BCR-ABL translocation in a dog with chronic monocytic leukemia     

Cruz Cardona JA  Milner R  Alleman AR  Williams C  Vernau W  Breen M  Tompkins M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2011,40(1):40-47

A 9-year-old female spayed mixed breed dog was evaluated at the University of Florida Small Animal Hospital for marked leukocytosis with no associated clinical signs. CBC abnormalities included marked leukocytosis (106,000/μL), marked monocytosis (78,000/μL), and the presence of 13% blast cells (13,832/μL), supporting a diagnosis of leukemia. Cytopenias and dysplastic changes in other cell lines were not present. Microscopic examination of bone marrow showed hypercellular uniparticles with a marginal increase in frequency of unclassified blast cells (2%), but was otherwise unremarkable. Flow cytometric immunophenotyping of blood cells determined that leukemic cells were CD45(+) , CD14(+) , and CD34(-) , and based on side scatter and CD45 reactivity the marrow contained 19% monoblasts. By immunocytochemical staining, the leukemic cells in the bone marrow were CD11b(+) , CD11c(+) , CD11d(+) , MHC-II(+) , MPO(+) , and CD34(-) . Fluorescence in situ hybridization (FISH) analysis of peripheral blood leukocytes documented a chromosomal translocation producing a BCR-ABL gene hybrid, similar to the "Philadelphia" chromosome abnormality recognized in human chronic myelogenous leukemia, as well as a phosphatase and tensin homolog (PTEN) gene deletion. Hydroxyurea therapy was attempted, but was ineffective; the dog died 7 months after initial presentation. Clinical and laboratory findings and the protracted course supported a diagnosis of chronic monocytic leukemia (CMoL) and, to our knowledge, this is the first case of CMoL with a BCR-ABL chromosomal abnormalitiy described in dogs. This may have clinical implications for treatment of dogs with chronic leukemias associated with particular genetic mutations. However, more case studies are needed to further characterize this disease.  相似文献   

45S rDNA在蒿属5种植物染色体中的分布     

李真  陈素梅  陈发棣  王海滨  汤访评  刘兆磊  房伟民 《园艺学报》2011,38(2):353

 以小麦45S rDNA为探针,通过荧光原位杂交（FISH）技术,研究了45S rDNA在蒿属（Artemisia）5个物种染色体中的分布情况。结果发现,在根尖体细胞有丝分裂中期,中亚苦蒿（A. absinthum Linn.）有1对染色体出现杂交信号;大籽蒿（A. sieversiana Ehrhart ex Willd.）和黄花蒿（A. annua Linn.）各有两对染色体上出现信号;欧亚艾蒿（A. abrotanum Linn.）和牡蒿（A. japonica Thumb.）各有4对染色体出现杂交信号。信号位点除了分布在随体上,主要分布在非随体染色体的端部,而在欧亚艾蒿中发现1对信号位点分布在近端部,而且信号间的强弱也不尽相同。基于FISH技术的核型分析,不仅能够提供稳定、可靠的细胞学标记,而且进一步丰富了染色体分析的基础资料。  相似文献   

中国水仙3个特异种质的分子细胞遗传学分析   总被引：1，自引：0，他引：1  

吴菁华  张志忠  吕柳新 《园艺学报》2011,38(4):740-746

 应用荧光原位杂交技术进行rDNA在黄花水仙和两色花水仙染色体上的定位研究,并分析了两色花水仙的基因组成。结果表明：不同材料的NOR位点表现出多态性,即黄花水仙上具3个45S rDNA位点,两色花水仙具有5个位点;5S rDNA在位点和数量上表现出一致性。基因组荧光原位杂交结果表明两色花水仙的染色体有10条来自于黄花水仙,22条来自于白花水仙。根据rDNA在这些种质资源上的分布,结合GISH技术的分析,证实两色花水仙是黄花水仙和白花水仙的天然杂交种。  相似文献   

