Conductive 3D structure nanofibrous scaffolds for spinal cord regeneration     

Fatemeh Zamani  Mohammad Amani-Tehran  Arash Zaminy  Mohammad-Ali Shokrgozar 《Fibers and Polymers》2017,18(10):1874-1881

The complex nature of spinal cord injuries has provided much inspiration for the design of novel biomaterials and scaffolds which are capable of stimulating neural tissue repair strategies. Recently, conductive polymers have gained much attention for improving the nerve regeneration. In our previous study, a three-dimensional (3D) structure with reliable performance was achieved for electrospun scaffolds. The main purpose in the current study is formation of electrical excitable 3D scaffolds by appending polyaniline (PANI) to biocompatible polymers. In this paper, an attempt was made to develop conductive nanofibrous scaffolds, which can simultaneously present both electrical and topographical cues to cells. By using a proper 3D structure, two kinds of conductive scaffolds are compared with a non-conductive scaffold. The 3D nanofibrous core-sheath scaffolds, which are conductive, were prepared with nanorough sheath and aligned core. Two different sheath polymers, including poly(lactic-co-glycolic acid) PLGA and PLGA/PANI, with identical PCL/PANI cores were fabricated. Nanofibers of PCL and PLGA blends with PANI have fiber diameters of 234±60.8 nm and 770±166.6 nm, and conductivity of 3.17×10^-5 S/cm and 4.29×10^-5 S/cm, respectively. The cell proliferation evaluation of nerve cells on these two conductive scaffolds and previous non-conductive scaffolds (PLGA) indicate that the first conductive scaffold (PCL/ PANI-PLGA) could be more effective for nerve tissue regeneration. Locomotor scores of grafted animals by developed scaffolds showed significant performance of non-conductive 3D scaffolds. Moreover, the animal studies indicated the ability of two new types of conductive scaffolds as spinal cord regeneration candidates.  相似文献   

Effect of transferring lignocellulose-degrading bacteria from termite to rumen fluid of sheep on in vitro gas production,fermentation parameters,microbial populations and enzyme activity          下载免费PDF全文

Ayoub AZIZI  Afrooz SHARIFI  Hasan FAZAELI  Arash AZARFAR  Arjan JONKER  Ali KIANI 《农业科学学报》2020,19(5):1323-1331

The digestive tract of termite(Microcerotermes diversus) contains a variety of lignocellulose-degrading bacteria with exocellulases enzyme activity, not found in the rumen, which could potentially improve fiber degradation in the rumen. The objectives of the current study were to determine the effect of inoculation of rumen fluid(RF) with three species of bacteria isolated from termite digestive tract, Bacillus licheniformis, Ochrobactrum intermedium, and Microbacterium paludicola, on in vitro gas production(IVGP), fermentation parameters, nutrient disappearance, microbial populations, and hydrolytic enzyme activities with fibrous wheat straw(WS) and date leaf(DL) as incubation substrate. Inoculation of RF with either of three termite bacteria increased(P0.05) ammonia-N concentration compared with the control group(free of termite gut bacteria). Termite bacteria inoculation had no effect(P0.05) on gas production characteristics, dry matter, organic matter and neutral detergent fiber disappearance, pH, and concentration and composition of volatile fatty acids. Population of proteolytic bacteria and protozoa, but not cellulolytic bacteria, were increased(P0.05) when RF was inoculated with termite bacteria with both WS and DL substrates. Inoculation of RF with termite bacteria increased protease activity, while activities of carboxymethyl-cellulase, microcrystalline-cellulase, α-amylase and filter paper degrading activity remained unchanged(P0.05). Overall, the results of this study indicated that transferring lignocellulose-degrading bacteria, isolated from digestive tract of termite, to rumen liquid increased protozoa and proteolytic bacteria population and consequently increased protease activity and ammonia-N concentration in vitro, however, no effect on fermentation and fiber degradation parameters were detected. These results suggest that the termite bacteria might be rapidly lysed by the rumen microbes before beneficial effects on the rumen fermentation process could occur.  相似文献   

Contributions to the leafminer fauna (Diptera: Agromyzidae) from Turkey, with four new records     

