<Emphasis Type="Italic">Lupinus albus</Emphasis> Conglutin Gamma Modifies the Gene Expressions of Enzymes Involved in Glucose Hepatic Production <Emphasis Type="Italic">In Vivo</Emphasis>     

Ana E. González-Santiago  Belinda Vargas-Guerrero  Pedro M. García-López  Alma L. Martínez-Ayala  José A. Domínguez-Rosales  Carmen M. Gurrola-Díaz 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(2):134-140

Lupinus albus seeds contain conglutin gamma (Cγ) protein, which exerts a hypoglycemic effect and positively modifies proteins involved in glucose homeostasis. Cγ could potentially be used to manage patients with impaired glucose metabolism, but there remains a need to evaluate its effects on hepatic glucose production. The present study aimed to analyze G6pc, Fbp1, and Pck1 gene expressions in two experimental animal models of impaired glucose metabolism. We also evaluated hepatic and renal tissue integrity following Cγ treatment. To generate an insulin resistance model, male Wistar rats were provided 30% sucrose solution ad libitum for 20 weeks. To generate a type 2 diabetes model (STZ), five-day-old rats were intraperitoneally injected with streptozotocin (150 mg/kg). Each animal model was randomized into three subgroups that received the following oral treatments daily for one week: 0.9% w/v NaCl (vehicle; IR-Ctrl and STZ-Ctrl); metformin 300 mg/kg (IR-Met and STZ-Met); and Cγ 150 mg/kg (IR-Cγ and STZ-Cγ). Biochemical parameters were assessed pre- and post-treatment using colorimetric or enzymatic methods. We also performed histological analysis of hepatic and renal tissue. G6pc, Fbp1, and Pck1 gene expressions were quantified using real-time PCR. No histological changes were observed in any group. Post-treatment G6pc gene expression was decreased in the IR-Cγ and STZ-Cγ groups. Post-treatment Fbp1 and Pck1 gene expressions were reduced in the IR-Cγ group but increased in STZ-Cγ animals. Overall, these findings suggest that Cγ is involved in reducing hepatic glucose production, mainly through G6pc inhibition in impaired glucose metabolism disorders.  相似文献   

The value of sea urchin,Lytechinus variegatus,egesta consumed by shrimp,Litopenaeus vannamei     

Karen E. Jensen  J. Christopher Taylor  Robert J. Barry  Louis R. D'Abramo  D. Allen Davis  Stephen A. Watts 《Journal of the World Aquaculture Society》2019,50(3):614-621

Sea urchins produce high‐energy, membrane‐bound fecal pellets that contain residual nutrients and large quantities of microbiota. These egesta are readily consumed by the shrimp, Litopenaeus vannamei. Egesta of the sea urchin, Lytechinus variegatus, were evaluated as a feed supplement or total replacement for a commercial shrimp diet. Shrimp were stocked at 0.49 g ± 0.06 g initial body weight and housed individually in 2.8‐L tanks in a commercial recirculating zebrafish system. Shrimp were assigned to one of six diets: commercial shrimp feed, reference sea urchin feed, collected dried sea urchin egesta, collected wet sea urchin egesta, half ration of shrimp feed and half collected wet sea urchin egesta, and egesta naturally produced by two sea urchins in polyculture. Equivalent dry matter amounts of each diet were proffered to shrimp in each treatment twice daily, except for those that had complete access to natural egesta excreted by sea urchins in polyculture. Sea urchins were proffered a reference sea urchin feed at 2% body weight daily. After 27 days, shrimp proffered collected dried or wet egesta did not differ significantly in percent weight gain and showed the lowest weight gain. The percent weight gain of shrimp fed the commercial shrimp diet did not differ significantly from that of the shrimp fed half commercial shrimp diet and half egesta. The highest weight gain was recorded for those shrimp that consumed the untouched egesta produced by sea urchins in polyculture. These data suggest that consumed egesta have noteworthy nutritional value and therefore would be beneficial to the culture of extractive species in an integrated multitrophic aquaculture system.  相似文献   

Phenological and physiological evaluation of first and second cropping periods of sorghum and maize crops     

Maria Antonia Machado Barbosa  Kacilda Naomi Kuki  Pedro Santos Peno Bengala  Emilly dos Santos Pereira  Angélica Fátima de Barros  Sebastián Giraldo Montoya  Leonardo Duarte Pimentel 《Journal of Agronomy and Crop Science》2020,206(2):263-276

