排序方式： 共有33条查询结果，搜索用时 15 毫秒
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Hiroshi BANNAI Manabu NEMOTO Koji TSUJIMURA Takashi YAMANAKA Ken MAEDA Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):309-311
To increase the sensitivity of an enzyme-linked immunosorbent assay (ELISA) for equine herpesvirus type 4
(EHV-4) that uses a 12-mer peptide of glycoprotein G (gG4-12-mer: MKNNPIYSEGSL) [4], we used a longer peptide consisting of a 24-mer repeat sequence (gG4-24-mer:
MKNNPIYSEGSLMLNVQHDDSIHT) as an antigen. Sera of horses experimentally infected with EHV-4 reacted much more
strongly to the gG4-24-mer peptide than to the gG4-12-mer peptide. We used peptide ELISAs to test paired sera
from horses naturally infected with EHV-4 (n=40). gG4-24-mer ELISA detected 37 positive samples (92.5%),
whereas gG4-12-mer ELISA detected only 28 (70.0%). gG4-24-mer ELISA was much more sensitive than gG4-12-mer
ELISA. 相似文献
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Wenxi CHEN Xin ZHU Tetsu NEMOTO Toshio KOBAYASHI Toshiyuki SAITO 《Animal Science Journal》2004,75(5):471-478
The fetal heart rate is indispensable for monitoring the health of unborn cattle fetuses. To monitor the fetal heart rate, a method employing independent component analysis (ICA) to extract the fetal electrocardiogram (fECG) from potentials measured on the maternal body surface and composed of a mixture of the maternal ECG (mECG), fECG, baseline drift and noise is described. A mixing of the raw data was simplified using a linear time‐invariant model. To separate the fECG from the mECG, baseline drift, and noise, an ICA strategy was applied, using a hyperbolic tangent as the contrast function and treating mutual information with the minimization principle to find the optimum demixing matrix to derive the fECG from the measured signals. After the feasibility of this method was shown on simulated signals obtained by randomly mixing pure fECG, pure mECG, low frequency sinusoidal drift and noise, real signals from three cloned pregnant Holstein cows with 157, 177 and 224‐day gestation periods were used to verify the separation method. The results show that the fECG, mECG, low‐frequency sinusoidal drift and noise can be clearly segregated in simulations, and that the fECG, mECG, baseline drift and noise can be successfully derived from real signals. The ICA approach has great potential in effectively detecting the fECG from maternal body surface potentials. 相似文献
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Ko NAKASUMI Naoki YAMAMOTO Taro TAKAMI Harumichi ITOH Kazuhito ITAMOTO Hiro HORIKIRIZONO Toshie ISERI Munekazu NAKAICHI Yuki NEMOTO Hiroshi SUNAHARA Kenji TANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):114
Transcatheter arterial embolization (TAE) and transcatheter arterial chemoembolization (TACE) are standard treatments for advanced hepatocellular carcinoma (HCC) and particularly for unresectable tumors or liver metastases in humans. However, reports on TACE used in veterinary medicine are few. This study aimed to evaluate the feasibility and safety of drug-eluting bead transarterial chemoembolization (DEB-TACE). We performed DEB-TACE in four clinically normal dogs and pharmacokinetically compared the results against hepatic arterial infusion (HAI) of cisplatin in two dogs. Drug-eluting beads (DEB) loaded with cisplatin were injected through a microcatheter for selective embolization of the left hepatic artery. After embolization, computed tomography (CT) images and histological examination findings were obtained during a 4-week observation period. Serum platinum concentrations were measured to evaluate cisplatin after each procedure. Biochemical analysis was performed during a 12-week observation period. Embolization was successful in all dogs, and there were no clinically apparent abnormalities. Embolization was confirmed up to 4 weeks after DEB-TACE in two of the four dogs and up to 1 week in the other two dogs using postoperative CT images. Cisplatin was not detected in peripheral veins in all dogs after DEB-TACE, but it was detected in trace amounts after HAI. DEB-TACE using cisplatin was safe and well tolerated by normal dogs. DEB-TACE may be useful in terms of determining systemic toxicity and drug concentration within tumors. 相似文献
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SHIN‐ICHI ITO MICHIO J. KISHI YUTAKA KURITA YOSHIOKI OOZEKI YASUHIRO YAMANAKA BERNARD A. MEGREY FRANCISCO E. WERNER 《Fisheries Oceanography》2004,13(Z1):111-124
A fish bioenergetics model coupled with an ecosystem model was developed to reproduce the growth of Pacific saury. The model spatially covers three different oceanographic spatial domains corresponding to the Kuroshio, Oyashio, and interfrontal (mixed water) regions. In this coupled model, three (small, large, and predatory) zooplankton densities which were derived from the lower trophic level ecosystem model were input to the bioenergetics model of saury as the prey densities. Although certain model parameters were imposed from other species’ bioenergetics, several model parameters were estimated from observational data specific to Pacific saury. The integrated model results reproduced appropriate growth rates of Pacific saury. Model sensitivities to water temperature and prey density are examined and observational methods to evaluate the model parameters are discussed. 相似文献
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Koji TSUJIMURA Harutaka MURASE Hiroshi BANNAI Manabu NEMOTO Takashi YAMANAKA Takashi KONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1545-1548
We investigated the influences of various reaction conditions on equine
herpesvirus type 1 (EHV-1) disinfection by 5 commercial disinfectants (3 quaternary
ammonium compounds [QACs] and 2 chlorine-based disinfectants) and 1 anionic surfactant.
QACs at their highest recommended concentrations had no virucidal effect on EHV-1 with a
10-min reaction time at 0°C or a 1-min reaction time at room temperature. Chlorine-based
disinfectants achieved EHV-1 disinfection with a 10-min reaction time at −10°C or a 30-sec
reaction time at room temperature. In the presence of 5% fetal bovine serum, QACs (except
for benzalkonium chloride) showed more stable virucidal effects than did chlorine-based
disinfectants. The virucidal effect of the anionic surfactant was almost equivalent to
that of the QACs. 相似文献