Evaluating environmental impacts of the Japanese beef cow–calf system by the life cycle assessment method     

Akifumi OGINO  Hideki ORITO  Kazuhiro SHIMADA  Hiroyuki HIROOKA 《Animal Science Journal》2007,78(4):424-432

The objectives of this study were to evaluate the environmental impacts of a beef cow–calf system using a life cycle assessment (LCA) method and to investigate the effects of scenarios to reduce environmental impacts on the LCA results. The functional unit was defined as one marketed beef calf, and the processes associated with the cow–calf life cycle, such as feed production, feed transport, animal management, the biological activity of the animal and the treatment of cattle waste were included in the system boundary. The present results showed that the total contributions of one beef calf throughout its life cycle to global warming, acidification, eutrophication and energy consumption were 4550 kg of CO₂ equivalents, 40.1 kg of SO₂ equivalents, 7.0 kg of phosphate (PO₄) equivalents and 16.1 GJ, respectively. The contribution of each process to the total environmental impact in each environmental impact category showed a similar tendency to the contribution of each process in each environmental category reported in the case of the beef fattening system as a whole. The results from this analysis showed that shortening calving intervals by 1 month reduced environmental impacts by 5.7–5.8% in all the environmental impact categories examined in the current study, and increasing the number of calves per cow also reduced environmental impacts in all the categories, although the effects were smaller.  相似文献   

Development of a monoclonal antibody for the detection of anti-canine CD20 chimeric antigen receptor expression on canine CD20 chimeric antigen receptor-transduced T cells     

Osamu SAKAI  Shoji OGINO  Toshihiro TSUKUI  Masaya IGASE  Takuya MIZUNO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(10):1495

Chimeric antigen receptor (CAR) CAR-T cell therapy targeting CD20 can be a novel adoptive cell therapy for canine patients with B-cell malignancy. After injection of the CAR-T cells in vivo, monitoring circulating CAR-T cells is essential to prove in vivo persistence of CAR-T cells. In this study, we developed a novel monoclonal antibody against canine CD20 CAR, whose single-chain variable fragment was derived from the our previously reported anti-canine CD20 therapeutic antibody. Furthermore, we proved that this monoclonal antibody can detect therapeutic anti-canine CD20 chimeric antibody in the serum from healthy beagle dogs injected with the therapeutic antibody for safety study. This monoclonal antibody is a useful tool for monitoring both canine CD20-CAR-T cells and anti-canine CD20 therapeutic antibody for canine lymphoma.  相似文献   

The effect of lighting conditions on the rhythmicity of growth hormone secretion in Holstein steers     

Etsuko KASUYA  Shiro KUSHIBIKI  Ken‐ichi YAYOU  Koichi HODATE  Mizuna OGINO  Madoka SUTOH 《Animal Science Journal》2012,83(3):238-244

Growth hormone (GH) secretion regularity and the effects of lighting condition and GH‐releasing hormone (GHRH) on GH release were determined in steers. First, steers were kept under 12:12 L : D conditions (light: 06.00–18.00 hours). The animals were then subjected to a 1‐h advancement in lighting on/off conditions (05.00 and 17.00 hours, respectively). Blood was sampled for 24 h at 1‐h interval on the seventh day of each condition. Second, GHRH was injected intravenously (IV) at 12.00 and 00.00 hours under 12:12 L : D and blood was sampled at 15‐min interval for 4‐h (1 h before and 3 h after the injection). Plasma GH concentrations were measured by a radioimmunoassay. Periodicity of GH secretory profile was calculated by power spectrum analysis using the maximum entropy method. Plasma GH concentrations showed a characteristic pattern consisting of four distinct peaks. Mean periodicity of GH secretory profile was 5.7 h, and it was not altered by any change in lighting conditions. IV injection of GHRH increased GH secretion during the day and night. The increase in GH secretory volume after GHRH injection during the night was equal to that during the day. The present results suggest that GH secreted from the anterior pituitary have regularity in steers.  相似文献   

Application of highly differentiated SNPs between Japanese Black and Holstein to a breed assignment test between Japanese Black and F1 (Japanese Black x Holstein) and Holstein     

Shota NISHIMURA  Toshio WATANABE  Atsushi OGINO  Kazuhiro SHIMIZU  Mitsuo MORITA  Yoshikazu SUGIMOTO  Akiko TAKASUGA 《Animal Science Journal》2013,84(1):1-7

Two taurine breeds, Japanese Black and Holstein, established from geographically distant origins and selected for different uses, beef and dairy, were extensively genotyped using a genome‐wide single nucleotide polymorphism (SNP) chip with more than 1000 animals of each breed. The genetic structure was examined by principal component analysis, in which the first principal component clearly separated the two breeds and explained more than 15% of the variance. Highly differentiated SNPs were detected throughout the genome, some of which were clustered within small regions on BTA4 (79.2–79.7 Mb, Btau4.0) and BTA26 (22.2–23.6 Mb). A breed assignment test was developed using 18 highly differentiated SNPs to distinguish Japanese Black from F₁ (Japanese Black × Holstein) and Holstein. The error rate that an F₁ or Holstein animal is misjudged as Japanese Black was expected to be < 0.8%, while the error rate that a Japanese Black animal is misjudged as F₁ or Holstein was expected to be < 0.001%. This test provides a reliable and powerful method to detect breed label falsification in retail beef.  相似文献   

Plasma cortisol and prolactin secretion rhythms in cattle under varying external environments and management techniques     

Mizuna Ogino  Akihiro Matsuura  Atusi Yamazaki  Mami Irimajiri  Yoshihiro Suzuki  Shiro Kushibiki  Hroyuki Singu  Etsuko Kasuya  Yoshihisa Hasegawa  Koichi Hodate 《Animal Science Journal》2014,85(1):58-68

