Production of Lambs After the Transfer of Fresh and Cryopreserved in vitro Produced Embryos from Prepubertal Lamb Oocytes and Unsorted and Sex-sorted Frozen-thawed Spermatozoa     

KM Morton  SL Catt  FK Hollinshead  WMC Maxwell  G Evans 《Reproduction in domestic animals》2004,39(6):454-461

Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm.  相似文献   

Prevalence of Leptospira serogroup-specific antibodies in cattle associated with reproductive problems in endemic states of India     

Balamurugan  Vinayagamurthy  Alamuri  Anusha  Bharathkumar  K.  Patil  Sharanagouda Siddanagouda  Govindaraj  Gurrappa Naidu  Nagalingam  Mohandoss  Krishnamoorthy  Paramanandham  Rahman  Habibur  Shome  Bibek Ranjan 《Tropical animal health and production》2018,50(5):1131-1138

Tropical Animal Health and Production - In this study, the seroprevalence and distribution of Leptospira in dairy cattle in endemic states of India were investigated in association with...  相似文献   

Spatiotemporal separation of groundnut rosette disease agents     

Naidu RA  Kimmins FM  Holt J  Robinson DJ  Deom CM  Subrahmanyam P 《Phytopathology》1999,89(10):934-941

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Short-rotation woody crops and phytoremediation: Opportunities for agroforestry?     

Rockwood  D.L.  Naidu  C.V.  Carter  D.R.  Rahmani  M.  Spriggs  T.A.  Lin  C.  Alker  G.R.  Isebrands  J.G.  Segrest  S.A. 《Agroforestry Systems》2004,61(1-3):51-63

Agroforestry Systems - Worldwide, fuelwood demands, soil and groundwater contamination, and agriculture's impact on nature are growing concerns. Fast growing trees in short rotation woody crop...  相似文献   

Studies on the antioxidant activities of natural vanilla extract and its constituent compounds through in vitro models     

Shyamala BN  Naidu MM  Sulochanamma G  Srinivas P 《Journal of agricultural and food chemistry》2007,55(19):7738-7743

Vanilla extract was prepared by extraction of cured vanilla beans with aqueous ethyl alcohol (60%). The extract was profiled by HPLC, wherein major compounds, viz., vanillic acid, 4-hydroxybenzyl alcohol, 4-hydroxy-3-methoxybenzyl alcohol, 4-hydroxybenzaldehyde and vanillin, could be identified and separated. Extract and pure standard compounds were screened for antioxidant activity using beta-carotene-linoleate and DPPH in vitro model systems. At a concentration of 200 ppm, the extract showed 26% and 43% of antioxidant activity by beta-carotene-linoleate and DPPH methods, respectively, in comparison to corresponding values of 93% and 92% for BHA. Interestingly, 4-hydroxy-3-methoxybenzyl alcohol and 4-hydroxybenzyl alcohol exhibited antioxidant activity of 65% and 45% by beta-carotene-linoleate method and 90% and 50% by DPPH methods, respectively. In contrast, pure vanillin exhibited much lower antioxidant activity. The present study points toward the potential use of vanilla extract components as antioxidants for food preservation and in health supplements as nutraceuticals.  相似文献   

Taste panel assessment and proximate composition of cultured and wild sea scallops, Placopecten magellanicus (Gmelin)     

K.S. Naidu  J.R. Botta 《Aquaculture (Amsterdam, Netherlands)》1978,15(3):243-247

Cage-cultured and wild sea scallops, Placopecten magellanicus, sampled from a location in Placentia Bay, Newfoundland, were assessed by taste panels. Of the five sensory variables investigated (appearance, odor, texture, flavor and overall acceptability), only appearance (P ≤ 0.01) and overall acceptability (P ≤ 0.05) showed significant differences between the two groups. However, no preference was indicated. The adductor muscles were analyzed for total carbohydrate, crude fat, crude protein, moisture and ash. The range for each component was similar (P > 0.05) in both the cultured and wild populations.  相似文献   

Development and evaluation of a rapid absorbed enzyme immunoassay test for the diagnosis of Johne''s disease in cattle   总被引：8，自引：0，他引：8  

JC COX  DP DRANE  SL JONES  S. RIDGE†  AR MILNER† 《Australian veterinary journal》1991,68(5):157-160

An absorbed enzyme immunoassay (EIA) test for Johne's disease in cattle was developed in which absorption of cross-reacting antibodies occurred as a rapid reaction in solution rather than overnight with whole organisms and a subsequent centrifugation step. Total test time was reduced to less than 2 h with a minimum of manipulations. The test was evaluated in cattle herds from Johne's disease-endemic and Johne's disease-free regions of Australia. Specificity was 99.8%. Calculations of sensitivity were affected by the history of the herd under test. However, the EIA detected in excess of 80% of animals before onset of clinical disease and 65% of faecal shedders were EIA positive on, or before, first detection of Mycobacterium paratuberculosis in their faeces. The test should aid epidemiological studies and be a useful tool in the management and control of Johne's disease.  相似文献   

Negative findings from serological studies of equine morbillivirus in the Queensland horse population   总被引：2，自引：0，他引：2  

MP WARD  PF BLACK  AJ CHILDS  FC BALDOCK  WR WEBSTER  BJ RODWELL  SL BROUWER 《Australian veterinary journal》1996,74(3):241-243

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Mercury concentrations in Pacific hake, Merluccius productus (Ayres), as a function of length and latitude     

N H Cutshall  J R Naidu  W G Pearcy 《Science (New York, N.Y.)》1978,200(4349):1489-1491

Mercury concentrations in Pacific hake increase with fish size and with the latitude of collection. While the mercury-size trend is consistent with data for other species, the latitudinal trend is opposite to that reported for other fishes over the same geographical area. Consequently, latitudinal trends of mercury concentrations in fishes do not necessarily indicate trends of mercury concentrations in water. Food habits and metabolism may cause the observed variations.  相似文献   

TAF1B is a TFIIB-like component of the basal transcription machinery for RNA polymerase I     

Naidu S  Friedrich JK  Russell J  Zomerdijk JC 《Science (New York, N.Y.)》2011,333(6049):1640-1642

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