鹿肚耳与3种木耳的营养价值评价     

张桐  徐安然  杨迪  钱可晴  Samwel  李晓 《食药用菌》2021,(1)

综述皱木耳Auricularia delicata新品种"鹿肚耳"的形态特征和食用特点,测定其与黑木耳、毛木耳、毛木耳白色变种"玉木耳"人工栽培子实体的基本营养成分、矿质元素含量和氨基酸组成,评价其营养价值。结果表明:鹿肚耳热量235 kJ/100g、蛋白质7.21 g/100g、脂肪1.5 g/100g、碳水化合物48.4 g/100g、总膳食纤维29.3g/100g,含有5种常量元素、5种必需微量元素,其中,硒元素含量是黑木耳的4.6倍;含有人体所需的8种必需氨基酸,氨基酸化学评分(CS)和氨基酸评分(ASS)分别为12和18.86,色氨酸为其限制性氨基酸;蛋白质综合评价低于其他3种木耳。  相似文献   

Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Chlamydomonas reinhardtii Showing High Antimicrobial Activity     

Anguo Li  Ruihao Huang  Chaogang Wang  Qunju Hu  Hui Li  Xiao Li 《Marine drugs》2021,19(5)

Antimicrobial peptides are a class of proteins with antibacterial functions. In this study, the anti-lipopolysaccharide factor isoform 3 gene (ALFPm3), encoding an antimicrobial peptide from Penaeus monodon with a super activity was expressed in Chlamydomonas reinhardtii, which would develop a microalga strain that can be used for the antimicrobial peptide production. To construct the expression cluster, namely pH2A-Pm3, the codon optimized ALFPm3 gene was fused with the ble reporter by 2A peptide and inserted into pH124 vector. The glass-bead method was performed to transform pH2A-Pm3 into C. reinhardtii CC-849. In addition to 8 μg/mL zeocin resistance selection, the C. reinhardtii transformants were further confirmed by genomic PCR and RT-PCR. Western blot analysis showed that the C. reinhardtii-derived ALFPm3 (cALFPm3) was successfully expressed in C. reinhardtii transformants and accounted for 0.35% of the total soluble protein (TSP). Furthermore, the results of antibacterial assay revealed that the cALFPm3 could significantly inhibit the growth of a variety of bacteria, including both Gram-negative bacteria and Gram-positive bacteria at a concentration of 0.77 μM. Especially, the inhibition could last longer than 24 h, which performed better than ampicillin. Hence, this study successfully developed a transgenic C. reinhardtii strain, which can produce the active ALFPm3 driven from P. monodon, providing a potential strategy to use C. reinhardtii as the cell factory to produce antimicrobial peptides.  相似文献   

Exogenous application of allelopathic water extracts helps improving tolerance against terminal heat and drought stresses in bread wheat (Triticum aestivum L. Em. Thell.)          下载免费PDF全文

M. Farooq  F. Nadeem  M. Y. Arfat  M. Nabeel  S. Musadaq  S. A. Cheema  A. Nawaz 《Journal of Agronomy and Crop Science》2018,204(3):298-312

The allelopathic water extracts (AWEs) may help improve the tolerance of crop plants against abiotic stresses owing to the presence of the secondary metabolites (i.e., allelochemicals). We conducted four independent experiments to evaluate the influence of exogenous application of AWEs (applied through seed priming or foliage spray) in improving the terminal heat and drought tolerance in bread wheat. In all the experiments, two wheat cultivars, viz. Mairaj‐2008 (drought and heat tolerant) and Faisalabad‐2008 (drought and heat sensitive), were raised in pots. Both wheat cultivars were raised under ambient conditions in the wire house till leaf boot stage (booting) by maintaining the pots at 75% water‐holding capacity (WHC). Then, managed drought and heat stresses were imposed by maintaining the pots at 35% WHC, or shifting the pots inside the glass canopies (at 75% WHC), at booting, anthesis and the grain filling stages. Drought stress reduced the grain yield of wheat by 39%–49%. Foliar application of AWEs improved the grain yield of wheat by 26%–31%, while seed priming with AWEs improved the grain yield by 18%–26%, respectively, than drought stress. Terminal heat stress reduced the grain yield of wheat by 38%. Seed priming with AWEs improved the grain yield by 21%–27%; while foliar application of AWEs improved the grain yield by 25%–29% than the heat stress treatment. In conclusion, the exogenous application of AWEs improved the stay green, accumulation of proline, soluble phenolics and glycine betaine, which helped to stabilize the biological membranes and improved the tolerance against terminal drought and heat stresses.  相似文献   

