A survey of wild marine fish identifies a potential origin of an outbreak of viral haemorrhagic septicaemia in wrasse,Labridae, used as cleaner fish on marine Atlantic salmon,Salmo salar L., farms          下载免费PDF全文

I S Wallace  K Donald  L A Munro  W Murray  C C Pert  H Stagg  M Hall  N Bain 《Journal of fish diseases》2015,38(6):515-521

Viral haemorrhagic septicaemia virus (VHSV) was isolated from five species of wrasse (Labridae) used as biological controls for parasitic sea lice predominantly, Lepeophtheirus salmonis (Krøyer, 1837), on marine Atlantic salmon, Salmo salar L., farms in Shetland. As part of the epidemiological investigation, 1400 wild marine fish were caught and screened in pools of 10 for VHSV using virus isolation. Eleven pools (8%) were confirmed VHSV positive from: grey gurnard, Eutrigla gurnardus L.; Atlantic herring, Clupea harengus L.; Norway pout, Trisopterus esmarkii (Nilsson); plaice, Pleuronectes platessa L.; sprat, Sprattus sprattus L. and whiting, Merlangius merlangus L. The isolation of VHSV from grey gurnard is the first documented report in this species. Nucleic acid sequencing of the partial nucleocapsid (N) and glycoprotein (G) genes was carried out for viral characterization. Sequence analysis confirmed that all wild isolates were genotype III the same as the wrasse and there was a close genetic similarity between the isolates from wild fish and wrasse on the farms. Infection from these local wild marine fish is the most likely source of VHSV isolated from wrasse on the fish farms.  相似文献   

First report of Erysipelothrix rhusiopathiae‐associated septicaemia and histologic changes in cultured Australian eels,Anguilla reinhardtii (Steindachner, 1867) and A. australis (Richardson, 1841)          下载免费PDF全文

R S‐M Chong  M W Shinwari  M J Amigh  M Aravena‐Roman  T V Riley 《Journal of fish diseases》2015,38(9):839-847

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A mortality event in wrasse species (Labridae) associated with the presence of viral haemorrhagic septicaemia virus          下载免费PDF全文

E S Munro  R E McIntosh  S J Weir  P A Noguera  J M Sandilands  I Matejusova  A S Mayes  R Smith 《Journal of fish diseases》2015,38(4):335-341

Viral haemorrhagic septicaemia (VHS) is an infectious disease of farmed and wild fish and has an extensive host range in both freshwater and marine environments. In December 2012, a wrasse population consisting of ballan, Labrus bergylta (Ascanius), corkwing, Symphodus melops (L.), cuckoo, Labrus mixtus L., goldsinny, Ctenolabrus rupestris (L.), and rock cook, Centrolabrus exoletus (L.), held at a marine hatchery in the Shetland Isles, Scotland, experienced a mortality event. Approximately 10 000 wrasse were being held at the facility on behalf of an Atlantic salmon, Salmo salar L., aquaculture company prior to being deployed for the biological control of parasites on marine pen Atlantic salmon, aquaculture sites. Fish Health Inspectors from Marine Scotland Science initiated a diagnostic investigation, and subsequent diagnostic testing confirmed the site to be VHSV positive by qRT-PCR and virus isolation followed by ELISA. A VHSV genotype-specific qRT-PCR assay revealed that the isolates belonged to genotype III, the European marine strain of the virus. The virus genotype was further confirmed by nucleic acid sequencing of the partial nucleoprotein (N) and glycoprotein (G) genes followed by BLAST nucleotide searches. This study reports for the first time the detection of VHSV within multiple wrasse species and highlights the need for a comprehensive risk-based approach to the use of wrasse and other finfish species as biological controls within the aquaculture industry.  相似文献   

Edwardsiella piscicida‐associated septicaemia in a blotched fantail stingray Taeniura meyeni (Müeller & Henle)          下载免费PDF全文

A Camus  J Dill  A McDermott  N Hatcher  M Griffin 《Journal of fish diseases》2016,39(9):1125-1131

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Fish viruses make dsRNA in fish cells: characterization of dsRNA production in rainbow trout (Oncorhynchus mykiss) cells infected with viral haemorrhagic septicaemia virus,chum salmon reovirus and frog virus 3          下载免费PDF全文

L Doherty  S J Poynter  A Aloufi  S J DeWitte‐Orr 《Journal of fish diseases》2016,39(9):1133-1137