Environmental conditions influence phenology and physiological processes of plants. It is common for maize and sorghum to be sown at two different periods: the first cropping (spring/summer) and the second cropping (autumn/winter). The phenological cycle of these crops varies greatly according to the planting season, and it is necessary to characterize the growth and development to facilitate the selection of the species best adapted to the environment. The aim of this study was to characterize phenological phases and physiological parameters in sorghum and maize plants as a function of environmental conditions from the first cropping and second cropping periods. Two parallel experiments were conducted with both crops. The phenological characterization was based on growth analyses (plant height, leaf area and photoassimilate partitioning) and gas exchange evaluations (net assimilation rate, stomatal conductance, transpiration and water-use efficiency). It was found that the vegetative stage (VS) for sorghum and maize plants was 7 and 21 days, respectively, longer when cultivated during the second cropping. In the first cropping, the plants were taller than in the second cropping, regardless of the crop. The stomatal conductance of sorghum plants fluctuated in the second cropping during the development period, while maize plants showed decreasing linear behaviour. Water-use efficiency in sorghum plants was higher during the second cropping compared with the first cropping. In maize plants, in the second cropping, the water-use efficiency showed a slight variation in relation to the first cropping. It was concluded that the environmental conditions as degree-days, temperature, photoperiod and pluvial precipitation influence the phenology and physiology of both crops during the first and the second cropping periods, specifically cycle duration, plant height, leaf area, net assimilation rate, stomatal conductance and water-use efficiency, indicating that both crops respond differentially to environmental changes during the growing season.  相似文献   

Assessing seed desiccation responses of native trees in the Caribbean     

Mattana  Efisio  Peguero  Brígido  Di Sacco  Alice  Agramonte  Wilvin  Encarnación Castillo  Wilkin Rafael  Jiménez  Francisco  Clase  Teodoro  Pritchard  Hugh W.  Gómez-Barreiro  Pablo  Castillo-Lorenzo  Elena  Terrero Encarnación  Marianny  Way  Michael J.  García  Ricardo  Ulian  Tiziana 《New Forests》2020,51(4):705-721

New Forests - Native trees from the Caribbean were tested for seed desiccation responses, by adapting the “100-seed test” protocol. Ninety-seven seed lots of 91 species were collected...  相似文献   

Prediction of Genetic Gain in Sweet Corn using Selection Indexes     

da Silva  Isadora Gonçalves  Castoldi  Renata  de Oliveira Charlo  Hamilton César  de Souza Miranda  Mateus  Nunes  Thaíssa Dias Cardoso  Costa  Luciene Lacerda  Lemes  Ernane Miranda 《Journal of Crop Science and Biotechnology》2020,23(2):191-196

Journal of Crop Science and Biotechnology - The cultivation of sweet corn is expanding in Brazil, but there are serious constraints about the availability of commercial cultivars. The selection of...  相似文献   

Climate change will affect the ability of forest management to reduce gaps between current and presettlement forest composition in southeastern Canada     

Boulanger  Yan  Arseneault  Dominique  Boucher  Yan  Gauthier  Sylvie  Cyr  Dominic  Taylor  Anthony R.  Price  David T.  Dupuis  Sébastien 《Landscape Ecology》2019,34(1):159-174

Landscape Ecology - Forest landscapes at the boreal–temperate ecotone have been extensively altered. Reducing the gap between current and presettlement forest conditions through...  相似文献   

Proteomic profiles during adventitious rooting of Eucalyptus species relevant to the cellulose industry     

de Almeida  Márcia Rodrigues  Schwambach  Joséli  Silveira  Vanildo  Heringer  Angelo Schuabb  Fett  Janette Palma  Fett-Neto  Arthur G. 《New Forests》2020,51(2):213-241

New Forests - Adventitious rooting (AR) is an obligatory step for vegetative propagation of commercial woody species. Paper industries have interest in Eucalyptus globulus Labill and its hybrids...  相似文献   

The efficiency of nitrogen in cattle manures when applied to a double‐annual forage cropping system          下载免费PDF全文