The secretion rhythms of plasma cortisol (CORT) and prolactin (PRL), hormones related to stress responsiveness and biological rhythm and controlled by light and temperature, were investigated under varying external environments and different management techniques. Serial blood samples were collected from female cattle reared in free‐stall and freely fed (FF) conditions (n = 4) or in tie‐stall and restricted feeding (RF) conditions (hay and concentrate twice daily, n = 4). Plasma CORT and PRL concentrations, eating behavior, and environmental parameters were analyzed. Cyclic patterns for each parameter were examined using spectral analysis, and correlations between CORT, PRL and other parameters were investigated using cross‐spectral analysis. Under FF conditions, CORT secretion was not related to the lighting intensity and eating behavior. However, under RF conditions, the CORT secretion rhythm showed a distinct correlation with lighting intensity and eating behavior. Under FF conditions, the PRL secretion rhythm was similar in all seasons. However, under RF conditions, the PRL rhythm oscillated with high frequency in summer and low frequency in winter, indicating a seasonal change in rhythm. The present study demonstrates that hormone secretion rhythms change under different environments and management techniques.  相似文献   

Biological rhythms related to metabolism in Japanese Shorthorn cattle under varying environments and management techniques     

Mizuna Ogino  Akihiro Matsuura  Atusi Yamazaki  Mami Irimajiri  Hideyuki Takahashi  Tokushi Komatsu  Shiro Kushibiki  Hiroyuki Shingu  Etsuko Kasuya  Yoshihisa Hasegawa  Koichi Hodate 《Animal Science Journal》2013,84(6):513-526

Plasma insulin (INS), thyroxin (T₄), glucose (GLU), non‐esterified fatty acid (NEFA), blood urea nitrogen (BUN), rectal temperature (RT) and eating behavior were evaluated in Japanese Shorthorn cattle under varying external environments and management techniques. Serial blood collection and assessments of RT and eating behavior were performed over 48 h in the spring, summer, autumn and winter in four female cattle reared under either free‐stall and ad libitum feeding (FA) conditions or tie‐stall and restricted feeding (TR) conditions. Cycle patterns for each parameter were examined using spectral analysis, and correlations between parameters were investigated using cross‐spectral analysis. Rhythms for all parameters, except eating behavior and T₄, did not differ significantly among the varied external environments and between management techniques, although seasonal differences in the concentration or value of parameters were observed. An approximate 3‐ or 4‐h rhythm cycle detected in T₄, GLU, NEFA, BUN, and RT might be the common metabolic rhythm. Under both conditions, the metabolite levels showed strong correlations with eating behavior. Moreover, GLU positively correlated with INS at lag time of 0 h, as did eating behavior and RT.  相似文献   

Plasma melatonin secretion rhythms in cattle under varying seasonal conditions     

Mizuna Ogino  Akihiro Matsuura  Atusi Yamazaki  Mami Irimajiri  Shiro Kushibiki  Hiroyuki Shingu  Etsuko Kasuya  Yoshihisa Hasegawa  Koichi Hodate 《Animal Science Journal》2013,84(3):253-257

The purpose of the present study was to investigate the secretion cycles of melatonin (MEL) in cattle over the course of four seasons. Four female Japanese Shorthorn cattle under free‐stall and ad libitum feeding conditions were used, and plasma MEL concentrations were measured over a 48 h period at 1 h intervals. The time‐series data were analyzed by spectral analysis, and the cycle hour was determined. Data indicated that the secretion cycle for MEL was approximately 23.5 h for all four seasons. The area under the curve of MEL from start to end of experiment for 48 h did not differ significantly among the four seasons. However, the duration of high MEL secretion which defined the duration time of the values were more than 10 pg/mL and differed significantly among the four seasons. In conclusion, this study, which was the first to use spectral analysis to evaluate the cyclic rhythm of MEL in cattle, revealed that MEL secretion cycles did not differ among the seasons. These findings are inconsistent with previous study results in that previous reports suggested that the MEL secretion cycle differed under different lighting conditions.  相似文献   

Environmental impacts of a Japanese dairy farming system using whole‐crop rice silage as evaluated by life cycle assessment     

Akifumi OGINO  Motohiko ISHIDA  Tetsuya ISHIKAWA  Atsuo IKEGUCHI  Miyoko WAKI  Hiroshi YOKOYAMA  Yasuo TANAKA  Hiroyuki HIROOKA 《Animal Science Journal》2008,79(6):727-736

The objectives of this study were to assess and compare the environmental impacts of two types of dairy farming systems, one of which makes use of whole‐crop rice silage and the other of which is conventional, using life cycle assessment (LCA). The functional unit was defined as 1 kg of 4% fat‐corrected milk (FCM). The processes associated with the dairy farming life cycle, such as feed production, feed transport, animal management including biological activity of the animal, and waste treatment were included within the system's boundaries. Environmental impacts of the rice silage‐using and conventional dairy farming systems were 987 and 972 g CO₂ equivalents for global warming, 6.87 and 7.13 g SO₂ equivalents for acidification, 1.19 and 1.23 g PO₄ equivalents for eutrophication, and 5.53 and 5.81 MJ for energy consumption, respectively. Our results suggest that the dairy farming system using rice silage in Japan has smaller environmental impacts for acidification, eutrophication, and energy consumption, and a larger impact for global warming compared with conventional farming. Further interpretation integrating these impact categories suggested 1.1% lower environmental impact of the rice silage‐using dairy farming system as a whole.  相似文献