Impact of grassland contract policy on soil organic carbon losses from alpine grassland on the Qinghai–Tibetan Plateau          下载免费PDF全文

J. Cao  Y. Gong  E. T. Yeh  N. M. Holden  J. F. Adamowski  R. C. Deo  M. Liu  J. Zhou  J. Zhang  W. Zhang  S. Zhang  D. Sheng  S. Yang  X. Xu  M. Li  Q. Feng 《Soil Use and Management》2017,33(4):663-671

Carbon storage in the soils on the Qinghai–Tibetan Plateau plays a very important role in the global carbon budget. In the 1990s, a policy of contracting collective grasslands to smaller units was implemented, resulting in a change from the traditional collective grassland management to two new management patterns: a multi‐household management pattern (MMP: grassland shared by several households without enclosures) and a single‐household management pattern (SMP: grassland enclosed and used by only one household). In 2016, 50 MMP and 54 SMP winter pastures on the Qinghai–Tibetan Plateau were sampled to assess the differences in soil organic carbon (SOC) between the two management patterns. Results showed that average SOC was significantly greater under MMP than under SMP, with an estimated 0.41 Mg C/ha/yr lost due to SMP following the new grassland contract. Based on the government's grassland policy, four grassland utilization scenarios were developed for both summer and winter pastures. We found that if the grassland were managed under SMP, likely C losses ranged between 0.31 × 10⁷ and 6.15 × 10⁷ Mg C/yr across the Qinghai–Tibetan Plateau relative to MMP, which more closely resembles pre‐1990s grassland management. Previous estimates of C losses have only considered land use change (with cover change) and ignored the impacts driven by land management pattern changes (without cover change). The new data suggest that C losses from the Qinghai–Tibetan Plateau are greater than previously estimated, and therefore that the grassland contract policy should be reviewed and SMP households should be encouraged to reunite into the MMP. These findings have potential implications for land management strategies not only on the Qinghai–Tibetan Plateau but also other grazing regions globally where such practices may exist.  相似文献   

优质、高产转基因抗虫棉杂交种苏杂668的选育与栽培技术   总被引：1，自引：1，他引：0  

肖松华  刘剑光  赵君  吴巧娟  徐剑文 《中国棉花》2017,44(10):17-18

本文概述了苏杂668的选育过程，阐明了苏杂668的特征特性、产量水平、纤维品质和抗性表现，并提出其高产栽培技术措施。  相似文献   

优质、低酚棉花新品种苏棉158的选育及其高产栽培技术     

肖松华  赵君  徐剑文  刘剑光  吴巧娟 《中国棉花》2020,47(12):19-19

介绍了苏棉158的选育过程、特征特性、产量水平、纤维品质和抗病性、抗虫性及高产高效栽培技术。  相似文献   

Spatio-temporal pattern evolution and spatial spillover effect of green development efficiency: Evidence from Shandong Province,China     

Fuyou Guo  LianJun Tong  Limeng Xu  Xiao Lu  Yanwen Sheng 《Growth and change》2020,51(1):382-401

Improving green development efficiency is urgently required, yet challenging, since it comprehensively reflects the bidirectional evolutionary relationship between regional development and resource and environmental consumption. The issue of regional green development efficiency has become a key topic; however, where the increase in efficiency originates and its spatial spillover effect remains unclear to date. Therefore, the spatial spillover effect of the green development efficiency of Shandong Province was quantified through the slack variable (SBM)-Undesirable and Spatial Durbin model. The green development efficiency showed clear spatial differentiation in Shandong Province. This was more obvious than the polarized development trend of the provincial capital circle that has Jinan at its core and the eastern coastal region with Qingdao at its core. Green development efficiency has a significant spatial spillover effect. The regression coefficient of the direct effect of the economic development level is positive. The regression coefficient of the indirect effect of the marketization level and government financial support level is negative. Science and technology played a positive role in the promotion of the green development efficiency of local and neighboring cities. This study contributes empirical evidence to the green development efficiency associated with regional development and resource and environment consumption of Shandong Province.  相似文献   

