Allele distributions of two novel SNPs within the sheep Cyp19 gene     

Attila Zsolnai  István Anton  László Fésüs  one Estonba  Manfred Schwerin  Jens Vanselow 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2002,119(6):402-405

Oestrogen is an important regulator of reproduction and growth. The key enzyme of oestrogen biosynthesis, aromatase cytochrome P450, is encoded by the Cyp19 gene. In order to generate genetic markers for the sheep Cyp19 gene, two novel single nucleotide polymorphisms (SNPs), one located in promoter 2 (P2), the other one in intron 9 (I9), were identified by a comparative sequencing approach. The allele distributions of both SNPs were investigated by means of Polymerase chain reaction‐restriction fragment length polymorphism analysis (PCR‐RFLP) in five economically relevant sheep breeds (British Milk Sheep, Carranzana, Latxa Black Face, Latxa White Face, Merino) and three ancient Hungarian breeds kept as gene reserves (Cikta, Racka, Tsigai). In British Milk Sheep, only the intronic SNP was present whereas in Merino, Cikta, Racka, Tsigai, Carranzana, Latxa Black Face and Latxa White Face, both SNPs could be found. This indicates that the newly identified SNPs can be used as markers for the Cyp19 locus in various sheep breeds.  相似文献   

The roles of subsurface carbon and hydrogen in palladium-catalyzed alkyne hydrogenation     

Teschner D  Borsodi J  Wootsch A  Révay Z  Hävecker M  Knop-Gericke A  Jackson SD  Schlögl R 《Science (New York, N.Y.)》2008,320(5872):86-89

Alkynes can be selectively hydrogenated into alkenes on solid palladium catalysts. This process requires a strong modification of the near-surface region of palladium, in which carbon (from fragmented feed molecules) occupies interstitial lattice sites. In situ x-ray photoelectron spectroscopic measurements under reaction conditions indicated that much less carbon was dissolved in palladium during unselective, total hydrogenation. Additional studies of hydrogen content using in situ prompt gamma activation analysis, which allowed us to follow the hydrogen content of palladium during catalysis, indicated that unselective hydrogenation proceeds on hydrogen-saturated beta-hydride, whereas selective hydrogenation was only possible after decoupling bulk properties from the surface events. Thus, the population of subsurface sites of palladium, by either hydrogen or carbon, governs the hydrogenation events on the surface.  相似文献   

High-accuracy ab initio rotation-vibration transitions for water     

Polyansky OL  Császár AG  Shirin SV  Zobov NF  Barletta P  Tennyson J  Schwenke DW  Knowles PJ 《Science (New York, N.Y.)》2003,299(5606):539-542

The spectrum of water vapor is of fundamental importance for a variety of processes, including the absorption and retention of sunlight in Earth's atmosphere. Therefore, there has long been an urgent need for a robust and accurate predictive model for this spectrum. In our work on the high-resolution spectrum of water, we report first-principles calculations that approach experimental accuracy. To achieve this, we performed exceptionally large electronic structure calculations and considered a variety of effects, including quantum electrodynamics, which have routinely been neglected in studies of small many-electron molecules. The high accuracy of the resulting ab initio procedure is demonstrated for the main isotopomers of water.  相似文献   

Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk     

Abtahian F  Guerriero A  Sebzda E  Lu MM  Zhou R  Mocsai A  Myers EE  Huang B  Jackson DG  Ferrari VA  Tybulewicz V  Lowell CA  Lepore JJ  Koretzky GA  Kahn ML 《Science (New York, N.Y.)》2003,299(5604):247-251

Lymphatic vessels develop from specialized endothelial cells in preexisting blood vessels, but the molecular signals that regulate this separation are unknown. Here we identify a failure to separate emerging lymphatic vessels from blood vessels in mice lacking the hematopoietic signaling protein SLP-76 or Syk. Blood-lymphatic connections lead to embryonic hemorrhage and arteriovenous shunting. Expression of slp-76 could not be detected in endothelial cells, and blood-filled lymphatics also arose in wild-type mice reconstituted with SLP-76-deficient bone marrow. These studies reveal a hematopoietic signaling pathway required for separation of the two major vascular networks in mammals.  相似文献   

Further data to the aetiology, pathogenesis and therapy of infectious bovine keratoconjunctivitis     

Attila Nagy  mile Vandersmissen  Pl Kapp 《Comparative immunology, microbiology and infectious diseases》1989,12(4):115-127

Out of a total of 224 bovine eye secretions, 126 Moraxella bovis and 64 Neisseria ovis strains were isolated. The pathogenesis and histological lesions caused by Neisseria ovis have been studied on the eyes of three calves naturally affected with IBK, using electron microscopy.

Neisseria ovis caused in 1–12 weeks old calves acute, transient and mostly benign serous conjunctivitis with only slight affection of the cornea. More rarely erosions and even ulceration of the cornea have been observed.

Moraxella bovis and Neisseria ovis strains proved nearly unanimously sensitive in vitro to chloramphenicol, neomycin, oxytetracyclin, nitrofurantoin, erythromycin and cefoperazone. Other antibiotics and chemotherapeutics inhibited the growth of these agents only partly or were ineffective.

