验共分5个处理组, 每处理组4个重复, 每重复40尾虾。经过42 d养殖, 各处理组均有较高的存活率, 且无显著差异(P>0.05); 2.0%杜仲组的虾体增重率最高(136.1%), 饲料系数最低(1.33), 较对照组提高增重率9.8%(P<0.05), 降低饲料系数0.13(P<0.05); 饲料中添加0.5%、1.0%杜仲, 显著提高了对虾血清LSZ、PO活性, 添加1.0%杜仲, 显著降低了血清MDA含量, 提高了肝胰腺蛋白酶活性(P<0.05); 攻毒实验结果表明, 以溶藻弧菌(Vibrio alginolyticus)肌肉注射虾体后96 h, 0.5%、1.0%、2.0%杜仲组的虾体死亡率均较对照组显著降低(P<0.05); 在肌肉成分方面, 添加2.0%、3.0%杜仲显著提高了肌肉胶原蛋白含量, 各处理在肌肉水分、灰分、粗蛋白、粗脂肪含量方面没有显著差异。上述研究表明, 在凡纳滨对虾饲料中添加杜仲2.0%, 可显著改善生产性能, 提高肌肉胶原蛋白含量; 在饲料中添加杜仲0.5%～1.0%, 可提高凡纳滨对虾非特异性免疫能力。本研究旨在考察杜仲对凡纳滨对虾生长、血清非特异性免疫和肌肉成分的影响, 为杜仲在对虾饲料中的合理应用提供科学依据。

  相似文献   

华鲮肌肉营养成分与品质的评价   总被引：12，自引：2，他引：12  

周兴华  郑曙明  吴青  向枭  陈建 《淡水渔业》2007,37(1):62-65

利用常规肌肉营养测试方法测定分析16尾华鲮(Sinilabeo rendahli)的肌肉营养成分。结果表明:华鲮肌肉(鲜样)中各成分的质量分数分别是蛋白质16.69%,脂肪2.91%,水分77.90%,灰分1.82%,无氮浸出物0.68%。肌肉中含有19种氨基酸,总量为56.52%(质量分数,脱脂干样),其中8种人体必需氨基酸总量是25.81%,占氨基酸总量的45.67%;其必需氨基酸的构成比例基本符合FAO/WHO的标准。华鲮的必需氨基酸指数(EAAI)为57.37。脂肪酸中EPA与DHA质量分数分别为7.41%、28.53%,均比其他一些经济鱼类高,微量元素比值合理。  相似文献   

中华沙鳅肌肉营养成分分析及营养学评价   总被引：1，自引：0，他引：1  

何斌  陈先均  龙治海  朱成科  温涛  严俊刚 《淡水渔业》2013,43(3):93-96

采用常规生化分析方法对中华沙鳅(Botia superciliaris Günther)肌肉成分进行了测定。结果表明:中华沙鳅含肉率为(74.89±1.59)%,肌肉(鲜样)中水分、粗蛋白、粗脂肪和粗灰分的质量分数分别为(76.65±1.37)%、(20.05±0.87)%、(1.56±0.12)%和(1.34±0.09)%。肌肉中含有18种氨基酸,肌肉(鲜样)中氨基酸、必需氨基酸(EAA)和鲜味氨基酸(DAA)含量分别为(19.42±0.07)%、(8.27±0.08)%和(7.13±0.06)%。肌肉中含有17种脂肪酸,肌肉(干样)中多不饱和脂肪酸(PUFA)含量为(1.96±0.06)%。  相似文献   

Ureolytic Activity of Soybean and Corn Residue Extracts     

《Communications in Soil Science and Plant Analysis》2012,43(22):2959-2969

The enzyme urease is responsible for the rapid hydrolysis of urea in agroecosystems where more than 50% of the applied nitrogen (N) can be lost via ammonia volatilization. The objectives of the study were to (1) extract urease from corn (Zea mays L.) and soybean (Glycine max L.) and (2) compare the urease activity from soybean and corn residues to Jackbean (Canavalia ensiformis) urease using Fourier transform infrared spectroscopy (FTI-R). Concentrations of urea and sodium bicarbonate ranging from 0 to 200 mM were analyzed with FTI-R to determine the correlation to peak height. Bicarbonate produced the most responsive peaks to concentration at 1,362 cm^?1. Urease extracted from soybean residue was active, producing a bicarbonate peak at 1362 cm^?1, whereas no urease activity was observed in corn residue. Variation among urease activities in crop residues could suggest a need for more precise nitrogen management in conservation tillage agroecosystems to reduce ammonia volatilization.  相似文献   