Emine Çıkman  Hasan Sungur Civelek 《Phytoparasitica》2005,33(4):391-396

In a study carried out during 2002 and 2004 in Diyarbakıir and Mardin provinces, southeast Turkey, specimens were collected twice a month from cultivated and non-cultivated plants. Sixteen leafminer (Diptera: Agromyzidae) species were identified. Among them,Agromyza abiens Zetterstedt, 1848;Napomyza elegans (Meigen, 1830);Phytoliriomyza dorsata (Siebke, 1864); andPhytomyza aquilonia Frey, 1964 are new records for the Turkish leafminer http://www.phytoparasitica.org posting July 24, 2005.  相似文献   

High prevalence and antimicrobial resistance of mecA Staphylococcus aureus in dairy cattle,sheep, and goat bulk tank milk in Jordan     

Obaidat  Mohammad M.  Bani Salman  Alaa E.  Roess  Amira A. 《Tropical animal health and production》2018,50(2):405-412

Tropical Animal Health and Production - The aim of this study was to determine the prevalence and antimicrobial resistance of mecA and mecC methicillin-resistant Staphylococcus aureus (MRSA) in...  相似文献   

Prevalence of avian influenza (H9N2) in commercial quail,partridge, and turkey farms in Iran, 2014–2015     

Mehrabadi  Mohammad Hosein Fallah  Bahonar  Alireza  Mirzaei  Kamran  Molouki  Aidin  Ghalyanchilangeroudi  Arash  Ghafouri  Seyed Ali  Tehrani  Farshad  Lim  Swee Hua Erin 《Tropical animal health and production》2018,50(3):677-682

Tropical Animal Health and Production - Avian influenza virus (AIV) H9N2 subtype is endemic in Iran and causes substantial economic loss to the growing poultry industry within the country. In this...  相似文献   

Molecular analysis of diversity of diploid Triticum species in Iran using ISSR markers     

Seyedeh Shafa Mousavifard  Hojjatollah Saeidi  Mohammad Reza Rahiminejad  Mahdieh Shamsadini 《Genetic Resources and Crop Evolution》2015,62(3):387-394

  相似文献   

Fortification of Bread Wheat Using Synthesized Zn-Glycine and Zn-Alanine Chelates in Comparison with ZnSO4 in a Calcareous Soil     

Atena Mirbolook  Mirhassan Rasouli Sadaghiani  Ebrahim Sepehr  Mohammad Hakimi 《Communications in Soil Science and Plant Analysis》2020,51(8):1048-1064

ABSTRACT

Calcareous soils typically suffer from zinc deficiency and zinc sulfate is incorporated in many cultivated soils. Utilization of ZnSO₄ has some kinds of interaction with soil particles and organic matter. In this study, the efficacy of two znic(Zn)-amino acid chelates (Zn-ACs) i.e., Zn-alanine (Zn-Ala) and Zn-glycine (Zn-Gly) on wheat (Triticum aestivum, cv. N91-8) growth characteristics and zinc concentration in wheat was examined under greenhouse conditions and compared to the a commercial ZnSO₄. Results showed that Zn-Ala and Zn-Gly significantly increased the dry weight and shoot length of wheat in comparison to ZnSO₄ treatment. Soil application of Zn-Amino acid chelates proved to be the most influential source of zinc in increasing wheat growth and yield indices. Number of fertile spikelet and grain yield increased significantly respectively compared to ZnSO₄ treatment. Zn concentration and protein content of wheat grain in Zn-ACs treatment was significantly higher than the ZnSO₄ treatment. Soil application of Zn-ACs caused a significant decrease in the grain phytic acid (PA) concentration and also phytic acid to zinc molar ratio in comparison with ZnSO₄ treatment. According to the results, Zn-ACs could be utilized as a zinc fertilizer source for improving the zinc bioavailability in wheat.  相似文献   

Performance of Bangladesh indigenous rice in a weed infested field and separation of allelopathy from resource competition     

Sheikh Muhammad Masum  Mohammad Amzad Hossain  Hikaru Akamine  Jun‐Ichi Sakagami  Takahiro Ishii  Ichiro Nakamura  Md. Asaduzzaman  Prasanta Chitta Bhowmik 《Weed Biology and Management》2019,19(2):39-50