Experimental therapy has been carried out using a single i.m. injection of Terramycin/LA inj. (Pfizer) in a dose of 20 mg/kg body mass, repeated if necessary after 72–96 h. This formulation proved more effective and practical than treatments used earlier.  相似文献   

Replication and transmission of porcine circovirus type 2 in mice     

Cságola A  Cadar D  Tuboly T 《Acta veterinaria Hungarica》2008,56(3):421-427

Little information is known about infection, replication and transmission of porcine circovirus type 2 (PCV2) in species other than swine. Two sets of animal experiments were carried out to investigate the susceptibility of mice to PCV2 and to study their possible role in maintaining and transmitting the virus. In the first experiment 14 mice were inoculated with PCV2 by the intraperitoneal route with 5 x 10(2) TCID50 of the PCV2-ROM strain (Cadar et al., 2007). In a second experiment 24 mice were divided into two groups (A and B); mice in Group A (n = 18) were inoculated orally with 1 x 10(5) TCID50 PCV2-ROM and mice in Group B (n = 6) were left uninoculated until day 12 post inoculation (p.i.), when they were mixed with Group A. The animals were sacrificed at intervals for postmortem investigation and virus genome detection by polymerase chain reaction (PCR). The PCR results indicated that PCV2 could replicate in mice infected intraperitoneally or by the oral route, and that the virus can be transmitted directly from mouse to mouse.  相似文献   

ULTRASONIC DIAGNOSIS OF OVARIAN CYSTS IN TEN GUINEA PIGS     

Attila  Beregi  DVM  Susanne  Zorn  Vet Med Student  Ferenc  Felkai  DVM PhD 《Veterinary radiology & ultrasound》1999,40(1):74-76

Ten guinea pigs with an ovarian cyst had clinical signs of anorexia, alopecia, or depression. Ultrasonographic features of the 2- to 3-cm diameter fluid-filled cysts included compartmentalization and connection to the ovary. In two animals, a unilateral cyst was present, and in two others, the cysts were bilateral. Diagnosis of the disease by plain radiography is difficult because of the similar opacity of ovarian cysts, abdominal neoplasms, and trichobezoars.  相似文献   

Ethnobotanical and ethnopharmacological data of <Emphasis Type="Italic">Armoracia rusticana</Emphasis> P. Gaertner,B. Meyer et Scherb. in Hungary and Romania: a case study     

Nóra Papp  Sándor Gonda  Attila Kiss-Szikszai  Tamás Plaszkó  Péter Lőrincz  Gábor Vasas 《Genetic Resources and Crop Evolution》2018,65(7):1893-1905

Horseradish (Armoracia rusticana) is a popular vegetable, spice and medicinal plant. We aimed to collect data about its cultivation, ethnomedicinal and culinary applications in a horseradish-producing region in Hungary and 32 villages in Romania. Horseradish roots are used in ethnomedicine for sore throat, asthma, cough and flu in both areas, alone or in combination with e.g. honey. In Transylvania, additional applications included digestive problems, pleuritis, and rheuma in various forms. The leaf and the grated root is for toothache and fever. Some applications were novel, others matched earlier records from these areas and those of other European countries. In ethnoveterinary practice, the root is used against roaring of horses in all regions. It is traditionally consumed in a soup in Hungary, as pickles, and the leaf in Transylvania. The root samples of the region of interest showed typical chemical pattern of horseradish roots, as shown by GC–MS: allyl isothiocyanate and 2-phenylethyl isothiocyanate were present in high amounts. The presented ethnomedicinal data are supported by a growing body of scientific evidence—pharmacological data available on the isothiocyanates. Data on antimicrobial activity support use in gastritis (Helicobacter pylori) and dental caries, while data on efficacy in inflammatory conditions justify the applications in diseases asthma and respiratory system infections. Our study presented scientific evidence-supported ethnomedicinal data on horseradish rich in bioactive isothiocyanates.  相似文献   

Thermoformed wheat gluten biopolymers     

Pallos FM  Robertson GH  Pavlath AE  Orts WJ 《Journal of agricultural and food chemistry》2006,54(2):349-352

The quantity of available wheat gluten exceeds the current food use markets. Thermoforming is an alternative technical means for transforming wheat gluten. Thermoforming was applied here to wheat gluten under chemically reductive conditions to form pliable, translucent sheets. A wide variety of conditions, i.e., temperature, reducing agents, plasticizers and additives were tested to obtain a range of elastic properties in the thermoformed sheets. These properties were compared to those of commercially available polymers, such as polypropylene. Elasticity of the gluten formulations were indexed by Young's modulus and were in the range measured for commercial products when tested in the 30-70% relative humidity range. Removal of the gliadin subfraction of gluten yielded polymers with higher Young's modulus since this component acts as a polymer-chain terminator. At relative humidity less than 30% all whole gluten-based sheets were brittle, while above 70% they were highly elastic.  相似文献   

Prevalence of porcine circoviruses in Transylvanian wild boars, detected by real-time PCR--short communication     

Cadar D  Cságola A  Spinu M  Dán A  Ursu K  Lorincz M  Tuboly T 《Acta veterinaria Hungarica》2010,58(4):475-481

Porcine circoviruses (PCV) are widespread in domestic pigs worldwide and there is growing information about the presence of PCV in other suid species. Based on serological studies with sera of wild boars, it was established that PCV1 was present in these animals and antibodies specific to PCV2 were also detected in wild boars living in captivity or in sylvatic areas, both with or without clinical signs of PMWS. Studies including PCV2 genome or antigen detection confirmed the previous findings. This is the first report about the presence of PCV in Transylvanian wild boar populations. Four hundred and sixty-nine samples were collected and grouped according to geographic origin, tested for the presence of PCV DNA using a real-time quantitative polymerase chain reaction assay, and 13.52% of the animals proved to be positive for one or in three cases both of the PCV genotypes. PCV2 was detected in all of the PCV-positive samples.  相似文献