黑麦特异DNA重复序列的分离与鉴定   总被引：14，自引：0，他引：14  

周建平  杨足君  冯娟  唐宗祥  任正隆 《西南农业学报》2005,18(5):598-602

为了找到黑麦特异的DNA重复序列，我们采用随机扩增多态性DNA（RAPD）标记对黑麦、小麦及其他麦类植物材料分析发现引物OPH20在所有黑麦中扩增出特异的两条带。将这两条带DNA回收克隆测序，得到两序列的长度分别为1495bp、1147bp，分别命名为pSc20H．1、pSc20H．2。序列比对发现pSc20H．1的序列与已报道的序列pSc20n（GenBank编号AF305943）一致达到97％。没有与pSc20H．2高度一致的同源序列。荧光原位杂交结果显示pSc20H．1分布黑麦所有染色体的除端部和核仁组织区域的所有部位。pSc20H．2也分布在黑麦所有染色体上，但分布区域不同于pSC20H．1。证明了pSc20H．2是黑麦新的特异DNA序列，并可用于检测小麦背景中的黑麦染色体成分。  相似文献   

内麦系列小麦品种(系)的染色体结构变异分析     

关淑仙  黄辉跃  汪仁全  王相权  王仕林  荣飞雪  杨杰智  钟光跃  周海燕  陈新媛  李明  黄书盈 《麦类作物学报》2021,(6):673-679

为明确内麦系列小麦品种(系)的染色体结构特点,利用荧光原位杂交(fluorescence in situ hybridization, FISH)技术和pTa535、pSc119.2探针对21份内麦系列小麦材料的染色体进行分析。结果显示,5份材料含1RS/1BL易位染色体;探针除3A、7A、2B、3B、4B、2D和4D外的其他染色体在21份材料间存在结构差异;共鉴定出49种染色体多态类型,其中数量最多的是A基因组(22),其次是B基因组(14)和D基因组(13);在5B、6B、3D和7D染色体的同源染色体间存在不对称核型;根据A、B和D基因组的FISH核型,把21份材料分为16类。通过FISH信号模式,发现所有材料在染色体水平上都有变异。多数材料的染色体类型一致,推测这些材料的遗传背景一致或者相似。  相似文献   

Association between bacterial community structures and mortality of fish larvae in intensive rearing systems     

Gentoku Nakase  Yoshizumi Nakagawa  Shigeru Miyashita  Toshiro Nasu  Shigeharu Senoo  Hiroko Matsubara  Mitsuru Eguchi 《Fisheries Science》2007,73(4):784-791

Bacterial community structures were analyzed in water used for rearing fish larvae by fluorescence in situ hybridization. In Experiment 1, red sea bream Pagrus major larvae were reared in two commercial seed production tanks. The survival rate in Tank 1 was higher than in Tank 2, even though phytoplankton, Nannochloropsis sp., was added to both tanks. In Tank 2, γ-proteobacteria became dominant (∼70% of total bacteria) on day 13, there after heavy larval mortalities occurred. In Tank 1, however, α-proteobacteria and the Cytophaga-Flavobacterium cluster were predominant from day − 1 until day 13; no significant mortality was recorded. In Experiment 2, marble goby Oxyeleotris marmoratus larvae were cultured with or without Nannochloropsis sp. At the end of the experiment, larval survival rates in aquaria with Nannochloropsis sp. were significantly (P <0.05) higher than those without. In rearing water without Nannochloropsis sp., γ-proteobacteria increased during rearing. In rearing water with Nannochloropsis sp., α-prote obacteria and the Cytophaga-Flavobacterium cluster were predominant at the beginning of the experiments and the relative abundance of γ-proteobacteria was maintained at a lower level throughout the experiments. The predominance of α-proteobacteria and the Cytophaga-Flavobacterium cluster appears to be a good indicator of successful larval production.  相似文献   

西藏开菲尔粒中酵母菌的分离与初步鉴定   总被引：1，自引：1，他引：0  

卢曼  陈小兵  王兴兴  潘迎捷  王永杰 《上海海洋大学学报》2015,24(1):138-145

西藏开菲尔粒(俗称藏灵菇)是由多种乳酸菌(间或少量醋酸菌)和酵母菌共生而成一种外形酷似菜花的白色或乳黄色团状聚合体,是目前已知的唯一可用于酸奶制作的天然野生菌种资源。采用扫描电镜、分离培养、26S rRNA基因测序与序列分析、荧光原位杂交(FISH)相结合的技术方法,对西藏开菲尔粒中的酵母菌进行了分离培养、鉴定和形态观察。结果显示:西藏开菲尔粒中酵母菌多为球形(2.0~5.0μm)或椭圆形(1.0~3.0×2.0~4.0μm);从西藏开菲尔粒中分离出的16株酵母菌分属于酿酒酵母属(Saccharomyces)、克鲁维酵母属(Kluyveromyces)、亚罗酵母属(Yarrowia)这3个属中的4个种,其中3株是酿酒酵母菌(Saccharomyces cerevisiae),8株为马克斯克鲁维酵母菌(Kluyveromyces marxianus),3株为解脂亚罗酵母菌(Yarrowia lipolytica)。  相似文献   