海贝柱自动晾晒设备设计     

刘晓  侯加林  张观山  周伟 《渔业现代化》2017,44(5)

针对当前传统人工晾晒海贝柱效率低下、容易造成污染等问题。设计了海贝柱自动晾晒机械,采用阶梯式晾晒生产线对湿海贝柱分级晾晒,多条晾晒生产线分时、高效的工作,机器上方设有遮板以保证晾晒作业不被污染而且不受天气条件的影响。整机采用stm32单片机、传感器等技术实现自动化作业,机器控制系统监测到生产线发生故障或海贝柱出现堆积时会立即停止工作以便于检查故障、减少损失。试验结果表明:机械晾晒不会造成海贝柱内的营养成分的流失,机械晾晒海贝柱的干基含水率达到30%仅需12h,机械晾晒海贝柱能节省大量的干燥时间,显著的提高了晾晒效率,避免了人工晾晒过程导致的二次污染和损失。该研究为海贝柱自动晾晒机械的研制提供了技术参考。  相似文献   

捕食线虫性真菌的分离培养及分布规律   总被引：6，自引：1，他引：5  

杨晓野  杨莲茹  刘珍莲  董世娟  考桂兰 《中国兽医学报》2003,23(4):344-347

对自然界中的捕食线虫性真菌进行了分离和种属区分，并对分布规律进行了研究。从土壤、粪便等材料中获得了2类活性较强的捕食线虫性菌株：即套捕型(hooping)捕食线虫性真菌和粘捕型(sticking)捕食线虫性真菌。主要的代表种类有3种。这些捕食线虫性真菌在捕食性结构分生孢子形态及某些生物学特性上有一定差异。套捕型捕食线虫性真菌代表种为少孢节丛孢菌(Arthrobotrys oligospara)和梨形指环菌(Dactylaria pyriformis)，它们以菌环、菌网作为捕食性结构(器官)，以套捕方式杀灭线虫幼虫。梨形指环菌可产生多量厚垣孢子(chlamyalospore)。粘捕型捕食线虫性真菌代表种为纺锤隔指孢菌(Dactylella ellipsospora)。可形成菌结捕食线虫性结构，以粘捕的方式杀灭线虫幼虫。结果表明：捕食线虫性真菌在土壤和家畜粪便中的分离率是40．41％；此类菌适合于生存在阴暗、潮湿、富含腐植质的环境中。在温暖季节时，采用0．4g／L玉米粉琼脂培养基(CMA)易于对其进行分离培养。  相似文献   

Staining pattern of the brush border and detection of cytoplasmic granules in the uriniferous tubules of female DBA/2Cr mouse kidney: comparison among various fixations and stains.     

A Yabuki  S Suzuki  M Matsumot  M Kurohmaru  Y Hayashi  H Nishinakagawa 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2001,63(12):1339-1342

The proximal straight tubules of the female mouse kidney exhibit heavy periodic acid Schiff (PAS) staining in their brush borders and numerous cytoplasmic granules. In the present study, the female DBA/2Cr mouse kidney was examined, using various fixatives (formalin, PFA, PLP, Zamboni's, Bouin, or Carnoy solution) and various staining methods (HE, PAS, alcian blue, periodic acid methenamine-silver (PAM), toluidine blue, azan, or Congo red). Under azan and PAM, the staining pattern of the brush border was similar to that of PAS, and few effects of the fixative were observed. Cytoplasmic granules were clearly detected with PAM as well as PAS. However, these granules were not detected with Carnoy solution. Furthermore, distribution of granules differed between PAS and PAM.  相似文献