This study aimed to identify the potential allelopathic indigenous rice (Oryza sativa L. ssp. indica) varieties from Bangladesh using a performance study in a weed‐infested field and to assess the extent of allelopathic interference relative to resource competition in a glasshouse experiment. Six varieties – namely, “Boterswar,” “Goria,” “Biron” and “Kartiksail” as the most allelopathic, “Hashikolmi” as weakly allelopathic and “Holoi” as nonallelopathic – were raised following a nonweed control method. The infestation levels of weed species were calculated using Simpson's Diversity Index (SDI), which ranged from 0.2 to 0.56. However, a significant correlation coefficient (0.87, P < 0.001) was obtained from these field data compared with the root inhibition percentage from the laboratory bioassay, and the “Boterswar” variety was the most allelopathic. The interactions between the allelopathic variety “Boterswar,” weakly allelopathic variety “Hashikolmi” and Echinochloa oryzicola via a target (rice)‐adjacent (E. oryzicola) cogrowth culture were determined in a hydroponic arrangement. The relative competitive intensity (RCI) and the relative neighbor effect (RNE) values showed that the crop–weed interaction was facilitation for “Boterswar” and competition for “Hashikolmi” and E. oryzicola in rice/E. oryzicola cogrowth cultures. The allelopathic effects of “Boterswar” were much higher than the resource competition in rice/E. oryzicola cogrowth cultures. The converse was observed for “Hashikolmi.” Moreover, the mineral content of E. oryzicola was severely affected by “Boterswar”/E. oryzicola cogrowth cultures’ exudate solution. Therefore, the allelopathic potential of “Boterswar” variety might be useful for developing the weed‐suppressing capacity of rice, which will likely have a significant influence on paddy weed control.  相似文献   

The onset of stress response in rainbow trout Oncorhynchus mykiss embryos subjected to density and handling     

Gholamreza Ghaedi  Bahram Falahatkar  Vahid Yavari  Mohammad T. Sheibani  Gholamreza Nikbakht Broujeni 《Fish physiology and biochemistry》2015,41(2):485-493

  相似文献   

Effects of AG1 and AG2 QTLs on Nonstructural Carbohydrate and Seed Management Options for Rice Seedling Growth and Establishment under Flooding Stress     

Mondal Satyen  Jamil Hasan M.  Ahmed Tofayel  Giashuddin Miah M.  C. Sta Cruz Pompe  M. Ismail Abdel 《水稻科学》2020,27(6):515-528

Rice (Oryza sativa) plants acquired excess photosynthates in the form of nonstructural carbohydrates (NSCs) in their stems and grain. Despite keen interest in rice NSC, the dynamics of NSC accumulation, translocation and re-accumulation have not yet been well investigated. AG1 and AG2 QTLs associated with flooding tolerance through catalyzing starch into soluble sugar in germinating seeds. Here we conducted three experiments, greenhouse and field to lay the groundwork for large-scale diversity studies on grain NSC and some agronomic traits under direct-seeded rice (DSR) system, using elite lines incorporating AG1, AG2 and AG1-AG2 QTLs into the popular varieties PSB Rc82 and Ciherang-Sub1 along with the donors Kho Hlan On (AG1) and Ma-Zhan Red (AG2). In germinating seedlings, soluble sugars increased, while starch concentration decreased gradually especially in the tolerant checks and AG1-AG2 introgression lines under flooded soil. Soluble sugar accumulation in stem started to increase from the vegetative stage and peaked at the panicle initiation stage then gradually decreased towards the maturity stage. But Sub1-AG lines had higher sugar and starch concentrations at different growth stages than other genotypes in wet season 2016 and dry season 2017. Plant survival rate was positively correlated with the stem NSC at the early vegetative stage (21 days after sowing), and stem NSC was positively associated with plant height at different growth stages. Among the tested seeding rate, the most suitable seeding rate, 4 g/m² with shallow burial depth (0.5 cm), resulted in better seedling establishment, relatively higher seedling vigor index and higher leaf area index under flooding in DSR system. Introgression of AG1-AG2 QTLs had no any negative impact on nonstructural carbohydrate, germination rate, and growth and biomass production.  相似文献