转外源基因(pGH)在猪染色体上整合状况     

蒋亚军  吴明明  孙金海 《中国兽医学报》2013,33(7)

应用地高辛标记技术,对通过睾丸注射法制备的转基因猪进行荧光原位杂交,确定外源pGH(猪生长激素)基因在染色体上的整合位点及数目.选用睾丸注射法制备的140日龄转基因长白猪6头与同期同龄非转基因猪2头进行血细胞培养及荧光原位杂交.结果显示,外源基因已整合于染色体上,但在染色体上的整合位点具有随机性,而且不同的转基因猪阳性个体外源基因在染色体上的整合位点有差异,但集中分布于1、6、8、13号染色体上;非转基因猪染色体上无杂交信号;个体杂交信号数越多,其生长速度越快.  相似文献   

Development and characterization of new allohexaploid resistant to web blotch in peanut          下载免费PDF全文

Si-yu WANG  Li-na LI  Liu-yang FU  Hua LIU  Li QIN  Cai-hong CUI  Li-juan MIAO  Zhong-xin ZHANG  Wei GAO  Wen-zhao DONG  Bing-yan HUANG  Zheng ZHENG  Feng-shou TANG  Xin-you ZHANG  Pei DU 《农业科学学报》2021,20(1):55-64

Peanut diseases seriously threaten peanut production, creating disease-resistant materials via interspecific hybridization is an effective way to deal with this problem. In this study, the embryo of an interspecific F_1 hybrid was obtained by crossing the Silihong(Slh) cultivar with Arachis duranensis(ZW55), a diploid wild species. Seedlings were generated by embryo rescue and tissue culture. A true interspecific hybrid was then confirmed by cytological methods and molecular markers. After treating seedlings with colchicine during in vitro multiplication, the established interspecific F_1 hybrid produced seeds which were named as Am1210. With oligonucleotide fluorescence in situ hybridization(Oligo FISH), molecular marker evaluations, morphological and web blotch resistance characterization, we found that: 1) Am1210 was an allohexaploid between Slh and ZW55; 2) the traits of spreading lateral branches, single-seeded or double-seeded pods and red seed coats were observed to be dominant compared to the erect type, multiple-seeded pods and brown seed coats; 3) the web blotch resistance of Am1210 was significantly improved than that of Slh, indicating the contribution of the web blotch resistance from the wild parent A. duranensis. In addition, 69 dominant and co-dominant molecular markers were developed which could be both used to verify the hybrid in this study and to identify translocation or introgression lines with A. duranensis chromosome fragments in future studies as well.  相似文献   

巴西橡胶树MADS-box基因家族6个成员的染色体物理定位     

陶志强  张宇航  王英  高和琼  庄南生 《热带作物学报》2020,41(12):2365-2371

MADS-box基因家族广泛分布于真核生物中,巴西橡胶树的MADS-box基因家族主要参与花形态建成,对生殖生长起到重要的调节作用。目前,MADS-box基因家族的26个相关基因已被克隆分析,但它们在染色体上的具体位置还未确定。本研究以巴西橡胶树‘热研7-33-97’品种为材料,将MADS-box基因家族的6个成员（HbAGL8、HbAG15、HbAGL30、HbTT16、HbAP1和HbSVP1）定位在细胞核染色体上,通过双探针荧光原位杂交技术（FISH）对巴西橡胶树MADS-box基因家族的这6个成员在细胞核染色体上进行物理定位分析。结果表明：MADS-box基因家族的6个基因分别位于不同的染色体上,其中HbAGL15、HbAG8、HbAG30和HbSVP1基因定位在第4、5、7和8号染色体长臂上,其信号位点到着丝粒的平均百分距离是11.85、39.71、48.94和6.70;HbTT16和HbAP1基因定位在第1和13号染色体短臂上,其信号位点到着丝粒的平均百分距离是22.19和18.01。本研究结果揭示了巴西橡胶树MADS-box基因家族的6个成员在细胞核染色体上的实际位置,展现家族基因之间的分布特点和连锁遗传关系,不仅丰富了橡胶树分子细胞遗传学信息,也为橡胶树的分子辅助育种和比较基因组学研究提供了分子细胞遗传学的科学理论依据。  相似文献