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致鹅败血症粪肠球菌的分离鉴定     

狄婷婷  高原  聂鑫  马万欣  董国军  曾钰然 《中国预防兽医学报》2012,34(3):192-196

为确定导致通辽某鹅场雏鹅败血症的病原,本研究将从病鹅心、肝、淋巴结和脑组织液中分离的革兰氏阳性球菌纯化培养,进行形态学和生长耐受性观察,菌属区别、分群和菌种鉴定试验,16S rDNA检测、系统进化分析和致病性试验。结果表明,分离菌株(HQ831431)为中等致病力粪肠球菌,具有β型溶血特性,可致小鼠急性败血症。分离菌株与参考菌株ATCC29212及国内外分离的其他15株粪肠球菌16S rDNA的同源性均在99.0%以上,位于系统发育树的同一分支。  相似文献   

Actinobacillus suis infection in pigs in Australia     

Wilson RJ  McOrist S 《Australian veterinary journal》2000,78(5):317-319

This is the first report of the isolation of Actinobacillus suis in association with significant disease among preweaned pigs in Australia. Sudden deaths occurred in preweaned piglets at one facility and enlarged joints, particularly the stifles and tarsi, occurred at another. Isolates with the biochemical phenotype and apx genotype profile of A suis were cultured from affected piglets. Both facilities were of high health status and one had undergone a recent depopulation, disinfection and sow repopulation process. Reviewing initial reports of A suis disease overseas, it is apparent that outbreaks occurred sporadically in various locations, with disease occurring among the herds involved for some months only, before development of immunity. A suis disease is now considered an emerging disease on high health status farms.  相似文献   

Investigation of round goby viral haemorrhagic septicaemia outbreak in New York     

Rodman G. Getchell  Erika J. First  Steven M. Bogdanowicz  Jose A. Andrs  Adam T. Schulman  Jordan Kramer  Geofrey E. Eckerlin  John M. Farrell  Hlne Marquis 《Journal of fish diseases》2019,42(7):1023-1033

Eleven viral haemorrhagic septicaemia virus (VHSV) genotype IVb isolates were sequenced, and their genetic variation explored to determine the source of a VHS outbreak on the eastern shore of Cayuga Lake. An active fish kill of round gobies (Neogobius melanostomus, Pallas) was intensively sampled at King Ferry, NY and nearby Long Point State Park in May 2017. Gross lesions observed on 67 moribund round gobies and two rock bass (Ambloplites rupestris, Rafinesque) included moderately haemorrhagic internal organs and erythematous areas on the head, flank, and fins. RT‐qPCR tests for VHSV were positive for all 69 fish. Viral isolation on epithelioma papulosum cyprinid cells showed cytopathic effect characteristic of VHSV for six round goby samples from King Ferry. The complete nucleotide sequence of the VHSV IVb genomes of five Cayuga Lake round goby isolates were derived on an Illumina platform along with 2017 VHSV IVb isolates from round gobies collected from the following: Lake Erie near Dunkirk, NY; the St. Lawrence River near Clayton and Cape Vincent, NY; and Lake St. Lawrence near Massena, NY. The phylogenetic tree created from these aligned sequences and four other complete VHSV IVb genomes shows Cayuga Lake isolates are closely related to the Lake Erie isolates.  相似文献   

Establishment and characterization of a fin tissue cell line derived from silver pomfret,Pampus argenteus     

Jianhuan Li  Peng Jia  Xueji Chen  Mingyan Lai  Fanming Jin  Wei Liu  Meisheng Yi  Kuntong Jia 《Journal of fish diseases》2019,42(10):1391-1399

A cell line (PaF) derived from the fin tissue of silver pomfret (Pampus argenteus) was established and characterized in this study. The cell line has been subcultured for more than 50 times in Dulbecco's modified Eagle's medium (DMEM) containing 15% foetal bovine serum (FBS) since the initial primary culture. PaF cells grew well at temperatures from 24°C to 28°C in DMEM supplemented with 15% FBS. Partial amplification and sequence analysis of the cytochrome B gene indicated that PaF originated from silver pomfret. Cytogenetic analysis demonstrated that the modal chromosome number was 48. A significant cytopathic effect was observed in PaF cells during viral haemorrhagic septicaemia virus (VHSV) infection, and the VHSV replication was confirmed by qRT‐PCR and viral titre assays. In contrast, PaF cells were resistant to red‐spotted grouper nervous necrosis virus infection. Moreover, PaF cells could respond to VHSV and lipopolysaccharide treatments, as indicated by the expression of immune‐related genes, TLR5 and TLR9. In conclusion, the establishment of PaF cell line will provide an appropriate in vitro tool for the study of mechanisms of pathogen–silver pomfret interaction.  相似文献   

Experimental studies confirm the wide host range of the Great Lakes viral haemorrhagic septicaemia virus genotype IVb     

R Kim  M Faisal 《Journal of fish diseases》2010,33(1):83-88

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