B. Perramon  À. D. Bosch‐Serra  F. Domingo‐Olivé  J. Boixadera 《Grass and Forage Science》2017,72(4):676-690

The use of cattle manure (CM) for fertilization presents challenges for optimizing nitrogen (N) use. Our work aimed to assess N efficiencies, in a 6‐year experiment with three biennial rotations of four crops: oat–sorghum (first year) and ryegrass–maize (second year) in a rainfed humid Mediterranean area of Spain. Fertilization treatments included the following: control (no N), 250 kg mineral N ha^?1 year^?1 (250MN), three CM rates (supplying 170, 250 and 500 kg N ha^?1 year^?1) and four treatments where the two lowest CM rates were complemented with either 80 or 160 kg mineral N ha^?1 year^?1. Treatments were distributed randomly in each of three blocks. Maximum dry‐matter yield (~44–49 t ha^?1 rotation^?1) was achieved in the third rotation, and only the control and the 170CM yielded significantly less. Within the limitations of the EU Nitrate Directive, the N steady state supply of 170CM always requires a complement of mineral N (80 kg N ha^?1) to maximize N agronomic efficiency. The maximum N‐fertilizer replacement value (250CM vs. 250MN) was 0·67, without significant differences between the two treatments in other N‐related efficiency indexes, which indicates that plants took advantage of residual‐N effects. Nitrogen losses by leaching in the 250CM treatment were around 5–7% of the N applied. This reinforces the sustainability of manure recycling in long cropping seasons.  相似文献   

Whitened kernel surface: A fast and reliable method for assessing Fusarium severity on cereal grains by digital picture analysis     

Marine Ollier  Vincent Talle  Anne‐Laure Brisset  Zo Le Bihan  Simon Duerr  Marc Lemmens  Ellen Goudemand  Olivier Robert  Jean‐Louis Hilbert  Hermann Buerstmayr 《Plant Breeding》2019,138(1):69-81

Fusarium head blight (FHB) is a cereal disease of major importance responsible for yield losses and mycotoxin contaminations in grains. Here, we introduce a new measurement approach to quantify FHB severity on grains based on the evaluation of the whitened kernel surface (WKS) using digital image analysis. The applicability of WKS was assessed on two bread wheat and one triticale grain sample sets (265 samples). Pearson correlation coefficients between Fusarium‐damaged kernels (FDK) and WKS range from r = 0.77 to r = 0.81 and from r = 0.61 to r = 0.86 for the correlation between deoxynivalenol (DON) content and WKS. This new scoring method facilitates fast and reliable assessment of the resistance to kernel infection and shows significant correlation with mycotoxin content. WKS can be automated and does not suffer from the “human factor” inherent to visual scorings. As a low‐cost and fast approach, this method appears particularly attractive for breeding and genetic analysis of FHB resistance where typically large numbers of experimental lines need to be evaluated, and for which WKS is suggested as an alternative to visual FDK scorings.  相似文献   

Evaluation of Particle Size Influence on Proximate Composition,Physicochemical, Techno-Functional and Physio-Functional Properties of Flours Obtained from Persimmon (<Emphasis Type="Italic">Diospyros kaki</Emphasis> Trumb.) Coproducts     

Raquel Lucas-González  Manuel Viuda-Martos  José Ángel Pérez-Álvarez  Juana Fernández-López 《Plant foods for human nutrition (Dordrecht, Netherlands)》2017,72(1):67-73

The aim of the work was to study the influence of particle size in the composition, physicochemical, techno-functional and physio-functional properties of two flours obtained from persimmon (Diospyros kaki Trumb. cvs. ‘Rojo Brillante’ (RBF) and ‘Triump’ (THF) coproducts. The cultivar (RBF and THF) and particle size significantly affected all parameters under study, although depending on the evaluated property, only one of these effects predominated. Carbohydrates (38.07–46.98 g/100 g) and total dietary fiber (32.07–43.57 g/100 g) were the main components in both flours (RBF and THF). Furthermore, insoluble dietary fiber represented more than 68% of total dietary fiber content. All color properties studied were influenced by cultivar and particle size. For both cultivars, the lower particle size, the higher lightness and hue values. RBF flours showed high values for emulsifying activity (69.33–74.00 mL/mL), while THF presented high values for water holding capacity (WHC: 9.47–12.19 g water/g sample). The bile holding capacity (BHC) and fat/oil binding values were, in general, higher in RBF (19.61–12.19 g bile/g sample and 11.98–9.07, respectively) than THF (16.12–12.40 g bile/g sample and 9.78–7.96, respectively). The effect of particle size was really evident in both WHC and BHC. Due to their dietary fiber content, techno-functional and physio-functional properties, persimmon flours seem to have a good profile to be used as potential functional ingredient.  相似文献