施肥方式对蔬菜地土壤中8种抗生素残留的影响     

鲍陈燕  顾国平  徐秋桐  章明奎 《农业环境与发展》2014,(4)

从浙江省杭州、嘉兴和绍兴等3个地级市采集了4种不同施肥方式下(分别为施用畜禽粪+化肥、商品有机肥+化肥、沼渣+化肥和单施化肥)的蔬菜地表层土壤样品44个,分析了4类8种抗生素(包括四环素类抗生素的土霉素、四环素和金霉素,喹诺酮类抗生素的恩诺沙星,磺胺类抗生素的磺胺嘧啶、磺胺二甲嘧啶和磺胺甲噁唑及大环内脂类抗生素的泰乐菌素)的残留情况,探讨了施肥方式对蔬菜地土壤中抗生素残留的影响。结果表明,蔬菜地土壤中抗生素的检出率和残留含量与施肥方式密切相关。8种检测的抗生素中土霉素的检出率和残留含量明显高于其他种类的抗生素,土霉素的平均含量占8种抗生素总量平均值的67.03%。抗生素的检出率和平均含量由高至低依次为:土霉素磺胺二甲嘧啶恩诺沙星四环素磺胺甲噁唑、泰乐菌素金霉素磺胺嘧啶;四环素类抗生素磺胺类抗生素。土壤中各类抗生素的检出率及含量均为施用畜禽粪的蔬菜地施用商品有机肥的蔬菜地施用沼渣的蔬菜地单施化肥的蔬菜地,施用畜禽粪的蔬菜地土壤中抗生素残留量明显高于其他蔬菜地。试验结果表明,畜禽粪是蔬菜地土壤抗生素的主要来源,商品有机肥和沼渣的施用对蔬菜地土壤中抗生素的残留也有一定的贡献。  相似文献   

群居型和散居型亚洲小车蝗成虫能量代谢相关酶活性测定     

高书晶  闫锋  郭娜  王宁  韩海斌  林克剑  贺兵 《植物保护学报》2021,48(1):144-151

为明确亚洲小车蝗Oedaleus asiaticus成虫飞行肌对其体内能源物质利用的情况,采用室内生化法测定能源物质代谢相关5种酶,即3-磷酸甘油醛脱氢酶(glyceraldehyde phosphate dehydrogenase,GAPDH)、3-磷酸甘油脱氢酶(glycerol-3-phosphate dehyd...  相似文献   

纤维素超低酸水解产物的分析   总被引：16，自引：2，他引：16  

庄新姝  王树荣  袁振宏  骆仲泱  吴创之  岑可法 《农业工程学报》2007,23(2):177-182

基于木质纤维素类生物质水解为人类提供乙醇等能源、化工产品的重要性，该文以定量滤纸模拟生物质的主要组分－纤维素，以其超低酸水解试验得到的最佳工况下液体产物和固体残渣为研究对象，通过高效液相色谱(HPLC)结合KS-802糖柱对产物质中糖的种类进行了划分，以GC-MS对副产物进行定性，并通过扫描电镜(SEM)及热重和工业分析元素分析对固体残渣作了从表观到内部的研究，发现纤维素超低酸水解主要生成纤维四糖、三糖和二糖等低聚糖和葡萄糖及果糖，反应副产物有糠醛、羟甲基糠醛、乙酰丙酸及一些小分子酮、醛类和酸类极性化合物，水解残渣已完全改变了原始形貌，热裂解活性增强，残留物中碳和灰分含量有较大增加，最后根据分析结果探讨了纤维素超低酸水解的反应途径，为木质纤维素类生物质水解技术的规模化利用打下基础。  相似文献